P37051 (PURU_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formyltetrahydrofolate deformylase
EC=3.5.1.10

Alternative name(s):
Formyl-FH(4) hydrolase

Gene names

Name:	purU
Synonyms:	tgs, ychI
Ordered Locus Names:	b1232, JW1220

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	280 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Produces formate from formyl-tetrahydrofolate. Provides the major source of formate for the purT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) during aerobic growth. Has a role in regulating the one-carbon pool.
Catalytic activity	10-formyltetrahydrofolate + H2O = formate + tetrahydrofolate.
Enzyme regulation	Activated by methionine, inhibited by glycine.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; formate from 10-formyl-5,6,7,8-tetrahydrofolate: step 1/1.
Subunit structure	Homohexamer.
Sequence similarities	Belongs to the purU family. Contains 1 ACT domain.
Sequence caution	The sequence AAA16860.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	One-carbon metabolism Purine biosynthesis
Molecular function	Hydrolase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro 10-formyltetrahydrofolate biosynthetic process Traceable author statement. Source: EcoCyc one-carbon metabolic process Traceable author statement. Source: EcoCyc
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro formyltetrahydrofolate deformylase activity Inferred from direct assay Ref.6. Source: EcoCyc hydroxymethyl-, formyl- and related transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 280	280	Formyltetrahydrofolate deformylase		PRO_0000074961
Regions
	Domain	7 – 79	73	ACT
Sites
	Active site	225	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P37051 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 5667406D2727A2C2	FASTA	280	31,935
	10 20 30 40 50 60 MHSLQRKVLR TICPDQKGLI ARITNICYKH ELNIVQNNEF VDHRTGRFFM RTELEGIFND 70 80 90 100 110 120 STLLADLDSA LPEGSVRELN PAGRRRIVIL VTKEAHCLGD LLMKANYGGL DVEIAAVIGN 130 140 150 160 170 180 HDTLRSLVER FDIPFELVSH EGLTRNEHDQ KMADAIDAYQ PDYVVLAKYM RVLTPEFVAR 190 200 210 220 230 240 FPNKIINIHH SFLPAFIGAR PYHQAYERGV KIIGATAHYV NDNLDEGPII MQDVIHVDHT 250 260 270 280 YTAEDMMRAG RDVEKNVLSR ALYKVLAQRV FVYGNRTIIL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"purU, a source of formate for purT-dependent phosphoribosyl-N-formylglycinamide synthesis." Nagy P.L., McCorkle G., Zalkin H. J. Bacteriol. 175:7066-7073(1993) [PubMed: 8226647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-6. Strain: K12.
[2]	"Organization and functions of genes in the upstream region of tyrT of Escherichia coli: phenotypes of mutants with partial deletion of a new gene (tgs)." Boesl M., Kersten H. J. Bacteriol. 176:221-231(1994) [PubMed: 8282700] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Formyltetrahydrofolate hydrolase, a regulatory enzyme that functions to balance pools of tetrahydrofolate and one-carbon tetrahydrofolate adducts in Escherichia coli." Nagy P.L., Marolewski A., Benkovic S.J., Zalkin H. J. Bacteriol. 177:1292-1298(1995) [PubMed: 7868604] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L20251 Unassigned DNA. Translation: AAC36846.1. M64675 Unassigned DNA. Translation: AAA16860.1. Different initiation. U00096 Genomic DNA. Translation: AAC74314.1. AP009048 Genomic DNA. Translation: BAA36100.1.
PIR	C36871.
RefSeq	NP_415748.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P37051.
SMR	P37051. Positions 2-280.
ModBase	Search...
Protein-protein interaction databases
IntAct	P37051. 3 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000000642; EBESCP00000000642; EBESCG00000000535. EBESCT00000017337; EBESCP00000016628; EBESCG00000016393.
GeneID	945827.
GenomeReviews	Gene locus JW1220 in contig AP009048_GR. Gene locus b1232 in contig U00096_GR.
KEGG	ecj:JW1220. eco:b1232.
PATRIC	32117720. VBIEscCol129921_1280.
Organism-specific databases
EchoBASE	EB1766.
EcoGene	EG11819. purU.
Phylogenomic databases
eggNOG	COG0788.
GeneTree	EBGT00050000009381.
HOGENOM	HBG286016.
OMA	HEGLTRD.
PhylomeDB	P37051.
ProtClustDB	PRK06027.
Enzyme and pathway databases
BioCyc	EcoCyc:FORMYLTHFDEFORMYL-MONOMER. MetaCyc:FORMYLTHFDEFORMYL-MONOMER.
Gene expression databases
Genevestigator	P37051.
Family and domain databases
InterPro	IPR002912. ACT-bd. IPR002376. Formyl_transf_N. IPR004810. PurU. [Graphical view]
Gene3D	G3DSA:3.40.50.170. Formyl_transf_N. 1 hit.
KO	K01433.
PANTHER	PTHR10520:SF7. PTHR10520:SF7. 1 hit.
Pfam	PF01842. ACT. 1 hit. PF00551. Formyl_trans_N. 1 hit. [Graphical view]
PIRSF	PIRSF036480. FormyFH4_hydr. 1 hit.
PRINTS	PR01575. FFH4HYDRLASE.
SUPFAM	SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00655. PurU. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURU_ECOLI

Accession

Primary (citable) accession number: P37051

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	January 25, 2012
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program