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Protein

NADPH--cytochrome P450 reductase

Gene

Por

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.UniRule annotation

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • FADUniRule annotationNote: Binds 1 FAD per monomer.UniRule annotation
  • FMNUniRule annotationNote: Binds 1 FMN per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei208FMNUniRule annotation1
Binding sitei298NADPUniRule annotation1
Binding sitei424FADUniRule annotation1
Binding sitei478FADUniRule annotation1
Binding sitei535NADPUniRule annotation1
Binding sitei639NADPUniRule annotation1
Binding sitei677FADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi86 – 91FMNUniRule annotation6
Nucleotide bindingi138 – 141FMNUniRule annotation4
Nucleotide bindingi173 – 182FMNUniRule annotation10
Nucleotide bindingi454 – 457FADUniRule annotation4
Nucleotide bindingi472 – 474FADUniRule annotation3
Nucleotide bindingi488 – 491FADUniRule annotation4
Nucleotide bindingi596 – 597NADPUniRule annotation2
Nucleotide bindingi602 – 606NADPUniRule annotation5

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductaseUniRule annotation (EC:1.6.2.4UniRule annotation)
Short name:
CPRUniRule annotation
Short name:
P450RUniRule annotation
Gene namesi
Name:PorUniRule annotation
OrganismiCavia porcellus (Guinea pig)
Taxonomic identifieri10141 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
Proteomesi
  • UP000005447 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Endoplasmic reticulum membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 21LumenalUniRule annotationAdd BLAST20
Transmembranei22 – 42HelicalUniRule annotationAdd BLAST21
Topological domaini43 – 678CytoplasmicUniRule annotationAdd BLAST636

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001675952 – 678NADPH--cytochrome P450 reductaseAdd BLAST677

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylglycineBy similarity1
Modified residuei63PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiP37039.

Interactioni

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000005592.

Structurei

3D structure databases

ProteinModelPortaliP37039.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini80 – 224Flavodoxin-likeUniRule annotationAdd BLAST145
Domaini279 – 521FAD-binding FR-typeUniRule annotationAdd BLAST243

Sequence similaritiesi

Belongs to the NADPH--cytochrome P450 reductase family.UniRule annotation
In the N-terminal section; belongs to the flavodoxin family.UniRule annotation
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation
Contains 1 FAD-binding FR-type domain.UniRule annotation
Contains 1 flavodoxin-like domain.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1158. Eukaryota.
COG0369. LUCA.
HOGENOMiHOG000282027.
HOVERGENiHBG000432.
InParanoidiP37039.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03212. NCPR. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37039-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDSHVDTGA TSTEAVAEEV SLFSMTDMIL LSVLVGFLTY FFLFRKKKEE
60 70 80 90 100
IPEFTKISTT TSSVKESSFV EKMKKTGRNI IVFYGSQTGT AEEFANRLSK
110 120 130 140 150
DAHRYGMRGM AADPEEYDLA DLSSLPEIDN SLAVFCMATY GEGDPTDNAQ
160 170 180 190 200
DFYDWLQETD LDLSGVKYAV FGLGNKTYEH FNSMGKYVDQ RLEQLGAQRI
210 220 230 240 250
FELGLGDDDG NLEEDFITWR EQFWPAVCEF FGVEATGEES SIRQYELVLH
260 270 280 290 300
ADTDPAKVYT GEMGRLKSYE NQKPPFDAKN PFLAAVTTNR KLNQGTERHL
310 320 330 340 350
MHLELDISDS KIRYESGDHV AVYPANDSNL VNQLGKILGA DLDVVMSLKN
360 370 380 390 400
LDEESNKKHP FPCPTTYRTA LTYYLDITNP PRTNVLYELA QYASEPSEQE
410 420 430 440 450
QLHKMASSSG EGKELYLTWV VEARRHILAI LQDYPSLRPP IDHLCELLPR
460 470 480 490 500
LQARYYSIAS SSKVHPNSVH ICAVVVEYET KSGRINKGVA TSWLQAKDPA
510 520 530 540 550
GENGRRALVP MFVRKSQFRL PFKSTTPVIM VGPGTGVAPF IGFIQERAWL
560 570 580 590 600
QQQGKEVGET LLYYGCRRSD EDYLYREELA QFHKDGTLTQ LNVAFSREQA
610 620 630 640 650
QKVYVQHLLK RDQEHLWKLI HEDGAHIYVC GDARNMARDV QNTFCNIAAE
660 670
LGGMEHTQAV DYVKKLMTKG RYSLDVWS
Length:678
Mass (Da):76,782
Last modified:January 23, 2007 - v2
Checksum:i4AC01B1825F0946F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10498 mRNA. Translation: BAA01385.1.
PIRiS27158.
RefSeqiNP_001166473.1. NM_001173002.1.

Genome annotation databases

GeneIDi100135602.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10498 mRNA. Translation: BAA01385.1.
PIRiS27158.
RefSeqiNP_001166473.1. NM_001173002.1.

3D structure databases

ProteinModelPortaliP37039.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000005592.

Proteomic databases

PRIDEiP37039.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100135602.

Organism-specific databases

CTDi5447.

Phylogenomic databases

eggNOGiKOG1158. Eukaryota.
COG0369. LUCA.
HOGENOMiHOG000282027.
HOVERGENiHBG000432.
InParanoidiP37039.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03212. NCPR. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNCPR_CAVPO
AccessioniPrimary (citable) accession number: P37039
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: October 5, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to originate from mouse.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.