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Protein

Amiloride-sensitive sodium channel subunit gamma

Gene

Scnn1g

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.By similarity1 Publication

Enzyme regulationi

Activated by WNK1, WNK2, WNK3 and WNK4.By similarity

GO - Molecular functioni

  • ligand-gated sodium channel activity Source: RGD
  • WW domain binding Source: RGD

GO - Biological processi

  • multicellular organismal water homeostasis Source: UniProtKB
  • response to hypoxia Source: RGD
  • sensory perception of taste Source: UniProtKB-KW
  • sodium ion homeostasis Source: UniProtKB
  • sodium ion transport Source: RGD
  • wound healing, spreading of epidermal cells Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Sodium channel

Keywords - Biological processi

Ion transport, Sensory transduction, Sodium transport, Taste, Transport

Keywords - Ligandi

Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Amiloride-sensitive sodium channel subunit gamma
Alternative name(s):
Epithelial Na(+) channel subunit gamma
Short name:
Gamma-ENaC
Gamma-NaCH
Nonvoltage-gated sodium channel 1 subunit gamma
SCNEG
Gene namesi
Name:Scnn1g
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3641. Scnn1g.

Subcellular locationi

  • Apical cell membrane 1 Publication; Multi-pass membrane protein By similarity

  • Note: Apical membrane of epithelial cells.1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5555CytoplasmicBy similarityAdd
BLAST
Transmembranei56 – 7621Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini77 – 542466ExtracellularBy similarityAdd
BLAST
Transmembranei543 – 56321Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini564 – 65087CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • apical plasma membrane Source: RGD
  • cell surface Source: RGD
  • integral component of plasma membrane Source: UniProtKB
  • sodium channel complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi6 – 61K → R: Interferes with ubiquitination and increases amiloride-sensitive current and the number of active channels; when associated with R-13. 1 Publication
Mutagenesisi13 – 131K → R: Interferes with ubiquitination and increases amiloride-sensitive current and the number of active channels; when associated with R-6. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 650650Amiloride-sensitive sodium channel subunit gammaPRO_0000181279Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi210 – 2101N-linked (GlcNAc...)Sequence analysis
Glycosylationi272 – 2721N-linked (GlcNAc...)Sequence analysis
Glycosylationi498 – 4981N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Phosphorylated on serine and threonine residues. Aldosterone and insulin increase the basal level of phosphorylation.1 Publication
Ubiquitinated; this targets individual subunits for endocytosis and proteasome-mediated degradation.1 Publication
ENaC cleavage by furin, and subsequently by prostasin (PRSS8), leads to a stepwise increase in the open probability of the channel as a result of release of the alpha and gamma subunit inhibitory tracts, respectively. Interaction of ENaC subunit SCNN1B with BPIFA1 protects ENaC against proteolytic activation.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP37091.
PRIDEiP37091.

PTM databases

iPTMnetiP37091.
PhosphoSiteiP37091.

Interactioni

Subunit structurei

Heterotrimer containing an alpha/SCNN1A, a beta/SCNN1B and a gamma/SCNN1G subunit. An additional delta/SCNN1D subunit exists only in some organisms and can replace the alpha/SCNN1A subunit to form an alternative channel with specific properties (Probable). Interacts with NEDD4; via the WW domains (PubMed:11323714). Interacts with NEDD4L; via the WW domains. Interacts with WWP1; via the WW domains. Interacts with WWP2; via the WW domains. Interacts with the full length immature form of PCSK9 (pro-PCSK9) (By similarity).By similarity1 Publication1 Publication

GO - Molecular functioni

  • WW domain binding Source: RGD

Protein-protein interaction databases

BioGridi246892. 8 interactions.
DIPiDIP-61327N.
IntActiP37091. 2 interactions.
STRINGi10116.ENSRNOP00000024057.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4294. Eukaryota.
ENOG410ZNFK. LUCA.
HOGENOMiHOG000236286.
HOVERGENiHBG058435.
InParanoidiP37091.
KOiK04827.
PhylomeDBiP37091.

Family and domain databases

InterProiIPR004724. ENaC.
IPR001873. Na+channel_ASC.
IPR020903. Na+channel_ASC_CS.
[Graphical view]
PANTHERiPTHR11690. PTHR11690. 2 hits.
PfamiPF00858. ASC. 1 hit.
[Graphical view]
PRINTSiPR01078. AMINACHANNEL.
TIGRFAMsiTIGR00859. ENaC. 1 hit.
PROSITEiPS01206. ASC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37091-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPGEKIKAK IKKNLPVRGP QAPTIKDLMH WYCMNTNTHG CRRIVVSRGR
60 70 80 90 100
LRRLLWIAFT LTAVALIIWQ CALLVFSFYT VSVSIKVHFQ KLDFPAVTIC
110 120 130 140 150
NINPYKYSAV SDLLTDLDSE TKQALLSLYG VKESRKRREA GSMPSTLEGT
160 170 180 190 200
PPRFFKLIPL LVFNENEKGK ARDFFTGRKR KISGKIIHKA SNVMHVHESK
210 220 230 240 250
KLVGFQLCSN DTSDCATYTF SSGINAIQEW YKLHYMNIMA QVPLEKKINM
260 270 280 290 300
SYSAEELLVT CFFDGMSCDA RNFTLFHHPM YGNCYTFNNK ENATILSTSM
310 320 330 340 350
GGSEYGLQVI LYINEDEYNP FLVSSTGAKV LIHQQNEYPF IEDVGMEIET
360 370 380 390 400
AMSTSIGMHL TESFKLSEPY SQCTEDGSDV PVTNIYNAAY SLQICLYSCF
410 420 430 440 450
QTKMVEKCGC AQYSQPLPPA ANYCNYQQHP NWMYCYYQLY QAFVREELGC
460 470 480 490 500
QSVCKQSCSF KEWTLTTSLA QWPSEASEKW LLNVLTWDQS QQINKKLNKT
510 520 530 540 550
DLAKLLIFYK DLNQRSIMES PANSIEMLLS NFGGQLGLWM SCSVVCVIEI
560 570 580 590 600
IEVFFIDFFS IIARRQWHKA KDWWARRQTP PSTETPSSRQ GQDNPALDTD
610 620 630 640 650
DDLPTFTSAM RLPPAPGSTV PGTPPPRYNT LRLDRAFSSQ LTDTQLTNEL
Length:650
Mass (Da):74,066
Last modified:November 1, 1997 - v2
Checksum:i701F9B28B3250D8F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti53 – 531R → P in CAA54905 (PubMed:8107805).Curated
Sequence conflicti573 – 5731W → C in CAA54905 (PubMed:8107805).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77933 mRNA. Translation: CAA54905.1.
X78034 mRNA. Translation: CAA54964.1.
U37539 mRNA. Translation: AAB58459.1.
U37540 mRNA. Translation: AAB58460.1.
PIRiA54065.
RefSeqiNP_058742.1. NM_017046.1.
UniGeneiRn.10360.

Genome annotation databases

GeneIDi24768.
KEGGirno:24768.
UCSCiRGD:3641. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77933 mRNA. Translation: CAA54905.1.
X78034 mRNA. Translation: CAA54964.1.
U37539 mRNA. Translation: AAB58459.1.
U37540 mRNA. Translation: AAB58460.1.
PIRiA54065.
RefSeqiNP_058742.1. NM_017046.1.
UniGeneiRn.10360.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246892. 8 interactions.
DIPiDIP-61327N.
IntActiP37091. 2 interactions.
STRINGi10116.ENSRNOP00000024057.

PTM databases

iPTMnetiP37091.
PhosphoSiteiP37091.

Proteomic databases

PaxDbiP37091.
PRIDEiP37091.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24768.
KEGGirno:24768.
UCSCiRGD:3641. rat.

Organism-specific databases

CTDi6340.
RGDi3641. Scnn1g.

Phylogenomic databases

eggNOGiKOG4294. Eukaryota.
ENOG410ZNFK. LUCA.
HOGENOMiHOG000236286.
HOVERGENiHBG058435.
InParanoidiP37091.
KOiK04827.
PhylomeDBiP37091.

Miscellaneous databases

PROiP37091.

Family and domain databases

InterProiIPR004724. ENaC.
IPR001873. Na+channel_ASC.
IPR020903. Na+channel_ASC_CS.
[Graphical view]
PANTHERiPTHR11690. PTHR11690. 2 hits.
PfamiPF00858. ASC. 1 hit.
[Graphical view]
PRINTSiPR01078. AMINACHANNEL.
TIGRFAMsiTIGR00859. ENaC. 1 hit.
PROSITEiPS01206. ASC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSCNNG_RAT
AccessioniPrimary (citable) accession number: P37091
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1997
Last modified: July 6, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.