SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P37059

- DHB2_HUMAN

UniProt

P37059 - DHB2_HUMAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Estradiol 17-beta-dehydrogenase 2
Gene
HSD17B2, EDH17B2
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH.

Catalytic activityi

17-beta-estradiol + NAD(P)+ = estrone + NAD(P)H.
Testosterone + NAD+ = androstenedione + NADH.

Kineticsi

  1. KM=0.21 µM for estradiol1 Publication
  2. KM=0.39 µM for testosterone
  3. KM=0.31 µM for dihydrotestosterone
  4. KM=0.71 µM for 20-alpha-dihydroprogesterone
  5. KM=0.78 µM for estrone
  6. KM=2.63 µM for androstenedione

Vmax=38 nmol/min/mg enzyme with estradiol as substrate

Vmax=45 nmol/min/mg enzyme with testosterone as substrate

Vmax=38 nmol/min/mg enzyme with dihydrotestosterone as substrate

Vmax=5.6 nmol/min/mg enzyme with 20-alpha-dihydroprogesterone as substrate

Vmax=6.6 nmol/min/mg enzyme with estrone as substrate

Vmax=11.5 nmol/min/mg enzyme with androstenedione as substrate

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei219 – 2191Substrate By similarity
Active sitei232 – 2321 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi82 – 11130NAD By similarity
Add
BLAST

GO - Molecular functioni

  1. 17-alpha,20-alpha-dihydroxypregn-4-en-3-one dehydrogenase activity Source: BHF-UCL
  2. estradiol 17-beta-dehydrogenase activity Source: UniProtKB
  3. testosterone dehydrogenase (NAD+) activity Source: UniProtKB

GO - Biological processi

  1. in utero embryonic development Source: Ensembl
  2. placenta development Source: Ensembl
  3. response to retinoic acid Source: BHF-UCL
  4. steroid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Estradiol 17-beta-dehydrogenase 2 (EC:1.1.1.62)
Alternative name(s):
17-beta-hydroxysteroid dehydrogenase type 2
Short name:
17-beta-HSD 2
20 alpha-hydroxysteroid dehydrogenase
Short name:
20-alpha-HSD
E2DH
Microsomal 17-beta-hydroxysteroid dehydrogenase
Testosterone 17-beta-dehydrogenase (EC:1.1.1.239)
Gene namesi
Name:HSD17B2
Synonyms:EDH17B2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 16

Organism-specific databases

HGNCiHGNC:5211. HSD17B2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei4 – 2421Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: ProtInc
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29479.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 387387Estradiol 17-beta-dehydrogenase 2
PRO_0000054570Add
BLAST

Proteomic databases

MaxQBiP37059.
PaxDbiP37059.
PeptideAtlasiP37059.
PRIDEiP37059.

PTM databases

PhosphoSiteiP37059.

Expressioni

Gene expression databases

ArrayExpressiP37059.
BgeeiP37059.
CleanExiHS_HSD17B2.
GenevestigatoriP37059.

Organism-specific databases

HPAiHPA021826.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000199936.

Structurei

3D structure databases

ProteinModelPortaliP37059.
SMRiP37059. Positions 83-316.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1028.
HOVERGENiHBG005482.
InParanoidiP37059.
KOiK13368.
OMAiQEDYGQD.
PhylomeDBiP37059.
TreeFamiTF325617.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37059-1 [UniParc]FASTAAdd to Basket

« Hide

MSTFFSDTAW ICLAVPTVLC GTVFCKYKKS SGQLWSWMVC LAGLCAVCLL    50
ILSPFWGLIL FSVSCFLMYT YLSGQELLPV DQKAVLVTGG DCGLGHALCK 100
YLDELGFTVF AGVLNENGPG AEELRRTCSP RLSVLQMDIT KPVQIKDAYS 150
KVAAMLQDRG LWAVINNAGV LGFPTDGELL LMTDYKQCMA VNFFGTVEVT 200
KTFLPLLRKS KGRLVNVSSM GGGAPMERLA SYGSSKAAVT MFSSVMRLEL 250
SKWGIKVASI QPGGFLTNIA GTSDKWEKLE KDILDHLPAE VQEDYGQDYI 300
LAQRNFLLLI NSLASKDFSP VLRDIQHAIL AKSPFAYYTP GKGAYLWICL 350
AHYLPIGIYD YFAKRHFGQD KPMPRALRMP NYKKKAT 387
Length:387
Mass (Da):42,785
Last modified:June 1, 1994 - v1
Checksum:iEDE5606EA041042B
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti121 – 1211A → T.1 Publication
Corresponds to variant rs8191136 [ dbSNP | Ensembl ].
VAR_018852

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L11708 mRNA. Translation: AAA03562.1.
L40802
, L40796, L40797, L40798, L40801 Genomic DNA. Translation: AAC41917.1.
AY325910 Genomic DNA. Translation: AAP78485.1.
AK313109 mRNA. Translation: BAG35931.1.
CH471114 Genomic DNA. Translation: EAW95519.1.
BC009581 mRNA. Translation: AAH09581.1.
BC059170 mRNA. Translation: AAH59170.1.
CCDSiCCDS10936.1.
PIRiA47287.
RefSeqiNP_002144.1. NM_002153.2.
UniGeneiHs.162795.

Genome annotation databases

EnsembliENST00000199936; ENSP00000199936; ENSG00000086696.
GeneIDi3294.
KEGGihsa:3294.
UCSCiuc002fgv.3. human.

Polymorphism databases

DMDMi544152.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L11708 mRNA. Translation: AAA03562.1 .
L40802
, L40796 , L40797 , L40798 , L40801 Genomic DNA. Translation: AAC41917.1 .
AY325910 Genomic DNA. Translation: AAP78485.1 .
AK313109 mRNA. Translation: BAG35931.1 .
CH471114 Genomic DNA. Translation: EAW95519.1 .
BC009581 mRNA. Translation: AAH09581.1 .
BC059170 mRNA. Translation: AAH59170.1 .
CCDSi CCDS10936.1.
PIRi A47287.
RefSeqi NP_002144.1. NM_002153.2.
UniGenei Hs.162795.

3D structure databases

ProteinModelPortali P37059.
SMRi P37059. Positions 83-316.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9606.ENSP00000199936.

Chemistry

BindingDBi P37059.
ChEMBLi CHEMBL2789.
DrugBanki DB00157. NADH.

PTM databases

PhosphoSitei P37059.

Polymorphism databases

DMDMi 544152.

Proteomic databases

MaxQBi P37059.
PaxDbi P37059.
PeptideAtlasi P37059.
PRIDEi P37059.

Protocols and materials databases

DNASUi 3294.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000199936 ; ENSP00000199936 ; ENSG00000086696 .
GeneIDi 3294.
KEGGi hsa:3294.
UCSCi uc002fgv.3. human.

Organism-specific databases

CTDi 3294.
GeneCardsi GC16P082068.
HGNCi HGNC:5211. HSD17B2.
HPAi HPA021826.
MIMi 109685. gene.
neXtProti NX_P37059.
PharmGKBi PA29479.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG1028.
HOVERGENi HBG005482.
InParanoidi P37059.
KOi K13368.
OMAi QEDYGQD.
PhylomeDBi P37059.
TreeFami TF325617.

Miscellaneous databases

GeneWikii HSD17B2.
GenomeRNAii 3294.
NextBioi 13067.
PROi P37059.
SOURCEi Search...

Gene expression databases

ArrayExpressi P37059.
Bgeei P37059.
CleanExi HS_HSD17B2.
Genevestigatori P37059.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view ]
Pfami PF00106. adh_short. 1 hit.
[Graphical view ]
PRINTSi PR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEi PS00061. ADH_SHORT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Expression cloning and characterization of human 17 beta-hydroxysteroid dehydrogenase type 2, a microsomal enzyme possessing 20 alpha-hydroxysteroid dehydrogenase activity."
    Wu L., Einstein M., Geissler W.M., Chan H.K., Elliston K.O., Andersson S.
    J. Biol. Chem. 268:12964-12969(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES.
    Tissue: Prostate.
  2. "The human type II 17 beta-hydroxysteroid dehydrogenase gene encodes two alternatively spliced mRNA species."
    Labrie Y., Durocher F., Lachance Y., Turgeon C., Simard J., Labrie C., Labrie F.
    DNA Cell Biol. 14:849-862(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. NIEHS SNPs program
    Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT THR-121.
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Colon.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Bone marrow.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDHB2_HUMAN
AccessioniPrimary (citable) accession number: P37059
Secondary accession number(s): B2R7T4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: July 9, 2014
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi