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P37073

- GUB_BREBE

UniProt

P37073 - GUB_BREBE

Protein

Beta-glucanase

Gene

bglBB

Organism
Brevibacillus brevis (Bacillus brevis)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Hydrolyzes B-glucans containing mixed beta-1,3 and beta-1,4 linkages.

    Catalytic activityi

    Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.

    Temperature dependencei

    Optimum temperature is 65-70 degrees Celsius. Thermostable.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei142 – 1421NucleophilePROSITE-ProRule annotation
    Active sitei146 – 1461Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. licheninase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH16. Glycoside Hydrolase Family 16.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-glucanase (EC:3.2.1.73)
    Alternative name(s):
    1,3-1,4-beta-D-glucan 4-glucanohydrolase
    Endo-beta-1,3-1,4 glucanase
    Lichenase
    Gene namesi
    Name:bglBB
    OrganismiBrevibacillus brevis (Bacillus brevis)
    Taxonomic identifieri1393 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaeBrevibacillus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3131Sequence AnalysisAdd
    BLAST
    Chaini32 – 259228Beta-glucanasePRO_0000011787Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP37073.
    SMRiP37073. Positions 37-253.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 16 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR008264. Beta_glucanase.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    [Graphical view]
    PfamiPF00722. Glyco_hydro_16. 1 hit.
    [Graphical view]
    PRINTSiPR00737. GLHYDRLASE16.
    SUPFAMiSSF49899. SSF49899. 1 hit.
    PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P37073-1 [UniParc]FASTAAdd to Basket

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    MVKSKYLVFI SVFSLLFGVF VVGFSHQGVK AEEERPMGTA FYESFDAFDD    50
    ERWSKAGVWT NGQMFNATWY PEQVTADGLM RLTIAKKTTS ARNYKAGELR 100
    TNDFYHYGLF EVSMKPAKVE GTVSSFFTYT GEWDWDGDPW DEIDIEFLGK 150
    DTTRIQFNYF TNGVGGNEFY YDLGFDASES FNTYAFEWRE DSITWYVNGE 200
    AVHTATENIP QTPQKIMMNL WPGVGVDGWT GVFDGDNTPV YSYYDWVRYT 250
    PLQNYQIHQ 259
    Length:259
    Mass (Da):29,960
    Last modified:June 1, 1994 - v1
    Checksum:iA63C09F281FF5D13
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M84339 Genomic DNA. Translation: AAA22265.1.
    PIRiA48378.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M84339 Genomic DNA. Translation: AAA22265.1 .
    PIRi A48378.

    3D structure databases

    ProteinModelPortali P37073.
    SMRi P37073. Positions 37-253.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH16. Glycoside Hydrolase Family 16.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR008264. Beta_glucanase.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    [Graphical view ]
    Pfami PF00722. Glyco_hydro_16. 1 hit.
    [Graphical view ]
    PRINTSi PR00737. GLHYDRLASE16.
    SUPFAMi SSF49899. SSF49899. 1 hit.
    PROSITEi PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization, cloning and sequencing of a thermostable endo-(1,3-1,4) beta-glucanase-encoding gene from an alkalophilic Bacillus brevis."
      Louw M.E., Reid S.J., Watson T.G.
      Appl. Microbiol. Biotechnol. 38:507-513(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Alk36.

    Entry informationi

    Entry nameiGUB_BREBE
    AccessioniPrimary (citable) accession number: P37073
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Beta-glucanases of Bacillus have a substrate range similar to lichenase of germinating barley.

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3