P37049 (YAEI_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphodiesterase YaeI
EC=3.1.4.-

Gene names

Name:	yaeI
Ordered Locus Names:	b0164, JW5014

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	270 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Shows phosphodiesterase activity, hydrolyzing phosphodiester bond in the artificial chromogenic substrate bis-p-nitrophenyl phosphate (bis-pNPP). Ref.5
Cofactor	Binds 2 divalent metal cations By similarity.
Sequence similarities	Belongs to the metallophosphoesterase superfamily.

Ontologies

Keywords
Ligand	Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 270	270	Phosphodiesterase YaeI		PRO_0000172849
Sites
	Metal binding	56	1	Divalent metal cation 1 By similarity
	Metal binding	58	1	Divalent metal cation 1 By similarity
	Metal binding	88	1	Divalent metal cation 1 By similarity
	Metal binding	88	1	Divalent metal cation 2 By similarity
	Metal binding	120	1	Divalent metal cation 2 By similarity
	Metal binding	209	1	Divalent metal cation 2 By similarity
	Metal binding	211	1	Divalent metal cation 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P37049 [UniParc]. Last modified April 27, 2001. Version 2. Checksum: 6A075D0C09E5CAE7	FASTA	270	29,863
	10 20 30 40 50 60 MISRRRFLQA TAATIATSSG FGYMHYCEPG WFELIRHRLA FFKDNAAPFK ILFLADLHYS 70 80 90 100 110 120 RFVPLSLISD AIALGIEQKP DLILLGGDYV LFDMSLNFSA FSDVLSPLAE CAPTFACFGN 130 140 150 160 170 180 HDRPVGTEKN HLIGETLKSA GITVLFNQAT VIATPNRQFE LVGTGDLWAG QCKPPPASEA 190 200 210 220 230 240 NLPRLVLAHN PDSKEVMRDE PWDLMLCGHT HGGQLRVPLV GEPFAPVEDK RYVAGLNAFG 250 260 270 ERHIYTTRGV GSLYGLRLNC RPEVTMLELV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region." Fujita N., Mori H., Yura T., Ishihama A. Nucleic Acids Res. 22:1637-1639(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Enzyme genomics: application of general enzymatic screens to discover new enzymes." Kuznetsova E., Proudfoot M., Sanders S.A., Reinking J., Savchenko A., Arrowsmith C.H., Edwards A.M., Yakunin A.F. FEMS Microbiol. Rev. 29:263-279(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U70214 Genomic DNA. Translation: AAB08594.1. U00096 Genomic DNA. Translation: AAC73275.2. AP009048 Genomic DNA. Translation: BAB96741.2.
PIR	D64740.
RefSeq	NP_414706.2. NC_000913.2. YP_488467.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P37049.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b0164.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73275; AAC73275; b0164. BAB96741; BAB96741; BAB96741.
GeneID	12931652. 945002.
KEGG	ecj:Y75_p0161. eco:b0164.
PATRIC	32115437. VBIEscCol129921_0170.
Organism-specific databases
EchoBASE	EB2241.
EcoGene	EG12337. yaeI.
Phylogenomic databases
eggNOG	COG1408.
HOGENOM	HOG000260481.
KO	K07098.
OMA	DALEANQ.
ProtClustDB	PRK11340.
Enzyme and pathway databases
BioCyc	EcoCyc:EG12337-MONOMER. ECOL316407:JW5014-MONOMER.
BRENDA	3.1.4.1. 2026.
Gene expression databases
Genevestigator	P37049.
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YAEI_ECOLI

Accession

Primary (citable) accession number: P37049
Secondary accession number(s): P75666, Q8KJP9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	April 27, 2001
Last modified:	May 1, 2013
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program