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P37040

- NCPR_MOUSE

UniProt

P37040 - NCPR_MOUSE

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Protein
NADPH--cytochrome P450 reductase
Gene
Por
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.

Cofactori

FAD.
FMN.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi170 – 20132FMN By similarity
Add
BLAST
Nucleotide bindingi314 – 32512FAD By similarity
Add
BLAST
Nucleotide bindingi451 – 46111FAD By similarity
Add
BLAST
Nucleotide bindingi530 – 54819NADP By similarity
Add
BLAST
Nucleotide bindingi625 – 64117NADP By similarity
Add
BLAST

GO - Molecular functioni

  1. FMN binding Source: Ensembl
  2. NADP binding Source: Ensembl
  3. NADPH-hemoprotein reductase activity Source: RefGenome
  4. cytochrome-b5 reductase activity, acting on NAD(P)H Source: Ensembl
  5. electron carrier activity Source: Ensembl
  6. flavin adenine dinucleotide binding Source: Ensembl
  7. hydrolase activity Source: Ensembl
  8. iron ion binding Source: InterPro
  9. iron-cytochrome-c reductase activity Source: Ensembl
  10. nitric oxide dioxygenase activity Source: Ensembl
  11. oxidoreductase activity Source: MGI
  12. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen Source: RefGenome

GO - Biological processi

  1. carnitine metabolic process Source: Ensembl
  2. cellular organofluorine metabolic process Source: Ensembl
  3. cellular response to follicle-stimulating hormone stimulus Source: Ensembl
  4. cellular response to peptide hormone stimulus Source: Ensembl
  5. demethylation Source: Ensembl
  6. fatty acid oxidation Source: Ensembl
  7. flavonoid metabolic process Source: Ensembl
  8. internal peptidyl-lysine acetylation Source: Ensembl
  9. negative regulation of apoptotic process Source: Ensembl
  10. negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
  11. negative regulation of lipase activity Source: Ensembl
  12. nitrate catabolic process Source: Ensembl
  13. nitric oxide catabolic process Source: Ensembl
  14. positive regulation of cholesterol biosynthetic process Source: Ensembl
  15. positive regulation of chondrocyte differentiation Source: Ensembl
  16. positive regulation of monooxygenase activity Source: Ensembl
  17. positive regulation of smoothened signaling pathway Source: Ensembl
  18. positive regulation of steroid hormone biosynthetic process Source: Ensembl
  19. regulation of growth plate cartilage chondrocyte proliferation Source: Ensembl
  20. response to drug Source: Ensembl
  21. response to nutrient Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductase (EC:1.6.2.4)
Short name:
CPR
Short name:
P450R
Gene namesi
Name:Por
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:97744. Por.

Subcellular locationi

Endoplasmic reticulum membrane; Peripheral membrane protein
Note: Anchored to the ER membrane by its N-terminal hydrophobic region.

GO - Cellular componenti

  1. cytosol Source: RefGenome
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. mitochondrion Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 678677NADPH--cytochrome P450 reductase
PRO_0000167597Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylglycine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP37040.
PaxDbiP37040.
PRIDEiP37040.

2D gel databases

SWISS-2DPAGEP37040.

PTM databases

PhosphoSiteiP37040.

Expressioni

Gene expression databases

ArrayExpressiP37040.
BgeeiP37040.
CleanExiMM_POR.
GenevestigatoriP37040.

Interactioni

Protein-protein interaction databases

BioGridi202299. 1 interaction.
IntActiP37040. 6 interactions.
MINTiMINT-1863828.
STRINGi10090.ENSMUSP00000005651.

Structurei

3D structure databases

ProteinModelPortaliP37040.
SMRiP37040. Positions 63-678.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini80 – 224145Flavodoxin-like
Add
BLAST
Domaini279 – 521243FAD-binding FR-type
Add
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

Phylogenomic databases

eggNOGiCOG0369.
GeneTreeiENSGT00620000087711.
HOGENOMiHOG000282027.
HOVERGENiHBG000432.
InParanoidiP37040.
KOiK00327.
OMAiEFGAMEH.
OrthoDBiEOG7HQN7J.
PhylomeDBiP37040.
TreeFamiTF105719.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37040-1 [UniParc]FASTAAdd to Basket

« Hide

MGDSHEDTSA TVPEAVAEEV SLFSTTDIVL FSLIVGVLTY WFIFKKKKEE    50
IPEFSKIQTT APPVKESSFV EKMKKTGRNI IVFYGSQTGT AEEFANRLSK 100
DAHRYGMRGM SADPEEYDLA DLSSLPEIDK SLVVFCMATY GEGDPTDNAQ 150
DFYDWLQETD VDLTGVKFAV FGLGNKTYEH FNAMGKYVDQ RLEQLGAQRI 200
FELGLGDDDG NLEEDFITWR EQFWPAVCEF FGVEATGEES SIRQYELVVH 250
EDMDTAKVYT GEMGRLKSYE NQKPPFDAKN PFLAAVTTNR KLNQGTERHL 300
MHLELDISDS KIRYESGDHV AVYPANDSTL VNQIGEILGA DLDVIMSLNN 350
LDEESNKKHP FPCPTTYRTA LTYYLDITNP PRTNVLYELA QYASEPSEQE 400
HLHKMASSSG EGKELYLSWV VEARRHILAI LQDYPSLRPP IDHLCELLPR 450
LQARYYSIAS SSKVHPNSVH ICAVAVEYEA KSGRVNKGVA TSWLRTKEPA 500
GENGRRALVP MFVRKSQFRL PFKPTTPVIM VGPGTGVAPF MGFIQERAWL 550
REQGKEVGET LLYYGCRRSD EDYLYREELA RFHKDGALTQ LNVAFSREQA 600
HKVYVQHLLK RDKEHLWKLI HEGGAHIYVC GDARNMAKDV QNTFYDIVAE 650
FGPMEHTQAV DYVKKLMTKG RYSLDVWS 678
Length:678
Mass (Da):77,044
Last modified:January 23, 2007 - v2
Checksum:i060282A7A55483AF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D17571 mRNA. Translation: BAA04496.1.
BC031463 mRNA. Translation: AAH31463.1.
CCDSiCCDS39318.1.
RefSeqiNP_032924.1. NM_008898.1.
XP_006504461.1. XM_006504398.1.
XP_006504462.1. XM_006504399.1.
UniGeneiMm.3863.

Genome annotation databases

EnsembliENSMUST00000005651; ENSMUSP00000005651; ENSMUSG00000005514.
GeneIDi18984.
KEGGimmu:18984.
UCSCiuc008zyt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D17571 mRNA. Translation: BAA04496.1 .
BC031463 mRNA. Translation: AAH31463.1 .
CCDSi CCDS39318.1.
RefSeqi NP_032924.1. NM_008898.1.
XP_006504461.1. XM_006504398.1.
XP_006504462.1. XM_006504399.1.
UniGenei Mm.3863.

3D structure databases

ProteinModelPortali P37040.
SMRi P37040. Positions 63-678.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 202299. 1 interaction.
IntActi P37040. 6 interactions.
MINTi MINT-1863828.
STRINGi 10090.ENSMUSP00000005651.

PTM databases

PhosphoSitei P37040.

2D gel databases

SWISS-2DPAGE P37040.

Proteomic databases

MaxQBi P37040.
PaxDbi P37040.
PRIDEi P37040.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000005651 ; ENSMUSP00000005651 ; ENSMUSG00000005514 .
GeneIDi 18984.
KEGGi mmu:18984.
UCSCi uc008zyt.1. mouse.

Organism-specific databases

CTDi 5447.
MGIi MGI:97744. Por.

Phylogenomic databases

eggNOGi COG0369.
GeneTreei ENSGT00620000087711.
HOGENOMi HOG000282027.
HOVERGENi HBG000432.
InParanoidi P37040.
KOi K00327.
OMAi EFGAMEH.
OrthoDBi EOG7HQN7J.
PhylomeDBi P37040.
TreeFami TF105719.

Miscellaneous databases

ChiTaRSi POR. mouse.
NextBioi 295354.
PROi P37040.
SOURCEi Search...

Gene expression databases

ArrayExpressi P37040.
Bgeei P37040.
CleanExi MM_POR.
Genevestigatori P37040.

Family and domain databases

Gene3Di 1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProi IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF000208. P450R. 1 hit.
PRINTSi PR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMi SSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEi PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse NADPH-cytochrome P-450 oxidoreductase: molecular cloning and functional expression in yeast."
    Ohgiya S., Ishizaki K., Kamataki T., Shinriki N.
    Biochim. Biophys. Acta 1186:137-141(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ddY.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.

Entry informationi

Entry nameiNCPR_MOUSE
AccessioniPrimary (citable) accession number: P37040
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi