Reviewed, UniProtKB/Swiss-Prot P37091 (SCNNG_RAT)

Names and origin

Protein names

Recommended name:
    Amiloride-sensitive sodium channel subunit gamma
Alternative name(s):
    Epithelial Na(+) channel subunit gamma
    Gamma-ENaC
    SCNEG
    Nonvoltage-gated sodium channel 1 subunit gamma
    Gamma-NaCH

Gene names

Name:

Scnn1g

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	650 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.
Subunit structure	Heterotetramer of two alpha, one beta and one gamma subunit. A delta subunit can replace the alpha subunit. Interacts with the WW domains of NEDD4, NEDD4L, WWP1 and WWP2 By similarity.
Subcellular location	Apical cell membrane; Multi-pass membrane protein By similarity. Note: Apical membrane of epithelial cells By similarity.
Post-translational modification	Phosphorylated on serine and threonine residues. Aldosterone and insulin increase the basal level of phosphorylation. Ref.5 Ubiquitinated; this targets individual subunits for endocytosis and proteasome-mediated degradation. Ref.4
Sequence similarities	Belongs to the amiloride-sensitive sodium channel family.

Ontologies

Keywords
Biological process	Ion transport Sensory transduction Sodium transport Taste Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	Sodium
Molecular function	Ionic channel Sodium channel
PTM	Glycoprotein Phosphoprotein Ubl conjugation
Gene Ontology (GO)
Biological process	response to hypoxia Inferred from expression pattern. Source: RGD sensory perception of taste Inferred from electronic annotation. Source: UniProtKB-KW sodium ion transport Inferred from direct assay. Source: RGD wound healing, spreading of epidermal cells Inferred from mutant phenotype. Source: RGD
Cellular component	apical plasma membrane Inferred from direct assay. Source: RGD
Molecular function	WW domain binding Inferred from physical interaction. Source: RGD amiloride-sensitive sodium channel activity Inferred from direct assay. Source: RGD sodium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 650	650	Amiloride-sensitive sodium channel subunit gamma		PRO_0000181279
Regions
	Topological domain	1 – 55	55	Cytoplasmic Potential
	Transmembrane	56 – 76	21	Potential
	Topological domain	77 – 542	466	Extracellular Potential
	Transmembrane	543 – 563	21	Potential
	Topological domain	564 – 650	87	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	210	1	N-linked (GlcNAc...) Potential
	Glycosylation	249	1	N-linked (GlcNAc...) Potential
	Glycosylation	272	1	N-linked (GlcNAc...) Potential
	Glycosylation	292	1	N-linked (GlcNAc...) Potential
	Glycosylation	498	1	N-linked (GlcNAc...) Potential
Experimental info
	Mutagenesis	6	1	K → R: Interferes with ubiquitination and increases amiloride-sensitive current and the number of active channels; when associated with R-13. Ref.4
	Mutagenesis	13	1	K → R: Interferes with ubiquitination and increases amiloride-sensitive current and the number of active channels; when associated with R-6. Ref.4
	Sequence conflict	53	1	R → P in CAA54905. Ref.1
	Sequence conflict	573	1	W → C in CAA54905. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P37091-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 701F9B28B3250D8F	FASTA	650	74,066
	10 20 30 40 50 60 MAPGEKIKAK IKKNLPVRGP QAPTIKDLMH WYCMNTNTHG CRRIVVSRGR LRRLLWIAFT 70 80 90 100 110 120 LTAVALIIWQ CALLVFSFYT VSVSIKVHFQ KLDFPAVTIC NINPYKYSAV SDLLTDLDSE 130 140 150 160 170 180 TKQALLSLYG VKESRKRREA GSMPSTLEGT PPRFFKLIPL LVFNENEKGK ARDFFTGRKR 190 200 210 220 230 240 KISGKIIHKA SNVMHVHESK KLVGFQLCSN DTSDCATYTF SSGINAIQEW YKLHYMNIMA 250 260 270 280 290 300 QVPLEKKINM SYSAEELLVT CFFDGMSCDA RNFTLFHHPM YGNCYTFNNK ENATILSTSM 310 320 330 340 350 360 GGSEYGLQVI LYINEDEYNP FLVSSTGAKV LIHQQNEYPF IEDVGMEIET AMSTSIGMHL 370 380 390 400 410 420 TESFKLSEPY SQCTEDGSDV PVTNIYNAAY SLQICLYSCF QTKMVEKCGC AQYSQPLPPA 430 440 450 460 470 480 ANYCNYQQHP NWMYCYYQLY QAFVREELGC QSVCKQSCSF KEWTLTTSLA QWPSEASEKW 490 500 510 520 530 540 LLNVLTWDQS QQINKKLNKT DLAKLLIFYK DLNQRSIMES PANSIEMLLS NFGGQLGLWM 550 560 570 580 590 600 SCSVVCVIEI IEVFFIDFFS IIARRQWHKA KDWWARRQTP PSTETPSSRQ GQDNPALDTD 610 620 630 640 650 DDLPTFTSAM RLPPAPGSTV PGTPPPRYNT LRLDRAFSSQ LTDTQLTNEL

References

[1]	"Amiloride-sensitive epithelial Na+ channel is made of three homologous subunits." Canessa C.M., Schild L., Buell G., Thorens B., Gautschi I., Horisberger J.-D., Rossier B.C. Nature 367:463-467(1994) [PubMed: 8107805] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Distal colon epithelium.
[2]	"Different homologous subunits of the amiloride-sensitive Na+ channel are differently regulated by aldosterone." Lingueglia R., Renard S., Waldmann R., Voilley N., Champigny G., Plass H., Lazdunski M., Barbry P. J. Biol. Chem. 269:13736-13739(1994) [PubMed: 8188647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Distal colon.
[3]	"Role of the alpha-, beta-, and gamma-subunits of epithelial sodium channel in a model of polygenic hypertension." Kreutz R., Struk B., Rubattu S., Hubner N., Szpirer J., Szpirer C., Ganten D., Lindpaintner K. Hypertension 29:131-136(1997) [PubMed: 9039092] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Distal colon and Kidney.
[4]	"Regulation of stability and function of the epithelial Na+ channel (ENaC) by ubiquitination." Staub O., Gautschi I., Ishikawa T., Breitschopf K., Ciechanover A., Schild L., Rotin D. EMBO J. 16:6325-6336(1997) [PubMed: 9351815] [Abstract] Cited for: MUTAGENESIS OF LYS-6 AND LYS-13, UBIQUITINATION.
[5]	"In vivo phosphorylation of the epithelial sodium channel." Shimkets R.A., Lifton R., Canessa C.M. Proc. Natl. Acad. Sci. U.S.A. 95:3301-3305(1998) [PubMed: 9501257] [Abstract] Cited for: PHOSPHORYLATION.
[6]	"Solution structure of a Nedd4 WW domain-ENaC peptide complex." Kanelis V., Rotin D., Forman-Kay J.D. Nat. Struct. Biol. 8:407-412(2001) [PubMed: 11323714] [Abstract] Cited for: INTERACTION WITH NEDD4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X77933 mRNA. Translation: CAA54905.1. X78034 mRNA. Translation: CAA54964.1. U37539 mRNA. Translation: AAB58459.1. U37540 mRNA. Translation: AAB58460.1.
IPI	IPI00325745.
PIR	A54065.
RefSeq	NP_058742.1.
UniGene	Rn.10360
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P37091.
PTM databases
PhosphoSite	P37091.
Proteomic databases
PRIDE	P37091.
Genome annotation databases
Ensembl	ENSRNOT00000024057; ENSRNOP00000024057; ENSRNOG00000017842; Rattus norvegicus. [Genome view]
GeneID	24768.
KEGG	rno:24768.
UCSC	NM_017046. rat.
Organism-specific databases
CTD	24768.
RGD	3641. Scnn1g.
Phylogenomic databases
HOVERGEN	P37091.
Gene expression databases
ArrayExpress	P37091.
Genevestigator	P37091.
GermOnline	ENSRNOG00000017842. Rattus norvegicus.
Family and domain databases
InterPro	IPR004724. EnaC. IPR001873. Na+channel_ASC. [Graphical view]
PANTHER	PTHR11690. Na+channel_ASC. 1 hit.
Pfam	PF00858. ASC. 1 hit. [Graphical view]
PRINTS	PR01078. AMINACHANNEL.
TIGRFAMs	TIGR00859. ENaC. 1 hit.
PROSITE	PS01206. ASC. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	604332.

Entry information

Entry name

SCNNG_RAT

Accession

Primary (citable) accession number: P37091

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	November 1, 1997
Last modified:	October 13, 2009
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)