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P37112 (AMAA_GEOSE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
N-acyl-L-amino acid amidohydrolase

Short name=L-aminoacylase
EC=3.5.1.14
Gene names
Name:amaA
Synonyms:ama
OrganismGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifier1422 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Protein attributes

Sequence length370 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes most efficiently N-acetyl derivatives of aromatic amino acids but is also active on other amino acids. L-stereospecific.

Catalytic activity

An N-acyl-aliphatic-L-amino acid + H2O = an aliphatic L-amino acid + a carboxylate.

An N-acetyl-L-cysteine-S-conjugate + H2O = an L-cysteine-S-conjugate + acetate.

Cofactor

Cobalt.

Subunit structure

Homotetramer.

Sequence similarities

Belongs to the peptidase M20 family.

Ontologies

Keywords
   LigandCobalt
   Molecular functionHydrolase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Molecular_functionaminoacylase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 370370N-acyl-L-amino acid amidohydrolase
PRO_0000061949

Sequences

Sequence LengthMass (Da)Tools
P37112 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 479AF1559DB527FB

FASTA37041,676
        10         20         30         40         50         60 
MTKEEIKRLV DEVKTDVIAW RRHLHAHPEL SFQEEKTAQF VYETLQSFGH LELSRPTKTS 

        70         80         90        100        110        120 
VMARLIGQQP GRVVAIRADM DALPIQEENT FEFASKNPGV MHACGHDGHT AMLLGTAKIF 

       130        140        150        160        170        180 
SQLRDDIRGE IRFLFQHAEE LFPGGAEEMV QAGVMDGVDV VIGTHLWSPL ERGKIGIVYG 

       190        200        210        220        230        240 
PMMAAPDRFF IRIIGKGGHG AMPHQTIDAI AIGAQVVTNL QHIVSRYVDP LEPLVLSVTQ 

       250        260        270        280        290        300 
FVAGTAHNVL PGEVEIQGTV RTFDETLRRT VPQWMERIVK GITEAHGASY EFRFDYGYRP 

       310        320        330        340        350        360 
VINYDEGDPR HGGNGVRAVR RRGSGPLETE HGRRRFLRLF AKSARQLFLR RRGQCRKRHR 

       370 
LPAPPPALYD 

« Hide

References

[1]"Gene cloning, sequence analysis, purification, and characterization of a thermostable aminoacylase from Bacillus stearothermophilus."
Sakanyan V., Desmarez L., Legrain C., Charlier D.R.M., Mett I., Kochikyan A., Savchenko A., Boyen A., Falmagne P., Pirard A., Glansdorff N.
Appl. Environ. Microbiol. 59:3878-3888(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10, CHARACTERIZATION.
Strain: NCIMB 8224 / CCM 2186 / VKM B-718.
[2]"Two amino acid amidohydrolase genes encoding L-stereospecific carbamoylase and aminoacylase are organized in a common operon in Bacillus stearothermophilus."
Batisse N., Weigel P., Lecocq M., Sakanyan V.
Appl. Environ. Microbiol. 63:763-766(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: NCIMB 8224 / CCM 2186 / VKM B-718.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X74289 Genomic DNA. Translation: CAA52342.1.
Y08753 Genomic DNA. Translation: CAA70000.1.
PIRI40358.

3D structure databases

ProteinModelPortalP37112.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.70.360. 1 hit.
InterProIPR017439. Amidohydrolase.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
[Graphical view]
PfamPF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
[Graphical view]
PIRSFPIRSF005962. Pept_M20D_amidohydro. 1 hit.
SUPFAMSSF55031. SSF55031. 1 hit.
TIGRFAMsTIGR01891. amidohydrolases. 1 hit.
ProtoNetSearch...

Entry information

Entry nameAMAA_GEOSE
AccessionPrimary (citable) accession number: P37112
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: February 19, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries