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P37045

- TOG4B_AGEAP

UniProt

P37045 - TOG4B_AGEAP

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Protein

Omega-agatoxin-Aa4b

Gene
N/A
Organism
Agelenopsis aperta (North American funnel-web spider) (Agelenopsis gertschi)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Antagonist of voltage-gated Cav2.1/CACNA1A (P-type) calcium channels. Paralyzes insect by blocking neuromuscular transmission.1 Publication

GO - Molecular functioni

  1. sodium channel inhibitor activity Source: InterPro

GO - Biological processi

  1. pathogenesis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel impairing toxin, Ion channel impairing toxin, Neurotoxin, Presynaptic neurotoxin, Toxin, Voltage-gated calcium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Omega-agatoxin-Aa4b
Short name:
Omega-AGTX-Aa4b
Alternative name(s):
Omega-agatoxin IVB
Short name:
Omega-Aga-IVB
Omega-agatoxin IVC
Short name:
Omega-Aga-IVC
Omega-agatoxin tsukuba
Short name:
Omega-Aga-TK
Short name:
Omega-agatoxin-TK
Omega-agatoxin-4B
OrganismiAgelenopsis aperta (North American funnel-web spider) (Agelenopsis gertschi)
Taxonomic identifieri6908 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeAraneomorphaeEntelegynaeAgelenidaeAgelenopsis

Organism-specific databases

ArachnoServeriAS000183. omega-agatoxin-Aa4b.

Subcellular locationi

GO - Cellular componenti

  1. other organism presynaptic membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020 Reviewed predictionAdd
BLAST
Propeptidei21 – 3515 By similarityPRO_0000035490Add
BLAST
Chaini36 – 8348Omega-agatoxin-Aa4bPRO_0000035491Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi39 ↔ 554 Publications
Disulfide bondi47 ↔ 604 Publications
Disulfide bondi54 ↔ 714 Publications
Disulfide bondi62 ↔ 694 Publications
Modified residuei81 – 811D-serine (Ser)3 Publications

Post-translational modificationi

The toxin with D-Ser (named omega-aga IVC) is 80-90 fold more potent than that with L-Ser (omega-aga IVB) against Cav2.1/CACNA1A (P-type) channels in rat cerebellar Purkinje neurons and is more resistant to proteases. The epimerization is done by the venom peptide isomerase heterodimer.

Keywords - PTMi

D-amino acid, Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

1
83
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi49 – 513
Turni55 – 573
Beta strandi60 – 623
Beta strandi64 – 718
Turni76 – 794

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AGGNMR-A36-83[»]
1OMANMR-A36-83[»]
1OMBNMR-A36-83[»]
ProteinModelPortaliP37045.
SMRiP37045. Positions 36-83.

Miscellaneous databases

EvolutionaryTraceiP37045.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Sequence similaritiesi

Keywords - Domaini

Knottin, Signal

Family and domain databases

Gene3Di4.10.40.10. 1 hit.
InterProiIPR008017. Atracotoxin_delta.
IPR004169. Spidertoxin.
[Graphical view]
PfamiPF02819. Toxin_9. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37045-1 [UniParc]FASTAAdd to Basket

« Hide

MKLCMTLLIT AIAVVTFVVA TQEESAEFNE VEESREDNCI AEDYGKCTWG   50
GTKCCRGRPC RCSMIGTNCE CTPRLIMEGL SFA 83
Length:83
Mass (Da):9,167
Last modified:February 1, 1996 - v2
Checksum:i826F5C7E2FDD3CDE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U15925 mRNA. Translation: AAA57116.1.
PIRiA44664.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U15925 mRNA. Translation: AAA57116.1 .
PIRi A44664.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1AGG NMR - A 36-83 [» ]
1OMA NMR - A 36-83 [» ]
1OMB NMR - A 36-83 [» ]
ProteinModelPortali P37045.
SMRi P37045. Positions 36-83.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

ArachnoServeri AS000183. omega-agatoxin-Aa4b.

Miscellaneous databases

EvolutionaryTracei P37045.

Family and domain databases

Gene3Di 4.10.40.10. 1 hit.
InterProi IPR008017. Atracotoxin_delta.
IPR004169. Spidertoxin.
[Graphical view ]
Pfami PF02819. Toxin_9. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Heck S.D., Siok C.J., Krapcho K.J.
    Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Structure and properties of omega-agatoxin IVB, a new antagonist of P-type calcium channels."
    Adams M.E., Mintz I.M., Reily M.D., Thanabal V., Bean B.P.
    Mol. Pharmacol. 44:681-688(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 36-83, FUNCTION, STRUCTURE BY NMR OF 36-83, DISULFIDE BONDS.
    Tissue: Venom.
  3. "A novel peptide from funnel web spider venom, omega-Aga-TK, selectively blocks, P-type calcium channels."
    Teramoto T., Kuwada M., Niidome T., Sawada K., Nishizawa Y., Katayama K.
    Biochem. Biophys. Res. Commun. 196:134-140(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 36-83.
    Tissue: Venom.
  4. "Disulfide bond assignment of omega-agatoxins IVB and IVC: discovery of a D-serine residue in omega-agatoxin IVB."
    Heck S.D., Kelbaugh P.R., Kelly M.E., Thadeio P.F., Saccomano N.A., Stroh J.G., Volkmann R.A.
    J. Am. Chem. Soc. 116:10426-10436(1994)
    Cited for: DISULFIDE BONDS, D-AMINO ACID AT SER-81.
  5. "Functional consequences of posttranslational isomerization of Ser46 in a calcium channel toxin."
    Heck S.D., Siok C.J., Krapcho K.J., Kelbaugh P.R., Thadeio P.F., Welch M.J., Williams R.D., Ganong A.H., Kelly M.E., Lanzetti A.J.
    Science 266:1065-1068(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: D-AMINO ACID AT SER-81, CHARACTERIZATION OF D- AND L- FORMS.
  6. "Omega-agatoxin-TK containing D-serine at position 46, but not synthetic omega-[L-Ser46]agatoxin-TK, exerts blockade of P-type calcium channels in cerebellar Purkinje neurons."
    Kuwada M., Teramoto T., Kumagaye K.Y., Nakajima K., Watanabe T., Kawai T., Kawakami Y., Niidome T., Sawada K., Nishizawa Y.
    Mol. Pharmacol. 46:587-593(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: D-AMINO ACID AT SER-81, CHARACTERIZATION OF D- AND L- FORMS.
  7. "Sequential assignment and structure determination of spider toxin omega-Aga-IVB."
    Yu H., Rosen M.K., Saccomano N.A., Phillips D., Volkmann R.A., Schreiber S.L.
    Biochemistry 32:13123-13129(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 36-83, DISULFIDE BONDS.
  8. "The solution structure of omega-Aga-IVB, a P-type calcium channel antagonist from venom of the funnel web spider, Agelenopsis aperta."
    Reily M.D., Thanabal V., Adams M.E.
    J. Biomol. NMR 5:122-132(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 36-83, DISULFIDE BONDS.

Entry informationi

Entry nameiTOG4B_AGEAP
AccessioniPrimary (citable) accession number: P37045
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3