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P37112

- AMAA_GEOSE

UniProt

P37112 - AMAA_GEOSE

Protein

N-acyl-L-amino acid amidohydrolase

Gene

amaA

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    • Comment

    Functioni

    Hydrolyzes most efficiently N-acetyl derivatives of aromatic amino acids but is also active on other amino acids. L-stereospecific.

    Catalytic activityi

    An N-acyl-aliphatic-L-amino acid + H2O = an aliphatic L-amino acid + a carboxylate.
    An N-acetyl-L-cysteine-S-conjugate + H2O = an L-cysteine-S-conjugate + acetate.

    Cofactori

    Cobalt.

    GO - Molecular functioni

    1. aminoacylase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Cobalt

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-acyl-L-amino acid amidohydrolase (EC:3.5.1.14)
    Short name:
    L-aminoacylase
    Gene namesi
    Name:amaA
    Synonyms:ama
    OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
    Taxonomic identifieri1422 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 370370N-acyl-L-amino acid amidohydrolasePRO_0000061949Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.

    Structurei

    3D structure databases

    ProteinModelPortaliP37112.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M20 family.Curated

    Family and domain databases

    Gene3Di3.30.70.360. 1 hit.
    InterProiIPR017439. Amidohydrolase.
    IPR002933. Peptidase_M20.
    IPR011650. Peptidase_M20_dimer.
    [Graphical view]
    PfamiPF07687. M20_dimer. 1 hit.
    PF01546. Peptidase_M20. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005962. Pept_M20D_amidohydro. 1 hit.
    SUPFAMiSSF55031. SSF55031. 1 hit.
    TIGRFAMsiTIGR01891. amidohydrolases. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P37112-1 [UniParc]FASTAAdd to Basket

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    MTKEEIKRLV DEVKTDVIAW RRHLHAHPEL SFQEEKTAQF VYETLQSFGH    50
    LELSRPTKTS VMARLIGQQP GRVVAIRADM DALPIQEENT FEFASKNPGV 100
    MHACGHDGHT AMLLGTAKIF SQLRDDIRGE IRFLFQHAEE LFPGGAEEMV 150
    QAGVMDGVDV VIGTHLWSPL ERGKIGIVYG PMMAAPDRFF IRIIGKGGHG 200
    AMPHQTIDAI AIGAQVVTNL QHIVSRYVDP LEPLVLSVTQ FVAGTAHNVL 250
    PGEVEIQGTV RTFDETLRRT VPQWMERIVK GITEAHGASY EFRFDYGYRP 300
    VINYDEGDPR HGGNGVRAVR RRGSGPLETE HGRRRFLRLF AKSARQLFLR 350
    RRGQCRKRHR LPAPPPALYD 370
    Length:370
    Mass (Da):41,676
    Last modified:October 1, 1994 - v1
    Checksum:i479AF1559DB527FB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74289 Genomic DNA. Translation: CAA52342.1.
    Y08753 Genomic DNA. Translation: CAA70000.1.
    PIRiI40358.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74289 Genomic DNA. Translation: CAA52342.1 .
    Y08753 Genomic DNA. Translation: CAA70000.1 .
    PIRi I40358.

    3D structure databases

    ProteinModelPortali P37112.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.30.70.360. 1 hit.
    InterProi IPR017439. Amidohydrolase.
    IPR002933. Peptidase_M20.
    IPR011650. Peptidase_M20_dimer.
    [Graphical view ]
    Pfami PF07687. M20_dimer. 1 hit.
    PF01546. Peptidase_M20. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005962. Pept_M20D_amidohydro. 1 hit.
    SUPFAMi SSF55031. SSF55031. 1 hit.
    TIGRFAMsi TIGR01891. amidohydrolases. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Gene cloning, sequence analysis, purification, and characterization of a thermostable aminoacylase from Bacillus stearothermophilus."
      Sakanyan V., Desmarez L., Legrain C., Charlier D.R.M., Mett I., Kochikyan A., Savchenko A., Boyen A., Falmagne P., Pirard A., Glansdorff N.
      Appl. Environ. Microbiol. 59:3878-3888(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10, CHARACTERIZATION.
      Strain: NCIMB 8224 / CCM 2186 / VKM B-718.
    2. "Two amino acid amidohydrolase genes encoding L-stereospecific carbamoylase and aminoacylase are organized in a common operon in Bacillus stearothermophilus."
      Batisse N., Weigel P., Lecocq M., Sakanyan V.
      Appl. Environ. Microbiol. 63:763-766(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: NCIMB 8224 / CCM 2186 / VKM B-718.

    Entry informationi

    Entry nameiAMAA_GEOSE
    AccessioniPrimary (citable) accession number: P37112
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3