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Protein

Peptidase B

Gene

pepB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probably plays an important role in intracellular peptide degradation (PubMed:20067529).UniRule annotation1 Publication

Catalytic activityi

Release of an N-terminal amino acid, Xaa, from a peptide or arylamide. Xaa is preferably Glu or Asp but may be other amino acids, including Leu, Met, His, Cys and Gln.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 2 manganese ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi195 – 1951Manganese 2UniRule annotation
Metal bindingi200 – 2001Manganese 1UniRule annotation
Metal bindingi200 – 2001Manganese 2UniRule annotation
Active sitei207 – 2071UniRule annotation
Metal bindingi218 – 2181Manganese 2UniRule annotation
Metal bindingi277 – 2771Manganese 1UniRule annotation
Metal bindingi279 – 2791Manganese 1UniRule annotation
Metal bindingi279 – 2791Manganese 2UniRule annotation
Active sitei281 – 2811UniRule annotation

GO - Molecular functioni

  • aminopeptidase activity Source: EcoCyc
  • identical protein binding Source: EcoCyc
  • manganese ion binding Source: UniProtKB-HAMAP
  • metalloexopeptidase activity Source: InterPro

GO - Biological processi

  • peptide catabolic process Source: EcoliWiki
  • proteolysis Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12310-MONOMER.
ECOL316407:JW2507-MONOMER.
MetaCyc:EG12310-MONOMER.
SABIO-RKP37095.

Protein family/group databases

MEROPSiM17.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidase BUniRule annotation (EC:3.4.11.23UniRule annotation)
Alternative name(s):
Aminopeptidase BUniRule annotation
Gene namesi
Name:pepBUniRule annotation
Synonyms:yfhI
Ordered Locus Names:b2523, JW2507
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12310. pepB.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cytosol Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

A quadruple peptidase disruption (pepA, pepB, pepD and pepN) does not grow in M9 minimal medium, grows better when supplemented with casamino acids (PubMed:20067529).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 427427Peptidase BPRO_0000165833Add
BLAST

Proteomic databases

EPDiP37095.
PaxDbiP37095.
PRIDEiP37095.

Interactioni

Subunit structurei

Homohexamer.UniRule annotation

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4261583. 5 interactions.
DIPiDIP-10455N.
IntActiP37095. 16 interactions.
MINTiMINT-1236207.
STRINGi511145.b2523.

Structurei

3D structure databases

ProteinModelPortaliP37095.
SMRiP37095. Positions 78-425.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M17 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105BZ6. Bacteria.
COG0260. LUCA.
HOGENOMiHOG000243130.
InParanoidiP37095.
KOiK07751.
OMAiLARIECG.
PhylomeDBiP37095.

Family and domain databases

CDDicd00433. Peptidase_M17. 1 hit.
HAMAPiMF_00504. Aminopeptidase_M17. 1 hit.
InterProiIPR011356. Leucine_aapep/pepB.
IPR008330. Pept_M17_PepB.
IPR000819. Peptidase_M17_C.
[Graphical view]
PANTHERiPTHR11963. PTHR11963. 1 hit.
PfamiPF12404. DUF3663. 1 hit.
PF00883. Peptidase_M17. 1 hit.
[Graphical view]
PIRSFiPIRSF036388. Ctsl_amnpptdse_B. 1 hit.
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37095-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEAMKITLS TQPADARWGE KATYSINNDG ITLHLNGADD LGLIQRAARK
60 70 80 90 100
IDGLGIKHVQ LSGEGWDADR CWAFWQGYKA PKGTRKVVWP DLDDAQRQEL
110 120 130 140 150
DNRLMIIDWV RDTINAPAEE LGPSQLAQRA VDLISNVAGD RVTYRITKGE
160 170 180 190 200
DLREQGYMGL HTVGRGSERS PVLLALDYNP TGDKEAPVYA CLVGKGITFD
210 220 230 240 250
SGGYSIKQTA FMDSMKSDMG GAATVTGALA FAITRGLNKR VKLFLCCADN
260 270 280 290 300
LISGNAFKLG DIITYRNGKK VEVMNTDAEG RLVLADGLID ASAQKPEMII
310 320 330 340 350
DAATLTGAAK TALGNDYHAL FSFDDALAGR LLASAAQENE PFWRLPLAEF
360 370 380 390 400
HRSQLPSNFA ELNNTGSAAY PAGASTAAGF LSHFVENYQQ GWLHIDCSAT
410 420
YRKAPVEQWS AGATGLGVRT IANLLTA
Length:427
Mass (Da):46,180
Last modified:November 1, 1997 - v2
Checksum:i8299E7D440F5732E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti41 – 411L → P in U01827 (PubMed:8300516).Curated
Sequence conflicti155 – 1551Q → P in BAA12689 (Ref. 1) Curated
Sequence conflicti375 – 42753STAAG…NLLTA → TRRRASCRTLLRTISKAGCI STARRLTVKRRLNSGLRALR DLVCAR in BAA12689 (Ref. 1) CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84499 Genomic DNA. Translation: BAA12689.1.
U00096 Genomic DNA. Translation: AAC75576.2.
AP009048 Genomic DNA. Translation: BAA16413.1.
U01827 Unassigned DNA. No translation available.
PIRiB65029.
RefSeqiNP_417018.4. NC_000913.3.
WP_000133592.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75576; AAC75576; b2523.
BAA16413; BAA16413; BAA16413.
GeneIDi948766.
KEGGiecj:JW2507.
eco:b2523.
PATRICi32120441. VBIEscCol129921_2623.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84499 Genomic DNA. Translation: BAA12689.1.
U00096 Genomic DNA. Translation: AAC75576.2.
AP009048 Genomic DNA. Translation: BAA16413.1.
U01827 Unassigned DNA. No translation available.
PIRiB65029.
RefSeqiNP_417018.4. NC_000913.3.
WP_000133592.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP37095.
SMRiP37095. Positions 78-425.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261583. 5 interactions.
DIPiDIP-10455N.
IntActiP37095. 16 interactions.
MINTiMINT-1236207.
STRINGi511145.b2523.

Protein family/group databases

MEROPSiM17.004.

Proteomic databases

EPDiP37095.
PaxDbiP37095.
PRIDEiP37095.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75576; AAC75576; b2523.
BAA16413; BAA16413; BAA16413.
GeneIDi948766.
KEGGiecj:JW2507.
eco:b2523.
PATRICi32120441. VBIEscCol129921_2623.

Organism-specific databases

EchoBASEiEB2216.
EcoGeneiEG12310. pepB.

Phylogenomic databases

eggNOGiENOG4105BZ6. Bacteria.
COG0260. LUCA.
HOGENOMiHOG000243130.
InParanoidiP37095.
KOiK07751.
OMAiLARIECG.
PhylomeDBiP37095.

Enzyme and pathway databases

BioCyciEcoCyc:EG12310-MONOMER.
ECOL316407:JW2507-MONOMER.
MetaCyc:EG12310-MONOMER.
SABIO-RKP37095.

Miscellaneous databases

PROiP37095.

Family and domain databases

CDDicd00433. Peptidase_M17. 1 hit.
HAMAPiMF_00504. Aminopeptidase_M17. 1 hit.
InterProiIPR011356. Leucine_aapep/pepB.
IPR008330. Pept_M17_PepB.
IPR000819. Peptidase_M17_C.
[Graphical view]
PANTHERiPTHR11963. PTHR11963. 1 hit.
PfamiPF12404. DUF3663. 1 hit.
PF00883. Peptidase_M17. 1 hit.
[Graphical view]
PIRSFiPIRSF036388. Ctsl_amnpptdse_B. 1 hit.
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPEPB_ECOLI
AccessioniPrimary (citable) accession number: P37095
Secondary accession number(s): P76580
, P76989, P76991, Q47131
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.