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P37111

- ACY1_PIG

UniProt

P37111 - ACY1_PIG

Protein

Aminoacylase-1

Gene

ACY1

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate).

    Catalytic activityi

    An N-acyl-aliphatic-L-amino acid + H2O = an aliphatic L-amino acid + a carboxylate.
    An N-acetyl-L-cysteine-S-conjugate + H2O = an L-cysteine-S-conjugate + acetate.

    Cofactori

    Binds 2 zinc ions per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi80 – 801Zinc 1By similarity
    Active sitei82 – 821By similarity
    Metal bindingi113 – 1131Zinc 1By similarity
    Metal bindingi113 – 1131Zinc 2By similarity
    Active sitei147 – 1471Proton acceptorBy similarity
    Metal bindingi148 – 1481Zinc 2By similarity
    Metal bindingi175 – 1751Zinc 1By similarity
    Metal bindingi372 – 3721Zinc 2By similarity

    GO - Molecular functioni

    1. aminoacylase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW
    3. metallopeptidase activity Source: InterPro

    GO - Biological processi

    1. cellular amino acid metabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BRENDAi3.5.1.14. 6170.
    SABIO-RKP37111.

    Protein family/group databases

    MEROPSiM20.973.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aminoacylase-1 (EC:3.5.1.14)
    Short name:
    ACY-1
    Alternative name(s):
    N-acyl-L-amino-acid amidohydrolase
    Gene namesi
    Name:ACY1
    OrganismiSus scrofa (Pig)
    Taxonomic identifieri9823 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
    ProteomesiUP000008227: Chromosome 13

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 407406Aminoacylase-1PRO_0000185237Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine

    Keywords - PTMi

    Acetylation

    Interactioni

    Subunit structurei

    Homodimer. Interacts with SPHK1 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP37111.
    SMRiP37111. Positions 7-198, 320-407.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M20A family.Curated

    Phylogenomic databases

    GeneTreeiENSGT00730000111049.
    HOVERGENiHBG000982.
    KOiK14677.
    OMAiKGMELFV.
    OrthoDBiEOG7N8ZVZ.
    TreeFamiTF313693.

    Family and domain databases

    Gene3Di3.30.70.360. 1 hit.
    InterProiIPR001261. ArgE/DapE_CS.
    IPR010159. N-acyl_aa_amidohydrolase.
    IPR002933. Peptidase_M20.
    IPR011650. Peptidase_M20_dimer.
    [Graphical view]
    PfamiPF07687. M20_dimer. 1 hit.
    PF01546. Peptidase_M20. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036696. ACY-1. 1 hit.
    SUPFAMiSSF55031. SSF55031. 1 hit.
    TIGRFAMsiTIGR01880. Ac-peptdase-euk. 1 hit.
    PROSITEiPS00758. ARGE_DAPE_CPG2_1. 1 hit.
    PS00759. ARGE_DAPE_CPG2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P37111-1 [UniParc]FASTAAdd to Basket

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    MASKGREGEH PSVTLFRQYL RIRTVQPEPD YGAAVAFLEE RARQLGLGCQ    50
    KVEVVPGHVV TVLTWPGTNP TLSSILLNSH TDVVPVFKEH WSHDPFEGFK 100
    DADGYIYGRG AQDMKCVSIQ YLEAVRRLKV EGHHFPRTIH MTFVPDEEVG 150
    GHQGMELFVK RPEFQALRAG FALDEGLASP TDAFTVFYSE RSPWWLRVTS 200
    TGKPGHGSRF IEDTAAEKLH KVINSILAFR EKEKQRLQSN QLKPGAVTSV 250
    NLTMLEGGVA YNVVPATMSA CFDFRVAPDV DLKAFEEQLQ SWCQAAGEGV 300
    TFEFVQKWME TQVTSTDDSD PWWAAFSGVF KDMKLALELE ICPASTDARY 350
    IRAAGVPALG FSPMNHTPVL LHDHDERLHE AVFLRGVDIY TQLLSALASV 400
    PALPSES 407
    Length:407
    Mass (Da):45,347
    Last modified:January 23, 2007 - v2
    Checksum:iFCB88982ADBFF3D4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti395 – 3951Missing in CAA48565. (PubMed:1292507)Curated
    Sequence conflicti398 – 3981A → T in CAA48565. (PubMed:1292507)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13514 mRNA. Translation: BAA02731.1.
    X68564 mRNA. Translation: CAA48565.1.
    AB017196 Genomic DNA. Translation: BAA76403.1.
    PIRiJN0584.
    RefSeqiNP_999061.1. NM_213896.1.
    UniGeneiSsc.14528.

    Genome annotation databases

    EnsembliENSSSCT00000027267; ENSSSCP00000025679; ENSSSCG00000023325.
    GeneIDi396930.
    KEGGissc:396930.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13514 mRNA. Translation: BAA02731.1 .
    X68564 mRNA. Translation: CAA48565.1 .
    AB017196 Genomic DNA. Translation: BAA76403.1 .
    PIRi JN0584.
    RefSeqi NP_999061.1. NM_213896.1.
    UniGenei Ssc.14528.

    3D structure databases

    ProteinModelPortali P37111.
    SMRi P37111. Positions 7-198, 320-407.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi M20.973.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSSSCT00000027267 ; ENSSSCP00000025679 ; ENSSSCG00000023325 .
    GeneIDi 396930.
    KEGGi ssc:396930.

    Organism-specific databases

    CTDi 95.

    Phylogenomic databases

    GeneTreei ENSGT00730000111049.
    HOVERGENi HBG000982.
    KOi K14677.
    OMAi KGMELFV.
    OrthoDBi EOG7N8ZVZ.
    TreeFami TF313693.

    Enzyme and pathway databases

    BRENDAi 3.5.1.14. 6170.
    SABIO-RK P37111.

    Family and domain databases

    Gene3Di 3.30.70.360. 1 hit.
    InterProi IPR001261. ArgE/DapE_CS.
    IPR010159. N-acyl_aa_amidohydrolase.
    IPR002933. Peptidase_M20.
    IPR011650. Peptidase_M20_dimer.
    [Graphical view ]
    Pfami PF07687. M20_dimer. 1 hit.
    PF01546. Peptidase_M20. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036696. ACY-1. 1 hit.
    SUPFAMi SSF55031. SSF55031. 1 hit.
    TIGRFAMsi TIGR01880. Ac-peptdase-euk. 1 hit.
    PROSITEi PS00758. ARGE_DAPE_CPG2_1. 1 hit.
    PS00759. ARGE_DAPE_CPG2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The primary structure of porcine aminoacylase 1 deduced from cDNA sequence."
      Mitta M., Ohnogi H., Yamamoto A., Kato I., Sakiyama F., Tsunasawa S.
      J. Biochem. 112:737-742(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Tissue: Kidney.
    2. "Cloning and sequence analyses of cDNAs encoding aminoacylase I from porcine kidney."
      Jakob M., Miller Y.E., Roehm K.H.
      Biol. Chem. Hoppe-Seyler 373:1227-1231(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Kidney.
    3. "Porcine cosmid clone containing the ACY-1 and rpL29/HIP genes."
      Sawazaki T., Hamasima N.
      Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiACY1_PIG
    AccessioniPrimary (citable) accession number: P37111
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 86 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3