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Protein

Vitamin B12-binding protein

Gene

btuF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC.UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei50CobalaminUniRule annotation1 Publication1
Sitei72Important for BtuC bindingUniRule annotation1 Publication1
Sitei202Important for BtuC bindingUniRule annotation1 Publication1

GO - Molecular functioni

  • cobalamin binding Source: EcoliWiki

GO - Biological processi

  • cobalamin transport Source: EcoliWiki
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG12334-MONOMER.
ECOL316407:JW0154-MONOMER.
MetaCyc:EG12334-MONOMER.
BRENDAi3.6.3.33. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Vitamin B12-binding proteinUniRule annotation
Gene namesi
Name:btuF
Synonyms:yadT
Ordered Locus Names:b0158, JW0154
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12334. btuF.

Subcellular locationi

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc
  • periplasmic space Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 221 PublicationAdd BLAST22
ChainiPRO_000000349723 – 266Vitamin B12-binding proteinAdd BLAST244

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi183 ↔ 259

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP37028.
PRIDEiP37028.

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (BtuD), two transmembrane proteins (BtuC) and a solute-binding protein (BtuF).UniRule annotation2 Publications

Protein-protein interaction databases

BioGridi4261861. 275 interactors.
DIPiDIP-11194N.
IntActiP37028. 5 interactors.
MINTiMINT-6803779.
STRINGi511145.b0158.

Structurei

Secondary structure

1266
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi26 – 28Combined sources3
Helixi31 – 39Combined sources9
Beta strandi45 – 48Combined sources4
Helixi55 – 59Combined sources5
Beta strandi62 – 65Combined sources4
Helixi71 – 76Combined sources6
Beta strandi80 – 84Combined sources5
Turni86 – 88Combined sources3
Helixi91 – 99Combined sources9
Beta strandi104 – 106Combined sources3
Helixi112 – 122Combined sources11
Helixi123 – 125Combined sources3
Beta strandi126 – 128Combined sources3
Helixi129 – 150Combined sources22
Beta strandi156 – 160Combined sources5
Beta strandi164 – 166Combined sources3
Beta strandi172 – 174Combined sources3
Helixi175 – 182Combined sources8
Beta strandi185 – 187Combined sources3
Turni188 – 191Combined sources4
Beta strandi193 – 196Combined sources4
Helixi201 – 205Combined sources5
Beta strandi210 – 216Combined sources7
Helixi218 – 220Combined sources3
Helixi221 – 228Combined sources8
Helixi229 – 231Combined sources3
Beta strandi236 – 239Combined sources4
Helixi241 – 245Combined sources5
Beta strandi246 – 248Combined sources3
Helixi251 – 262Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1N2ZX-ray2.00A/B22-266[»]
1N4AX-ray2.00A/B23-266[»]
1N4DX-ray3.00A/B23-266[»]
2QI9X-ray2.60F22-266[»]
4DBLX-ray3.49E/J22-266[»]
4FI3X-ray3.47F22-266[»]
ProteinModelPortaliP37028.
SMRiP37028.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37028.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 266Fe/B12 periplasmic-bindingUniRule annotationAdd BLAST242

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni242 – 246Cobalamin-bindingUniRule annotation2 Publications5

Sequence similaritiesi

Belongs to the BtuF family.UniRule annotationCurated
Contains 1 Fe/B12 periplasmic-binding domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105JKQ. Bacteria.
COG0614. LUCA.
HOGENOMiHOG000282913.
InParanoidiP37028.
KOiK06858.
OMAiVSVFYQV.
PhylomeDBiP37028.

Family and domain databases

HAMAPiMF_01000. BtuF. 1 hit.
InterProiIPR002491. ABC_transptr_periplasmic_BD.
IPR023544. ABC_transptr_vit_B12-bd.
[Graphical view]
PfamiPF01497. Peripla_BP_2. 1 hit.
[Graphical view]
PROSITEiPS50983. FE_B12_PBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37028-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKSLFRALV ALSFLAPLWL NAAPRVITLS PANTELAFAA GITPVGVSSY
60 70 80 90 100
SDYPPQAQKI EQVSTWQGMN LERIVALKPD LVIAWRGGNA ERQVDQLASL
110 120 130 140 150
GIKVMWVDAT SIEQIANALR QLAPWSPQPD KAEQAAQSLL DQYAQLKAQY
160 170 180 190 200
ADKPKKRVFL QFGINPPFTS GKESIQNQVL EVCGGENIFK DSRVPWPQVS
210 220 230 240 250
REQVLARSPQ AIVITGGPDQ IPKIKQYWGE QLKIPVIPLT SDWFERASPR
260
IILAAQQLCN ALSQVD
Length:266
Mass (Da):29,367
Last modified:November 1, 1997 - v2
Checksum:i480F2E620ACD6EA1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti208S → L (PubMed:8202364).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70214 Genomic DNA. Translation: AAB08588.1.
U00096 Genomic DNA. Translation: AAC73269.1.
AP009048 Genomic DNA. Translation: BAB96735.2.
PIRiF64739.
RefSeqiNP_414700.1. NC_000913.3.
WP_001129927.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73269; AAC73269; b0158.
BAB96735; BAB96735; BAB96735.
GeneIDi947574.
KEGGiecj:JW0154.
eco:b0158.
PATRICi32115425. VBIEscCol129921_0164.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70214 Genomic DNA. Translation: AAB08588.1.
U00096 Genomic DNA. Translation: AAC73269.1.
AP009048 Genomic DNA. Translation: BAB96735.2.
PIRiF64739.
RefSeqiNP_414700.1. NC_000913.3.
WP_001129927.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1N2ZX-ray2.00A/B22-266[»]
1N4AX-ray2.00A/B23-266[»]
1N4DX-ray3.00A/B23-266[»]
2QI9X-ray2.60F22-266[»]
4DBLX-ray3.49E/J22-266[»]
4FI3X-ray3.47F22-266[»]
ProteinModelPortaliP37028.
SMRiP37028.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261861. 275 interactors.
DIPiDIP-11194N.
IntActiP37028. 5 interactors.
MINTiMINT-6803779.
STRINGi511145.b0158.

Proteomic databases

PaxDbiP37028.
PRIDEiP37028.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73269; AAC73269; b0158.
BAB96735; BAB96735; BAB96735.
GeneIDi947574.
KEGGiecj:JW0154.
eco:b0158.
PATRICi32115425. VBIEscCol129921_0164.

Organism-specific databases

EchoBASEiEB2238.
EcoGeneiEG12334. btuF.

Phylogenomic databases

eggNOGiENOG4105JKQ. Bacteria.
COG0614. LUCA.
HOGENOMiHOG000282913.
InParanoidiP37028.
KOiK06858.
OMAiVSVFYQV.
PhylomeDBiP37028.

Enzyme and pathway databases

BioCyciEcoCyc:EG12334-MONOMER.
ECOL316407:JW0154-MONOMER.
MetaCyc:EG12334-MONOMER.
BRENDAi3.6.3.33. 2026.

Miscellaneous databases

EvolutionaryTraceiP37028.
PROiP37028.

Family and domain databases

HAMAPiMF_01000. BtuF. 1 hit.
InterProiIPR002491. ABC_transptr_periplasmic_BD.
IPR023544. ABC_transptr_vit_B12-bd.
[Graphical view]
PfamiPF01497. Peripla_BP_2. 1 hit.
[Graphical view]
PROSITEiPS50983. FE_B12_PBP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBTUF_ECOLI
AccessioniPrimary (citable) accession number: P37028
Secondary accession number(s): P77436
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Vitamin B12 is bound in a deep cleft formed at the interface between the two lobes of BtuF.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.