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P37025 (LIGT_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
2'-5'-RNA ligase

EC=6.5.1.-
Gene names
Name:ligT
Synonyms:yadP
Ordered Locus Names:b0147, JW5011
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length176 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Specifically ligates tRNA half-molecules containing nucleoside base modifications and shows a preference among different tRNA species. Acts in the absence of ATP to form a 2'-5' phosphodiester linkage.

Sequence caution

The sequence M60726 differs from that shown. Reason: Frameshift at position 168.

Ontologies

Keywords
   Molecular functionLigase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processRNA metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_function2'-5'-RNA ligase activity

Inferred from direct assay Ref.5. Source: EcoCyc

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.5
Chain2 – 1761752'-5'-RNA ligase
PRO_0000084421

Experimental info

Sequence conflict1741L → V in M60726. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P37025 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 9DAA9FB3CE4F777E

FASTA17619,934
        10         20         30         40         50         60 
MSEPQRLFFA IDLPAEIREQ IIHWRATHFP PEAGRPVAAD NLHLTLAFLG EVSAEKEKAL 

        70         80         90        100        110        120 
SLLAGRIRQP GFTLTLDDAG QWLRSRVVWL GMRQPPRGLI QLANMLRSQA ARSGCFQSNR 

       130        140        150        160        170 
PFHPHITLLR DASEAVTIPP PGFNWSYAVT EFTLYASSFA RGRTRYTPLK RWALTQ 

« Hide

References

« Hide 'large scale' references
[1]"Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region."
Fujita N., Mori H., Yura T., Ishihama A.
Nucleic Acids Res. 22:1637-1639(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Nucleotide sequence and characterization of the sfs1 gene: sfs1 is involved in CRP*-dependent mal gene expression in Escherichia coli."
Kawamukai M., Utsumi R., Takeda K., Higashi A., Matsuda H., Choi Y.-L., Komano T.
J. Bacteriol. 173:2644-2648(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 107-176.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"The 2'-5' RNA ligase of Escherichia coli. Purification, cloning, and genomic disruption."
Arn E.A., Abelson J.N.
J. Biol. Chem. 271:31145-31153(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-23, CHARACTERIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00096 Genomic DNA. Translation: AAC73258.2.
AP009048 Genomic DNA. Translation: BAB96724.2.
M60726 Genomic DNA. No translation available.
PIRC64738.
RefSeqNP_414689.4. NC_000913.3.
YP_488450.1. NC_007779.1.

3D structure databases

ProteinModelPortalP37025.
SMRP37025. Positions 5-175.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING511145.b0147.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC73258; AAC73258; b0147.
BAB96724; BAB96724; BAB96724.
GeneID12930750.
944848.
KEGGecj:Y75_p0144.
eco:b0147.
PATRIC32115401. VBIEscCol129921_0152.

Organism-specific databases

EchoBASEEB2234.
EcoGeneEG12330. ligT.

Phylogenomic databases

eggNOGCOG1514.
HOGENOMHOG000226389.
KOK01975.
OMADAGHWPR.
OrthoDBEOG632D2K.
PhylomeDBP37025.
ProtClustDBPRK15124.

Enzyme and pathway databases

BioCycEcoCyc:EG12330-MONOMER.
ECOL316407:JW5011-MONOMER.
MetaCyc:EG12330-MONOMER.

Gene expression databases

GenevestigatorP37025.

Family and domain databases

Gene3D3.90.1140.10. 1 hit.
InterProIPR004175. 2_5_ligase.
IPR014051. Phosphoesterase_HXTX.
IPR009097. RNA_ligase/cNuc_Pdiesterase.
[Graphical view]
PfamPF02834. LigT_PEase. 2 hits.
[Graphical view]
SUPFAMSSF55144. SSF55144. 1 hit.
TIGRFAMsTIGR02258. 2_5_ligase. 1 hit.
ProtoNetSearch...

Other

PROP37025.

Entry information

Entry nameLIGT_ECOLI
AccessionPrimary (citable) accession number: P37025
Secondary accession number(s): Q8KJQ1
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 97 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene