Reviewed, UniProtKB/Swiss-Prot P37040 (NCPR_MOUSE)

Names and origin

Protein names

Recommended name:
    NADPH--cytochrome P450 reductase
      Short name=CPR
      Short name=P450R
    EC=1.6.2.4

Gene names

Name:

Por

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	678 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
Catalytic activity	NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.
Cofactor	FAD. FMN.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Note: Anchored to the ER membrane by its N-terminal hydrophobic region.
Sequence similarities	In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Ligand	FAD FMN Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from direct assay. Source: MGI soluble fraction Inferred from direct assay. Source: MGI
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro NADPH-hemoprotein reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 678	677	NADPH--cytochrome P450 reductase		PRO_0000167597
Regions
	Domain	80 – 224	145	Flavodoxin-like
	Domain	279 – 521	243	FAD-binding FR-type
	Nucleotide binding	170 – 201	32	FMN By similarity
	Nucleotide binding	314 – 325	12	FAD By similarity
	Nucleotide binding	451 – 461	11	FAD By similarity
	Nucleotide binding	530 – 548	19	NADP By similarity
	Nucleotide binding	625 – 641	17	NADP By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylglycine By similarity
	Modified residue	575	1	Phosphotyrosine Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P37040-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 060282A7A55483AF	FASTA	678	77,044
	10 20 30 40 50 60 MGDSHEDTSA TVPEAVAEEV SLFSTTDIVL FSLIVGVLTY WFIFKKKKEE IPEFSKIQTT 70 80 90 100 110 120 APPVKESSFV EKMKKTGRNI IVFYGSQTGT AEEFANRLSK DAHRYGMRGM SADPEEYDLA 130 140 150 160 170 180 DLSSLPEIDK SLVVFCMATY GEGDPTDNAQ DFYDWLQETD VDLTGVKFAV FGLGNKTYEH 190 200 210 220 230 240 FNAMGKYVDQ RLEQLGAQRI FELGLGDDDG NLEEDFITWR EQFWPAVCEF FGVEATGEES 250 260 270 280 290 300 SIRQYELVVH EDMDTAKVYT GEMGRLKSYE NQKPPFDAKN PFLAAVTTNR KLNQGTERHL 310 320 330 340 350 360 MHLELDISDS KIRYESGDHV AVYPANDSTL VNQIGEILGA DLDVIMSLNN LDEESNKKHP 370 380 390 400 410 420 FPCPTTYRTA LTYYLDITNP PRTNVLYELA QYASEPSEQE HLHKMASSSG EGKELYLSWV 430 440 450 460 470 480 VEARRHILAI LQDYPSLRPP IDHLCELLPR LQARYYSIAS SSKVHPNSVH ICAVAVEYEA 490 500 510 520 530 540 KSGRVNKGVA TSWLRTKEPA GENGRRALVP MFVRKSQFRL PFKPTTPVIM VGPGTGVAPF 550 560 570 580 590 600 MGFIQERAWL REQGKEVGET LLYYGCRRSD EDYLYREELA RFHKDGALTQ LNVAFSREQA 610 620 630 640 650 660 HKVYVQHLLK RDKEHLWKLI HEGGAHIYVC GDARNMAKDV QNTFYDIVAE FGPMEHTQAV 670 DYVKKLMTKG RYSLDVWS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mouse NADPH-cytochrome P-450 oxidoreductase: molecular cloning and functional expression in yeast." Ohgiya S., Ishizaki K., Kamataki T., Shinriki N. Biochim. Biophys. Acta 1186:137-141(1994) [PubMed: 8011664] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ddY.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary gland.
[3]	"A differential phosphoproteomic analysis of retinoic acid-treated P19 cells." Smith J.C., Duchesne M.A., Tozzi P., Ethier M., Figeys D. J. Proteome Res. 6:3174-3186(2007) [PubMed: 17622165] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-575, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D17571 mRNA. Translation: BAA04496.1. BC031463 mRNA. Translation: AAH31463.1.
IPI	IPI00621548.
RefSeq	NP_032924.1.
UniGene	Mm.3863
3D structure databases
HSSP	HSSP built from PDB template 1AMO based on UniProtKB P00388.
SMR	P37040. Positions 63-678.
ModBase	Search...
Protein-protein interaction databases
IntAct	P37040. 1 interaction.
PTM databases
PhosphoSite	P37040.
2-D gel databases
SWISS-2DPAGE	P37040.
Genome annotation databases
Ensembl	ENSMUSG00000005514. Mus musculus. [Contig view]
GeneID	18984.
KEGG	mmu:18984.
Organism-specific databases
MGI	MGI:97744. Por.
Phylogenomic databases
HOGENOM	P37040.
HOVERGEN	P37040.
OMA	P37040. FHKDGAL.
Enzyme and pathway databases
BRENDA	1.6.2.4. 244.
Gene expression databases
Bgee	P37040.
CleanEx	MM_POR.
GermOnline	ENSMUSG00000005514. Mus musculus.
Family and domain databases
InterPro	IPR003097. FAD-binding_1. IPR017927. Fd_Rdtase_FAD-bd. IPR001094. Flavdoxin-like. IPR008254. Flavodoxin/NO_synth. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR015702. NADPH_Cyt_P450_Rdtase. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
PANTHER	PTHR19384:SF17. NADPH_Cyt_Red. 1 hit.
Pfam	PF00667. FAD_binding_1. 1 hit. PF00258. Flavodoxin_1. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00369. FLAVODOXIN. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. PS50902. FLAVODOXIN_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	295354.
SOURCE	Search...

Entry information

Entry name

NCPR_MOUSE

Accession

Primary (citable) accession number: P37040

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)