Anion/proton exchange transporter GEF1 precursor - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P37020 (GEF1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 127. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Anion/proton exchange transporter GEF1

Alternative name(s):
CLC protein GEF1
ClC-A
ClC-Y1
Voltage-gated chloride channel

Gene names

Name:	GEF1
Synonyms:	CLCY1
Ordered Locus Names:	YJR040W
ORF Names:	J1616

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	779 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Anion/proton exchange transporter involved in iron and copper cation homeostasis. Involved in intracellular iron metabolism during growth on fermentable and non fermentable carbon sources. Required for proper copper-loading and maturation of multicopper oxidase FET3. Important for adjusting intracellular compartment pH to more alkaline pH under iron limitation. May also transport chloride ions through the plasma membrane. Ref.1 Ref.6 Ref.7 Ref.8 Ref.12
Subunit structure	Homodimer By similarity. Interacts with GET3. Ref.10
Subcellular location	Vacuole membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein Ref.6 Ref.7 Ref.9.
Post-translational modification	Proteolytically processed in the secretory pathway by protease KEX2 within the first extracellular loop. However, both the N- and C-terminal products of the cleavage reaction are required for assembly of a functional channel.
Sequence similarities	Belongs to the chloride channel (TC 2.A.49) family. [View classification] Contains 2 CBS domains.
Caution	Was originally thought to be a voltage-gated ClC-type chloride channel.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell membrane Endoplasmic reticulum Endosome Golgi apparatus Membrane Vacuole
Domain	CBS domain Repeat Transmembrane Transmembrane helix
Ligand	Chloride
Molecular function	Chloride channel Ion channel
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular copper ion homeostasis Inferred from mutant phenotype Ref.7 Ref.6. Source: SGD cellular iron ion homeostasis Inferred from mutant phenotype Ref.7. Source: SGD
Cellular_component	Golgi medial cisterna Inferred from direct assay Ref.6. Source: SGD Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell chloride channel complex Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum Inferred from direct assay Ref.12. Source: SGD endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endosome Inferred from direct assay Ref.9. Source: SGD endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell fungal-type vacuole Inferred from direct assay Ref.12. Source: SGD integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD plasma membrane Inferred from direct assay Ref.12. Source: SGD vacuolar membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	voltage-gated chloride channel activity Inferred from direct assay Ref.8. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GET3	Q12154	6	EBI-7552,EBI-2989

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 779

779

Anion/proton exchange transporter GEF1

PRO_0000094472

Chain

1 – 136

136

GEF1 N-terminal

PRO_0000419270

Chain

137 – 779

643

GEF1 C-terminal

PRO_0000419271

Regions

Topological domain

1 – 75

Cytoplasmic Potential

Transmembrane

76 – 96

Helical; Potential

Topological domain

97 – 154

Lumenal Potential

Transmembrane

155 – 175

Helical; Potential

Topological domain

176 – 177

Cytoplasmic Potential

Transmembrane

178 – 198

Helical; Potential

Topological domain

199 – 203

Lumenal Potential

Transmembrane

204 – 224

Helical; Potential

Topological domain

225 – 264

Cytoplasmic Potential

Transmembrane

265 – 285

Helical; Potential

Topological domain

286 – 296

Lumenal Potential

Transmembrane

297 – 319

Helical; Potential

Topological domain

320 – 336

Cytoplasmic Potential

Transmembrane

337 – 357

Helical; Potential

Topological domain

358 – 369

Lumenal Potential

Transmembrane

370 – 390

Helical; Potential

Topological domain

391 – 436

Cytoplasmic Potential

Transmembrane

437 – 457

Helical; Potential

Topological domain

458 – 465

Lumenal Potential

Transmembrane

466 – 486

Helical; Potential

Topological domain

487 – 500

Cytoplasmic Potential

Transmembrane

501 – 523

Helical; Potential

Topological domain

524 – 529

Lumenal Potential

Transmembrane

530 – 552

Helical; Potential

Topological domain

553 – 779

227

Cytoplasmic Potential

Domain

591 – 659

CBS 1

Domain

688 – 744

CBS 2

Sites

Site

136 – 137

Cleavage; by KEX2

Site

230

Mediates proton transfer from the outer aqueous phase to the interior of the protein; involved in linking H(+) and Cl(-) transport By similarity

Site

287

Mediates proton transfer from the protein to the inner aqueous phase By similarity

Amino acid modifications

Modified residue

214

Phosphoserine Ref.13

Modified residue

226

Phosphoserine Ref.13

Experimental info

Sequence conflict

G → R in CAA80663. Ref.1

Sequence conflict

207

F → L in CAA80663. Ref.1

Sequence conflict

257

S → T in CAA80663. Ref.1

Sequence conflict

262

I → L in CAA80663. Ref.1

Sequence conflict

497

T → I in CAA80663. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P37020 [UniParc].

Last modified October 5, 2010. Version 2.
Checksum: 544C751402D32E19
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FASTA

779

87,592

        10         20         30         40         50         60 
MPTTYVPINQ PIGDGEDVID TNRFTNIPET QNFDQFVTID KIAEENRPLS VDSDREFLNS 

        70         80         90        100        110        120 
KYRHYREVIW DRAKTFITLS STAIVIGCIA GFLQVFTETL VNWKTGHCQR NWLLNKSFCC 

       130        140        150        160        170        180 
NGVVNEVTST SNLLLKRQEF ECEAQGLWIA WKGHVSPFII FMLLSVLFAL ISTLLVKYVA 

       190        200        210        220        230        240 
PMATGSGISE IKVWVSGFEY NKEFLGFLTL VIKSVALPLA ISSGLSVGKE GPSVHYATCC 

       250        260        270        280        290        300 
GYLLTKWLLR DTLTYSSQYE YITAASGAGV AVAFGAPIGG VLFGLEEIAS ANRFNSSTLW 

       310        320        330        340        350        360 
KSYYVALVAI TTLKYIDPFR NGRVILFNVT YDRDWKVQEI PIFIALGIFG GLYGKYISKW 

       370        380        390        400        410        420 
NINFIHFRKM YLSSWPVQEV LFLATLTALI SYFNEFLKLD MTESMGILFH ECVKNDNTST 

       430        440        450        460        470        480 
FSHRLCQLDE NTHAFEFLKI FTSLCFATVI RALLVVVSYG ARVPAGIFVP SMAVGATFGR 

       490        500        510        520        530        540 
AVSLLVERFI SGPSVITPGA YAFLGAAATL SGITNLTLTV VVIMFELTGA FMYIIPLMIV 

       550        560        570        580        590        600 
VAITRIILST SGISGGIADQ MIMVNGFPYL EDEQDEEEEE TLEKYTAEQL MSSKLITINE 

       610        620        630        640        650        660 
TIYLSELESL LYDSASEYSV HGFPITKDED KFEKEKRCIG YVLKRHLASK IMMQSVNSTK 

       670        680        690        700        710        720 
AQTTLVYFNK SNEELGHREN CIGFKDIMNE SPISVKKAVP VTLLFRMFKE LGCKTIIVEE 

       730        740        750        760        770 
SGILKGLVTA KDILRFKRIK YREVHGAKFT YNEALDRRCW SVIHFIIKRF TTNRNGNVI

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The GEF1 gene of Saccharomyces cerevisiae encodes an integral membrane protein; mutations in which have effects on respiration and iron-limited growth." Greene J.R., Brown N.H., Didomenico B.J., Kaplan J., Eide D.J. Mol. Gen. Genet. 241:542-553(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: ATCC 204508 / S288c.
[2]	"A voltage-gated chloride channel in the yeast Saccharomyces cerevisiae." Huang M.-E., Chuat J.-C., Galibert F. J. Mol. Biol. 242:595-598(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Analysis of a 42.5 kb DNA sequence of chromosome X reveals three tRNA genes and 14 new open reading frames including a gene most probably belonging to the family of ubiquitin-protein ligases." Huang M.-E., Chuat J.-C., Galibert F. Yeast 11:775-781(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Golgi localization and functionally important domains in the NH2 and COOH terminus of the yeast CLC putative chloride channel Gef1p." Schwappach B., Stobrawa S., Hechenberger M., Steinmeyer K., Jentsch T.J. J. Biol. Chem. 273:15110-15118(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]	"The yeast CLC chloride channel functions in cation homeostasis." Gaxiola R.A., Yuan D.S., Klausner R.D., Fink G.R. Proc. Natl. Acad. Sci. U.S.A. 95:4046-4050(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]	"The Gef1 protein of Saccharomyces cerevisiae is associated with chloride channel activity." Flis K., Bednarczyk P., Hordejuk R., Szewczyk A., Berest V., Dolowy K., Edelman A., Kurlandzka A. Biochem. Biophys. Res. Commun. 294:1144-1150(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"The yeast CLC chloride channel is proteolytically processed by the furin-like protease Kex2p in the first extracellular loop." Wachter A., Schwappach B. FEBS Lett. 579:1149-1153(2005) [PubMed] [Europe PMC] [Abstract] Cited for: CLEAVAGE BY KEX2, SUBCELLULAR LOCATION.
[10]	"The yeast Arr4p ATPase binds the chloride transporter Gef1p when copper is available in the cytosol." Metz J., Waechter A., Schmidt B., Bujnicki J.M., Schwappach B. J. Biol. Chem. 281:410-417(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GET3.
[11]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[12]	"The product of the gene GEF1 of Saccharomyces cerevisiae transports Cl- across the plasma membrane." Lopez-Rodriguez A., Trejo A.C., Coyne L., Halliwell R.F., Miledi R., Martinez-Torres A. FEMS Yeast Res. 7:1218-1229(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[13]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-214 AND SER-226, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z23117 Genomic DNA. Translation: CAA80663.1. L29347 Genomic DNA. Translation: AAA53399.1. Z49540 Genomic DNA. Translation: CAA89567.1. L36344 Genomic DNA. Translation: AAA88741.1. BK006943 Genomic DNA. Translation: DAA08827.1.
PIR	S50054.
RefSeq	NP_012574.1. NM_001181698.1.
3D structure databases
ProteinModelPortal	P37020.
SMR	P37020. Positions 153-570, 596-735.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3949N.
IntAct	P37020. 4 interactions.
MINT	MINT-492781.
STRING	4932.YJR040W.
Protein family/group databases
TCDB	2.A.49.1.1. chloride carrier/channel (ClC) family.
Proteomic databases
PaxDb	P37020.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJR040W; YJR040W; YJR040W.
GeneID	853497.
KEGG	sce:YJR040W.
Organism-specific databases
SGD	S000003801. GEF1.
Phylogenomic databases
eggNOG	COG0038.
GeneTree	ENSGT00620000087693.
HOGENOM	HOG000164493.
KO	K05393.
OMA	GILFHEC.
OrthoDB	EOG48WG9C.
Gene expression databases
Genevestigator	P37020.
GermOnline	YJR040W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.3080.10. 2 hits.
InterPro	IPR014743. Cl-channel_core. IPR001807. Cl-channel_volt-gated. IPR000644. Cysta_beta_synth_core. [Graphical view]
Pfam	PF00571. CBS. 2 hits. PF00654. Voltage_CLC. 1 hit. [Graphical view]
PRINTS	PR00762. CLCHANNEL.
SMART	SM00116. CBS. 2 hits. [Graphical view]
SUPFAM	SSF81340. Cl-channel_core. 1 hit.
PROSITE	PS51371. CBS. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	974134.

Entry information

Entry name

GEF1_YEAST

Accession

Primary (citable) accession number: P37020
Secondary accession number(s): D6VWL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	October 5, 2010
Last modified:	May 1, 2013
This is version 127 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Cleaved into the following 2 chains:

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents