Reviewed, UniProtKB/Swiss-Prot P37075 (SCRB_SALTY)

Names and origin

Protein names

Recommended name:
    Sucrose-6-phosphate hydrolase
      Short name=Sucrase
    EC=3.2.1.26
Alternative name(s):
    Invertase

Gene names

Name:

scrB

Encoded on

Plasmid pUR400

Organism

Salmonella typhimurium

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Enables the bacterium to metabolize sucrose as a sole carbon source.
Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Pathway	Glycan biosynthesis; sucrose metabolism.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-fructofuranosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	Sucrose-6-phosphate hydrolase		PRO_0000169877
Regions
	Region	38 – 41	4	Substrate binding By similarity
	Region	100 – 101	2	Substrate binding By similarity
	Region	159 – 160	2	Substrate binding By similarity
Sites
	Active site	41	1	By similarity
	Binding site	57	1	Substrate By similarity
	Binding site	218	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P37075-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: A62CB3F53726138B	FASTA	466	52,589
	10 20 30 40 50 60 MSLPSRLPAI LQAVMQGQPR ALADSHYPRW HHAPVTGLMN DPNGFIEFAG RYHLFYQWNP 70 80 90 100 110 120 LACDHTFKCW AHWSSIDLLH WQHEPIALMP DEEYDRNGCY SGSAVDNNGT LTLCYTGNVK 130 140 150 160 170 180 FAEGGRTAWQ CLATENADGT FRKIGPVLPL PEGYTGHVRD PKVWRHEDLW YMVLGAQDRQ 190 200 210 220 230 240 KRGKVLLFSS ADLHQWTSMG EIAGHGINGL DDVGYMWECP DLFPLGDQHI LICCPQGIAR 250 260 270 280 290 300 EEECYLNTYP AVWMAGEFDY AAGAFRHGEL HELDAGFEFY APQTMLTSDG RRLLVGWMGV 310 320 330 340 350 360 PEGEEMLQPT LNNGWIHQMT CLRELEFING QLYQRPLREL SALRGEANGW SGNALPLAPM 370 380 390 400 410 420 EIDLQTRGGD MLSLDFGGVL TLECDASGLR LARRSLASDE MHYRYWRGNV RSLRVFIDQS 430 440 450 460 SVEIFINGGE GVMSSRYFPA CSGQLTFSGI TPDAFCYWPL RTCMVE

References

[1]	"Molecular analysis of two ScrR repressors and of a ScrR-FruR hybrid repressor for sucrose and D-fructose specific regulons from enteric bacteria." Jahreis K., Lengeler J.W. Mol. Microbiol. 9:195-209(1993) [PubMed: 8412665] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Molecular analysis of the scrA and scrB genes from Klebsiella pneumoniae and plasmid pUR400, which encode the sucrose transport protein Enzyme II Scr of the phosphotransferase system and a sucrose-6-phosphate invertase." Titgemeyer F., Jahreis K., Ebner R., Lengeler J.W. Mol. Gen. Genet. 250:197-206(1996) [PubMed: 8628219] [Abstract] Cited for: SEQUENCE REVISION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X67750 Genomic DNA. Translation: CAA47974.1.
PIR	S62330.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
Enzyme and pathway databases
BRENDA	3.2.1.26. 2.
Family and domain databases
InterPro	IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. IPR006232. Suc6P_hydrolase. [Graphical view]
Pfam	PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
TIGRFAMs	TIGR01322. scrB_fam. 1 hit.
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SCRB_SALTY

Accession

Primary (citable) accession number: P37075

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)