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Protein

Formyltetrahydrofolate deformylase

Gene

purU

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of 10-formyltetrahydrofolate (formyl-FH4) to formate and tetrahydrofolate (FH4). Provides the major source of formate for the PurT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) during aerobic growth. Has a role in regulating the one-carbon pool.UniRule annotation1 Publication

Catalytic activityi

10-formyltetrahydrofolate + H2O = formate + tetrahydrofolate.UniRule annotation1 Publication

Enzyme regulationi

Activated by methionine, inhibited by glycine.1 Publication

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes formate from 10-formyl-5,6,7,8-tetrahydrofolate.UniRule annotation1 Publication
Proteins known to be involved in this subpathway in this organism are:
  1. Formyltetrahydrofolate deformylase (purU)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes formate from 10-formyl-5,6,7,8-tetrahydrofolate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei225UniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  • 10-formyltetrahydrofolate biosynthetic process Source: EcoCyc
  • one-carbon metabolic process Source: EcoCyc
  • purine nucleotide biosynthetic process Source: CACAO
  • purine ribonucleotide biosynthetic process Source: EcoCyc

Keywordsi

Molecular functionHydrolase
Biological processOne-carbon metabolism, Purine biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:FORMYLTHFDEFORMYL-MONOMER
MetaCyc:FORMYLTHFDEFORMYL-MONOMER
UniPathwayiUPA00074; UER00170

Names & Taxonomyi

Protein namesi
Recommended name:
Formyltetrahydrofolate deformylaseUniRule annotation (EC:3.5.1.10UniRule annotation)
Alternative name(s):
Formyl-FH(4) hydrolaseUniRule annotation
Gene namesi
Name:purUUniRule annotation
Synonyms:tgs, ychI
Ordered Locus Names:b1232, JW1220
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11819 purU

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000749611 – 280Formyltetrahydrofolate deformylaseAdd BLAST280

Proteomic databases

EPDiP37051
PaxDbiP37051
PRIDEiP37051

Interactioni

Subunit structurei

Homohexamer.1 Publication

Protein-protein interaction databases

BioGridi4261924, 11 interactors
IntActiP37051, 3 interactors
STRINGi316385.ECDH10B_1292

Structurei

3D structure databases

ProteinModelPortaliP37051
SMRiP37051
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 86ACTUniRule annotationAdd BLAST79

Sequence similaritiesi

Belongs to the PurU family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CIY Bacteria
COG0788 LUCA
HOGENOMiHOG000033576
InParanoidiP37051
KOiK01433
OMAiHADHHTD
PhylomeDBiP37051

Family and domain databases

HAMAPiMF_01927 PurU, 1 hit
InterProiView protein in InterPro
IPR002912 ACT_dom
IPR002376 Formyl_transf_N
IPR036477 Formyl_transf_N_sf
IPR004810 PurU
PANTHERiPTHR42706 PTHR42706, 1 hit
PfamiView protein in Pfam
PF01842 ACT, 1 hit
PF00551 Formyl_trans_N, 1 hit
PRINTSiPR01575 FFH4HYDRLASE
SUPFAMiSSF53328 SSF53328, 1 hit
TIGRFAMsiTIGR00655 PurU, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 1 hit

Sequencei

Sequence statusi: Complete.

P37051-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHSLQRKVLR TICPDQKGLI ARITNICYKH ELNIVQNNEF VDHRTGRFFM
60 70 80 90 100
RTELEGIFND STLLADLDSA LPEGSVRELN PAGRRRIVIL VTKEAHCLGD
110 120 130 140 150
LLMKANYGGL DVEIAAVIGN HDTLRSLVER FDIPFELVSH EGLTRNEHDQ
160 170 180 190 200
KMADAIDAYQ PDYVVLAKYM RVLTPEFVAR FPNKIINIHH SFLPAFIGAR
210 220 230 240 250
PYHQAYERGV KIIGATAHYV NDNLDEGPII MQDVIHVDHT YTAEDMMRAG
260 270 280
RDVEKNVLSR ALYKVLAQRV FVYGNRTIIL
Length:280
Mass (Da):31,935
Last modified:June 1, 1994 - v1
Checksum:i5667406D2727A2C2
GO

Sequence cautioni

The sequence AAA16860 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20251 Unassigned DNA Translation: AAC36846.1
M64675 Unassigned DNA Translation: AAA16860.1 Different initiation.
U00096 Genomic DNA Translation: AAC74314.1
AP009048 Genomic DNA Translation: BAA36100.1
PIRiC36871
RefSeqiNP_415748.1, NC_000913.3
WP_000555857.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74314; AAC74314; b1232
BAA36100; BAA36100; BAA36100
GeneIDi945827
KEGGiecj:JW1220
eco:b1232
PATRICifig|1411691.4.peg.1053

Similar proteinsi

Entry informationi

Entry nameiPURU_ECOLI
AccessioniPrimary (citable) accession number: P37051
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: March 28, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health