Reviewed, UniProtKB/Swiss-Prot P37073 (GUB_BREBE)

Names and origin

Protein names

Recommended name:
    Beta-glucanase
    EC=3.2.1.73
Alternative name(s):
    Endo-beta-1,3-1,4 glucanase
    1,3-1,4-beta-D-glucan 4-glucanohydrolase
    Lichenase

Gene names

Name:

bglBB

Organism

Brevibacillus brevis (Bacillus brevis)

Taxonomic identifier

1393 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Paenibacillaceae › Brevibacillus

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Hydrolyzes B-glucans containing mixed beta-1,3 and beta-1,4 linkages.
Catalytic activity	Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.
Miscellaneous	Beta-glucanases of Bacillus have a substrate range similar to lichenase of germinating barley.
Sequence similarities	Belongs to the glycosyl hydrolase 16 family.
biophysicochemical properties	Temperature dependence: Optimum temperature is 65-70 degrees Celsius. Thermostable.

Ontologies

Keywords
Domain	Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	licheninase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 259	228	Beta-glucanase		PRO_0000011787
Sites
	Active site	142	1	Nucleophile By similarity
	Active site	146	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P37073-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: A63C09F281FF5D13	FASTA	259	29,960
	10 20 30 40 50 60 MVKSKYLVFI SVFSLLFGVF VVGFSHQGVK AEEERPMGTA FYESFDAFDD ERWSKAGVWT 70 80 90 100 110 120 NGQMFNATWY PEQVTADGLM RLTIAKKTTS ARNYKAGELR TNDFYHYGLF EVSMKPAKVE 130 140 150 160 170 180 GTVSSFFTYT GEWDWDGDPW DEIDIEFLGK DTTRIQFNYF TNGVGGNEFY YDLGFDASES 190 200 210 220 230 240 FNTYAFEWRE DSITWYVNGE AVHTATENIP QTPQKIMMNL WPGVGVDGWT GVFDGDNTPV 250 YSYYDWVRYT PLQNYQIHQ

References

[1]

"Characterization, cloning and sequencing of a thermostable endo-(1,3-1,4) beta-glucanase-encoding gene from an alkalophilic Bacillus brevis." Louw M.E., Reid S.J., Watson T.G. Appl. Microbiol. Biotechnol. 38:507-513(1993) [PubMed: 7763386] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Alk36.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M84339 Genomic DNA. Translation: AAA22265.1.
PIR	A48378.
3D structure databases
HSSP	HSSP built from PDB template 1AJK based on UniProtKB P23904.
ModBase	Search...
Protein family/group databases
CAZy	GH16. Glycoside Hydrolase Family 16.
Enzyme and pathway databases
BRENDA	3.2.1.73. 289125.
Family and domain databases
InterPro	IPR008264. Beta_glucanase. IPR013320. ConA-like_subgrp. IPR000757. Glyco_hydro_16. IPR008263. Glycoside_hydrolase_16_AS. [Graphical view]
Gene3D	G3DSA:2.60.120.200. ConA_like_subgrp. 1 hit.
Pfam	PF00722. Glyco_hydro_16. 1 hit. [Graphical view]
PRINTS	PR00737. GLHYDRLASE16.
PROSITE	PS01034. GLYCOSYL_HYDROL_F16. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUB_BREBE

Accession

Primary (citable) accession number: P37073

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)