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P37045

- TOG4B_AGEAP

UniProt

P37045 - TOG4B_AGEAP

Protein

Omega-agatoxin-Aa4b

Gene
N/A
Organism
Agelenopsis aperta (North American funnel-web spider) (Agelenopsis gertschi)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Antagonist of voltage-gated Cav2.1/CACNA1A (P-type) calcium channels. Paralyzes insect by blocking neuromuscular transmission.1 Publication

    GO - Molecular functioni

    1. sodium channel inhibitor activity Source: InterPro

    GO - Biological processi

    1. pathogenesis Source: InterPro

    Keywords - Molecular functioni

    Calcium channel impairing toxin, Ion channel impairing toxin, Neurotoxin, Presynaptic neurotoxin, Toxin, Voltage-gated calcium channel impairing toxin

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Omega-agatoxin-Aa4b
    Short name:
    Omega-AGTX-Aa4b
    Alternative name(s):
    Omega-agatoxin IVB
    Short name:
    Omega-Aga-IVB
    Omega-agatoxin IVC
    Short name:
    Omega-Aga-IVC
    Omega-agatoxin tsukuba
    Short name:
    Omega-Aga-TK
    Short name:
    Omega-agatoxin-TK
    Omega-agatoxin-4B
    OrganismiAgelenopsis aperta (North American funnel-web spider) (Agelenopsis gertschi)
    Taxonomic identifieri6908 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeAraneomorphaeEntelegynaeAgelenidaeAgelenopsis

    Organism-specific databases

    ArachnoServeriAS000183. omega-agatoxin-Aa4b.

    Subcellular locationi

    GO - Cellular componenti

    1. other organism presynaptic membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Propeptidei21 – 3515By similarityPRO_0000035490Add
    BLAST
    Chaini36 – 8348Omega-agatoxin-Aa4bPRO_0000035491Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi39 ↔ 55
    Disulfide bondi47 ↔ 60
    Disulfide bondi54 ↔ 71
    Disulfide bondi62 ↔ 69
    Modified residuei81 – 811D-serine (Ser)3 Publications

    Post-translational modificationi

    The toxin with D-Ser (named omega-aga IVC) is 80-90 fold more potent than that with L-Ser (omega-aga IVB) against Cav2.1/CACNA1A (P-type) channels in rat cerebellar Purkinje neurons and is more resistant to proteases. The epimerization is done by the venom peptide isomerase heterodimer.

    Keywords - PTMi

    D-amino acid, Disulfide bond

    Expressioni

    Tissue specificityi

    Expressed by the venom gland.

    Structurei

    Secondary structure

    1
    83
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi49 – 513
    Turni55 – 573
    Beta strandi60 – 623
    Beta strandi64 – 718
    Turni76 – 794

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1AGGNMR-A36-83[»]
    1OMANMR-A36-83[»]
    1OMBNMR-A36-83[»]
    ProteinModelPortaliP37045.
    SMRiP37045. Positions 36-83.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP37045.

    Family & Domainsi

    Domaini

    The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

    Sequence similaritiesi

    Keywords - Domaini

    Knottin, Signal

    Family and domain databases

    Gene3Di4.10.40.10. 1 hit.
    InterProiIPR008017. Atracotoxin_delta.
    IPR004169. Spidertoxin.
    [Graphical view]
    PfamiPF02819. Toxin_9. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P37045-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLCMTLLIT AIAVVTFVVA TQEESAEFNE VEESREDNCI AEDYGKCTWG   50
    GTKCCRGRPC RCSMIGTNCE CTPRLIMEGL SFA 83
    Length:83
    Mass (Da):9,167
    Last modified:February 1, 1996 - v2
    Checksum:i826F5C7E2FDD3CDE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U15925 mRNA. Translation: AAA57116.1.
    PIRiA44664.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U15925 mRNA. Translation: AAA57116.1 .
    PIRi A44664.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1AGG NMR - A 36-83 [» ]
    1OMA NMR - A 36-83 [» ]
    1OMB NMR - A 36-83 [» ]
    ProteinModelPortali P37045.
    SMRi P37045. Positions 36-83.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    ArachnoServeri AS000183. omega-agatoxin-Aa4b.

    Miscellaneous databases

    EvolutionaryTracei P37045.

    Family and domain databases

    Gene3Di 4.10.40.10. 1 hit.
    InterProi IPR008017. Atracotoxin_delta.
    IPR004169. Spidertoxin.
    [Graphical view ]
    Pfami PF02819. Toxin_9. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Heck S.D., Siok C.J., Krapcho K.J.
      Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Structure and properties of omega-agatoxin IVB, a new antagonist of P-type calcium channels."
      Adams M.E., Mintz I.M., Reily M.D., Thanabal V., Bean B.P.
      Mol. Pharmacol. 44:681-688(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 36-83, FUNCTION, STRUCTURE BY NMR OF 36-83, DISULFIDE BONDS.
      Tissue: Venom.
    3. "A novel peptide from funnel web spider venom, omega-Aga-TK, selectively blocks, P-type calcium channels."
      Teramoto T., Kuwada M., Niidome T., Sawada K., Nishizawa Y., Katayama K.
      Biochem. Biophys. Res. Commun. 196:134-140(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 36-83.
      Tissue: Venom.
    4. "Disulfide bond assignment of omega-agatoxins IVB and IVC: discovery of a D-serine residue in omega-agatoxin IVB."
      Heck S.D., Kelbaugh P.R., Kelly M.E., Thadeio P.F., Saccomano N.A., Stroh J.G., Volkmann R.A.
      J. Am. Chem. Soc. 116:10426-10436(1994)
      Cited for: DISULFIDE BONDS, D-AMINO ACID AT SER-81.
    5. "Functional consequences of posttranslational isomerization of Ser46 in a calcium channel toxin."
      Heck S.D., Siok C.J., Krapcho K.J., Kelbaugh P.R., Thadeio P.F., Welch M.J., Williams R.D., Ganong A.H., Kelly M.E., Lanzetti A.J.
      Science 266:1065-1068(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: D-AMINO ACID AT SER-81, CHARACTERIZATION OF D- AND L- FORMS.
    6. "Omega-agatoxin-TK containing D-serine at position 46, but not synthetic omega-[L-Ser46]agatoxin-TK, exerts blockade of P-type calcium channels in cerebellar Purkinje neurons."
      Kuwada M., Teramoto T., Kumagaye K.Y., Nakajima K., Watanabe T., Kawai T., Kawakami Y., Niidome T., Sawada K., Nishizawa Y.
      Mol. Pharmacol. 46:587-593(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: D-AMINO ACID AT SER-81, CHARACTERIZATION OF D- AND L- FORMS.
    7. "Sequential assignment and structure determination of spider toxin omega-Aga-IVB."
      Yu H., Rosen M.K., Saccomano N.A., Phillips D., Volkmann R.A., Schreiber S.L.
      Biochemistry 32:13123-13129(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 36-83, DISULFIDE BONDS.
    8. "The solution structure of omega-Aga-IVB, a P-type calcium channel antagonist from venom of the funnel web spider, Agelenopsis aperta."
      Reily M.D., Thanabal V., Adams M.E.
      J. Biomol. NMR 5:122-132(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 36-83, DISULFIDE BONDS.

    Entry informationi

    Entry nameiTOG4B_AGEAP
    AccessioniPrimary (citable) accession number: P37045
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 82 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programAnimal Toxin Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3