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P37025

- LIGT_ECOLI

UniProt

P37025 - LIGT_ECOLI

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Protein

2'-5'-RNA ligase

Gene

ligT

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Specifically ligates tRNA half-molecules containing nucleoside base modifications and shows a preference among different tRNA species. Acts in the absence of ATP to form a 2'-5' phosphodiester linkage.

GO - Molecular functioni

  1. 2'-5'-RNA ligase activity Source: EcoCyc

GO - Biological processi

  1. RNA metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Enzyme and pathway databases

BioCyciEcoCyc:EG12330-MONOMER.
ECOL316407:JW5011-MONOMER.
MetaCyc:EG12330-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
2'-5'-RNA ligase (EC:6.5.1.-)
Gene namesi
Name:ligT
Synonyms:yadP
Ordered Locus Names:b0147, JW5011
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG12330. ligT.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 1761752'-5'-RNA ligasePRO_0000084421Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP37025.

Interactioni

Protein-protein interaction databases

STRINGi511145.b0147.

Structurei

3D structure databases

ProteinModelPortaliP37025.
SMRiP37025. Positions 5-175.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiCOG1514.
HOGENOMiHOG000226389.
InParanoidiP37025.
KOiK01975.
OMAiDAGHWPR.
OrthoDBiEOG632D2K.
PhylomeDBiP37025.

Family and domain databases

Gene3Di3.90.1140.10. 1 hit.
InterProiIPR004175. 2_5_ligase.
IPR014051. Phosphoesterase_HXTX.
IPR009097. RNA_ligase/cNuc_Pdiesterase.
[Graphical view]
PfamiPF02834. LigT_PEase. 2 hits.
[Graphical view]
SUPFAMiSSF55144. SSF55144. 1 hit.
TIGRFAMsiTIGR02258. 2_5_ligase. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37025-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSEPQRLFFA IDLPAEIREQ IIHWRATHFP PEAGRPVAAD NLHLTLAFLG
60 70 80 90 100
EVSAEKEKAL SLLAGRIRQP GFTLTLDDAG QWLRSRVVWL GMRQPPRGLI
110 120 130 140 150
QLANMLRSQA ARSGCFQSNR PFHPHITLLR DASEAVTIPP PGFNWSYAVT
160 170
EFTLYASSFA RGRTRYTPLK RWALTQ
Length:176
Mass (Da):19,934
Last modified:January 23, 2007 - v3
Checksum:i9DAA9FB3CE4F777E
GO

Sequence cautioni

The sequence M60726 differs from that shown. Reason: Frameshift at position 168.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti174 – 1741L → V in M60726. (PubMed:2013578)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC73258.2.
AP009048 Genomic DNA. Translation: BAB96724.2.
M60726 Genomic DNA. No translation available.
PIRiC64738.
RefSeqiNP_414689.4. NC_000913.3.
YP_488450.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73258; AAC73258; b0147.
BAB96724; BAB96724; BAB96724.
GeneIDi12930750.
944848.
KEGGiecj:Y75_p0144.
eco:b0147.
PATRICi32115401. VBIEscCol129921_0152.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC73258.2 .
AP009048 Genomic DNA. Translation: BAB96724.2 .
M60726 Genomic DNA. No translation available.
PIRi C64738.
RefSeqi NP_414689.4. NC_000913.3.
YP_488450.1. NC_007779.1.

3D structure databases

ProteinModelPortali P37025.
SMRi P37025. Positions 5-175.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 511145.b0147.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73258 ; AAC73258 ; b0147 .
BAB96724 ; BAB96724 ; BAB96724 .
GeneIDi 12930750.
944848.
KEGGi ecj:Y75_p0144.
eco:b0147.
PATRICi 32115401. VBIEscCol129921_0152.

Organism-specific databases

EchoBASEi EB2234.
EcoGenei EG12330. ligT.

Phylogenomic databases

eggNOGi COG1514.
HOGENOMi HOG000226389.
InParanoidi P37025.
KOi K01975.
OMAi DAGHWPR.
OrthoDBi EOG632D2K.
PhylomeDBi P37025.

Enzyme and pathway databases

BioCyci EcoCyc:EG12330-MONOMER.
ECOL316407:JW5011-MONOMER.
MetaCyc:EG12330-MONOMER.

Miscellaneous databases

PROi P37025.

Gene expression databases

Genevestigatori P37025.

Family and domain databases

Gene3Di 3.90.1140.10. 1 hit.
InterProi IPR004175. 2_5_ligase.
IPR014051. Phosphoesterase_HXTX.
IPR009097. RNA_ligase/cNuc_Pdiesterase.
[Graphical view ]
Pfami PF02834. LigT_PEase. 2 hits.
[Graphical view ]
SUPFAMi SSF55144. SSF55144. 1 hit.
TIGRFAMsi TIGR02258. 2_5_ligase. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region."
    Fujita N., Mori H., Yura T., Ishihama A.
    Nucleic Acids Res. 22:1637-1639(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Nucleotide sequence and characterization of the sfs1 gene: sfs1 is involved in CRP*-dependent mal gene expression in Escherichia coli."
    Kawamukai M., Utsumi R., Takeda K., Higashi A., Matsuda H., Choi Y.-L., Komano T.
    J. Bacteriol. 173:2644-2648(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 107-176.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "The 2'-5' RNA ligase of Escherichia coli. Purification, cloning, and genomic disruption."
    Arn E.A., Abelson J.N.
    J. Biol. Chem. 271:31145-31153(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-23, CHARACTERIZATION.

Entry informationi

Entry nameiLIGT_ECOLI
AccessioniPrimary (citable) accession number: P37025
Secondary accession number(s): Q8KJQ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 100 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

External Data

Dasty 3