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Protein

Omega-agatoxin-Aa4b

Gene
N/A
Organism
Agelenopsis aperta (North American funnel-web spider) (Agelenopsis gertschi)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Antagonist of voltage-gated Cav2.1/CACNA1A (P-type) calcium channels. Paralyzes insect by blocking neuromuscular transmission.1 Publication

GO - Molecular functioni

Keywordsi

Molecular functionCalcium channel impairing toxin, Ion channel impairing toxin, Neurotoxin, Presynaptic neurotoxin, Toxin, Voltage-gated calcium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Omega-agatoxin-Aa4b
Short name:
Omega-AGTX-Aa4b
Alternative name(s):
Omega-agatoxin IVB
Short name:
Omega-Aga-IVB
Omega-agatoxin IVC
Short name:
Omega-Aga-IVC
Omega-agatoxin tsukuba
Short name:
Omega-Aga-TK
Short name:
Omega-agatoxin-TK
Omega-agatoxin-4B
OrganismiAgelenopsis aperta (North American funnel-web spider) (Agelenopsis gertschi)
Taxonomic identifieri6908 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeAraneomorphaeEntelegynaeAgelenidaeAgelenopsis

Organism-specific databases

ArachnoServeriAS000183 omega-agatoxin-Aa4b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
PropeptideiPRO_000003549021 – 35By similarityAdd BLAST15
ChainiPRO_000003549136 – 83Omega-agatoxin-Aa4bAdd BLAST48

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi39 ↔ 55
Disulfide bondi47 ↔ 60
Disulfide bondi54 ↔ 71
Disulfide bondi62 ↔ 69
Modified residuei81D-serine (Ser)3 Publications1

Post-translational modificationi

The toxin with D-Ser (named omega-aga IVC) is 80-90 fold more potent than that with L-Ser (omega-aga IVB) against Cav2.1/CACNA1A (P-type) channels in rat cerebellar Purkinje neurons and is more resistant to proteases. The epimerization is done by the venom peptide isomerase heterodimer.

Keywords - PTMi

D-amino acid, Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

183
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi49 – 51Combined sources3
Turni55 – 57Combined sources3
Beta strandi60 – 62Combined sources3
Beta strandi64 – 71Combined sources8
Turni76 – 79Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AGGNMR-A36-83[»]
1OMANMR-A36-83[»]
1OMBNMR-A36-83[»]
ProteinModelPortaliP37045
SMRiP37045
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37045

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Sequence similaritiesi

Keywords - Domaini

Knottin, Signal

Family and domain databases

CDDicd12960 Spider_toxin, 1 hit
InterProiView protein in InterPro
IPR004169 Spidertoxin

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37045-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLCMTLLIT AIAVVTFVVA TQEESAEFNE VEESREDNCI AEDYGKCTWG
60 70 80
GTKCCRGRPC RCSMIGTNCE CTPRLIMEGL SFA
Length:83
Mass (Da):9,167
Last modified:February 1, 1996 - v2
Checksum:i826F5C7E2FDD3CDE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15925 mRNA Translation: AAA57116.1
PIRiA44664

Entry informationi

Entry nameiTX23B_AGEAP
AccessioniPrimary (citable) accession number: P37045
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: February 1, 1996
Last modified: June 20, 2018
This is version 96 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

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