P37025 (LIGT_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2'-5'-RNA ligase
EC=6.5.1.-

Gene names

Name:	ligT
Synonyms:	yadP
Ordered Locus Names:	b0147, JW5011

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	176 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Specifically ligates tRNA half-molecules containing nucleoside base modifications and shows a preference among different tRNA species. Acts in the absence of ATP to form a 2'-5' phosphodiester linkage.
Sequence caution	The sequence M60726 differs from that shown. Reason: Frameshift at position 168.

Ontologies

Keywords
Molecular function	Ligase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	RNA metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	2'-5'-RNA ligase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.5
	Chain	2 – 176	175	2'-5'-RNA ligase		PRO_0000084421
Experimental info
	Sequence conflict	174	1	L → V in M60726. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P37025 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 9DAA9FB3CE4F777E	FASTA	176	19,934
	10 20 30 40 50 60 MSEPQRLFFA IDLPAEIREQ IIHWRATHFP PEAGRPVAAD NLHLTLAFLG EVSAEKEKAL 70 80 90 100 110 120 SLLAGRIRQP GFTLTLDDAG QWLRSRVVWL GMRQPPRGLI QLANMLRSQA ARSGCFQSNR 130 140 150 160 170 PFHPHITLLR DASEAVTIPP PGFNWSYAVT EFTLYASSFA RGRTRYTPLK RWALTQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region." Fujita N., Mori H., Yura T., Ishihama A. Nucleic Acids Res. 22:1637-1639(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Nucleotide sequence and characterization of the sfs1 gene: sfs1 is involved in CRP*-dependent mal gene expression in Escherichia coli." Kawamukai M., Utsumi R., Takeda K., Higashi A., Matsuda H., Choi Y.-L., Komano T. J. Bacteriol. 173:2644-2648(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 107-176. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The 2'-5' RNA ligase of Escherichia coli. Purification, cloning, and genomic disruption." Arn E.A., Abelson J.N. J. Biol. Chem. 271:31145-31153(1996) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-23, CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC73258.2. AP009048 Genomic DNA. Translation: BAB96724.2. M60726 Genomic DNA. No translation available.
PIR	C64738.
RefSeq	NP_414689.4. NC_000913.2. YP_488450.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P37025.
SMR	P37025. Positions 5-175.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b0147.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73258; AAC73258; b0147. BAB96724; BAB96724; BAB96724.
GeneID	12930750. 944848.
KEGG	ecj:Y75_p0144. eco:b0147.
PATRIC	32115401. VBIEscCol129921_0152.
Organism-specific databases
EchoBASE	EB2234.
EcoGene	EG12330. ligT.
Phylogenomic databases
eggNOG	COG1514.
HOGENOM	HOG000226389.
KO	K01975.
OMA	DAGHWPR.
ProtClustDB	PRK15124.
Enzyme and pathway databases
BioCyc	EcoCyc:EG12330-MONOMER. ECOL316407:JW5011-MONOMER. MetaCyc:EG12330-MONOMER.
Gene expression databases
Genevestigator	P37025.
Family and domain databases
InterPro	IPR004175. 2_5_ligase. IPR014051. Phosphoesterase_HXTX. IPR009097. RNA_ligase/cNuc_Pdiesterase. [Graphical view]
Pfam	PF02834. LigT_PEase. 2 hits. [Graphical view]
SUPFAM	SSF55144. Cyc_nuc_Pdiester. 1 hit.
TIGRFAMs	TIGR02258. 2_5_ligase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LIGT_ECOLI

Accession

Primary (citable) accession number: P37025
Secondary accession number(s): Q8KJQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 90 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program