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P37020

- GEF1_YEAST

UniProt

P37020 - GEF1_YEAST

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Protein

Anion/proton exchange transporter GEF1

Gene
GEF1, CLCY1, YJR040W, J1616
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Anion/proton exchange transporter involved in iron and copper cation homeostasis. Involved in intracellular iron metabolism during growth on fermentable and non fermentable carbon sources. Required for proper copper-loading and maturation of multicopper oxidase FET3. Important for adjusting intracellular compartment pH to more alkaline pH under iron limitation. May also transport chloride ions through the plasma membrane.5 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei136 – 1372Cleavage; by KEX2
Sitei230 – 2301Mediates proton transfer from the outer aqueous phase to the interior of the protein; involved in linking H(+) and Cl(-) transport By similarity
Sitei287 – 2871Mediates proton transfer from the protein to the inner aqueous phase By similarity

GO - Molecular functioni

  1. adenyl nucleotide binding Source: InterPro
  2. protein binding Source: IntAct
  3. voltage-gated chloride channel activity Source: SGD

GO - Biological processi

  1. cellular copper ion homeostasis Source: SGD
  2. cellular iron ion homeostasis Source: SGD
  3. regulation of anion transport Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Enzyme and pathway databases

BioCyciYEAST:G3O-31675-MONOMER.
ReactomeiREACT_204366. Stimuli-sensing channels.

Protein family/group databases

TCDBi2.A.49.1.1. the chloride carrier/channel (clc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Anion/proton exchange transporter GEF1
Alternative name(s):
CLC protein GEF1
ClC-A
ClC-Y1
Voltage-gated chloride channel
Cleaved into the following 2 chains:
Gene namesi
Name:GEF1
Synonyms:CLCY1
Ordered Locus Names:YJR040W
ORF Names:J1616
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

SGDiS000003801. GEF1.

Subcellular locationi

Vacuole membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein 3 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7575Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei76 – 9621Helical; Reviewed predictionAdd
BLAST
Topological domaini97 – 15458Lumenal Reviewed predictionAdd
BLAST
Transmembranei155 – 17521Helical; Reviewed predictionAdd
BLAST
Topological domaini176 – 1772Cytoplasmic Reviewed prediction
Transmembranei178 – 19821Helical; Reviewed predictionAdd
BLAST
Topological domaini199 – 2035Lumenal Reviewed prediction
Transmembranei204 – 22421Helical; Reviewed predictionAdd
BLAST
Topological domaini225 – 26440Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei265 – 28521Helical; Reviewed predictionAdd
BLAST
Topological domaini286 – 29611Lumenal Reviewed predictionAdd
BLAST
Transmembranei297 – 31923Helical; Reviewed predictionAdd
BLAST
Topological domaini320 – 33617Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei337 – 35721Helical; Reviewed predictionAdd
BLAST
Topological domaini358 – 36912Lumenal Reviewed predictionAdd
BLAST
Transmembranei370 – 39021Helical; Reviewed predictionAdd
BLAST
Topological domaini391 – 43646Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei437 – 45721Helical; Reviewed predictionAdd
BLAST
Topological domaini458 – 4658Lumenal Reviewed prediction
Transmembranei466 – 48621Helical; Reviewed predictionAdd
BLAST
Topological domaini487 – 50014Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei501 – 52323Helical; Reviewed predictionAdd
BLAST
Topological domaini524 – 5296Lumenal Reviewed prediction
Transmembranei530 – 55223Helical; Reviewed predictionAdd
BLAST
Topological domaini553 – 779227Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. chloride channel complex Source: UniProtKB-KW
  2. endoplasmic reticulum Source: SGD
  3. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  4. endosome Source: SGD
  5. endosome membrane Source: UniProtKB-SubCell
  6. fungal-type vacuole Source: SGD
  7. Golgi apparatus Source: SGD
  8. Golgi medial cisterna Source: SGD
  9. Golgi membrane Source: UniProtKB-SubCell
  10. plasma membrane Source: SGD
  11. vacuolar membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 779779Anion/proton exchange transporter GEF1PRO_0000094472Add
BLAST
Chaini1 – 136136GEF1 N-terminalPRO_0000419270Add
BLAST
Chaini137 – 779643GEF1 C-terminalPRO_0000419271Add
BLAST

Post-translational modificationi

Proteolytically processed in the secretory pathway by protease KEX2 within the first extracellular loop. However, both the N- and C-terminal products of the cleavage reaction are required for assembly of a functional channel.

Proteomic databases

MaxQBiP37020.
PaxDbiP37020.

Expressioni

Gene expression databases

GenevestigatoriP37020.

Interactioni

Subunit structurei

Homodimer By similarity. Interacts with GET3.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GET3Q121546EBI-7552,EBI-2989

Protein-protein interaction databases

BioGridi33791. 66 interactions.
DIPiDIP-3949N.
IntActiP37020. 6 interactions.
MINTiMINT-492781.
STRINGi4932.YJR040W.

Structurei

3D structure databases

ProteinModelPortaliP37020.
SMRiP37020. Positions 153-570, 596-735.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini591 – 65969CBS 1Add
BLAST
Domaini688 – 74457CBS 2Add
BLAST

Sequence similaritiesi

Contains 2 CBS domains.

Keywords - Domaini

CBS domain, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0038.
GeneTreeiENSGT00620000087693.
HOGENOMiHOG000164493.
KOiK05012.
OrthoDBiEOG72RN6P.

Family and domain databases

Gene3Di1.10.3080.10. 2 hits.
InterProiIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
[Graphical view]
PfamiPF00571. CBS. 2 hits.
PF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
SUPFAMiSSF81340. SSF81340. 3 hits.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37020-1 [UniParc]FASTAAdd to Basket

« Hide

MPTTYVPINQ PIGDGEDVID TNRFTNIPET QNFDQFVTID KIAEENRPLS    50
VDSDREFLNS KYRHYREVIW DRAKTFITLS STAIVIGCIA GFLQVFTETL 100
VNWKTGHCQR NWLLNKSFCC NGVVNEVTST SNLLLKRQEF ECEAQGLWIA 150
WKGHVSPFII FMLLSVLFAL ISTLLVKYVA PMATGSGISE IKVWVSGFEY 200
NKEFLGFLTL VIKSVALPLA ISSGLSVGKE GPSVHYATCC GYLLTKWLLR 250
DTLTYSSQYE YITAASGAGV AVAFGAPIGG VLFGLEEIAS ANRFNSSTLW 300
KSYYVALVAI TTLKYIDPFR NGRVILFNVT YDRDWKVQEI PIFIALGIFG 350
GLYGKYISKW NINFIHFRKM YLSSWPVQEV LFLATLTALI SYFNEFLKLD 400
MTESMGILFH ECVKNDNTST FSHRLCQLDE NTHAFEFLKI FTSLCFATVI 450
RALLVVVSYG ARVPAGIFVP SMAVGATFGR AVSLLVERFI SGPSVITPGA 500
YAFLGAAATL SGITNLTLTV VVIMFELTGA FMYIIPLMIV VAITRIILST 550
SGISGGIADQ MIMVNGFPYL EDEQDEEEEE TLEKYTAEQL MSSKLITINE 600
TIYLSELESL LYDSASEYSV HGFPITKDED KFEKEKRCIG YVLKRHLASK 650
IMMQSVNSTK AQTTLVYFNK SNEELGHREN CIGFKDIMNE SPISVKKAVP 700
VTLLFRMFKE LGCKTIIVEE SGILKGLVTA KDILRFKRIK YREVHGAKFT 750
YNEALDRRCW SVIHFIIKRF TTNRNGNVI 779
Length:779
Mass (Da):87,592
Last modified:October 5, 2010 - v2
Checksum:i544C751402D32E19
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 131G → R in CAA80663. 1 Publication
Sequence conflicti207 – 2071F → L in CAA80663. 1 Publication
Sequence conflicti257 – 2571S → T in CAA80663. 1 Publication
Sequence conflicti262 – 2621I → L in CAA80663. 1 Publication
Sequence conflicti497 – 4971T → I in CAA80663. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z23117 Genomic DNA. Translation: CAA80663.1.
L29347 Genomic DNA. Translation: AAA53399.1.
Z49540 Genomic DNA. Translation: CAA89567.1.
L36344 Genomic DNA. Translation: AAA88741.1.
BK006943 Genomic DNA. Translation: DAA08827.1.
PIRiS50054.
RefSeqiNP_012574.1. NM_001181698.1.

Genome annotation databases

EnsemblFungiiYJR040W; YJR040W; YJR040W.
GeneIDi853497.
KEGGisce:YJR040W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z23117 Genomic DNA. Translation: CAA80663.1 .
L29347 Genomic DNA. Translation: AAA53399.1 .
Z49540 Genomic DNA. Translation: CAA89567.1 .
L36344 Genomic DNA. Translation: AAA88741.1 .
BK006943 Genomic DNA. Translation: DAA08827.1 .
PIRi S50054.
RefSeqi NP_012574.1. NM_001181698.1.

3D structure databases

ProteinModelPortali P37020.
SMRi P37020. Positions 153-570, 596-735.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33791. 66 interactions.
DIPi DIP-3949N.
IntActi P37020. 6 interactions.
MINTi MINT-492781.
STRINGi 4932.YJR040W.

Protein family/group databases

TCDBi 2.A.49.1.1. the chloride carrier/channel (clc) family.

Proteomic databases

MaxQBi P37020.
PaxDbi P37020.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJR040W ; YJR040W ; YJR040W .
GeneIDi 853497.
KEGGi sce:YJR040W.

Organism-specific databases

SGDi S000003801. GEF1.

Phylogenomic databases

eggNOGi COG0038.
GeneTreei ENSGT00620000087693.
HOGENOMi HOG000164493.
KOi K05012.
OrthoDBi EOG72RN6P.

Enzyme and pathway databases

BioCyci YEAST:G3O-31675-MONOMER.
Reactomei REACT_204366. Stimuli-sensing channels.

Miscellaneous databases

NextBioi 974134.

Gene expression databases

Genevestigatori P37020.

Family and domain databases

Gene3Di 1.10.3080.10. 2 hits.
InterProi IPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
[Graphical view ]
Pfami PF00571. CBS. 2 hits.
PF00654. Voltage_CLC. 1 hit.
[Graphical view ]
PRINTSi PR00762. CLCHANNEL.
SMARTi SM00116. CBS. 2 hits.
[Graphical view ]
SUPFAMi SSF81340. SSF81340. 3 hits.
PROSITEi PS51371. CBS. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The GEF1 gene of Saccharomyces cerevisiae encodes an integral membrane protein; mutations in which have effects on respiration and iron-limited growth."
    Greene J.R., Brown N.H., Didomenico B.J., Kaplan J., Eide D.J.
    Mol. Gen. Genet. 241:542-553(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: ATCC 204508 / S288c.
  2. "A voltage-gated chloride channel in the yeast Saccharomyces cerevisiae."
    Huang M.-E., Chuat J.-C., Galibert F.
    J. Mol. Biol. 242:595-598(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Analysis of a 42.5 kb DNA sequence of chromosome X reveals three tRNA genes and 14 new open reading frames including a gene most probably belonging to the family of ubiquitin-protein ligases."
    Huang M.-E., Chuat J.-C., Galibert F.
    Yeast 11:775-781(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "Golgi localization and functionally important domains in the NH2 and COOH terminus of the yeast CLC putative chloride channel Gef1p."
    Schwappach B., Stobrawa S., Hechenberger M., Steinmeyer K., Jentsch T.J.
    J. Biol. Chem. 273:15110-15118(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. "The yeast CLC chloride channel functions in cation homeostasis."
    Gaxiola R.A., Yuan D.S., Klausner R.D., Fink G.R.
    Proc. Natl. Acad. Sci. U.S.A. 95:4046-4050(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  8. "The Gef1 protein of Saccharomyces cerevisiae is associated with chloride channel activity."
    Flis K., Bednarczyk P., Hordejuk R., Szewczyk A., Berest V., Dolowy K., Edelman A., Kurlandzka A.
    Biochem. Biophys. Res. Commun. 294:1144-1150(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "The yeast CLC chloride channel is proteolytically processed by the furin-like protease Kex2p in the first extracellular loop."
    Wachter A., Schwappach B.
    FEBS Lett. 579:1149-1153(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE BY KEX2, SUBCELLULAR LOCATION.
  10. "The yeast Arr4p ATPase binds the chloride transporter Gef1p when copper is available in the cytosol."
    Metz J., Waechter A., Schmidt B., Bujnicki J.M., Schwappach B.
    J. Biol. Chem. 281:410-417(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GET3.
  11. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  12. "The product of the gene GEF1 of Saccharomyces cerevisiae transports Cl- across the plasma membrane."
    Lopez-Rodriguez A., Trejo A.C., Coyne L., Halliwell R.F., Miledi R., Martinez-Torres A.
    FEMS Yeast Res. 7:1218-1229(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiGEF1_YEAST
AccessioniPrimary (citable) accession number: P37020
Secondary accession number(s): D6VWL1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: October 5, 2010
Last modified: September 3, 2014
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be a voltage-gated ClC-type chloride channel.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

Similar proteinsi