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P37059

- DHB2_HUMAN

UniProt

P37059 - DHB2_HUMAN

Protein

Estradiol 17-beta-dehydrogenase 2

Gene

HSD17B2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 138 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH.

    Catalytic activityi

    17-beta-estradiol + NAD(P)+ = estrone + NAD(P)H.
    Testosterone + NAD+ = androstenedione + NADH.

    Kineticsi

    1. KM=0.21 µM for estradiol1 Publication
    2. KM=0.39 µM for testosterone1 Publication
    3. KM=0.31 µM for dihydrotestosterone1 Publication
    4. KM=0.71 µM for 20-alpha-dihydroprogesterone1 Publication
    5. KM=0.78 µM for estrone1 Publication
    6. KM=2.63 µM for androstenedione1 Publication

    Vmax=38 nmol/min/mg enzyme with estradiol as substrate1 Publication

    Vmax=45 nmol/min/mg enzyme with testosterone as substrate1 Publication

    Vmax=38 nmol/min/mg enzyme with dihydrotestosterone as substrate1 Publication

    Vmax=5.6 nmol/min/mg enzyme with 20-alpha-dihydroprogesterone as substrate1 Publication

    Vmax=6.6 nmol/min/mg enzyme with estrone as substrate1 Publication

    Vmax=11.5 nmol/min/mg enzyme with androstenedione as substrate1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei219 – 2191SubstrateBy similarity
    Active sitei232 – 2321By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi82 – 11130NADBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. 17-alpha,20-alpha-dihydroxypregn-4-en-3-one dehydrogenase activity Source: BHF-UCL
    2. estradiol 17-beta-dehydrogenase activity Source: UniProtKB
    3. testosterone dehydrogenase (NAD+) activity Source: UniProtKB

    GO - Biological processi

    1. in utero embryonic development Source: Ensembl
    2. placenta development Source: Ensembl
    3. response to retinoic acid Source: BHF-UCL
    4. steroid biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis

    Keywords - Ligandi

    NAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Estradiol 17-beta-dehydrogenase 2 (EC:1.1.1.62)
    Alternative name(s):
    17-beta-hydroxysteroid dehydrogenase type 2
    Short name:
    17-beta-HSD 2
    20 alpha-hydroxysteroid dehydrogenase
    Short name:
    20-alpha-HSD
    E2DH
    Microsomal 17-beta-hydroxysteroid dehydrogenase
    Testosterone 17-beta-dehydrogenase (EC:1.1.1.239)
    Gene namesi
    Name:HSD17B2
    Synonyms:EDH17B2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 16

    Organism-specific databases

    HGNCiHGNC:5211. HSD17B2.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: ProtInc
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA29479.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 387387Estradiol 17-beta-dehydrogenase 2PRO_0000054570Add
    BLAST

    Proteomic databases

    MaxQBiP37059.
    PaxDbiP37059.
    PeptideAtlasiP37059.
    PRIDEiP37059.

    PTM databases

    PhosphoSiteiP37059.

    Expressioni

    Gene expression databases

    ArrayExpressiP37059.
    BgeeiP37059.
    CleanExiHS_HSD17B2.
    GenevestigatoriP37059.

    Organism-specific databases

    HPAiHPA021826.

    Interactioni

    Protein-protein interaction databases

    STRINGi9606.ENSP00000199936.

    Structurei

    3D structure databases

    ProteinModelPortaliP37059.
    SMRiP37059. Positions 83-316.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei4 – 2421Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1028.
    HOVERGENiHBG005482.
    InParanoidiP37059.
    KOiK13368.
    OMAiQEDYGQD.
    PhylomeDBiP37059.
    TreeFamiTF325617.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view]
    PfamiPF00106. adh_short. 1 hit.
    [Graphical view]
    PRINTSiPR00081. GDHRDH.
    PR00080. SDRFAMILY.
    PROSITEiPS00061. ADH_SHORT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P37059-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTFFSDTAW ICLAVPTVLC GTVFCKYKKS SGQLWSWMVC LAGLCAVCLL    50
    ILSPFWGLIL FSVSCFLMYT YLSGQELLPV DQKAVLVTGG DCGLGHALCK 100
    YLDELGFTVF AGVLNENGPG AEELRRTCSP RLSVLQMDIT KPVQIKDAYS 150
    KVAAMLQDRG LWAVINNAGV LGFPTDGELL LMTDYKQCMA VNFFGTVEVT 200
    KTFLPLLRKS KGRLVNVSSM GGGAPMERLA SYGSSKAAVT MFSSVMRLEL 250
    SKWGIKVASI QPGGFLTNIA GTSDKWEKLE KDILDHLPAE VQEDYGQDYI 300
    LAQRNFLLLI NSLASKDFSP VLRDIQHAIL AKSPFAYYTP GKGAYLWICL 350
    AHYLPIGIYD YFAKRHFGQD KPMPRALRMP NYKKKAT 387
    Length:387
    Mass (Da):42,785
    Last modified:June 1, 1994 - v1
    Checksum:iEDE5606EA041042B
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti121 – 1211A → T.1 Publication
    Corresponds to variant rs8191136 [ dbSNP | Ensembl ].
    VAR_018852

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L11708 mRNA. Translation: AAA03562.1.
    L40802
    , L40796, L40797, L40798, L40801 Genomic DNA. Translation: AAC41917.1.
    AY325910 Genomic DNA. Translation: AAP78485.1.
    AK313109 mRNA. Translation: BAG35931.1.
    CH471114 Genomic DNA. Translation: EAW95519.1.
    BC009581 mRNA. Translation: AAH09581.1.
    BC059170 mRNA. Translation: AAH59170.1.
    CCDSiCCDS10936.1.
    PIRiA47287.
    RefSeqiNP_002144.1. NM_002153.2.
    UniGeneiHs.162795.

    Genome annotation databases

    EnsembliENST00000199936; ENSP00000199936; ENSG00000086696.
    GeneIDi3294.
    KEGGihsa:3294.
    UCSCiuc002fgv.3. human.

    Polymorphism databases

    DMDMi544152.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    NIEHS-SNPs

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L11708 mRNA. Translation: AAA03562.1 .
    L40802
    , L40796 , L40797 , L40798 , L40801 Genomic DNA. Translation: AAC41917.1 .
    AY325910 Genomic DNA. Translation: AAP78485.1 .
    AK313109 mRNA. Translation: BAG35931.1 .
    CH471114 Genomic DNA. Translation: EAW95519.1 .
    BC009581 mRNA. Translation: AAH09581.1 .
    BC059170 mRNA. Translation: AAH59170.1 .
    CCDSi CCDS10936.1.
    PIRi A47287.
    RefSeqi NP_002144.1. NM_002153.2.
    UniGenei Hs.162795.

    3D structure databases

    ProteinModelPortali P37059.
    SMRi P37059. Positions 83-316.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000199936.

    Chemistry

    BindingDBi P37059.
    ChEMBLi CHEMBL2789.
    DrugBanki DB00157. NADH.

    PTM databases

    PhosphoSitei P37059.

    Polymorphism databases

    DMDMi 544152.

    Proteomic databases

    MaxQBi P37059.
    PaxDbi P37059.
    PeptideAtlasi P37059.
    PRIDEi P37059.

    Protocols and materials databases

    DNASUi 3294.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000199936 ; ENSP00000199936 ; ENSG00000086696 .
    GeneIDi 3294.
    KEGGi hsa:3294.
    UCSCi uc002fgv.3. human.

    Organism-specific databases

    CTDi 3294.
    GeneCardsi GC16P082068.
    HGNCi HGNC:5211. HSD17B2.
    HPAi HPA021826.
    MIMi 109685. gene.
    neXtProti NX_P37059.
    PharmGKBi PA29479.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1028.
    HOVERGENi HBG005482.
    InParanoidi P37059.
    KOi K13368.
    OMAi QEDYGQD.
    PhylomeDBi P37059.
    TreeFami TF325617.

    Miscellaneous databases

    GeneWikii HSD17B2.
    GenomeRNAii 3294.
    NextBioi 13067.
    PROi P37059.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P37059.
    Bgeei P37059.
    CleanExi HS_HSD17B2.
    Genevestigatori P37059.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view ]
    Pfami PF00106. adh_short. 1 hit.
    [Graphical view ]
    PRINTSi PR00081. GDHRDH.
    PR00080. SDRFAMILY.
    PROSITEi PS00061. ADH_SHORT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression cloning and characterization of human 17 beta-hydroxysteroid dehydrogenase type 2, a microsomal enzyme possessing 20 alpha-hydroxysteroid dehydrogenase activity."
      Wu L., Einstein M., Geissler W.M., Chan H.K., Elliston K.O., Andersson S.
      J. Biol. Chem. 268:12964-12969(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES.
      Tissue: Prostate.
    2. "The human type II 17 beta-hydroxysteroid dehydrogenase gene encodes two alternatively spliced mRNA species."
      Labrie Y., Durocher F., Lachance Y., Turgeon C., Simard J., Labrie C., Labrie F.
      DNA Cell Biol. 14:849-862(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. NIEHS SNPs program
      Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT THR-121.
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Colon.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Bone marrow.
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiDHB2_HUMAN
    AccessioniPrimary (citable) accession number: P37059
    Secondary accession number(s): B2R7T4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 138 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 16
      Human chromosome 16: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3