Reviewed,
UniProtKB/Swiss-Prot P37059 (DHB2_HUMAN)
Last modified
November 3, 2009.
Version 90.
History...
Clusters with 100%,
90%,
50% identity |
Documents (5) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: Estradiol 17-beta-dehydrogenase 2 EC=1.1.1.62 Alternative name(s): Testosterone 17-beta-dehydrogenase EC=1.1.1.63 Microsomal 17-beta-hydroxysteroid dehydrogenase 17-beta-HSD 2 20 alpha-hydroxysteroid dehydrogenase Short name=20-alpha-HSD E2DH | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 387 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH. |
| Catalytic activity | Estradiol-17-beta + NAD(P)+ = estrone + NAD(P)H. Testosterone + NAD+ = androst-4-ene-3,17-dione + NADH. |
| Subcellular location | Membrane; Single-pass type II membrane protein Potential. |
| Sequence similarities | Belongs to the short-chain dehydrogenases/reductases (SDR) family. |
| Biophysicochemical properties | Kinetic parameters: KM=0.21 µM for estradiol KM=0.39 µM for testosterone KM=0.31 µM for dihydrotestosterone KM=0.71 µM for 20-alpha-dihydroprogesterone KM=0.78 µM for estrone KM=2.63 µM for androstenedione Vmax=38 nmol/min/mg enzyme with estradiol as substrate Vmax=45 nmol/min/mg enzyme with testosterone as substrate Vmax=38 nmol/min/mg enzyme with dihydrotestosterone as substrate Vmax=5.6 nmol/min/mg enzyme with 20-alpha-dihydroprogesterone as substrate Vmax=6.6 nmol/min/mg enzyme with estrone as substrate Vmax=11.5 nmol/min/mg enzyme with androstenedione as substrate |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 387 | 387 | Estradiol 17-beta-dehydrogenase 2 | PRO_0000054570 | |||||
Regions | |||||||||
| Transmembrane | 4 – 24 | 21 | Signal-anchor for type II membrane protein Potential | ||||||
| Nucleotide binding | 82 – 111 | 30 | NAD By similarity | ||||||
Sites | |||||||||
| Active site | 232 | 1 | By similarity | ||||||
| Binding site | 219 | 1 | Substrate By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 273 | 1 | Phosphoserine Ref.7 | ||||||
Natural variations | |||||||||
| Natural variant | 121 | 1 | A → T: dbSNP rs8191136. Ref.3 | VAR_018852 | |||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "Expression cloning and characterization of human 17 beta-hydroxysteroid dehydrogenase type 2, a microsomal enzyme possessing 20 alpha-hydroxysteroid dehydrogenase activity." Wu L., Einstein M., Geissler W.M., Chan H.K., Elliston K.O., Andersson S. J. Biol. Chem. 268:12964-12969(1993) [PubMed: 8099587] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES. Tissue: Prostate. |
| [2] | "The human type II 17 beta-hydroxysteroid dehydrogenase gene encodes two alternatively spliced mRNA species." Labrie Y., Durocher F., Lachance Y., Turgeon C., Simard J., Labrie C., Labrie F. DNA Cell Biol. 14:849-862(1995) [PubMed: 7546291] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | NIEHS SNPs program Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT THR-121. |
| [4] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon. |
| [5] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [6] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone marrow. |
| [7] | "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-273, MASS SPECTROMETRY. Tissue: Epithelium. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| L11708 mRNA. Translation: AAA03562.1. L40802 L40801 Genomic DNA. Translation: AAC41917.1. AY325910 Genomic DNA. Translation: AAP78485.1. AK313109 mRNA. Translation: BAG35931.1. CH471114 Genomic DNA. Translation: EAW95519.1. BC009581 mRNA. Translation: AAH09581.1. BC059170 mRNA. Translation: AAH59170.1. | |
| IPI | IPI00019872. |
| PIR | A47287. |
| RefSeq | NP_002144.1. |
| UniGene | Hs.162795 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P37059. |
Proteomic databases | |
| PeptideAtlas | P37059. |
| PRIDE | P37059. |
Genome annotation databases | |
| Ensembl | ENST00000199936; ENSP00000199936; ENSG00000086696; Homo sapiens. [Genome view] |
| GeneID | 3294. |
| KEGG | hsa:3294. |
| UCSC | uc002fgv.1. human. |
Organism-specific databases | |
| CTD | 3294. |
| GeneCards | GC16P080626. |
| H-InvDB | HIX0013279. |
| HGNC | HGNC:5211. HSD17B2. |
| MIM | 109685. gene. |
| PharmGKB | PA29479. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | P37059. |
| HOVERGEN | P37059. |
| OMA | QEDYGQD. |
Enzyme and pathway databases | |
| BRENDA | 1.1.1.62. 247. 1.1.1.63. 247. |
Gene expression databases | |
| ArrayExpress | P37059. |
| Bgee | P37059. |
| CleanEx | HS_HSD17B2. |
| Genevestigator | P37059. |
| GermOnline | ENSG00000086696. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| PANTHER | PTHR19410. ADH_short_C2. 1 hit. |
| Pfam | PF00106. adh_short. 1 hit. [Graphical view] |
| PRINTS | PR00081. GDHRDH. PR00080. SDRFAMILY. |
| PROSITE | PS00061. ADH_SHORT. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| DrugBank | DB00157. NADH. |
| NextBio | 13067. |
| SOURCE | Search... |
Entry information
| Entry name | DHB2_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P37059 Secondary accession number(s): B2R7T4 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 16 Human chromosome 16: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


