UniProtKB - P37090 (SCNNB_RAT)
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Protein
Amiloride-sensitive sodium channel subunit beta
Gene
Scnn1b
Organism
Rattus norvegicus (Rat)
Status
Functioni
Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.By similarity
Enzyme regulationi
Activated by WNK1, WNK2, WNK3 and WNK4.By similarity
GO - Molecular functioni
- ligand-gated sodium channel activity Source: RGD
- WW domain binding Source: RGD
GO - Biological processi
- multicellular organismal water homeostasis Source: UniProtKB
- regulation of sodium ion transport Source: RGD
- response to hypoxia Source: RGD
- sensory perception of taste Source: UniProtKB-KW
- sodium ion homeostasis Source: UniProtKB
- sodium ion transmembrane transport Source: RGD
- sodium ion transport Source: RGD
- wound healing, spreading of epidermal cells Source: RGD
Keywordsi
Molecular function | Ion channel, Sodium channel |
Biological process | Ion transport, Sensory transduction, Sodium transport, Taste, Transport |
Ligand | Sodium |
Names & Taxonomyi
Protein namesi | Recommended name: Amiloride-sensitive sodium channel subunit betaAlternative name(s): Beta-NaCH Epithelial Na(+) channel subunit beta Short name: Beta-ENaC Nonvoltage-gated sodium channel 1 subunit beta SCNEB |
Gene namesi | Name:Scnn1b |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3640. Scnn1b. |
Subcellular locationi
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 50 | CytoplasmicBy similarityAdd BLAST | 50 | |
Transmembranei | 51 – 71 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 72 – 530 | ExtracellularBy similarityAdd BLAST | 459 | |
Transmembranei | 531 – 551 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 552 – 638 | CytoplasmicBy similarityAdd BLAST | 87 |
Keywords - Cellular componenti
Cell membrane, Cytoplasmic vesicle, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 616 | P → A: Strong increase in channel activity. 1 Publication | 1 | |
Mutagenesisi | 618 | Y → A: Strong increase in channel activity. 1 Publication | 1 | |
Mutagenesisi | 621 | L → A: 2-fold increase in channel activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000181271 | 1 – 638 | Amiloride-sensitive sodium channel subunit betaAdd BLAST | 638 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 135 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 141 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 631 | PhosphoserineBy similarity | 1 | |
Modified residuei | 633 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
N-glycosylated (PubMed:11773057). N-glycosylation is required for interaction with BPIFA1 (By similarity).By similarity1 Publication
Phosphorylated on serine and threonine residues. Aldosterone and insulin increase the basal level of phosphorylation.1 Publication
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | P37090. |
PTM databases
CarbonylDBi | P37090. |
iPTMneti | P37090. |
PhosphoSitePlusi | P37090. |
Interactioni
Subunit structurei
Heterotrimer containing an alpha/SCNN1A, a beta/SCNN1B and a gamma/SCNN1G subunit. An additional delta/SCNN1D subunit exists only in some organisms and can replace the alpha/SCNN1A subunit to form an alternative channel with specific properties (By similarity). Interacts with NEDD4 (via WW domains) (PubMed:12654927, PubMed:11323714). Interacts with NEDD4L (via WW domains) (By similarity). Interacts with WWP1 (via WW domains) (By similarity). Interacts with WWP2 (via WW domains) (By similarity). Interacts with the full-length immature form of PCSK9 (pro-PCSK9) (By similarity). Interacts (N-glycosylated) with BPIFA1; the interaction is direct and inhibits the proteolytic processing of SCNN1A and SCNN1G and the activation of ENaC (By similarity).By similarity2 Publications
GO - Molecular functioni
- WW domain binding Source: RGD
Protein-protein interaction databases
BioGridi | 246891. 2 interactors. |
IntActi | P37090. 2 interactors. |
STRINGi | 10116.ENSRNOP00000063755. |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more detailsFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Turni | 618 – 620 | Combined sources | 3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1I5H | NMR | - | B | 607-621 | [»] | |
SMRi | P37090. | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P37090. |
Family & Domainsi
Sequence similaritiesi
Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1B subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4294. Eukaryota. ENOG410ZNFK. LUCA. |
HOGENOMi | HOG000236286. |
HOVERGENi | HBG058435. |
InParanoidi | P37090. |
KOi | K04825. |
PhylomeDBi | P37090. |
Family and domain databases
InterProi | View protein in InterPro IPR001873. ENaC. IPR004724. ENaC_chordates. IPR020903. ENaC_CS. |
PANTHERi | PTHR11690. PTHR11690. 1 hit. |
Pfami | View protein in Pfam PF00858. ASC. 1 hit. |
PRINTSi | PR01078. AMINACHANNEL. |
TIGRFAMsi | TIGR00859. ENaC. 1 hit. |
PROSITEi | View protein in PROSITE PS01206. ASC. 1 hit. |
i Sequence
Sequence statusi: Complete.
P37090-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPVKKYLLKC LHRLQKGPGY TYKELLVWYC NNTNTHGPKR IICEGPKKKA
60 70 80 90 100
MWFLLTLLFA CLVCWQWGVF IQTYLSWEVS VSLSMGFKTM NFPAVTVCNS
110 120 130 140 150
SPFQYSKVKH LLKDLYKLME AVLDKILAPK SSHTNTTSTL NFTIWNHTPL
160 170 180 190 200
VLIDERNPDH PVVLNLFGDS HNSSNPAPGS TCNAQGCKVA MRLCSANGTV
210 220 230 240 250
CTFRNFTSAT QAVTEWYILQ ATNIFSQVLP QDLVGMGYAP DRIILACLFG
260 270 280 290 300
TEPCSHRNFT PIFYPDYGNC YIFNWGMTEK ALPSANPGTE FGLKLILDIG
310 320 330 340 350
QEDYVPFLAS TAGARLMLHE QRTYPFIREE GIYAMAGTET SIGVLLDKLQ
360 370 380 390 400
GKGEPYSPCT MNGSDVAIQN LYSDYNTTYS IQACLHSCFQ DHMIHNCSCG
410 420 430 440 450
HYLYPLPAGE KYCNNRDFPD WAYCYLSLQM SVVQRETCLS MCKESCNDTQ
460 470 480 490 500
YKMTISMADW PSEASEDWIL HVLSQERDQS SNITLSRKGI VKLNIYFQEF
510 520 530 540 550
NYRTIEESPA NNIVWLLSNL GGQFGFWMGG SVLCLIEFGE IIIDFIWITV
560 570 580 590 600
IKLVASCKGL RRRRPQAPYT GPPPTVAELV EAHTNFGFQP DTTSCRPNAE
610 620 630
VYPDQQTLPI PGTPPPNYDS LRLQPLDTME SDSEVEAI
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 567 | A → R in CAA54904 (PubMed:8107805).Curated | 1 | |
Sequence conflicti | 586 – 587 | FG → CV in CAA54904 (PubMed:8107805).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X77932 mRNA. Translation: CAA54904.1. U35174 mRNA. Translation: AAB58457.1. U35175 mRNA. Translation: AAB58458.1. |
PIRi | S41159. |
RefSeqi | NP_036780.1. NM_012648.1. |
UniGenei | Rn.9807. |
Genome annotation databases
GeneIDi | 24767. |
KEGGi | rno:24767. |
UCSCi | RGD:3640. rat. |
Similar proteinsi
Entry informationi
Entry namei | SCNNB_RAT | |
Accessioni | P37090Primary (citable) accession number: P37090 Secondary accession number(s): O09183 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 28, 2018 | |
This is version 136 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |