Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P37025

- LIGT_ECOLI

UniProt

P37025 - LIGT_ECOLI

Protein

2'-5'-RNA ligase

Gene

ligT

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Specifically ligates tRNA half-molecules containing nucleoside base modifications and shows a preference among different tRNA species. Acts in the absence of ATP to form a 2'-5' phosphodiester linkage.

    GO - Molecular functioni

    1. 2'-5'-RNA ligase activity Source: EcoCyc

    GO - Biological processi

    1. RNA metabolic process Source: InterPro

    Keywords - Molecular functioni

    Ligase

    Enzyme and pathway databases

    BioCyciEcoCyc:EG12330-MONOMER.
    ECOL316407:JW5011-MONOMER.
    MetaCyc:EG12330-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2'-5'-RNA ligase (EC:6.5.1.-)
    Gene namesi
    Name:ligT
    Synonyms:yadP
    Ordered Locus Names:b0147, JW5011
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG12330. ligT.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 1761752'-5'-RNA ligasePRO_0000084421Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriP37025.

    Interactioni

    Protein-protein interaction databases

    STRINGi511145.b0147.

    Structurei

    3D structure databases

    ProteinModelPortaliP37025.
    SMRiP37025. Positions 5-175.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiCOG1514.
    HOGENOMiHOG000226389.
    KOiK01975.
    OMAiDAGHWPR.
    OrthoDBiEOG632D2K.
    PhylomeDBiP37025.

    Family and domain databases

    Gene3Di3.90.1140.10. 1 hit.
    InterProiIPR004175. 2_5_ligase.
    IPR014051. Phosphoesterase_HXTX.
    IPR009097. RNA_ligase/cNuc_Pdiesterase.
    [Graphical view]
    PfamiPF02834. LigT_PEase. 2 hits.
    [Graphical view]
    SUPFAMiSSF55144. SSF55144. 1 hit.
    TIGRFAMsiTIGR02258. 2_5_ligase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P37025-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEPQRLFFA IDLPAEIREQ IIHWRATHFP PEAGRPVAAD NLHLTLAFLG    50
    EVSAEKEKAL SLLAGRIRQP GFTLTLDDAG QWLRSRVVWL GMRQPPRGLI 100
    QLANMLRSQA ARSGCFQSNR PFHPHITLLR DASEAVTIPP PGFNWSYAVT 150
    EFTLYASSFA RGRTRYTPLK RWALTQ 176
    Length:176
    Mass (Da):19,934
    Last modified:January 23, 2007 - v3
    Checksum:i9DAA9FB3CE4F777E
    GO

    Sequence cautioni

    The sequence M60726 differs from that shown. Reason: Frameshift at position 168.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti174 – 1741L → V in M60726. (PubMed:2013578)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC73258.2.
    AP009048 Genomic DNA. Translation: BAB96724.2.
    M60726 Genomic DNA. No translation available.
    PIRiC64738.
    RefSeqiNP_414689.4. NC_000913.3.
    YP_488450.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73258; AAC73258; b0147.
    BAB96724; BAB96724; BAB96724.
    GeneIDi12930750.
    944848.
    KEGGiecj:Y75_p0144.
    eco:b0147.
    PATRICi32115401. VBIEscCol129921_0152.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC73258.2 .
    AP009048 Genomic DNA. Translation: BAB96724.2 .
    M60726 Genomic DNA. No translation available.
    PIRi C64738.
    RefSeqi NP_414689.4. NC_000913.3.
    YP_488450.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P37025.
    SMRi P37025. Positions 5-175.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 511145.b0147.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73258 ; AAC73258 ; b0147 .
    BAB96724 ; BAB96724 ; BAB96724 .
    GeneIDi 12930750.
    944848.
    KEGGi ecj:Y75_p0144.
    eco:b0147.
    PATRICi 32115401. VBIEscCol129921_0152.

    Organism-specific databases

    EchoBASEi EB2234.
    EcoGenei EG12330. ligT.

    Phylogenomic databases

    eggNOGi COG1514.
    HOGENOMi HOG000226389.
    KOi K01975.
    OMAi DAGHWPR.
    OrthoDBi EOG632D2K.
    PhylomeDBi P37025.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG12330-MONOMER.
    ECOL316407:JW5011-MONOMER.
    MetaCyc:EG12330-MONOMER.

    Miscellaneous databases

    PROi P37025.

    Gene expression databases

    Genevestigatori P37025.

    Family and domain databases

    Gene3Di 3.90.1140.10. 1 hit.
    InterProi IPR004175. 2_5_ligase.
    IPR014051. Phosphoesterase_HXTX.
    IPR009097. RNA_ligase/cNuc_Pdiesterase.
    [Graphical view ]
    Pfami PF02834. LigT_PEase. 2 hits.
    [Graphical view ]
    SUPFAMi SSF55144. SSF55144. 1 hit.
    TIGRFAMsi TIGR02258. 2_5_ligase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region."
      Fujita N., Mori H., Yura T., Ishihama A.
      Nucleic Acids Res. 22:1637-1639(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "Nucleotide sequence and characterization of the sfs1 gene: sfs1 is involved in CRP*-dependent mal gene expression in Escherichia coli."
      Kawamukai M., Utsumi R., Takeda K., Higashi A., Matsuda H., Choi Y.-L., Komano T.
      J. Bacteriol. 173:2644-2648(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 107-176.
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "The 2'-5' RNA ligase of Escherichia coli. Purification, cloning, and genomic disruption."
      Arn E.A., Abelson J.N.
      J. Biol. Chem. 271:31145-31153(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-23, CHARACTERIZATION.

    Entry informationi

    Entry nameiLIGT_ECOLI
    AccessioniPrimary (citable) accession number: P37025
    Secondary accession number(s): Q8KJQ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 99 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene

    External Data

    Dasty 3