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P37073 (GUB_BREBE) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-glucanase

EC=3.2.1.73
Alternative name(s):
1,3-1,4-beta-D-glucan 4-glucanohydrolase
Endo-beta-1,3-1,4 glucanase
Lichenase
Gene names
Name:bglBB
OrganismBrevibacillus brevis (Bacillus brevis)
Taxonomic identifier1393 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesPaenibacillaceaeBrevibacillus

Protein attributes

Sequence length259 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes B-glucans containing mixed beta-1,3 and beta-1,4 linkages.

Catalytic activity

Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.

Miscellaneous

Beta-glucanases of Bacillus have a substrate range similar to lichenase of germinating barley.

Sequence similarities

Belongs to the glycosyl hydrolase 16 family.

Biophysicochemical properties

Temperature dependence:

Optimum temperature is 65-70 degrees Celsius. Thermostable.

Ontologies

Keywords
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionlicheninase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 Potential
Chain32 – 259228Beta-glucanase
PRO_0000011787

Sites

Active site1421Nucleophile By similarity
Active site1461Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
P37073 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: A63C09F281FF5D13

FASTA25929,960
        10         20         30         40         50         60 
MVKSKYLVFI SVFSLLFGVF VVGFSHQGVK AEEERPMGTA FYESFDAFDD ERWSKAGVWT 

        70         80         90        100        110        120 
NGQMFNATWY PEQVTADGLM RLTIAKKTTS ARNYKAGELR TNDFYHYGLF EVSMKPAKVE 

       130        140        150        160        170        180 
GTVSSFFTYT GEWDWDGDPW DEIDIEFLGK DTTRIQFNYF TNGVGGNEFY YDLGFDASES 

       190        200        210        220        230        240 
FNTYAFEWRE DSITWYVNGE AVHTATENIP QTPQKIMMNL WPGVGVDGWT GVFDGDNTPV 

       250 
YSYYDWVRYT PLQNYQIHQ 

« Hide

References

[1]"Characterization, cloning and sequencing of a thermostable endo-(1,3-1,4) beta-glucanase-encoding gene from an alkalophilic Bacillus brevis."
Louw M.E., Reid S.J., Watson T.G.
Appl. Microbiol. Biotechnol. 38:507-513(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Alk36.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M84339 Genomic DNA. Translation: AAA22265.1.
PIRA48378.

3D structure databases

ProteinModelPortalP37073.
SMRP37073. Positions 37-253.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR008264. Beta_glucanase.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
[Graphical view]
PfamPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
PRINTSPR00737. GLHYDRLASE16.
SUPFAMSSF49899. SSF49899. 1 hit.
PROSITEPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUB_BREBE
AccessionPrimary (citable) accession number: P37073
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: October 16, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries