Reviewed,
UniProtKB/Swiss-Prot P37020 (GEF1_YEAST)
Last modified
June 16, 2009.
Version 87.
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Protein GEF1 Alternative name(s): Voltage-gated chloride channel ClC-Y1 ClC-A | ||||||||
| Gene names |
| ||||||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 779 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Transport protein involved in intracellular iron metabolism during growth on fermentable and non fermentable carbon sources. Potential voltage-gated chloride channel. |
| Subunit structure | Homotetramer Probable. Interacts with GET3. |
| Subcellular location | |
| Sequence similarities | Belongs to the chloride channel (TC 2.A.49) family. [View classification] Contains 2 CBS domains. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| AKR1 | P39010 | 1 | EBI-7552,EBI-2421 | |
| GET3 | Q12154 | 5 | EBI-7552,EBI-2989 | |
| KEX1 | P09620 | 1 | EBI-7552,EBI-9653 | |
| PMP2 | P40975 | 1 | EBI-7552,EBI-2043041 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 779 | 779 | Protein GEF1 | PRO_0000094472 | |||||
Regions | |||||||||
| Topological domain | 1 – 75 | 75 | Extracellular Potential | ||||||
| Transmembrane | 76 – 96 | 21 | Potential | ||||||
| Topological domain | 97 – 154 | 58 | Cytoplasmic Potential | ||||||
| Transmembrane | 155 – 175 | 21 | Potential | ||||||
| Topological domain | 176 – 177 | 2 | Extracellular Potential | ||||||
| Transmembrane | 178 – 198 | 21 | Potential | ||||||
| Topological domain | 199 – 203 | 5 | Cytoplasmic Potential | ||||||
| Transmembrane | 204 – 224 | 21 | Potential | ||||||
| Topological domain | 225 – 264 | 40 | Extracellular Potential | ||||||
| Transmembrane | 265 – 285 | 21 | Potential | ||||||
| Topological domain | 286 – 336 | 51 | Cytoplasmic Potential | ||||||
| Transmembrane | 337 – 357 | 21 | Potential | ||||||
| Topological domain | 358 – 436 | 79 | Extracellular Potential | ||||||
| Transmembrane | 437 – 457 | 21 | Potential | ||||||
| Topological domain | 458 – 488 | 31 | Cytoplasmic Potential | ||||||
| Transmembrane | 489 – 509 | 21 | Potential | ||||||
| Topological domain | 510 – 520 | 11 | Extracellular Potential | ||||||
| Transmembrane | 521 – 541 | 21 | Potential | ||||||
| Topological domain | 542 – 779 | 238 | Cytoplasmic Potential | ||||||
| Domain | 591 – 659 | 69 | CBS 1 | ||||||
| Domain | 688 – 744 | 57 | CBS 2 | ||||||
Amino acid modifications | |||||||||
| Modified residue | 214 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 226 | 1 | Phosphoserine Ref.7 | ||||||
Experimental info | |||||||||
| Sequence conflict | 13 | 1 | R → G in AAA53399. Ref.2 | ||||||
| Sequence conflict | 13 | 1 | R → G in CAA89567. Ref.2 | ||||||
| Sequence conflict | 13 | 1 | R → G in AAA88741. Ref.2 | ||||||
| Sequence conflict | 207 | 1 | L → F in AAA53399. Ref.2 | ||||||
| Sequence conflict | 207 | 1 | L → F in CAA89567. Ref.2 | ||||||
| Sequence conflict | 207 | 1 | L → F in AAA88741. Ref.2 | ||||||
| Sequence conflict | 257 | 1 | T → S in AAA53399. Ref.2 | ||||||
| Sequence conflict | 257 | 1 | T → S in CAA89567. Ref.2 | ||||||
| Sequence conflict | 257 | 1 | T → S in AAA88741. Ref.2 | ||||||
| Sequence conflict | 262 | 1 | L → I in AAA53399. Ref.2 | ||||||
| Sequence conflict | 262 | 1 | L → I in CAA89567. Ref.2 | ||||||
| Sequence conflict | 262 | 1 | L → I in AAA88741. Ref.2 | ||||||
| Sequence conflict | 497 | 1 | I → T in AAA53399. Ref.2 | ||||||
| Sequence conflict | 497 | 1 | I → T in CAA89567. Ref.2 | ||||||
| Sequence conflict | 497 | 1 | I → T in AAA88741. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The GEF1 gene of Saccharomyces cerevisiae encodes an integral membrane protein; mutations in which have effects on respiration and iron-limited growth." Greene J.R., Brown N.H., Didomenico B.J., Kaplan J., Eide D.J. Mol. Gen. Genet. 241:542-553(1993) [PubMed: 7505388] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [2] | "A voltage-gated chloride channel in the yeast Saccharomyces cerevisiae." Huang M.-E., Chuat J.-C., Galibert F. J. Mol. Biol. 242:595-598(1994) [PubMed: 7932715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [3] | "Analysis of a 42.5 kb DNA sequence of chromosome X reveals three tRNA genes and 14 new open reading frames including a gene most probably belonging to the family of ubiquitin-protein ligases." Huang M.-E., Chuat J.-C., Galibert F. Yeast 11:775-781(1995) [PubMed: 7668047] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [4] | "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L.EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [5] | "The yeast Arr4p ATPase binds the chloride transporter Gef1p when copper is available in the cytosol." Metz J., Waechter A., Schmidt B., Bujnicki J.M., Schwappach B. J. Biol. Chem. 281:410-417(2006) [PubMed: 16260785] [Abstract] Cited for: INTERACTION WITH GET3. |
| [6] | "A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. |
| [7] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-214 AND SER-226, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| Z23117 Genomic DNA. Translation: CAA80663.1. L29347 Genomic DNA. Translation: AAA53399.1. Z49540 Genomic DNA. Translation: CAA89567.1. L36344 Genomic DNA. Translation: AAA88741.1. | |
| PIR | S50054. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:3949N. |
| IntAct | P37020. 7 interactions. |
Protein family/group databases | |
| TCDB | 2.A.49.1.1. chloride carrier/channel (ClC) family. |
Genome annotation databases | |
| Ensembl | YJR040W. Saccharomyces cerevisiae. [Contig view] |
| GenomeReviews | Gene locus YJR040W in contig Y13136_GR. |
| KEGG | sce:YJR040W. |
Organism-specific databases | |
| CYGD | YJR040w. |
| SGD | S000003801. GEF1. |
| Yeast-GFP | Search... |
Phylogenomic databases | |
| HOGENOM | P37020. |
Gene expression databases | |
| ArrayExpress | P37020. |
| GermOnline | YJR040W. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR014743. Cl-channel_core. IPR001807. Cl-channel_volt. IPR000644. Cysta_beta_synth_core. [Graphical view] |
| Gene3D | G3DSA:1.10.3080.10. Cl-channel_core. 1 hit. |
| PANTHER | PTHR11689. Cl-channel_volt. 1 hit. |
| Pfam | PF00571. CBS. 1 hit. PF00654. Voltage_CLC. 1 hit. [Graphical view] |
| PRINTS | PR00762. CLCHANNEL. |
| SMART | SM00116. CBS. 2 hits. [Graphical view] |
| PROSITE | PS51371. CBS. 2 hits. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | GEF1_YEAST | ||||||||
| Accession | Primary (citable) accession number: P37020 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome X Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names |

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