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Protein

N-acyl-L-amino acid amidohydrolase

Gene

amaA

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes most efficiently N-acetyl derivatives of aromatic amino acids but is also active on other amino acids. L-stereospecific.

Catalytic activityi

An N-acyl-aliphatic-L-amino acid + H2O = an aliphatic L-amino acid + a carboxylate.
An N-acetyl-L-cysteine-S-conjugate + H2O = an L-cysteine-S-conjugate + acetate.

Cofactori

GO - Molecular functioni

  1. aminoacylase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Cobalt

Names & Taxonomyi

Protein namesi
Recommended name:
N-acyl-L-amino acid amidohydrolase (EC:3.5.1.14)
Short name:
L-aminoacylase
Gene namesi
Name:amaA
Synonyms:ama
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 370370N-acyl-L-amino acid amidohydrolasePRO_0000061949Add
BLAST

Interactioni

Subunit structurei

Homotetramer.

Structurei

3D structure databases

ProteinModelPortaliP37112.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M20 family.Curated

Family and domain databases

Gene3Di3.30.70.360. 1 hit.
InterProiIPR017439. Amidohydrolase.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
[Graphical view]
PfamiPF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
[Graphical view]
PIRSFiPIRSF005962. Pept_M20D_amidohydro. 1 hit.
SUPFAMiSSF55031. SSF55031. 1 hit.
TIGRFAMsiTIGR01891. amidohydrolases. 1 hit.

Sequencei

Sequence statusi: Complete.

P37112-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKEEIKRLV DEVKTDVIAW RRHLHAHPEL SFQEEKTAQF VYETLQSFGH
60 70 80 90 100
LELSRPTKTS VMARLIGQQP GRVVAIRADM DALPIQEENT FEFASKNPGV
110 120 130 140 150
MHACGHDGHT AMLLGTAKIF SQLRDDIRGE IRFLFQHAEE LFPGGAEEMV
160 170 180 190 200
QAGVMDGVDV VIGTHLWSPL ERGKIGIVYG PMMAAPDRFF IRIIGKGGHG
210 220 230 240 250
AMPHQTIDAI AIGAQVVTNL QHIVSRYVDP LEPLVLSVTQ FVAGTAHNVL
260 270 280 290 300
PGEVEIQGTV RTFDETLRRT VPQWMERIVK GITEAHGASY EFRFDYGYRP
310 320 330 340 350
VINYDEGDPR HGGNGVRAVR RRGSGPLETE HGRRRFLRLF AKSARQLFLR
360 370
RRGQCRKRHR LPAPPPALYD
Length:370
Mass (Da):41,676
Last modified:October 1, 1994 - v1
Checksum:i479AF1559DB527FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74289 Genomic DNA. Translation: CAA52342.1.
Y08753 Genomic DNA. Translation: CAA70000.1.
PIRiI40358.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74289 Genomic DNA. Translation: CAA52342.1.
Y08753 Genomic DNA. Translation: CAA70000.1.
PIRiI40358.

3D structure databases

ProteinModelPortaliP37112.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.30.70.360. 1 hit.
InterProiIPR017439. Amidohydrolase.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
[Graphical view]
PfamiPF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
[Graphical view]
PIRSFiPIRSF005962. Pept_M20D_amidohydro. 1 hit.
SUPFAMiSSF55031. SSF55031. 1 hit.
TIGRFAMsiTIGR01891. amidohydrolases. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Gene cloning, sequence analysis, purification, and characterization of a thermostable aminoacylase from Bacillus stearothermophilus."
    Sakanyan V., Desmarez L., Legrain C., Charlier D.R.M., Mett I., Kochikyan A., Savchenko A., Boyen A., Falmagne P., Pirard A., Glansdorff N.
    Appl. Environ. Microbiol. 59:3878-3888(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10, CHARACTERIZATION.
    Strain: NCIMB 8224 / CCM 2186 / VKM B-718.
  2. "Two amino acid amidohydrolase genes encoding L-stereospecific carbamoylase and aminoacylase are organized in a common operon in Bacillus stearothermophilus."
    Batisse N., Weigel P., Lecocq M., Sakanyan V.
    Appl. Environ. Microbiol. 63:763-766(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: NCIMB 8224 / CCM 2186 / VKM B-718.

Entry informationi

Entry nameiAMAA_GEOSE
AccessioniPrimary (citable) accession number: P37112
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 26, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.