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Protein

Signal recognition particle 54 kDa protein, chloroplastic

Gene

FFC

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in cotranslational and post-translational sorting of thylakoid proteins. Binds GTP specifically. Activates the GTPase activity of CPFTSY when bound together. Required for light-harvesting chlorophyll a/b-binding protein (LHCP) integration into thylakoids.4 Publications

Miscellaneous

Unlike eukaryotic or prokaryotic signal recognition particle (SRP), the chloroplast SRP from higher plants lacks an SRP-RNA component. It targets both chloroplast-encoded and nucleus-encoded substrates to the thylakoid membrane, post-translationally for the nucleus-encoded proteins and co-translationally for the chloroplast-encoded proteins.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi183 – 190GTPBy similarity8
Nucleotide bindingi265 – 269GTPBy similarity5
Nucleotide bindingi323 – 326GTPBy similarity4

GO - Molecular functioni

  • 7S RNA binding Source: InterPro
  • GTPase activity Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • protein domain specific binding Source: CAFA

GO - Biological processi

  • protein heterotrimerization Source: CAFA
  • SRP-dependent cotranslational protein targeting to membrane Source: InterPro

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
LigandGTP-binding, Nucleotide-binding

Protein family/group databases

TCDBi3.A.5.1.2 the general secretory pathway (sec) family

Names & Taxonomyi

Protein namesi
Recommended name:
Signal recognition particle 54 kDa protein, chloroplastic
Short name:
54 chloroplast protein
Short name:
54CP
Short name:
SRP54
Short name:
cpSRP54
Alternative name(s):
FFC
Gene namesi
Name:FFC
Synonyms:CPSRP54
Ordered Locus Names:At5g03940
ORF Names:F8F6_150
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G03940
TAIRilocus:2150620 AT5G03940

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid, Signal recognition particle

Pathology & Biotechi

Disruption phenotypei

Pale green with delayed development.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi536R → G or K: Strongly reduced binding to CAO/cpSRP43. 1 Publication1
Mutagenesisi537R → G, Q, K or N: Loss of binding to CAO/cpSRP43. 1 Publication1
Mutagenesisi538 – 540Missing : No effect on binding to CAO/cpSRP43. 3
Mutagenesisi538K → M: No effect on binding to CAO/cpSRP43. 1 Publication1
Mutagenesisi539R → G or K: Reduced binding to CAO/cpSRP43. 1 Publication1
Mutagenesisi540K → M: No effect on binding to CAO/cpSRP43. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 75ChloroplastAdd BLAST75
ChainiPRO_000003323276 – 564Signal recognition particle 54 kDa protein, chloroplasticAdd BLAST489

Proteomic databases

PaxDbiP37107
PRIDEiP37107

PTM databases

iPTMnetiP37107

Expressioni

Tissue specificityi

Most abundant in green shoot tissue and lower levels seen in the roots and etiolated buds.1 Publication

Gene expression databases

ExpressionAtlasiP37107 baseline and differential
GenevisibleiP37107 AT

Interactioni

Subunit structurei

Component of the cpSRP complex, composed of a FFC/cpSRP54 monomer and a CAO/cpSRP43 dimer. Interacts (via C-terminus) with CAO/cpSRP43 and with CPFTSY. Interacts with the third transmembrane domain of LHCP. Interacts with the PSBA/D1 nascent chain, but only when it is attached to the 70S ribosome and if it is shorter than 189 amino acid residues.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CAOO2226521EBI-780642,EBI-780656

GO - Molecular functioni

  • protein domain specific binding Source: CAFA

Protein-protein interaction databases

BioGridi15553, 4 interactors
DIPiDIP-752N
IntActiP37107, 4 interactors
MINTiP37107
STRINGi3702.AT5G03940.1

Structurei

Secondary structure

1564
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi104 – 116Combined sources13
Helixi121 – 138Combined sources18
Helixi146 – 161Combined sources16
Beta strandi173 – 175Combined sources3
Beta strandi177 – 182Combined sources6
Helixi189 – 201Combined sources13
Turni202 – 204Combined sources3
Beta strandi207 – 212Combined sources6
Helixi219 – 230Combined sources12
Helixi243 – 256Combined sources14
Beta strandi260 – 266Combined sources7
Helixi274 – 287Combined sources14
Beta strandi290 – 297Combined sources8
Helixi304 – 314Combined sources11
Beta strandi318 – 323Combined sources6
Helixi333 – 341Combined sources9
Beta strandi345 – 349Combined sources5
Beta strandi351 – 353Combined sources3
Beta strandi357 – 359Combined sources3
Helixi362 – 369Combined sources8
Beta strandi534 – 538Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HUGNMR-B530-543[»]
3UI2X-ray3.18B528-540[»]
5L3RX-ray2.50A/C77-371[»]
ProteinModelPortaliP37107
SMRiP37107
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37107

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni76 – 370G-domainAdd BLAST295
Regioni371 – 564M-domainAdd BLAST194

Domaini

The M-domain stimulates the interaction between CPFTSY and the chloroplast signal recognition particle (cpSRP).
The C-terminal residues (539-564) are required for interaction with CAO/cpSRP43.

Sequence similaritiesi

Belongs to the GTP-binding SRP family. SRP54 subfamily.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0780 Eukaryota
COG0541 LUCA
HOGENOMiHOG000036164
InParanoidiP37107
KOiK03106
OMAiIGMMPGM
OrthoDBiEOG0936062N
PhylomeDBiP37107

Family and domain databases

Gene3Di1.10.260.30, 1 hit
HAMAPiMF_00306 SRP54, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR027417 P-loop_NTPase
IPR036891 Signal_recog_part_SRP54_M_sf
IPR013822 Signal_recog_particl_SRP54_hlx
IPR004125 Signal_recog_particle_SRP54_M
IPR022941 SRP54
IPR000897 SRP54_GTPase_dom
IPR004780 SRP_Ffh
PANTHERiPTHR11564 PTHR11564, 1 hit
PfamiView protein in Pfam
PF00448 SRP54, 1 hit
PF02881 SRP54_N, 1 hit
PF02978 SRP_SPB, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00962 SRP54, 1 hit
SM00963 SRP54_N, 1 hit
SUPFAMiSSF47446 SSF47446, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00959 ffh, 1 hit
PROSITEiView protein in PROSITE
PS00300 SRP54, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37107-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEALQFSSVN RVPCTLSCTG NRRIKAAFSS AFTGGTINSA SLSSSRNLST
60 70 80 90 100
REIWSWVKSK TVVGHGRYRR SQVRAEMFGQ LTGGLEAAWS KLKGEEVLTK
110 120 130 140 150
DNIAEPMRDI RRALLEADVS LPVVRRFVQS VSDQAVGMGV IRGVKPDQQL
160 170 180 190 200
VKIVHDELVK LMGGEVSELQ FAKSGPTVIL LAGLQGVGKT TVCAKLACYL
210 220 230 240 250
KKQGKSCMLI AGDVYRPAAI DQLVILGEQV GVPVYTAGTD VKPADIAKQG
260 270 280 290 300
LKEAKKNNVD VVIMDTAGRL QIDKGMMDEL KDVKKFLNPT EVLLVVDAMT
310 320 330 340 350
GQEAAALVTT FNVEIGITGA ILTKLDGDSR GGAALSVKEV SGKPIKLVGR
360 370 380 390 400
GERMEDLEPF YPDRMAGRIL GMGDVLSFVE KATEVMRQED AEDLQKKIMS
410 420 430 440 450
AKFDFNDFLK QTRAVAKMGS MTRVLGMIPG MGKVSPAQIR EAEKNLLVME
460 470 480 490 500
AMIEVMTPEE RERPELLAES PERRKRIAKD SGKTEQQVSA LVAQIFQMRV
510 520 530 540 550
KMKNLMGVME GGSIPALSGL EDALKAEQKA PPGTARRKRK ADSRKKFVES
560
ASSKPGPRGF GSGN
Length:564
Mass (Da):61,232
Last modified:October 1, 1994 - v1
Checksum:i423F7285FB9063E4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti76E → V in AAC64139 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z21970 mRNA Translation: CAA79981.1
AF092168 Genomic DNA Translation: AAC64139.1
AL162873 Genomic DNA Translation: CAB85514.1
CP002688 Genomic DNA Translation: AED90674.1
AY050999 mRNA Translation: AAK93676.1
BT000976 mRNA Translation: AAN41376.1
PIRiS36637
RefSeqiNP_196014.1, NM_120476.5
UniGeneiAt.435

Genome annotation databases

EnsemblPlantsiAT5G03940.1; AT5G03940.1; AT5G03940
GeneIDi830273
GrameneiAT5G03940.1; AT5G03940.1; AT5G03940
KEGGiath:AT5G03940

Similar proteinsi

Entry informationi

Entry nameiSR54C_ARATH
AccessioniPrimary (citable) accession number: P37107
Secondary accession number(s): O82570
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: April 25, 2018
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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