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P37045 (TOG4B_AGEAP) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Omega-agatoxin-Aa4b

Short name=Omega-AGTX-Aa4b
Alternative name(s):
Omega-agatoxin IVB
Short name=Omega-Aga-IVB
Omega-agatoxin IVC
Short name=Omega-Aga-IVC
Omega-agatoxin tsukuba
Short name=Omega-Aga-TK
Short name=Omega-agatoxin-TK
Omega-agatoxin-4B
OrganismAgelenopsis aperta (North American funnel-web spider) (Agelenopsis gertschi)
Taxonomic identifier6908 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeAraneomorphaeEntelegynaeAgelenidaeAgelenopsis

Protein attributes

Sequence length83 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Antagonist of voltage-gated Cav2.1/CACNA1A (P-type) calcium channels. Paralyzes insect by blocking neuromuscular transmission. Ref.2

Subcellular location

Secreted.

Tissue specificity

Expressed by the venom gland.

Domain

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Post-translational modification

The toxin with D-Ser (named omega-aga IVC) is 80-90 fold more potent than that with L-Ser (omega-aga IVB) against Cav2.1/CACNA1A (P-type) channels in rat cerebellar Purkinje neurons and is more resistant to proteases. The epimerization is done by the venom peptide isomerase heterodimer.

Sequence similarities

Belongs to the plectoxin superfamily. Type IV omega-agatoxin family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Propeptide21 – 3515 By similarity
PRO_0000035490
Chain36 – 8348Omega-agatoxin-Aa4b
PRO_0000035491

Amino acid modifications

Modified residue811D-serine (Ser); by venom peptide isomerase Ref.4 Ref.5 Ref.6
Disulfide bond39 ↔ 55 Ref.2 Ref.4 Ref.7 Ref.8
Disulfide bond47 ↔ 60 Ref.2 Ref.4 Ref.7 Ref.8
Disulfide bond54 ↔ 71 Ref.2 Ref.4 Ref.7 Ref.8
Disulfide bond62 ↔ 69 Ref.2 Ref.4 Ref.7 Ref.8

Secondary structure

........... 83
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P37045 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: 826F5C7E2FDD3CDE

FASTA839,167
        10         20         30         40         50         60 
MKLCMTLLIT AIAVVTFVVA TQEESAEFNE VEESREDNCI AEDYGKCTWG GTKCCRGRPC 

        70         80 
RCSMIGTNCE CTPRLIMEGL SFA 

« Hide

References

[1]Heck S.D., Siok C.J., Krapcho K.J.
Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Structure and properties of omega-agatoxin IVB, a new antagonist of P-type calcium channels."
Adams M.E., Mintz I.M., Reily M.D., Thanabal V., Bean B.P.
Mol. Pharmacol. 44:681-688(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 36-83, FUNCTION, STRUCTURE BY NMR OF 36-83, DISULFIDE BONDS.
Tissue: Venom.
[3]"A novel peptide from funnel web spider venom, omega-Aga-TK, selectively blocks, P-type calcium channels."
Teramoto T., Kuwada M., Niidome T., Sawada K., Nishizawa Y., Katayama K.
Biochem. Biophys. Res. Commun. 196:134-140(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 36-83.
Tissue: Venom.
[4]"Disulfide bond assignment of omega-agatoxins IVB and IVC: discovery of a D-serine residue in omega-agatoxin IVB."
Heck S.D., Kelbaugh P.R., Kelly M.E., Thadeio P.F., Saccomano N.A., Stroh J.G., Volkmann R.A.
J. Am. Chem. Soc. 116:10426-10436(1994)
Cited for: DISULFIDE BONDS, D-AMINO ACID AT SER-81.
[5]"Functional consequences of posttranslational isomerization of Ser46 in a calcium channel toxin."
Heck S.D., Siok C.J., Krapcho K.J., Kelbaugh P.R., Thadeio P.F., Welch M.J., Williams R.D., Ganong A.H., Kelly M.E., Lanzetti A.J.
Science 266:1065-1068(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: D-AMINO ACID AT SER-81, CHARACTERIZATION OF D- AND L- FORMS.
[6]"Omega-agatoxin-TK containing D-serine at position 46, but not synthetic omega-[L-Ser46]agatoxin-TK, exerts blockade of P-type calcium channels in cerebellar Purkinje neurons."
Kuwada M., Teramoto T., Kumagaye K.Y., Nakajima K., Watanabe T., Kawai T., Kawakami Y., Niidome T., Sawada K., Nishizawa Y.
Mol. Pharmacol. 46:587-593(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: D-AMINO ACID AT SER-81, CHARACTERIZATION OF D- AND L- FORMS.
[7]"Sequential assignment and structure determination of spider toxin omega-Aga-IVB."
Yu H., Rosen M.K., Saccomano N.A., Phillips D., Volkmann R.A., Schreiber S.L.
Biochemistry 32:13123-13129(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 36-83, DISULFIDE BONDS.
[8]"The solution structure of omega-Aga-IVB, a P-type calcium channel antagonist from venom of the funnel web spider, Agelenopsis aperta."
Reily M.D., Thanabal V., Adams M.E.
J. Biomol. NMR 5:122-132(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 36-83, DISULFIDE BONDS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U15925 mRNA. Translation: AAA57116.1.
PIRA44664.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1AGGNMR-A36-83[»]
1OMANMR-A36-83[»]
1OMBNMR-A36-83[»]
ProteinModelPortalP37045.
SMRP37045. Positions 36-83.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

ArachnoServerAS000183. omega-agatoxin-Aa4b.

Family and domain databases

Gene3D4.10.40.10. 1 hit.
InterProIPR008017. Atracotoxin_delta.
IPR004169. Spidertoxin.
[Graphical view]
PfamPF02819. Toxin_9. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP37045.

Entry information

Entry nameTOG4B_AGEAP
AccessionPrimary (citable) accession number: P37045
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: February 1, 1996
Last modified: October 16, 2013
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references