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Protein

NADPH--cytochrome P450 reductase

Gene

Por

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.UniRule annotation

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • FADUniRule annotationNote: Binds 1 FAD per monomer.UniRule annotation
  • FMNUniRule annotationNote: Binds 1 FMN per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei208 – 2081FMNUniRule annotation
Binding sitei298 – 2981NADPUniRule annotation
Binding sitei424 – 4241FADUniRule annotation
Binding sitei478 – 4781FADUniRule annotation
Binding sitei535 – 5351NADPUniRule annotation
Binding sitei639 – 6391NADPUniRule annotation
Binding sitei677 – 6771FADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi86 – 916FMNUniRule annotation
Nucleotide bindingi138 – 1414FMNUniRule annotation
Nucleotide bindingi173 – 18210FMNUniRule annotation
Nucleotide bindingi454 – 4574FADUniRule annotation
Nucleotide bindingi472 – 4743FADUniRule annotation
Nucleotide bindingi488 – 4914FADUniRule annotation
Nucleotide bindingi596 – 5972NADPUniRule annotation
Nucleotide bindingi602 – 6065NADPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductaseUniRule annotation (EC:1.6.2.4UniRule annotation)
Short name:
CPRUniRule annotation
Short name:
P450RUniRule annotation
Gene namesi
Name:PorUniRule annotation
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:97744. Por.

Subcellular locationi

  • Endoplasmic reticulum membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 2221LumenalUniRule annotationAdd
BLAST
Transmembranei23 – 4321HelicalUniRule annotationAdd
BLAST
Topological domaini44 – 678635CytoplasmicUniRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 678677NADPH--cytochrome P450 reductasePRO_0000167597Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylglycineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP37040.
PaxDbiP37040.
PRIDEiP37040.

2D gel databases

SWISS-2DPAGEP37040.

PTM databases

iPTMnetiP37040.
PhosphoSiteiP37040.

Expressioni

Gene expression databases

BgeeiP37040.
CleanExiMM_POR.
ExpressionAtlasiP37040. baseline.
GenevisibleiP37040. MM.

Interactioni

Protein-protein interaction databases

BioGridi202299. 1 interaction.
IntActiP37040. 6 interactions.
MINTiMINT-1863828.
STRINGi10090.ENSMUSP00000005651.

Structurei

3D structure databases

ProteinModelPortaliP37040.
SMRiP37040. Positions 63-678.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini80 – 224145Flavodoxin-likeUniRule annotationAdd
BLAST
Domaini279 – 521243FAD-binding FR-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the NADPH--cytochrome P450 reductase family.UniRule annotation
In the N-terminal section; belongs to the flavodoxin family.UniRule annotation
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation
Contains 1 FAD-binding FR-type domain.UniRule annotation
Contains 1 flavodoxin-like domain.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1158. Eukaryota.
COG0369. LUCA.
GeneTreeiENSGT00620000087711.
HOGENOMiHOG000282027.
HOVERGENiHBG000432.
InParanoidiP37040.
KOiK00327.
OMAiSEESQEC.
OrthoDBiEOG7HQN7J.
PhylomeDBiP37040.
TreeFamiTF105719.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03212. NCPR.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37040-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDSHEDTSA TVPEAVAEEV SLFSTTDIVL FSLIVGVLTY WFIFKKKKEE
60 70 80 90 100
IPEFSKIQTT APPVKESSFV EKMKKTGRNI IVFYGSQTGT AEEFANRLSK
110 120 130 140 150
DAHRYGMRGM SADPEEYDLA DLSSLPEIDK SLVVFCMATY GEGDPTDNAQ
160 170 180 190 200
DFYDWLQETD VDLTGVKFAV FGLGNKTYEH FNAMGKYVDQ RLEQLGAQRI
210 220 230 240 250
FELGLGDDDG NLEEDFITWR EQFWPAVCEF FGVEATGEES SIRQYELVVH
260 270 280 290 300
EDMDTAKVYT GEMGRLKSYE NQKPPFDAKN PFLAAVTTNR KLNQGTERHL
310 320 330 340 350
MHLELDISDS KIRYESGDHV AVYPANDSTL VNQIGEILGA DLDVIMSLNN
360 370 380 390 400
LDEESNKKHP FPCPTTYRTA LTYYLDITNP PRTNVLYELA QYASEPSEQE
410 420 430 440 450
HLHKMASSSG EGKELYLSWV VEARRHILAI LQDYPSLRPP IDHLCELLPR
460 470 480 490 500
LQARYYSIAS SSKVHPNSVH ICAVAVEYEA KSGRVNKGVA TSWLRTKEPA
510 520 530 540 550
GENGRRALVP MFVRKSQFRL PFKPTTPVIM VGPGTGVAPF MGFIQERAWL
560 570 580 590 600
REQGKEVGET LLYYGCRRSD EDYLYREELA RFHKDGALTQ LNVAFSREQA
610 620 630 640 650
HKVYVQHLLK RDKEHLWKLI HEGGAHIYVC GDARNMAKDV QNTFYDIVAE
660 670
FGPMEHTQAV DYVKKLMTKG RYSLDVWS
Length:678
Mass (Da):77,044
Last modified:January 23, 2007 - v2
Checksum:i060282A7A55483AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17571 mRNA. Translation: BAA04496.1.
BC031463 mRNA. Translation: AAH31463.1.
CCDSiCCDS39318.1.
RefSeqiNP_032924.1. NM_008898.2.
XP_006504461.1. XM_006504398.1.
XP_006504462.1. XM_006504399.1.
UniGeneiMm.3863.

Genome annotation databases

EnsembliENSMUST00000005651; ENSMUSP00000005651; ENSMUSG00000005514.
GeneIDi18984.
KEGGimmu:18984.
UCSCiuc008zyt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17571 mRNA. Translation: BAA04496.1.
BC031463 mRNA. Translation: AAH31463.1.
CCDSiCCDS39318.1.
RefSeqiNP_032924.1. NM_008898.2.
XP_006504461.1. XM_006504398.1.
XP_006504462.1. XM_006504399.1.
UniGeneiMm.3863.

3D structure databases

ProteinModelPortaliP37040.
SMRiP37040. Positions 63-678.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202299. 1 interaction.
IntActiP37040. 6 interactions.
MINTiMINT-1863828.
STRINGi10090.ENSMUSP00000005651.

PTM databases

iPTMnetiP37040.
PhosphoSiteiP37040.

2D gel databases

SWISS-2DPAGEP37040.

Proteomic databases

MaxQBiP37040.
PaxDbiP37040.
PRIDEiP37040.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005651; ENSMUSP00000005651; ENSMUSG00000005514.
GeneIDi18984.
KEGGimmu:18984.
UCSCiuc008zyt.1. mouse.

Organism-specific databases

CTDi5447.
MGIiMGI:97744. Por.

Phylogenomic databases

eggNOGiKOG1158. Eukaryota.
COG0369. LUCA.
GeneTreeiENSGT00620000087711.
HOGENOMiHOG000282027.
HOVERGENiHBG000432.
InParanoidiP37040.
KOiK00327.
OMAiSEESQEC.
OrthoDBiEOG7HQN7J.
PhylomeDBiP37040.
TreeFamiTF105719.

Miscellaneous databases

ChiTaRSiPor. mouse.
NextBioi295354.
PROiP37040.
SOURCEiSearch...

Gene expression databases

BgeeiP37040.
CleanExiMM_POR.
ExpressionAtlasiP37040. baseline.
GenevisibleiP37040. MM.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03212. NCPR.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse NADPH-cytochrome P-450 oxidoreductase: molecular cloning and functional expression in yeast."
    Ohgiya S., Ishizaki K., Kamataki T., Shinriki N.
    Biochim. Biophys. Acta 1186:137-141(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ddY.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiNCPR_MOUSE
AccessioniPrimary (citable) accession number: P37040
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: January 20, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.