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P37112

- AMAA_GEOSE

UniProt

P37112 - AMAA_GEOSE

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Protein

N-acyl-L-amino acid amidohydrolase

Gene
amaA, ama
Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes most efficiently N-acetyl derivatives of aromatic amino acids but is also active on other amino acids. L-stereospecific.

Catalytic activityi

An N-acyl-aliphatic-L-amino acid + H2O = an aliphatic L-amino acid + a carboxylate.
An N-acetyl-L-cysteine-S-conjugate + H2O = an L-cysteine-S-conjugate + acetate.

Cofactori

Cobalt.

GO - Molecular functioni

  1. aminoacylase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Cobalt

Names & Taxonomyi

Protein namesi
Recommended name:
N-acyl-L-amino acid amidohydrolase (EC:3.5.1.14)
Short name:
L-aminoacylase
Gene namesi
Name:amaA
Synonyms:ama
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 370370N-acyl-L-amino acid amidohydrolasePRO_0000061949Add
BLAST

Interactioni

Subunit structurei

Homotetramer.

Structurei

3D structure databases

ProteinModelPortaliP37112.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M20 family.

Family and domain databases

Gene3Di3.30.70.360. 1 hit.
InterProiIPR017439. Amidohydrolase.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
[Graphical view]
PfamiPF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
[Graphical view]
PIRSFiPIRSF005962. Pept_M20D_amidohydro. 1 hit.
SUPFAMiSSF55031. SSF55031. 1 hit.
TIGRFAMsiTIGR01891. amidohydrolases. 1 hit.

Sequencei

Sequence statusi: Complete.

P37112-1 [UniParc]FASTAAdd to Basket

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MTKEEIKRLV DEVKTDVIAW RRHLHAHPEL SFQEEKTAQF VYETLQSFGH    50
LELSRPTKTS VMARLIGQQP GRVVAIRADM DALPIQEENT FEFASKNPGV 100
MHACGHDGHT AMLLGTAKIF SQLRDDIRGE IRFLFQHAEE LFPGGAEEMV 150
QAGVMDGVDV VIGTHLWSPL ERGKIGIVYG PMMAAPDRFF IRIIGKGGHG 200
AMPHQTIDAI AIGAQVVTNL QHIVSRYVDP LEPLVLSVTQ FVAGTAHNVL 250
PGEVEIQGTV RTFDETLRRT VPQWMERIVK GITEAHGASY EFRFDYGYRP 300
VINYDEGDPR HGGNGVRAVR RRGSGPLETE HGRRRFLRLF AKSARQLFLR 350
RRGQCRKRHR LPAPPPALYD 370
Length:370
Mass (Da):41,676
Last modified:October 1, 1994 - v1
Checksum:i479AF1559DB527FB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X74289 Genomic DNA. Translation: CAA52342.1.
Y08753 Genomic DNA. Translation: CAA70000.1.
PIRiI40358.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X74289 Genomic DNA. Translation: CAA52342.1 .
Y08753 Genomic DNA. Translation: CAA70000.1 .
PIRi I40358.

3D structure databases

ProteinModelPortali P37112.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.30.70.360. 1 hit.
InterProi IPR017439. Amidohydrolase.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
[Graphical view ]
Pfami PF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
[Graphical view ]
PIRSFi PIRSF005962. Pept_M20D_amidohydro. 1 hit.
SUPFAMi SSF55031. SSF55031. 1 hit.
TIGRFAMsi TIGR01891. amidohydrolases. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Gene cloning, sequence analysis, purification, and characterization of a thermostable aminoacylase from Bacillus stearothermophilus."
    Sakanyan V., Desmarez L., Legrain C., Charlier D.R.M., Mett I., Kochikyan A., Savchenko A., Boyen A., Falmagne P., Pirard A., Glansdorff N.
    Appl. Environ. Microbiol. 59:3878-3888(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10, CHARACTERIZATION.
    Strain: NCIMB 8224 / CCM 2186 / VKM B-718.
  2. "Two amino acid amidohydrolase genes encoding L-stereospecific carbamoylase and aminoacylase are organized in a common operon in Bacillus stearothermophilus."
    Batisse N., Weigel P., Lecocq M., Sakanyan V.
    Appl. Environ. Microbiol. 63:763-766(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: NCIMB 8224 / CCM 2186 / VKM B-718.

Entry informationi

Entry nameiAMAA_GEOSE
AccessioniPrimary (citable) accession number: P37112
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: February 19, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3