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P37039 (NCPR_CAVPO) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADPH--cytochrome P450 reductase

Short name=CPR
Short name=P450R
EC=1.6.2.4
Gene names
Name:POR
OrganismCavia porcellus (Guinea pig) [Reference proteome]
Taxonomic identifier10141 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia

Protein attributes

Sequence length678 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.

Catalytic activity

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.

Cofactor

FAD.

FMN.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Note: Anchored to the ER membrane by its N-terminal hydrophobic region.

Sequence similarities

In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

Contains 1 FAD-binding FR-type domain.

Contains 1 flavodoxin-like domain.

Caution

Was originally (Ref.1) thought to originate from mouse.

Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
Membrane
   LigandFAD
Flavoprotein
FMN
NADP
   Molecular functionOxidoreductase
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionFMN binding

Inferred from electronic annotation. Source: InterPro

NADPH-hemoprotein reductase activity

Inferred from electronic annotation. Source: UniProtKB-EC

iron ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 678677NADPH--cytochrome P450 reductase
PRO_0000167595

Regions

Domain80 – 224145Flavodoxin-like
Domain279 – 521243FAD-binding FR-type
Nucleotide binding170 – 20132FMN By similarity
Nucleotide binding314 – 32512FAD By similarity
Nucleotide binding451 – 46111FAD By similarity
Nucleotide binding530 – 54819NADP By similarity
Nucleotide binding625 – 64117NADP By similarity

Amino acid modifications

Modified residue21N-acetylglycine By similarity

Sequences

Sequence LengthMass (Da)Tools
P37039 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 4AC01B1825F0946F

FASTA67876,782
        10         20         30         40         50         60 
MGDSHVDTGA TSTEAVAEEV SLFSMTDMIL LSVLVGFLTY FFLFRKKKEE IPEFTKISTT 

        70         80         90        100        110        120 
TSSVKESSFV EKMKKTGRNI IVFYGSQTGT AEEFANRLSK DAHRYGMRGM AADPEEYDLA 

       130        140        150        160        170        180 
DLSSLPEIDN SLAVFCMATY GEGDPTDNAQ DFYDWLQETD LDLSGVKYAV FGLGNKTYEH 

       190        200        210        220        230        240 
FNSMGKYVDQ RLEQLGAQRI FELGLGDDDG NLEEDFITWR EQFWPAVCEF FGVEATGEES 

       250        260        270        280        290        300 
SIRQYELVLH ADTDPAKVYT GEMGRLKSYE NQKPPFDAKN PFLAAVTTNR KLNQGTERHL 

       310        320        330        340        350        360 
MHLELDISDS KIRYESGDHV AVYPANDSNL VNQLGKILGA DLDVVMSLKN LDEESNKKHP 

       370        380        390        400        410        420 
FPCPTTYRTA LTYYLDITNP PRTNVLYELA QYASEPSEQE QLHKMASSSG EGKELYLTWV 

       430        440        450        460        470        480 
VEARRHILAI LQDYPSLRPP IDHLCELLPR LQARYYSIAS SSKVHPNSVH ICAVVVEYET 

       490        500        510        520        530        540 
KSGRINKGVA TSWLQAKDPA GENGRRALVP MFVRKSQFRL PFKSTTPVIM VGPGTGVAPF 

       550        560        570        580        590        600 
IGFIQERAWL QQQGKEVGET LLYYGCRRSD EDYLYREELA QFHKDGTLTQ LNVAFSREQA 

       610        620        630        640        650        660 
QKVYVQHLLK RDQEHLWKLI HEDGAHIYVC GDARNMARDV QNTFCNIAAE LGGMEHTQAV 

       670 
DYVKKLMTKG RYSLDVWS 

« Hide

References

[1]"Molecular cloning and sequence analysis of guinea-pig NADPH-cytochrome P-450 oxidoreductase."
Ohgiya S., Goda T., Ishizaki K., Kamataki T., Shinriki N.
Biochim. Biophys. Acta 1171:103-105(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Hartley.
[2]Erratum
Ohgiya S., Goda T., Ishizaki K., Kamataki T., Shinriki N.
Biochim. Biophys. Acta 1174:313-313(1993) [PubMed] [Europe PMC] [Abstract]

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D10498 mRNA. Translation: BAA01385.1.
PIRS27158.
RefSeqNP_001166473.1. NM_001173002.1.

3D structure databases

ProteinModelPortalP37039.
SMRP37039. Positions 64-678.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10141.ENSCPOP00000005592.

Proteomic databases

PRIDEP37039.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100135602.

Organism-specific databases

CTD5447.

Phylogenomic databases

eggNOGCOG0369.
HOGENOMHOG000282027.
HOVERGENHBG000432.
InParanoidP37039.

Family and domain databases

Gene3D1.20.990.10. 1 hit.
InterProIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFPIRSF000208. P450R. 1 hit.
PRINTSPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMSSF63380. SSF63380. 1 hit.
PROSITEPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNCPR_CAVPO
AccessionPrimary (citable) accession number: P37039
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: November 13, 2013
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families