Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P37040

- NCPR_MOUSE

UniProt

P37040 - NCPR_MOUSE

Protein

NADPH--cytochrome P450 reductase

Gene

Por

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.

    Catalytic activityi

    NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.

    Cofactori

    FAD.
    FMN.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi170 – 20132FMNPROSITE-ProRule annotationAdd
    BLAST
    Nucleotide bindingi314 – 32512FADBy similarityAdd
    BLAST
    Nucleotide bindingi451 – 46111FADBy similarityAdd
    BLAST
    Nucleotide bindingi530 – 54819NADPBy similarityAdd
    BLAST
    Nucleotide bindingi625 – 64117NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. cytochrome-b5 reductase activity, acting on NAD(P)H Source: Ensembl
    2. electron carrier activity Source: Ensembl
    3. flavin adenine dinucleotide binding Source: Ensembl
    4. FMN binding Source: Ensembl
    5. hydrolase activity Source: Ensembl
    6. iron-cytochrome-c reductase activity Source: Ensembl
    7. iron ion binding Source: InterPro
    8. NADP binding Source: Ensembl
    9. NADPH-hemoprotein reductase activity Source: RefGenome
    10. nitric oxide dioxygenase activity Source: Ensembl
    11. oxidoreductase activity Source: MGI
    12. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen Source: RefGenome

    GO - Biological processi

    1. carnitine metabolic process Source: Ensembl
    2. cellular organofluorine metabolic process Source: Ensembl
    3. cellular response to follicle-stimulating hormone stimulus Source: Ensembl
    4. cellular response to peptide hormone stimulus Source: Ensembl
    5. demethylation Source: Ensembl
    6. fatty acid oxidation Source: Ensembl
    7. flavonoid metabolic process Source: Ensembl
    8. internal peptidyl-lysine acetylation Source: Ensembl
    9. negative regulation of apoptotic process Source: Ensembl
    10. negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
    11. negative regulation of lipase activity Source: Ensembl
    12. nitrate catabolic process Source: Ensembl
    13. nitric oxide catabolic process Source: Ensembl
    14. positive regulation of cholesterol biosynthetic process Source: Ensembl
    15. positive regulation of chondrocyte differentiation Source: Ensembl
    16. positive regulation of monooxygenase activity Source: Ensembl
    17. positive regulation of smoothened signaling pathway Source: Ensembl
    18. positive regulation of steroid hormone biosynthetic process Source: Ensembl
    19. regulation of growth plate cartilage chondrocyte proliferation Source: Ensembl
    20. response to drug Source: Ensembl
    21. response to nutrient Source: Ensembl

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein, FMN, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADPH--cytochrome P450 reductase (EC:1.6.2.4)
    Short name:
    CPR
    Short name:
    P450R
    Gene namesi
    Name:Por
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:97744. Por.

    Subcellular locationi

    Endoplasmic reticulum membrane; Peripheral membrane protein
    Note: Anchored to the ER membrane by its N-terminal hydrophobic region.

    GO - Cellular componenti

    1. cytosol Source: RefGenome
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. mitochondrion Source: Ensembl

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 678677NADPH--cytochrome P450 reductasePRO_0000167597Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylglycineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP37040.
    PaxDbiP37040.
    PRIDEiP37040.

    2D gel databases

    SWISS-2DPAGEP37040.

    PTM databases

    PhosphoSiteiP37040.

    Expressioni

    Gene expression databases

    ArrayExpressiP37040.
    BgeeiP37040.
    CleanExiMM_POR.
    GenevestigatoriP37040.

    Interactioni

    Protein-protein interaction databases

    BioGridi202299. 1 interaction.
    IntActiP37040. 6 interactions.
    MINTiMINT-1863828.
    STRINGi10090.ENSMUSP00000005651.

    Structurei

    3D structure databases

    ProteinModelPortaliP37040.
    SMRiP37040. Positions 63-678.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini80 – 224145Flavodoxin-likePROSITE-ProRule annotationAdd
    BLAST
    Domaini279 – 521243FAD-binding FR-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.Curated
    Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
    Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0369.
    GeneTreeiENSGT00620000087711.
    HOGENOMiHOG000282027.
    HOVERGENiHBG000432.
    InParanoidiP37040.
    KOiK00327.
    OMAiEFGAMEH.
    OrthoDBiEOG7HQN7J.
    PhylomeDBiP37040.
    TreeFamiTF105719.

    Family and domain databases

    Gene3Di1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    InterProiIPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR023208. P450R.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view]
    PfamiPF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000208. P450R. 1 hit.
    PRINTSiPR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMiSSF52218. SSF52218. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEiPS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P37040-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGDSHEDTSA TVPEAVAEEV SLFSTTDIVL FSLIVGVLTY WFIFKKKKEE    50
    IPEFSKIQTT APPVKESSFV EKMKKTGRNI IVFYGSQTGT AEEFANRLSK 100
    DAHRYGMRGM SADPEEYDLA DLSSLPEIDK SLVVFCMATY GEGDPTDNAQ 150
    DFYDWLQETD VDLTGVKFAV FGLGNKTYEH FNAMGKYVDQ RLEQLGAQRI 200
    FELGLGDDDG NLEEDFITWR EQFWPAVCEF FGVEATGEES SIRQYELVVH 250
    EDMDTAKVYT GEMGRLKSYE NQKPPFDAKN PFLAAVTTNR KLNQGTERHL 300
    MHLELDISDS KIRYESGDHV AVYPANDSTL VNQIGEILGA DLDVIMSLNN 350
    LDEESNKKHP FPCPTTYRTA LTYYLDITNP PRTNVLYELA QYASEPSEQE 400
    HLHKMASSSG EGKELYLSWV VEARRHILAI LQDYPSLRPP IDHLCELLPR 450
    LQARYYSIAS SSKVHPNSVH ICAVAVEYEA KSGRVNKGVA TSWLRTKEPA 500
    GENGRRALVP MFVRKSQFRL PFKPTTPVIM VGPGTGVAPF MGFIQERAWL 550
    REQGKEVGET LLYYGCRRSD EDYLYREELA RFHKDGALTQ LNVAFSREQA 600
    HKVYVQHLLK RDKEHLWKLI HEGGAHIYVC GDARNMAKDV QNTFYDIVAE 650
    FGPMEHTQAV DYVKKLMTKG RYSLDVWS 678
    Length:678
    Mass (Da):77,044
    Last modified:January 23, 2007 - v2
    Checksum:i060282A7A55483AF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D17571 mRNA. Translation: BAA04496.1.
    BC031463 mRNA. Translation: AAH31463.1.
    CCDSiCCDS39318.1.
    RefSeqiNP_032924.1. NM_008898.1.
    XP_006504461.1. XM_006504398.1.
    XP_006504462.1. XM_006504399.1.
    UniGeneiMm.3863.

    Genome annotation databases

    EnsembliENSMUST00000005651; ENSMUSP00000005651; ENSMUSG00000005514.
    GeneIDi18984.
    KEGGimmu:18984.
    UCSCiuc008zyt.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D17571 mRNA. Translation: BAA04496.1 .
    BC031463 mRNA. Translation: AAH31463.1 .
    CCDSi CCDS39318.1.
    RefSeqi NP_032924.1. NM_008898.1.
    XP_006504461.1. XM_006504398.1.
    XP_006504462.1. XM_006504399.1.
    UniGenei Mm.3863.

    3D structure databases

    ProteinModelPortali P37040.
    SMRi P37040. Positions 63-678.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202299. 1 interaction.
    IntActi P37040. 6 interactions.
    MINTi MINT-1863828.
    STRINGi 10090.ENSMUSP00000005651.

    PTM databases

    PhosphoSitei P37040.

    2D gel databases

    SWISS-2DPAGE P37040.

    Proteomic databases

    MaxQBi P37040.
    PaxDbi P37040.
    PRIDEi P37040.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000005651 ; ENSMUSP00000005651 ; ENSMUSG00000005514 .
    GeneIDi 18984.
    KEGGi mmu:18984.
    UCSCi uc008zyt.1. mouse.

    Organism-specific databases

    CTDi 5447.
    MGIi MGI:97744. Por.

    Phylogenomic databases

    eggNOGi COG0369.
    GeneTreei ENSGT00620000087711.
    HOGENOMi HOG000282027.
    HOVERGENi HBG000432.
    InParanoidi P37040.
    KOi K00327.
    OMAi EFGAMEH.
    OrthoDBi EOG7HQN7J.
    PhylomeDBi P37040.
    TreeFami TF105719.

    Miscellaneous databases

    ChiTaRSi POR. mouse.
    NextBioi 295354.
    PROi P37040.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P37040.
    Bgeei P37040.
    CleanExi MM_POR.
    Genevestigatori P37040.

    Family and domain databases

    Gene3Di 1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    InterProi IPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR023208. P450R.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view ]
    Pfami PF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000208. P450R. 1 hit.
    PRINTSi PR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMi SSF52218. SSF52218. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEi PS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse NADPH-cytochrome P-450 oxidoreductase: molecular cloning and functional expression in yeast."
      Ohgiya S., Ishizaki K., Kamataki T., Shinriki N.
      Biochim. Biophys. Acta 1186:137-141(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: ddY.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary gland.

    Entry informationi

    Entry nameiNCPR_MOUSE
    AccessioniPrimary (citable) accession number: P37040
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 127 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3