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P37059 (DHB2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 134. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Estradiol 17-beta-dehydrogenase 2

EC=1.1.1.62
Alternative name(s):
17-beta-hydroxysteroid dehydrogenase type 2
Short name=17-beta-HSD 2
20 alpha-hydroxysteroid dehydrogenase
Short name=20-alpha-HSD
E2DH
Microsomal 17-beta-hydroxysteroid dehydrogenase
Testosterone 17-beta-dehydrogenase
EC=1.1.1.239
Gene names
Name:HSD17B2
Synonyms:EDH17B2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length387 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH.

Catalytic activity

17-beta-estradiol + NAD(P)+ = estrone + NAD(P)H.

Testosterone + NAD+ = androstenedione + NADH.

Subcellular location

Membrane; Single-pass type II membrane protein Potential.

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Biophysicochemical properties

Kinetic parameters:

KM=0.21 µM for estradiol Ref.1

KM=0.39 µM for testosterone

KM=0.31 µM for dihydrotestosterone

KM=0.71 µM for 20-alpha-dihydroprogesterone

KM=0.78 µM for estrone

KM=2.63 µM for androstenedione

Vmax=38 nmol/min/mg enzyme with estradiol as substrate

Vmax=45 nmol/min/mg enzyme with testosterone as substrate

Vmax=38 nmol/min/mg enzyme with dihydrotestosterone as substrate

Vmax=5.6 nmol/min/mg enzyme with 20-alpha-dihydroprogesterone as substrate

Vmax=6.6 nmol/min/mg enzyme with estrone as substrate

Vmax=11.5 nmol/min/mg enzyme with androstenedione as substrate

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 387387Estradiol 17-beta-dehydrogenase 2
PRO_0000054570

Regions

Transmembrane4 – 2421Helical; Signal-anchor for type II membrane protein; Potential
Nucleotide binding82 – 11130NAD By similarity

Sites

Active site2321 By similarity
Binding site2191Substrate By similarity

Natural variations

Natural variant1211A → T. Ref.3
Corresponds to variant rs8191136 [ dbSNP | Ensembl ].
VAR_018852

Sequences

Sequence LengthMass (Da)Tools
P37059 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: EDE5606EA041042B

FASTA38742,785
        10         20         30         40         50         60 
MSTFFSDTAW ICLAVPTVLC GTVFCKYKKS SGQLWSWMVC LAGLCAVCLL ILSPFWGLIL 

        70         80         90        100        110        120 
FSVSCFLMYT YLSGQELLPV DQKAVLVTGG DCGLGHALCK YLDELGFTVF AGVLNENGPG 

       130        140        150        160        170        180 
AEELRRTCSP RLSVLQMDIT KPVQIKDAYS KVAAMLQDRG LWAVINNAGV LGFPTDGELL 

       190        200        210        220        230        240 
LMTDYKQCMA VNFFGTVEVT KTFLPLLRKS KGRLVNVSSM GGGAPMERLA SYGSSKAAVT 

       250        260        270        280        290        300 
MFSSVMRLEL SKWGIKVASI QPGGFLTNIA GTSDKWEKLE KDILDHLPAE VQEDYGQDYI 

       310        320        330        340        350        360 
LAQRNFLLLI NSLASKDFSP VLRDIQHAIL AKSPFAYYTP GKGAYLWICL AHYLPIGIYD 

       370        380 
YFAKRHFGQD KPMPRALRMP NYKKKAT 

« Hide

References

« Hide 'large scale' references
[1]"Expression cloning and characterization of human 17 beta-hydroxysteroid dehydrogenase type 2, a microsomal enzyme possessing 20 alpha-hydroxysteroid dehydrogenase activity."
Wu L., Einstein M., Geissler W.M., Chan H.K., Elliston K.O., Andersson S.
J. Biol. Chem. 268:12964-12969(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES.
Tissue: Prostate.
[2]"The human type II 17 beta-hydroxysteroid dehydrogenase gene encodes two alternatively spliced mRNA species."
Labrie Y., Durocher F., Lachance Y., Turgeon C., Simard J., Labrie C., Labrie F.
DNA Cell Biol. 14:849-862(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]NIEHS SNPs program
Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT THR-121.
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Colon.
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Bone marrow.
[7]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Web resources

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L11708 mRNA. Translation: AAA03562.1.
L40802 expand/collapse EMBL AC list , L40796, L40797, L40798, L40801 Genomic DNA. Translation: AAC41917.1.
AY325910 Genomic DNA. Translation: AAP78485.1.
AK313109 mRNA. Translation: BAG35931.1.
CH471114 Genomic DNA. Translation: EAW95519.1.
BC009581 mRNA. Translation: AAH09581.1.
BC059170 mRNA. Translation: AAH59170.1.
PIRA47287.
RefSeqNP_002144.1. NM_002153.2.
UniGeneHs.162795.

3D structure databases

ProteinModelPortalP37059.
SMRP37059. Positions 81-354.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000199936.

Chemistry

BindingDBP37059.
ChEMBLCHEMBL2789.
DrugBankDB00157. NADH.

PTM databases

PhosphoSiteP37059.

Polymorphism databases

DMDM544152.

Proteomic databases

PaxDbP37059.
PeptideAtlasP37059.
PRIDEP37059.

Protocols and materials databases

DNASU3294.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000199936; ENSP00000199936; ENSG00000086696.
GeneID3294.
KEGGhsa:3294.
UCSCuc002fgv.3. human.

Organism-specific databases

CTD3294.
GeneCardsGC16P082068.
HGNCHGNC:5211. HSD17B2.
HPAHPA021826.
MIM109685. gene.
neXtProtNX_P37059.
PharmGKBPA29479.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1028.
HOVERGENHBG005482.
InParanoidP37059.
KOK13368.
OMAQEDYGQD.
PhylomeDBP37059.
TreeFamTF325617.

Gene expression databases

ArrayExpressP37059.
BgeeP37059.
CleanExHS_HSD17B2.
GenevestigatorP37059.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiHSD17B2.
GenomeRNAi3294.
NextBio13067.
PROP37059.
SOURCESearch...

Entry information

Entry nameDHB2_HUMAN
AccessionPrimary (citable) accession number: P37059
Secondary accession number(s): B2R7T4
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: March 19, 2014
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 16

Human chromosome 16: entries, gene names and cross-references to MIM