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Protein

Signal recognition particle protein

Gene

ffh

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. Interaction with FtsY leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components (Probable).1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi108 – 1158GTPUniRule annotation
Nucleotide bindingi191 – 1955GTPUniRule annotation
Nucleotide bindingi249 – 2524GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Ligandi

GTP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciBSUB:BSU15980-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal recognition particle proteinUniRule annotation
Alternative name(s):
Fifty-four homologUniRule annotation
Gene namesi
Name:ffhUniRule annotation
Ordered Locus Names:BSU15980
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication

  • Note: The SRP-RNC complex is targeted to the cytoplasmic membrane.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Signal recognition particle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 446446Signal recognition particle proteinPRO_0000101149Add
BLAST

Proteomic databases

PaxDbiP37105.

Interactioni

Subunit structurei

Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY (By similarity). Interacts with a small cytoplasmic RNA (sc-RNA).UniRule annotation1 Publication

Protein-protein interaction databases

IntActiP37105. 1 interaction.
MINTiMINT-8367134.
STRINGi224308.Bsubs1_010100008811.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4UE4electron microscopy7.00C330-431[»]
ProteinModelPortaliP37105.
SMRiP37105. Positions 3-431.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.UniRule annotation

Sequence similaritiesi

Belongs to the GTP-binding SRP family. SRP54 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0541. LUCA.
HOGENOMiHOG000036164.
InParanoidiP37105.
KOiK03106.
OMAiMLPGMGQ.
OrthoDBiEOG62K1ZH.
PhylomeDBiP37105.

Family and domain databases

Gene3Di1.10.260.30. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00306. SRP54.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR000897. SRP54_GTPase_dom.
IPR004780. SRP_Ffh.
[Graphical view]
PANTHERiPTHR11564:SF7. PTHR11564:SF7. 1 hit.
PfamiPF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMiSSF47446. SSF47446. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00959. ffh. 1 hit.
PROSITEiPS00300. SRP54. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37105-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFEGLADRL QQTISKIRGK GKVSEQDVKE MMREVRLALL EADVNFKVVK
60 70 80 90 100
DFVKKVSERA VGQDVMKSLT PGQQVIKVVQ EELTELMGGE ESKIAVAKRP
110 120 130 140 150
PTVIMMVGLQ GAGKTTTSGK LANLLRKKHN RKPMLVAADI YRPAAIKQLE
160 170 180 190 200
TLGKQLDMPV FSLGDQVSPV EIAKQAIEKA KEEHYDYVIL DTAGRLHIDH
210 220 230 240 250
ELMDELTNVK EIANPEEIFL VVDSMTGQDA VNVAKSFNEQ LGLTGVVLTK
260 270 280 290 300
LDGDTRGGAA LSIRAVTNTP IKFAGLGEKL DALEPFHPER MASRILGMGD
310 320 330 340 350
VLTLIEKAQA SVDEDKAKEL EQKMRTMSFT LDDFLEQLGQ VRNMGPLDEL
360 370 380 390 400
LQMMPGAGKM KGLKNIQVDE KQLNHVEAII KSMTVLEKEQ PDIINASRRK
410 420 430 440
RIAKGSGTSV QEVNRLLKQF DEMKKMMKQM TNMSKGKKKG FKLPFM
Length:446
Mass (Da):49,541
Last modified:October 1, 1994 - v1
Checksum:iD71943B23374633D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14356 Genomic DNA. Translation: BAA21691.1.
AL009126 Genomic DNA. Translation: CAB13471.1.
PIRiB47154.
RefSeqiNP_389480.1. NC_000964.3.
WP_003232020.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13471; CAB13471; BSU15980.
GeneIDi937104.
KEGGibsu:BSU15980.
PATRICi18975001. VBIBacSub10457_1692.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14356 Genomic DNA. Translation: BAA21691.1.
AL009126 Genomic DNA. Translation: CAB13471.1.
PIRiB47154.
RefSeqiNP_389480.1. NC_000964.3.
WP_003232020.1. NZ_JNCM01000035.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4UE4electron microscopy7.00C330-431[»]
ProteinModelPortaliP37105.
SMRiP37105. Positions 3-431.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP37105. 1 interaction.
MINTiMINT-8367134.
STRINGi224308.Bsubs1_010100008811.

Proteomic databases

PaxDbiP37105.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13471; CAB13471; BSU15980.
GeneIDi937104.
KEGGibsu:BSU15980.
PATRICi18975001. VBIBacSub10457_1692.

Phylogenomic databases

eggNOGiCOG0541. LUCA.
HOGENOMiHOG000036164.
InParanoidiP37105.
KOiK03106.
OMAiMLPGMGQ.
OrthoDBiEOG62K1ZH.
PhylomeDBiP37105.

Enzyme and pathway databases

BioCyciBSUB:BSU15980-MONOMER.

Family and domain databases

Gene3Di1.10.260.30. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00306. SRP54.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR000897. SRP54_GTPase_dom.
IPR004780. SRP_Ffh.
[Graphical view]
PANTHERiPTHR11564:SF7. PTHR11564:SF7. 1 hit.
PfamiPF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMiSSF47446. SSF47446. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00959. ffh. 1 hit.
PROSITEiPS00300. SRP54. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a Bacillus subtilis gene encoding a homolog of the 54-kilodalton subunit of mammalian signal recognition particle and Escherichia coli Ffh."
    Honda K., Nakamura K., Nishiguchi M., Yamane K.
    J. Bacteriol. 175:4885-4894(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "The Bacillus subtilis SRP54 homologue, Ffh, has an intrinsic GTPase activity and forms a ribonucleoprotein complex with small cytoplasmic RNA in vivo."
    Nakamura K., Nishiguchi M., Honda K., Yamane K.
    Biochem. Biophys. Res. Commun. 199:1394-1399(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, GTPASE ACTIVITY, INTERACTION WITH RNA, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiSRP54_BACSU
AccessioniPrimary (citable) accession number: P37105
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: June 8, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.