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Protein

Aminoacylase-1

Gene

ACY1

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate).

Catalytic activityi

An N-acyl-aliphatic-L-amino acid + H2O = an aliphatic L-amino acid + a carboxylate.
An N-acetyl-L-cysteine-S-conjugate + H2O = an L-cysteine-S-conjugate + acetate.

Cofactori

Zn2+Note: Binds 2 Zn2+ ions per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi80 – 801Zinc 1By similarity
Active sitei82 – 821By similarity
Metal bindingi113 – 1131Zinc 1By similarity
Metal bindingi113 – 1131Zinc 2By similarity
Active sitei147 – 1471Proton acceptorBy similarity
Metal bindingi148 – 1481Zinc 2By similarity
Metal bindingi175 – 1751Zinc 1By similarity
Metal bindingi372 – 3721Zinc 2By similarity

GO - Molecular functioni

  1. aminoacylase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. metallopeptidase activity Source: InterPro

GO - Biological processi

  1. cellular amino acid metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.5.1.14. 6170.
ReactomeiREACT_261486. Aflatoxin activation and detoxification.
SABIO-RKP37111.

Protein family/group databases

MEROPSiM20.973.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminoacylase-1 (EC:3.5.1.14)
Short name:
ACY-1
Alternative name(s):
N-acyl-L-amino-acid amidohydrolase
Gene namesi
Name:ACY1
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Chromosome 13

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 407406Aminoacylase-1PRO_0000185237Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine

Keywords - PTMi

Acetylation

Interactioni

Subunit structurei

Homodimer. Interacts with SPHK1 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP37111.
SMRiP37111. Positions 7-198, 320-407.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M20A family.Curated

Phylogenomic databases

GeneTreeiENSGT00730000111049.
HOVERGENiHBG000982.
InParanoidiP37111.
KOiK14677.
OMAiKGMELFV.
OrthoDBiEOG7N8ZVZ.
TreeFamiTF313693.

Family and domain databases

Gene3Di3.30.70.360. 1 hit.
InterProiIPR001261. ArgE/DapE_CS.
IPR010159. N-acyl_aa_amidohydrolase.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
[Graphical view]
PfamiPF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
[Graphical view]
PIRSFiPIRSF036696. ACY-1. 1 hit.
SUPFAMiSSF55031. SSF55031. 1 hit.
TIGRFAMsiTIGR01880. Ac-peptdase-euk. 1 hit.
PROSITEiPS00758. ARGE_DAPE_CPG2_1. 1 hit.
PS00759. ARGE_DAPE_CPG2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37111-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASKGREGEH PSVTLFRQYL RIRTVQPEPD YGAAVAFLEE RARQLGLGCQ
60 70 80 90 100
KVEVVPGHVV TVLTWPGTNP TLSSILLNSH TDVVPVFKEH WSHDPFEGFK
110 120 130 140 150
DADGYIYGRG AQDMKCVSIQ YLEAVRRLKV EGHHFPRTIH MTFVPDEEVG
160 170 180 190 200
GHQGMELFVK RPEFQALRAG FALDEGLASP TDAFTVFYSE RSPWWLRVTS
210 220 230 240 250
TGKPGHGSRF IEDTAAEKLH KVINSILAFR EKEKQRLQSN QLKPGAVTSV
260 270 280 290 300
NLTMLEGGVA YNVVPATMSA CFDFRVAPDV DLKAFEEQLQ SWCQAAGEGV
310 320 330 340 350
TFEFVQKWME TQVTSTDDSD PWWAAFSGVF KDMKLALELE ICPASTDARY
360 370 380 390 400
IRAAGVPALG FSPMNHTPVL LHDHDERLHE AVFLRGVDIY TQLLSALASV

PALPSES
Length:407
Mass (Da):45,347
Last modified:January 23, 2007 - v2
Checksum:iFCB88982ADBFF3D4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti395 – 3951Missing in CAA48565. (PubMed:1292507)Curated
Sequence conflicti398 – 3981A → T in CAA48565. (PubMed:1292507)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13514 mRNA. Translation: BAA02731.1.
X68564 mRNA. Translation: CAA48565.1.
AB017196 Genomic DNA. Translation: BAA76403.1.
PIRiJN0584.
RefSeqiNP_999061.1. NM_213896.1.
UniGeneiSsc.14528.

Genome annotation databases

EnsembliENSSSCT00000027267; ENSSSCP00000025679; ENSSSCG00000023325.
GeneIDi396930.
KEGGissc:396930.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13514 mRNA. Translation: BAA02731.1.
X68564 mRNA. Translation: CAA48565.1.
AB017196 Genomic DNA. Translation: BAA76403.1.
PIRiJN0584.
RefSeqiNP_999061.1. NM_213896.1.
UniGeneiSsc.14528.

3D structure databases

ProteinModelPortaliP37111.
SMRiP37111. Positions 7-198, 320-407.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiM20.973.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000027267; ENSSSCP00000025679; ENSSSCG00000023325.
GeneIDi396930.
KEGGissc:396930.

Organism-specific databases

CTDi95.

Phylogenomic databases

GeneTreeiENSGT00730000111049.
HOVERGENiHBG000982.
InParanoidiP37111.
KOiK14677.
OMAiKGMELFV.
OrthoDBiEOG7N8ZVZ.
TreeFamiTF313693.

Enzyme and pathway databases

BRENDAi3.5.1.14. 6170.
ReactomeiREACT_261486. Aflatoxin activation and detoxification.
SABIO-RKP37111.

Family and domain databases

Gene3Di3.30.70.360. 1 hit.
InterProiIPR001261. ArgE/DapE_CS.
IPR010159. N-acyl_aa_amidohydrolase.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
[Graphical view]
PfamiPF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
[Graphical view]
PIRSFiPIRSF036696. ACY-1. 1 hit.
SUPFAMiSSF55031. SSF55031. 1 hit.
TIGRFAMsiTIGR01880. Ac-peptdase-euk. 1 hit.
PROSITEiPS00758. ARGE_DAPE_CPG2_1. 1 hit.
PS00759. ARGE_DAPE_CPG2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The primary structure of porcine aminoacylase 1 deduced from cDNA sequence."
    Mitta M., Ohnogi H., Yamamoto A., Kato I., Sakiyama F., Tsunasawa S.
    J. Biochem. 112:737-742(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Kidney.
  2. "Cloning and sequence analyses of cDNAs encoding aminoacylase I from porcine kidney."
    Jakob M., Miller Y.E., Roehm K.H.
    Biol. Chem. Hoppe-Seyler 373:1227-1231(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.
  3. "Porcine cosmid clone containing the ACY-1 and rpL29/HIP genes."
    Sawazaki T., Hamasima N.
    Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiACY1_PIG
AccessioniPrimary (citable) accession number: P37111
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: January 23, 2007
Last modified: January 7, 2015
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.