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Protein

Signal recognition particle protein

Gene

ffh

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. Interaction with FtsY leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components (Probable).1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi108 – 115GTPUniRule annotation8
Nucleotide bindingi191 – 195GTPUniRule annotation5
Nucleotide bindingi249 – 252GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU15980-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Signal recognition particle proteinUniRule annotation
Alternative name(s):
Fifty-four homologUniRule annotation
Gene namesi
Name:ffhUniRule annotation
Ordered Locus Names:BSU15980
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication
  • Note: The SRP-RNC complex is targeted to the cytoplasmic membrane.

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Signal recognition particle

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001011491 – 446Signal recognition particle proteinAdd BLAST446

Proteomic databases

PaxDbiP37105
PRIDEiP37105

Interactioni

Subunit structurei

Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY (By similarity). Interacts with a small cytoplasmic RNA (sc-RNA).UniRule annotation1 Publication

Protein-protein interaction databases

IntActiP37105, 1 interactor
MINTiP37105
STRINGi224308.Bsubs1_010100008811

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4UE4electron microscopy7.00C330-431[»]
ProteinModelPortaliP37105
SMRiP37105
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.UniRule annotation

Sequence similaritiesi

Belongs to the GTP-binding SRP family. SRP54 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0541 LUCA
HOGENOMiHOG000036164
InParanoidiP37105
KOiK03106
OMAiDTAGRHK
PhylomeDBiP37105

Family and domain databases

Gene3Di1.10.260.30, 1 hit
HAMAPiMF_00306 SRP54, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR027417 P-loop_NTPase
IPR036891 Signal_recog_part_SRP54_M_sf
IPR013822 Signal_recog_particl_SRP54_hlx
IPR004125 Signal_recog_particle_SRP54_M
IPR022941 SRP54
IPR000897 SRP54_GTPase_dom
IPR004780 SRP_Ffh
PANTHERiPTHR11564 PTHR11564, 1 hit
PTHR11564:SF7 PTHR11564:SF7, 1 hit
PfamiView protein in Pfam
PF00448 SRP54, 1 hit
PF02881 SRP54_N, 1 hit
PF02978 SRP_SPB, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00962 SRP54, 1 hit
SM00963 SRP54_N, 1 hit
SUPFAMiSSF47446 SSF47446, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00959 ffh, 1 hit
PROSITEiView protein in PROSITE
PS00300 SRP54, 1 hit

Sequencei

Sequence statusi: Complete.

P37105-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFEGLADRL QQTISKIRGK GKVSEQDVKE MMREVRLALL EADVNFKVVK
60 70 80 90 100
DFVKKVSERA VGQDVMKSLT PGQQVIKVVQ EELTELMGGE ESKIAVAKRP
110 120 130 140 150
PTVIMMVGLQ GAGKTTTSGK LANLLRKKHN RKPMLVAADI YRPAAIKQLE
160 170 180 190 200
TLGKQLDMPV FSLGDQVSPV EIAKQAIEKA KEEHYDYVIL DTAGRLHIDH
210 220 230 240 250
ELMDELTNVK EIANPEEIFL VVDSMTGQDA VNVAKSFNEQ LGLTGVVLTK
260 270 280 290 300
LDGDTRGGAA LSIRAVTNTP IKFAGLGEKL DALEPFHPER MASRILGMGD
310 320 330 340 350
VLTLIEKAQA SVDEDKAKEL EQKMRTMSFT LDDFLEQLGQ VRNMGPLDEL
360 370 380 390 400
LQMMPGAGKM KGLKNIQVDE KQLNHVEAII KSMTVLEKEQ PDIINASRRK
410 420 430 440
RIAKGSGTSV QEVNRLLKQF DEMKKMMKQM TNMSKGKKKG FKLPFM
Length:446
Mass (Da):49,541
Last modified:October 1, 1994 - v1
Checksum:iD71943B23374633D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14356 Genomic DNA Translation: BAA21691.1
AL009126 Genomic DNA Translation: CAB13471.1
PIRiB47154
RefSeqiNP_389480.1, NC_000964.3
WP_003232020.1, NZ_JNCM01000035.1

Genome annotation databases

EnsemblBacteriaiCAB13471; CAB13471; BSU15980
GeneIDi937104
KEGGibsu:BSU15980
PATRICifig|224308.179.peg.1738

Entry informationi

Entry nameiSRP54_BACSU
AccessioniPrimary (citable) accession number: P37105
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: March 28, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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