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P37020 (GEF1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 136. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Anion/proton exchange transporter GEF1
Alternative name(s):
CLC protein GEF1
ClC-A
ClC-Y1
Voltage-gated chloride channel

Cleaved into the following 2 chains:

  1. GEF1 N-terminal
  2. GEF1 C-terminal
Gene names
Name:GEF1
Synonyms:CLCY1
Ordered Locus Names:YJR040W
ORF Names:J1616
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length779 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Anion/proton exchange transporter involved in iron and copper cation homeostasis. Involved in intracellular iron metabolism during growth on fermentable and non fermentable carbon sources. Required for proper copper-loading and maturation of multicopper oxidase FET3. Important for adjusting intracellular compartment pH to more alkaline pH under iron limitation. May also transport chloride ions through the plasma membrane. Ref.1 Ref.6 Ref.7 Ref.8 Ref.12

Subunit structure

Homodimer By similarity. Interacts with GET3. Ref.10

Subcellular location

Vacuole membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein Ref.6 Ref.7 Ref.9.

Post-translational modification

Proteolytically processed in the secretory pathway by protease KEX2 within the first extracellular loop. However, both the N- and C-terminal products of the cleavage reaction are required for assembly of a functional channel.

Sequence similarities

Belongs to the chloride channel (TC 2.A.49) family. [View classification]

Contains 2 CBS domains.

Caution

Was originally thought to be a voltage-gated ClC-type chloride channel.

Ontologies

Keywords
   Biological processIon transport
Transport
   Cellular componentCell membrane
Endoplasmic reticulum
Endosome
Golgi apparatus
Membrane
Vacuole
   DomainCBS domain
Repeat
Transmembrane
Transmembrane helix
   LigandChloride
   Molecular functionChloride channel
Ion channel
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular copper ion homeostasis

Inferred from mutant phenotype Ref.7Ref.6. Source: SGD

cellular iron ion homeostasis

Inferred from mutant phenotype Ref.7. Source: SGD

regulation of anion transport

Inferred from direct assay Ref.8. Source: GOC

   Cellular_componentGolgi apparatus

Inferred from direct assay Ref.9. Source: SGD

Golgi medial cisterna

Inferred from direct assay Ref.6. Source: SGD

Golgi membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

chloride channel complex

Inferred from electronic annotation. Source: UniProtKB-KW

endoplasmic reticulum

Inferred from direct assay Ref.12. Source: SGD

endoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

endosome

Inferred from direct assay Ref.9. Source: SGD

endosome membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

fungal-type vacuole

Inferred from direct assay Ref.12. Source: SGD

plasma membrane

Inferred from direct assay Ref.12. Source: SGD

vacuolar membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionadenyl nucleotide binding

Inferred from electronic annotation. Source: InterPro

voltage-gated chloride channel activity

Inferred from direct assay Ref.8. Source: SGD

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

GET3Q121546EBI-7552,EBI-2989

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 779779Anion/proton exchange transporter GEF1
PRO_0000094472
Chain1 – 136136GEF1 N-terminal
PRO_0000419270
Chain137 – 779643GEF1 C-terminal
PRO_0000419271

Regions

Topological domain1 – 7575Cytoplasmic Potential
Transmembrane76 – 9621Helical; Potential
Topological domain97 – 15458Lumenal Potential
Transmembrane155 – 17521Helical; Potential
Topological domain176 – 1772Cytoplasmic Potential
Transmembrane178 – 19821Helical; Potential
Topological domain199 – 2035Lumenal Potential
Transmembrane204 – 22421Helical; Potential
Topological domain225 – 26440Cytoplasmic Potential
Transmembrane265 – 28521Helical; Potential
Topological domain286 – 29611Lumenal Potential
Transmembrane297 – 31923Helical; Potential
Topological domain320 – 33617Cytoplasmic Potential
Transmembrane337 – 35721Helical; Potential
Topological domain358 – 36912Lumenal Potential
Transmembrane370 – 39021Helical; Potential
Topological domain391 – 43646Cytoplasmic Potential
Transmembrane437 – 45721Helical; Potential
Topological domain458 – 4658Lumenal Potential
Transmembrane466 – 48621Helical; Potential
Topological domain487 – 50014Cytoplasmic Potential
Transmembrane501 – 52323Helical; Potential
Topological domain524 – 5296Lumenal Potential
Transmembrane530 – 55223Helical; Potential
Topological domain553 – 779227Cytoplasmic Potential
Domain591 – 65969CBS 1
Domain688 – 74457CBS 2

Sites

Site136 – 1372Cleavage; by KEX2
Site2301Mediates proton transfer from the outer aqueous phase to the interior of the protein; involved in linking H(+) and Cl(-) transport By similarity
Site2871Mediates proton transfer from the protein to the inner aqueous phase By similarity

Experimental info

Sequence conflict131G → R in CAA80663. Ref.1
Sequence conflict2071F → L in CAA80663. Ref.1
Sequence conflict2571S → T in CAA80663. Ref.1
Sequence conflict2621I → L in CAA80663. Ref.1
Sequence conflict4971T → I in CAA80663. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P37020 [UniParc].

Last modified October 5, 2010. Version 2.
Checksum: 544C751402D32E19

FASTA77987,592
        10         20         30         40         50         60 
MPTTYVPINQ PIGDGEDVID TNRFTNIPET QNFDQFVTID KIAEENRPLS VDSDREFLNS 

        70         80         90        100        110        120 
KYRHYREVIW DRAKTFITLS STAIVIGCIA GFLQVFTETL VNWKTGHCQR NWLLNKSFCC 

       130        140        150        160        170        180 
NGVVNEVTST SNLLLKRQEF ECEAQGLWIA WKGHVSPFII FMLLSVLFAL ISTLLVKYVA 

       190        200        210        220        230        240 
PMATGSGISE IKVWVSGFEY NKEFLGFLTL VIKSVALPLA ISSGLSVGKE GPSVHYATCC 

       250        260        270        280        290        300 
GYLLTKWLLR DTLTYSSQYE YITAASGAGV AVAFGAPIGG VLFGLEEIAS ANRFNSSTLW 

       310        320        330        340        350        360 
KSYYVALVAI TTLKYIDPFR NGRVILFNVT YDRDWKVQEI PIFIALGIFG GLYGKYISKW 

       370        380        390        400        410        420 
NINFIHFRKM YLSSWPVQEV LFLATLTALI SYFNEFLKLD MTESMGILFH ECVKNDNTST 

       430        440        450        460        470        480 
FSHRLCQLDE NTHAFEFLKI FTSLCFATVI RALLVVVSYG ARVPAGIFVP SMAVGATFGR 

       490        500        510        520        530        540 
AVSLLVERFI SGPSVITPGA YAFLGAAATL SGITNLTLTV VVIMFELTGA FMYIIPLMIV 

       550        560        570        580        590        600 
VAITRIILST SGISGGIADQ MIMVNGFPYL EDEQDEEEEE TLEKYTAEQL MSSKLITINE 

       610        620        630        640        650        660 
TIYLSELESL LYDSASEYSV HGFPITKDED KFEKEKRCIG YVLKRHLASK IMMQSVNSTK 

       670        680        690        700        710        720 
AQTTLVYFNK SNEELGHREN CIGFKDIMNE SPISVKKAVP VTLLFRMFKE LGCKTIIVEE 

       730        740        750        760        770 
SGILKGLVTA KDILRFKRIK YREVHGAKFT YNEALDRRCW SVIHFIIKRF TTNRNGNVI 

« Hide

References

« Hide 'large scale' references
[1]"The GEF1 gene of Saccharomyces cerevisiae encodes an integral membrane protein; mutations in which have effects on respiration and iron-limited growth."
Greene J.R., Brown N.H., Didomenico B.J., Kaplan J., Eide D.J.
Mol. Gen. Genet. 241:542-553(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
Strain: ATCC 204508 / S288c.
[2]"A voltage-gated chloride channel in the yeast Saccharomyces cerevisiae."
Huang M.-E., Chuat J.-C., Galibert F.
J. Mol. Biol. 242:595-598(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Analysis of a 42.5 kb DNA sequence of chromosome X reveals three tRNA genes and 14 new open reading frames including a gene most probably belonging to the family of ubiquitin-protein ligases."
Huang M.-E., Chuat J.-C., Galibert F.
Yeast 11:775-781(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Golgi localization and functionally important domains in the NH2 and COOH terminus of the yeast CLC putative chloride channel Gef1p."
Schwappach B., Stobrawa S., Hechenberger M., Steinmeyer K., Jentsch T.J.
J. Biol. Chem. 273:15110-15118(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]"The yeast CLC chloride channel functions in cation homeostasis."
Gaxiola R.A., Yuan D.S., Klausner R.D., Fink G.R.
Proc. Natl. Acad. Sci. U.S.A. 95:4046-4050(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]"The Gef1 protein of Saccharomyces cerevisiae is associated with chloride channel activity."
Flis K., Bednarczyk P., Hordejuk R., Szewczyk A., Berest V., Dolowy K., Edelman A., Kurlandzka A.
Biochem. Biophys. Res. Commun. 294:1144-1150(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"The yeast CLC chloride channel is proteolytically processed by the furin-like protease Kex2p in the first extracellular loop."
Wachter A., Schwappach B.
FEBS Lett. 579:1149-1153(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: CLEAVAGE BY KEX2, SUBCELLULAR LOCATION.
[10]"The yeast Arr4p ATPase binds the chloride transporter Gef1p when copper is available in the cytosol."
Metz J., Waechter A., Schmidt B., Bujnicki J.M., Schwappach B.
J. Biol. Chem. 281:410-417(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GET3.
[11]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[12]"The product of the gene GEF1 of Saccharomyces cerevisiae transports Cl- across the plasma membrane."
Lopez-Rodriguez A., Trejo A.C., Coyne L., Halliwell R.F., Miledi R., Martinez-Torres A.
FEMS Yeast Res. 7:1218-1229(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z23117 Genomic DNA. Translation: CAA80663.1.
L29347 Genomic DNA. Translation: AAA53399.1.
Z49540 Genomic DNA. Translation: CAA89567.1.
L36344 Genomic DNA. Translation: AAA88741.1.
BK006943 Genomic DNA. Translation: DAA08827.1.
PIRS50054.
RefSeqNP_012574.1. NM_001181698.1.

3D structure databases

ProteinModelPortalP37020.
SMRP37020. Positions 69-103, 153-570, 596-736.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33791. 65 interactions.
DIPDIP-3949N.
IntActP37020. 6 interactions.
MINTMINT-492781.
STRING4932.YJR040W.

Protein family/group databases

TCDB2.A.49.1.1. the chloride carrier/channel (clc) family.

Proteomic databases

PaxDbP37020.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJR040W; YJR040W; YJR040W.
GeneID853497.
KEGGsce:YJR040W.

Organism-specific databases

SGDS000003801. GEF1.

Phylogenomic databases

eggNOGCOG0038.
GeneTreeENSGT00620000087693.
HOGENOMHOG000164493.
KOK05012.
OMADENTHAF.
OrthoDBEOG72RN6P.

Enzyme and pathway databases

BioCycYEAST:G3O-31675-MONOMER.

Gene expression databases

GenevestigatorP37020.

Family and domain databases

Gene3D1.10.3080.10. 2 hits.
InterProIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
[Graphical view]
PfamPF00571. CBS. 2 hits.
PF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSPR00762. CLCHANNEL.
SMARTSM00116. CBS. 2 hits.
[Graphical view]
SUPFAMSSF81340. SSF81340. 3 hits.
PROSITEPS51371. CBS. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio974134.

Entry information

Entry nameGEF1_YEAST
AccessionPrimary (citable) accession number: P37020
Secondary accession number(s): D6VWL1
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: October 5, 2010
Last modified: April 16, 2014
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families