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Protein

Peptidase B

Gene

pepB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probably plays an important role in intracellular peptide degradation (PubMed:20067529).UniRule annotation1 Publication

Catalytic activityi

Release of an N-terminal amino acid, Xaa, from a peptide or arylamide. Xaa is preferably Glu or Asp but may be other amino acids, including Leu, Met, His, Cys and Gln.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 2 manganese ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi195Manganese 2UniRule annotation1
Metal bindingi200Manganese 1UniRule annotation1
Metal bindingi200Manganese 2UniRule annotation1
Active sitei207UniRule annotation1
Metal bindingi218Manganese 2UniRule annotation1
Metal bindingi277Manganese 1UniRule annotation1
Metal bindingi279Manganese 1UniRule annotation1
Metal bindingi279Manganese 2UniRule annotation1
Active sitei281UniRule annotation1

GO - Molecular functioni

  • aminopeptidase activity Source: EcoCyc
  • identical protein binding Source: EcoCyc
  • manganese ion binding Source: InterPro
  • metalloexopeptidase activity Source: InterPro
  • transcription regulatory region DNA binding Source: InterPro

GO - Biological processi

  • peptide catabolic process Source: EcoliWiki
  • proteolysis Source: EcoCyc

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Protease
LigandManganese, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12310-MONOMER
MetaCyc:EG12310-MONOMER
SABIO-RKiP37095

Protein family/group databases

MEROPSiM17.004

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidase BUniRule annotation (EC:3.4.11.23UniRule annotation)
Alternative name(s):
Aminopeptidase BUniRule annotation
Gene namesi
Name:pepBUniRule annotation
Synonyms:yfhI
Ordered Locus Names:b2523, JW2507
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12310 pepB

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

A quadruple peptidase disruption (pepA, pepB, pepD and pepN) does not grow in M9 minimal medium, grows better when supplemented with casamino acids (PubMed:20067529).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001658331 – 427Peptidase BAdd BLAST427

Proteomic databases

EPDiP37095
PaxDbiP37095
PRIDEiP37095

Interactioni

Subunit structurei

Homohexamer.UniRule annotation

Binary interactionsi

WithEntry#Exp.IntActNotes
pepEP0A7C62EBI-549539,EBI-555623

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4261583, 32 interactors
DIPiDIP-10455N
IntActiP37095, 16 interactors
STRINGi316385.ECDH10B_2690

Structurei

3D structure databases

ProteinModelPortaliP37095
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M17 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105BZ6 Bacteria
COG0260 LUCA
HOGENOMiHOG000243130
InParanoidiP37095
KOiK07751
OMAiEKSWAFW
PhylomeDBiP37095

Family and domain databases

CDDicd00433 Peptidase_M17, 1 hit
HAMAPiMF_00504 Aminopeptidase_M17, 1 hit
InterProiView protein in InterPro
IPR001346 Interferon_reg_fact_DNA-bd_dom
IPR011356 Leucine_aapep/pepB
IPR008330 Pept_M17_PepB
IPR000819 Peptidase_M17_C
PANTHERiPTHR11963 PTHR11963, 1 hit
PTHR11963:SF20 PTHR11963:SF20, 1 hit
PfamiView protein in Pfam
PF12404 DUF3663, 1 hit
PF00883 Peptidase_M17, 1 hit
PIRSFiPIRSF036388 Ctsl_amnpptdse_B, 1 hit
PRINTSiPR00481 LAMNOPPTDASE
PROSITEiView protein in PROSITE
PS00631 CYTOSOL_AP, 1 hit

Sequencei

Sequence statusi: Complete.

P37095-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEAMKITLS TQPADARWGE KATYSINNDG ITLHLNGADD LGLIQRAARK
60 70 80 90 100
IDGLGIKHVQ LSGEGWDADR CWAFWQGYKA PKGTRKVVWP DLDDAQRQEL
110 120 130 140 150
DNRLMIIDWV RDTINAPAEE LGPSQLAQRA VDLISNVAGD RVTYRITKGE
160 170 180 190 200
DLREQGYMGL HTVGRGSERS PVLLALDYNP TGDKEAPVYA CLVGKGITFD
210 220 230 240 250
SGGYSIKQTA FMDSMKSDMG GAATVTGALA FAITRGLNKR VKLFLCCADN
260 270 280 290 300
LISGNAFKLG DIITYRNGKK VEVMNTDAEG RLVLADGLID ASAQKPEMII
310 320 330 340 350
DAATLTGAAK TALGNDYHAL FSFDDALAGR LLASAAQENE PFWRLPLAEF
360 370 380 390 400
HRSQLPSNFA ELNNTGSAAY PAGASTAAGF LSHFVENYQQ GWLHIDCSAT
410 420
YRKAPVEQWS AGATGLGVRT IANLLTA
Length:427
Mass (Da):46,180
Last modified:November 1, 1997 - v2
Checksum:i8299E7D440F5732E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti41L → P in U01827 (PubMed:8300516).Curated1
Sequence conflicti155Q → P in BAA12689 (Ref. 1) Curated1
Sequence conflicti375 – 427STAAG…NLLTA → TRRRASCRTLLRTISKAGCI STARRLTVKRRLNSGLRALR DLVCAR in BAA12689 (Ref. 1) CuratedAdd BLAST53

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84499 Genomic DNA Translation: BAA12689.1
U00096 Genomic DNA Translation: AAC75576.2
AP009048 Genomic DNA Translation: BAA16413.1
U01827 Unassigned DNA No translation available.
PIRiB65029
RefSeqiNP_417018.4, NC_000913.3
WP_000133592.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75576; AAC75576; b2523
BAA16413; BAA16413; BAA16413
GeneIDi948766
KEGGiecj:JW2507
eco:b2523
PATRICifig|1411691.4.peg.4211

Similar proteinsi

Entry informationi

Entry nameiPEPB_ECOLI
AccessioniPrimary (citable) accession number: P37095
Secondary accession number(s): P76580
, P76989, P76991, Q47131
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1997
Last modified: April 25, 2018
This is version 152 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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