Reviewed, UniProtKB/Swiss-Prot P37039 (NCPR_CAVPO)

Names and origin

Protein names

Recommended name:
    NADPH--cytochrome P450 reductase
      Short name=CPR
      Short name=P450R
    EC=1.6.2.4

Gene names

Name:

POR

Organism

Cavia porcellus (Guinea pig)

Taxonomic identifier

10141 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	678 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
Catalytic activity	NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.
Cofactor	FAD. FMN.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Note: Anchored to the ER membrane by its N-terminal hydrophobic region.
Sequence similarities	In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain.
Caution	Was originally (Ref.1) thought to originate from mouse.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Ligand	FAD FMN Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro NADPH-hemoprotein reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 678	677	NADPH--cytochrome P450 reductase		PRO_0000167595
Regions
	Domain	80 – 224	145	Flavodoxin-like
	Domain	279 – 521	243	FAD-binding FR-type
	Nucleotide binding	170 – 201	32	FMN By similarity
	Nucleotide binding	314 – 325	12	FAD By similarity
	Nucleotide binding	451 – 461	11	FAD By similarity
	Nucleotide binding	530 – 548	19	NADP By similarity
	Nucleotide binding	625 – 641	17	NADP By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylglycine By similarity
	Modified residue	575	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P37039-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 4AC01B1825F0946F	FASTA	678	76,782
	10 20 30 40 50 60 MGDSHVDTGA TSTEAVAEEV SLFSMTDMIL LSVLVGFLTY FFLFRKKKEE IPEFTKISTT 70 80 90 100 110 120 TSSVKESSFV EKMKKTGRNI IVFYGSQTGT AEEFANRLSK DAHRYGMRGM AADPEEYDLA 130 140 150 160 170 180 DLSSLPEIDN SLAVFCMATY GEGDPTDNAQ DFYDWLQETD LDLSGVKYAV FGLGNKTYEH 190 200 210 220 230 240 FNSMGKYVDQ RLEQLGAQRI FELGLGDDDG NLEEDFITWR EQFWPAVCEF FGVEATGEES 250 260 270 280 290 300 SIRQYELVLH ADTDPAKVYT GEMGRLKSYE NQKPPFDAKN PFLAAVTTNR KLNQGTERHL 310 320 330 340 350 360 MHLELDISDS KIRYESGDHV AVYPANDSNL VNQLGKILGA DLDVVMSLKN LDEESNKKHP 370 380 390 400 410 420 FPCPTTYRTA LTYYLDITNP PRTNVLYELA QYASEPSEQE QLHKMASSSG EGKELYLTWV 430 440 450 460 470 480 VEARRHILAI LQDYPSLRPP IDHLCELLPR LQARYYSIAS SSKVHPNSVH ICAVVVEYET 490 500 510 520 530 540 KSGRINKGVA TSWLQAKDPA GENGRRALVP MFVRKSQFRL PFKSTTPVIM VGPGTGVAPF 550 560 570 580 590 600 IGFIQERAWL QQQGKEVGET LLYYGCRRSD EDYLYREELA QFHKDGTLTQ LNVAFSREQA 610 620 630 640 650 660 QKVYVQHLLK RDQEHLWKLI HEDGAHIYVC GDARNMARDV QNTFCNIAAE LGGMEHTQAV 670 DYVKKLMTKG RYSLDVWS

References

[1]	"Molecular cloning and sequence analysis of guinea-pig NADPH-cytochrome P-450 oxidoreductase." Ohgiya S., Goda T., Ishizaki K., Kamataki T., Shinriki N. Biochim. Biophys. Acta 1171:103-105(1992) [PubMed: 1420354] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Hartley.
[2]	Erratum Ohgiya S., Goda T., Ishizaki K., Kamataki T., Shinriki N. Biochim. Biophys. Acta 1174:313-313(1993) [PubMed: 8373812] [Abstract]

Cross-references

Sequence databases
EMBLGenBankDDBJ	D10498 mRNA. Translation: BAA01385.1.
PIR	S27158.
3D structure databases
HSSP	HSSP built from PDB template 1AMO based on UniProtKB P00388.
SMR	P37039. Positions 64-678.
ModBase	Search...
Protein-protein interaction databases
STRING	P37039.
Genome annotation databases
Ensembl	ENSCPOT00000006266; ENSCPOP00000005592; ENSCPOG00000006202; Cavia porcellus. [Genome view]
Phylogenomic databases
HOVERGEN	P37039.
Enzyme and pathway databases
BRENDA	1.6.2.4. 44.
Family and domain databases
InterPro	IPR003097. FAD-binding_1. IPR017927. Fd_Rdtase_FAD-bd. IPR001094. Flavdoxin-like. IPR008254. Flavodoxin/NO_synth. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR015702. NADPH_Cyt_P450_Rdtase. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
PANTHER	PTHR19384:SF17. NADPH_Cyt_Red. 1 hit.
Pfam	PF00667. FAD_binding_1. 1 hit. PF00258. Flavodoxin_1. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00369. FLAVODOXIN. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. PS50902. FLAVODOXIN_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCPR_CAVPO

Accession

Primary (citable) accession number: P37039

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	January 23, 2007
Last modified:	October 13, 2009
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)