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P37025

- LIGT_ECOLI

UniProt

P37025 - LIGT_ECOLI

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Protein

2'-5'-RNA ligase

Gene
ligT, yadP, b0147, JW5011
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Specifically ligates tRNA half-molecules containing nucleoside base modifications and shows a preference among different tRNA species. Acts in the absence of ATP to form a 2'-5' phosphodiester linkage.

GO - Molecular functioni

  1. 2'-5'-RNA ligase activity Source: EcoCyc

GO - Biological processi

  1. RNA metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Enzyme and pathway databases

BioCyciEcoCyc:EG12330-MONOMER.
ECOL316407:JW5011-MONOMER.
MetaCyc:EG12330-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
2'-5'-RNA ligase (EC:6.5.1.-)
Gene namesi
Name:ligT
Synonyms:yadP
Ordered Locus Names:b0147, JW5011
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG12330. ligT.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 1761752'-5'-RNA ligasePRO_0000084421Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP37025.

Interactioni

Protein-protein interaction databases

STRINGi511145.b0147.

Structurei

3D structure databases

ProteinModelPortaliP37025.
SMRiP37025. Positions 5-175.

Family & Domainsi

Phylogenomic databases

eggNOGiCOG1514.
HOGENOMiHOG000226389.
KOiK01975.
OMAiDAGHWPR.
OrthoDBiEOG632D2K.
PhylomeDBiP37025.

Family and domain databases

Gene3Di3.90.1140.10. 1 hit.
InterProiIPR004175. 2_5_ligase.
IPR014051. Phosphoesterase_HXTX.
IPR009097. RNA_ligase/cNuc_Pdiesterase.
[Graphical view]
PfamiPF02834. LigT_PEase. 2 hits.
[Graphical view]
SUPFAMiSSF55144. SSF55144. 1 hit.
TIGRFAMsiTIGR02258. 2_5_ligase. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37025-1 [UniParc]FASTAAdd to Basket

« Hide

MSEPQRLFFA IDLPAEIREQ IIHWRATHFP PEAGRPVAAD NLHLTLAFLG    50
EVSAEKEKAL SLLAGRIRQP GFTLTLDDAG QWLRSRVVWL GMRQPPRGLI 100
QLANMLRSQA ARSGCFQSNR PFHPHITLLR DASEAVTIPP PGFNWSYAVT 150
EFTLYASSFA RGRTRYTPLK RWALTQ 176
Length:176
Mass (Da):19,934
Last modified:January 23, 2007 - v3
Checksum:i9DAA9FB3CE4F777E
GO

Sequence cautioni

The sequence M60726 differs from that shown. Reason: Frameshift at position 168.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti174 – 1741L → V in M60726. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC73258.2.
AP009048 Genomic DNA. Translation: BAB96724.2.
M60726 Genomic DNA. No translation available.
PIRiC64738.
RefSeqiNP_414689.4. NC_000913.3.
YP_488450.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73258; AAC73258; b0147.
BAB96724; BAB96724; BAB96724.
GeneIDi12930750.
944848.
KEGGiecj:Y75_p0144.
eco:b0147.
PATRICi32115401. VBIEscCol129921_0152.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC73258.2 .
AP009048 Genomic DNA. Translation: BAB96724.2 .
M60726 Genomic DNA. No translation available.
PIRi C64738.
RefSeqi NP_414689.4. NC_000913.3.
YP_488450.1. NC_007779.1.

3D structure databases

ProteinModelPortali P37025.
SMRi P37025. Positions 5-175.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 511145.b0147.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73258 ; AAC73258 ; b0147 .
BAB96724 ; BAB96724 ; BAB96724 .
GeneIDi 12930750.
944848.
KEGGi ecj:Y75_p0144.
eco:b0147.
PATRICi 32115401. VBIEscCol129921_0152.

Organism-specific databases

EchoBASEi EB2234.
EcoGenei EG12330. ligT.

Phylogenomic databases

eggNOGi COG1514.
HOGENOMi HOG000226389.
KOi K01975.
OMAi DAGHWPR.
OrthoDBi EOG632D2K.
PhylomeDBi P37025.

Enzyme and pathway databases

BioCyci EcoCyc:EG12330-MONOMER.
ECOL316407:JW5011-MONOMER.
MetaCyc:EG12330-MONOMER.

Miscellaneous databases

PROi P37025.

Gene expression databases

Genevestigatori P37025.

Family and domain databases

Gene3Di 3.90.1140.10. 1 hit.
InterProi IPR004175. 2_5_ligase.
IPR014051. Phosphoesterase_HXTX.
IPR009097. RNA_ligase/cNuc_Pdiesterase.
[Graphical view ]
Pfami PF02834. LigT_PEase. 2 hits.
[Graphical view ]
SUPFAMi SSF55144. SSF55144. 1 hit.
TIGRFAMsi TIGR02258. 2_5_ligase. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region."
    Fujita N., Mori H., Yura T., Ishihama A.
    Nucleic Acids Res. 22:1637-1639(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Nucleotide sequence and characterization of the sfs1 gene: sfs1 is involved in CRP*-dependent mal gene expression in Escherichia coli."
    Kawamukai M., Utsumi R., Takeda K., Higashi A., Matsuda H., Choi Y.-L., Komano T.
    J. Bacteriol. 173:2644-2648(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 107-176.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "The 2'-5' RNA ligase of Escherichia coli. Purification, cloning, and genomic disruption."
    Arn E.A., Abelson J.N.
    J. Biol. Chem. 271:31145-31153(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-23, CHARACTERIZATION.

Entry informationi

Entry nameiLIGT_ECOLI
AccessioniPrimary (citable) accession number: P37025
Secondary accession number(s): Q8KJQ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 98 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

External Data

Dasty 3

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