Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P37040

- NCPR_MOUSE

UniProt

P37040 - NCPR_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

NADPH--cytochrome P450 reductase

Gene

Por

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.

Cofactori

Protein has several cofactor binding sites:

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi170 – 20132FMNPROSITE-ProRule annotationAdd
BLAST
Nucleotide bindingi314 – 32512FADBy similarityAdd
BLAST
Nucleotide bindingi451 – 46111FADBy similarityAdd
BLAST
Nucleotide bindingi530 – 54819NADPBy similarityAdd
BLAST
Nucleotide bindingi625 – 64117NADPBy similarityAdd
BLAST

GO - Molecular functioni

  1. cytochrome-b5 reductase activity, acting on NAD(P)H Source: Ensembl
  2. electron carrier activity Source: Ensembl
  3. flavin adenine dinucleotide binding Source: Ensembl
  4. FMN binding Source: Ensembl
  5. hydrolase activity Source: Ensembl
  6. iron-cytochrome-c reductase activity Source: Ensembl
  7. iron ion binding Source: InterPro
  8. NADP binding Source: Ensembl
  9. NADPH-hemoprotein reductase activity Source: RefGenome
  10. nitric oxide dioxygenase activity Source: Ensembl
  11. oxidoreductase activity Source: MGI
  12. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen Source: RefGenome

GO - Biological processi

  1. carnitine metabolic process Source: Ensembl
  2. cellular organofluorine metabolic process Source: Ensembl
  3. cellular response to follicle-stimulating hormone stimulus Source: Ensembl
  4. cellular response to peptide hormone stimulus Source: Ensembl
  5. demethylation Source: Ensembl
  6. fatty acid oxidation Source: Ensembl
  7. flavonoid metabolic process Source: Ensembl
  8. internal peptidyl-lysine acetylation Source: Ensembl
  9. negative regulation of apoptotic process Source: Ensembl
  10. negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
  11. negative regulation of lipase activity Source: Ensembl
  12. nitrate catabolic process Source: Ensembl
  13. nitric oxide catabolic process Source: Ensembl
  14. positive regulation of cholesterol biosynthetic process Source: Ensembl
  15. positive regulation of chondrocyte differentiation Source: Ensembl
  16. positive regulation of monooxygenase activity Source: Ensembl
  17. positive regulation of smoothened signaling pathway Source: Ensembl
  18. positive regulation of steroid hormone biosynthetic process Source: Ensembl
  19. regulation of growth plate cartilage chondrocyte proliferation Source: Ensembl
  20. response to drug Source: Ensembl
  21. response to nutrient Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductase (EC:1.6.2.4)
Short name:
CPR
Short name:
P450R
Gene namesi
Name:Por
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:97744. Por.

Subcellular locationi

Endoplasmic reticulum membrane; Peripheral membrane protein
Note: Anchored to the ER membrane by its N-terminal hydrophobic region.

GO - Cellular componenti

  1. cytosol Source: RefGenome
  2. endoplasmic reticulum Source: UniProtKB-KW
  3. membrane Source: UniProtKB-KW
  4. mitochondrion Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 678677NADPH--cytochrome P450 reductasePRO_0000167597Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylglycineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP37040.
PaxDbiP37040.
PRIDEiP37040.

2D gel databases

SWISS-2DPAGEP37040.

PTM databases

PhosphoSiteiP37040.

Expressioni

Gene expression databases

BgeeiP37040.
CleanExiMM_POR.
ExpressionAtlasiP37040. baseline and differential.
GenevestigatoriP37040.

Interactioni

Protein-protein interaction databases

BioGridi202299. 1 interaction.
IntActiP37040. 6 interactions.
MINTiMINT-1863828.
STRINGi10090.ENSMUSP00000005651.

Structurei

3D structure databases

ProteinModelPortaliP37040.
SMRiP37040. Positions 63-678.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini80 – 224145Flavodoxin-likePROSITE-ProRule annotationAdd
BLAST
Domaini279 – 521243FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.Curated
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0369.
GeneTreeiENSGT00620000087711.
HOGENOMiHOG000282027.
HOVERGENiHBG000432.
InParanoidiP37040.
KOiK00327.
OMAiEFGAMEH.
OrthoDBiEOG7HQN7J.
PhylomeDBiP37040.
TreeFamiTF105719.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37040-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGDSHEDTSA TVPEAVAEEV SLFSTTDIVL FSLIVGVLTY WFIFKKKKEE
60 70 80 90 100
IPEFSKIQTT APPVKESSFV EKMKKTGRNI IVFYGSQTGT AEEFANRLSK
110 120 130 140 150
DAHRYGMRGM SADPEEYDLA DLSSLPEIDK SLVVFCMATY GEGDPTDNAQ
160 170 180 190 200
DFYDWLQETD VDLTGVKFAV FGLGNKTYEH FNAMGKYVDQ RLEQLGAQRI
210 220 230 240 250
FELGLGDDDG NLEEDFITWR EQFWPAVCEF FGVEATGEES SIRQYELVVH
260 270 280 290 300
EDMDTAKVYT GEMGRLKSYE NQKPPFDAKN PFLAAVTTNR KLNQGTERHL
310 320 330 340 350
MHLELDISDS KIRYESGDHV AVYPANDSTL VNQIGEILGA DLDVIMSLNN
360 370 380 390 400
LDEESNKKHP FPCPTTYRTA LTYYLDITNP PRTNVLYELA QYASEPSEQE
410 420 430 440 450
HLHKMASSSG EGKELYLSWV VEARRHILAI LQDYPSLRPP IDHLCELLPR
460 470 480 490 500
LQARYYSIAS SSKVHPNSVH ICAVAVEYEA KSGRVNKGVA TSWLRTKEPA
510 520 530 540 550
GENGRRALVP MFVRKSQFRL PFKPTTPVIM VGPGTGVAPF MGFIQERAWL
560 570 580 590 600
REQGKEVGET LLYYGCRRSD EDYLYREELA RFHKDGALTQ LNVAFSREQA
610 620 630 640 650
HKVYVQHLLK RDKEHLWKLI HEGGAHIYVC GDARNMAKDV QNTFYDIVAE
660 670
FGPMEHTQAV DYVKKLMTKG RYSLDVWS
Length:678
Mass (Da):77,044
Last modified:January 23, 2007 - v2
Checksum:i060282A7A55483AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17571 mRNA. Translation: BAA04496.1.
BC031463 mRNA. Translation: AAH31463.1.
CCDSiCCDS39318.1.
RefSeqiNP_032924.1. NM_008898.1.
XP_006504461.1. XM_006504398.1.
XP_006504462.1. XM_006504399.1.
UniGeneiMm.3863.

Genome annotation databases

EnsembliENSMUST00000005651; ENSMUSP00000005651; ENSMUSG00000005514.
GeneIDi18984.
KEGGimmu:18984.
UCSCiuc008zyt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17571 mRNA. Translation: BAA04496.1 .
BC031463 mRNA. Translation: AAH31463.1 .
CCDSi CCDS39318.1.
RefSeqi NP_032924.1. NM_008898.1.
XP_006504461.1. XM_006504398.1.
XP_006504462.1. XM_006504399.1.
UniGenei Mm.3863.

3D structure databases

ProteinModelPortali P37040.
SMRi P37040. Positions 63-678.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 202299. 1 interaction.
IntActi P37040. 6 interactions.
MINTi MINT-1863828.
STRINGi 10090.ENSMUSP00000005651.

PTM databases

PhosphoSitei P37040.

2D gel databases

SWISS-2DPAGE P37040.

Proteomic databases

MaxQBi P37040.
PaxDbi P37040.
PRIDEi P37040.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000005651 ; ENSMUSP00000005651 ; ENSMUSG00000005514 .
GeneIDi 18984.
KEGGi mmu:18984.
UCSCi uc008zyt.1. mouse.

Organism-specific databases

CTDi 5447.
MGIi MGI:97744. Por.

Phylogenomic databases

eggNOGi COG0369.
GeneTreei ENSGT00620000087711.
HOGENOMi HOG000282027.
HOVERGENi HBG000432.
InParanoidi P37040.
KOi K00327.
OMAi EFGAMEH.
OrthoDBi EOG7HQN7J.
PhylomeDBi P37040.
TreeFami TF105719.

Miscellaneous databases

ChiTaRSi Por. mouse.
NextBioi 295354.
PROi P37040.
SOURCEi Search...

Gene expression databases

Bgeei P37040.
CleanExi MM_POR.
ExpressionAtlasi P37040. baseline and differential.
Genevestigatori P37040.

Family and domain databases

Gene3Di 1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProi IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF000208. P450R. 1 hit.
PRINTSi PR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMi SSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEi PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse NADPH-cytochrome P-450 oxidoreductase: molecular cloning and functional expression in yeast."
    Ohgiya S., Ishizaki K., Kamataki T., Shinriki N.
    Biochim. Biophys. Acta 1186:137-141(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ddY.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.

Entry informationi

Entry nameiNCPR_MOUSE
AccessioniPrimary (citable) accession number: P37040
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3