Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

RNA 2',3'-cyclic phosphodiesterase

Gene

thpR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'-phosphomonoester (PubMed:25239919). In vitro, can also ligate 5' and 3' half-tRNA molecules with 2',3'-cyclic phosphate and 5'-hydroxyl termini, respectively, to the product containing the 2'-5' phosphodiester linkage. This reaction does not require ATP and is reversible (PubMed:6347395 and PubMed:8940112).3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei43Proton donorUniRule annotation1 Publication1
Active sitei125Proton acceptorUniRule annotation1 Publication1

GO - Molecular functioni

  • 2',3'-cyclic-nucleotide 3'-phosphodiesterase activity Source: EcoCyc
  • 2'-5'-RNA ligase activity Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:EG12330-MONOMER.
ECOL316407:JW5011-MONOMER.
MetaCyc:EG12330-MONOMER.
BRENDAi6.5.1.B1. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 2',3'-cyclic phosphodiesterase1 PublicationUniRule annotation (EC:3.1.4.-UniRule annotation1 Publication)
Short name:
RNA 2',3'-CPDase1 PublicationUniRule annotation
Alternative name(s):
Two-histidine 2',3'-cyclic phosphodiesterase acting on RNA1 Publication
Gene namesi
Name:thpR1 PublicationUniRule annotation
Synonyms:ligTImported, yadP
Ordered Locus Names:b0147, JW5011
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12330. thpR.

Pathology & Biotechi

Disruption phenotypei

Disruption abolishes ligase activity in cell lysates, but mutants grow normally under laboratory conditions.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi43H → A: Lack of CPDase activity. 1 Publication1
Mutagenesisi45T → A: Lack of CPDase activity. 1 Publication1
Mutagenesisi48F → A: Decreases CPDase activity. 1 Publication1
Mutagenesisi125H → A: Lack of CPDase activity. 1 Publication1
Mutagenesisi127T → A: Does not affect CPDase activity. 1 Publication1
Mutagenesisi130R → A: Lack of CPDase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000844212 – 176RNA 2',3'-cyclic phosphodiesteraseAdd BLAST175

Proteomic databases

PaxDbiP37025.
PRIDEiP37025.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4259744. 107 interactors.
STRINGi511145.b0147.

Structurei

Secondary structure

1176
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 11Combined sources7
Helixi15 – 28Combined sources14
Helixi31 – 33Combined sources3
Helixi39 – 41Combined sources3
Beta strandi44 – 52Combined sources9
Helixi54 – 64Combined sources11
Beta strandi72 – 75Combined sources4
Beta strandi78 – 82Combined sources5
Turni83 – 86Combined sources4
Beta strandi87 – 91Combined sources5
Helixi97 – 112Combined sources16
Beta strandi126 – 131Combined sources6
Beta strandi146 – 149Combined sources4
Beta strandi151 – 159Combined sources9
Beta strandi161 – 173Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4QAKX-ray2.02A/B1-176[»]
ProteinModelPortaliP37025.
SMRiP37025.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi43 – 46HXTX 1UniRule annotationCurated4
Motifi125 – 128HXTX 2UniRule annotationCurated4

Sequence similaritiesi

Belongs to the 2H phosphoesterase superfamily. ThpR family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4107XID. Bacteria.
COG1514. LUCA.
HOGENOMiHOG000226389.
InParanoidiP37025.
KOiK01975.
OMAiGVVWLGC.
PhylomeDBiP37025.

Family and domain databases

Gene3Di3.90.1140.10. 1 hit.
HAMAPiMF_01940. RNA_CPDase. 1 hit.
InterProiIPR014051. Phosphoesterase_HXTX.
IPR004175. RNA_CPDase.
IPR009097. RNA_ligase/cNuc_Pdiesterase.
[Graphical view]
PfamiPF02834. LigT_PEase. 2 hits.
[Graphical view]
SUPFAMiSSF55144. SSF55144. 1 hit.
TIGRFAMsiTIGR02258. 2_5_ligase. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37025-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEPQRLFFA IDLPAEIREQ IIHWRATHFP PEAGRPVAAD NLHLTLAFLG
60 70 80 90 100
EVSAEKEKAL SLLAGRIRQP GFTLTLDDAG QWLRSRVVWL GMRQPPRGLI
110 120 130 140 150
QLANMLRSQA ARSGCFQSNR PFHPHITLLR DASEAVTIPP PGFNWSYAVT
160 170
EFTLYASSFA RGRTRYTPLK RWALTQ
Length:176
Mass (Da):19,934
Last modified:January 23, 2007 - v3
Checksum:i9DAA9FB3CE4F777E
GO

Sequence cautioni

The sequence M60726 differs from that shown. Reason: Frameshift at position 168.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti174L → V in M60726 (PubMed:2013578).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73258.2.
AP009048 Genomic DNA. Translation: BAB96724.2.
M60726 Genomic DNA. No translation available.
PIRiC64738.
RefSeqiNP_414689.4. NC_000913.3.
WP_001294700.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73258; AAC73258; b0147.
BAB96724; BAB96724; BAB96724.
GeneIDi944848.
KEGGiecj:JW5011.
eco:b0147.
PATRICi32115401. VBIEscCol129921_0152.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73258.2.
AP009048 Genomic DNA. Translation: BAB96724.2.
M60726 Genomic DNA. No translation available.
PIRiC64738.
RefSeqiNP_414689.4. NC_000913.3.
WP_001294700.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4QAKX-ray2.02A/B1-176[»]
ProteinModelPortaliP37025.
SMRiP37025.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259744. 107 interactors.
STRINGi511145.b0147.

Proteomic databases

PaxDbiP37025.
PRIDEiP37025.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73258; AAC73258; b0147.
BAB96724; BAB96724; BAB96724.
GeneIDi944848.
KEGGiecj:JW5011.
eco:b0147.
PATRICi32115401. VBIEscCol129921_0152.

Organism-specific databases

EchoBASEiEB2234.
EcoGeneiEG12330. thpR.

Phylogenomic databases

eggNOGiENOG4107XID. Bacteria.
COG1514. LUCA.
HOGENOMiHOG000226389.
InParanoidiP37025.
KOiK01975.
OMAiGVVWLGC.
PhylomeDBiP37025.

Enzyme and pathway databases

BioCyciEcoCyc:EG12330-MONOMER.
ECOL316407:JW5011-MONOMER.
MetaCyc:EG12330-MONOMER.
BRENDAi6.5.1.B1. 2026.

Miscellaneous databases

PROiP37025.

Family and domain databases

Gene3Di3.90.1140.10. 1 hit.
HAMAPiMF_01940. RNA_CPDase. 1 hit.
InterProiIPR014051. Phosphoesterase_HXTX.
IPR004175. RNA_CPDase.
IPR009097. RNA_ligase/cNuc_Pdiesterase.
[Graphical view]
PfamiPF02834. LigT_PEase. 2 hits.
[Graphical view]
SUPFAMiSSF55144. SSF55144. 1 hit.
TIGRFAMsiTIGR02258. 2_5_ligase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHPR_ECOLI
AccessioniPrimary (citable) accession number: P37025
Secondary accession number(s): Q8KJQ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be a 2',5'-RNA ligase.2 Publications

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.