Protein GEF1 - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P37020 (GEF1_YEAST)

Last modified June 16, 2009. Version 87. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Protein GEF1
Alternative name(s):
    Voltage-gated chloride channel
    ClC-Y1
    ClC-A

Gene names

Name:	GEF1
Synonyms:	CLCY1
Ordered Locus Names:	YJR040W
ORF Names:	J1616

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	779 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Transport protein involved in intracellular iron metabolism during growth on fermentable and non fermentable carbon sources. Potential voltage-gated chloride channel.
Subunit structure	Homotetramer Probable. Interacts with GET3.
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the chloride channel (TC 2.A.49) family. [View classification] Contains 2 CBS domains.

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Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Membrane
Domain	CBS domain Repeat Transmembrane
Ligand	Chloride
Molecular function	Chloride channel Ionic channel Voltage-gated channel
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular copper ion homeostasis Inferred from mutant phenotype. Source: SGD cellular iron ion homeostasis Inferred from mutant phenotype. Source: SGD chloride transport Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi medial cisterna Inferred from direct assay. Source: SGD endoplasmic reticulum Inferred from direct assay. Source: SGD endosome Inferred from direct assay. Source: SGD fungal-type vacuole Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay. Source: SGD
Molecular function	chloride ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Ref.5 Inferred from physical interaction. Source: IntAct voltage-gated chloride channel activity Inferred from direct assay. Source: SGD
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
AKR1	P39010	1	EBI-7552,EBI-2421
GET3	Q12154	5	EBI-7552,EBI-2989
KEX1	P09620	1	EBI-7552,EBI-9653
PMP2	P40975	1	EBI-7552,EBI-2043041

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 779

779

Protein GEF1

PRO_0000094472

Regions

Topological domain

1 – 75

Extracellular Potential

Transmembrane

76 – 96

Potential

Topological domain

97 – 154

Cytoplasmic Potential

Transmembrane

155 – 175

Potential

Topological domain

176 – 177

Extracellular Potential

Transmembrane

178 – 198

Potential

Topological domain

199 – 203

Cytoplasmic Potential

Transmembrane

204 – 224

Potential

Topological domain

225 – 264

Extracellular Potential

Transmembrane

265 – 285

Potential

Topological domain

286 – 336

Cytoplasmic Potential

Transmembrane

337 – 357

Potential

Topological domain

358 – 436

Extracellular Potential

Transmembrane

437 – 457

Potential

Topological domain

458 – 488

Cytoplasmic Potential

Transmembrane

489 – 509

Potential

Topological domain

510 – 520

Extracellular Potential

Transmembrane

521 – 541

Potential

Topological domain

542 – 779

238

Cytoplasmic Potential

Domain

591 – 659

CBS 1

Domain

688 – 744

CBS 2

Amino acid modifications

Modified residue

214

Phosphoserine Ref.7

Modified residue

226

Phosphoserine Ref.7

Experimental info

Sequence conflict

R → G in AAA53399. Ref.2

Sequence conflict

R → G in CAA89567. Ref.2

Sequence conflict

R → G in AAA88741. Ref.2

Sequence conflict

207

L → F in AAA53399. Ref.2

Sequence conflict

207

L → F in CAA89567. Ref.2

Sequence conflict

207

L → F in AAA88741. Ref.2

Sequence conflict

257

T → S in AAA53399. Ref.2

Sequence conflict

257

T → S in CAA89567. Ref.2

Sequence conflict

257

T → S in AAA88741. Ref.2

Sequence conflict

262

L → I in AAA53399. Ref.2

Sequence conflict

262

L → I in CAA89567. Ref.2

Sequence conflict

262

L → I in AAA88741. Ref.2

Sequence conflict

497

I → T in AAA53399. Ref.2

Sequence conflict

497

I → T in CAA89567. Ref.2

Sequence conflict

497

I → T in AAA88741. Ref.2

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Sequences

Sequence

Length

Mass (Da)

Tools

P37020-1 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 56D86BB3DF2FB25C
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FASTA

779

87,683

        10         20         30         40         50         60 
MPTTYVPINQ PIRDGEDVID TNRFTNIPET QNFDQFVTID KIAEENRPLS VDSDREFLNS 

        70         80         90        100        110        120 
KYRHYREVIW DRAKTFITLS STAIVIGCIA GFLQVFTETL VNWKTGHCQR NWLLNKSFCC 

       130        140        150        160        170        180 
NGVVNEVTST SNLLLKRQEF ECEAQGLWIA WKGHVSPFII FMLLSVLFAL ISTLLVKYVA 

       190        200        210        220        230        240 
PMATGSGISE IKVWVSGFEY NKEFLGLLTL VIKSVALPLA ISSGLSVGKE GPSVHYATCC 

       250        260        270        280        290        300 
GYLLTKWLLR DTLTYSTQYE YLTAASGAGV AVAFGAPIGG VLFGLEEIAS ANRFNSSTLW 

       310        320        330        340        350        360 
KSYYVALVAI TTLKYIDPFR NGRVILFNVT YDRDWKVQEI PIFIALGIFG GLYGKYISKW 

       370        380        390        400        410        420 
NINFIHFRKM YLSSWPVQEV LFLATLTALI SYFNEFLKLD MTESMGILFH ECVKNDNTST 

       430        440        450        460        470        480 
FSHRLCQLDE NTHAFEFLKI FTSLCFATVI RALLVVVSYG ARVPAGIFVP SMAVGATFGR 

       490        500        510        520        530        540 
AVSLLVERFI SGPSVIIPGA YAFLGAAATL SGITNLTLTV VVIMFELTGA FMYIIPLMIV 

       550        560        570        580        590        600 
VAITRIILST SGISGGIADQ MIMVNGFPYL EDEQDEEEEE TLEKYTAEQL MSSKLITINE 

       610        620        630        640        650        660 
TIYLSELESL LYDSASEYSV HGFPITKDED KFEKEKRCIG YVLKRHLASK IMMQSVNSTK 

       670        680        690        700        710        720 
AQTTLVYFNK SNEELGHREN CIGFKDIMNE SPISVKKAVP VTLLFRMFKE LGCKTIIVEE 

       730        740        750        760        770 
SGILKGLVTA KDILRFKRIK YREVHGAKFT YNEALDRRCW SVIHFIIKRF TTNRNGNVI

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The GEF1 gene of Saccharomyces cerevisiae encodes an integral membrane protein; mutations in which have effects on respiration and iron-limited growth." Greene J.R., Brown N.H., Didomenico B.J., Kaplan J., Eide D.J. Mol. Gen. Genet. 241:542-553(1993) [PubMed: 7505388] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"A voltage-gated chloride channel in the yeast Saccharomyces cerevisiae." Huang M.-E., Chuat J.-C., Galibert F. J. Mol. Biol. 242:595-598(1994) [PubMed: 7932715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Analysis of a 42.5 kb DNA sequence of chromosome X reveals three tRNA genes and 14 new open reading frames including a gene most probably belonging to the family of ubiquitin-protein ligases." Huang M.-E., Chuat J.-C., Galibert F. Yeast 11:775-781(1995) [PubMed: 7668047] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"The yeast Arr4p ATPase binds the chloride transporter Gef1p when copper is available in the cytosol." Metz J., Waechter A., Schmidt B., Bujnicki J.M., Schwappach B. J. Biol. Chem. 281:410-417(2006) [PubMed: 16260785] [Abstract] Cited for: INTERACTION WITH GET3.
[6]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-214 AND SER-226, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	Z23117 Genomic DNA. Translation: CAA80663.1. L29347 Genomic DNA. Translation: AAA53399.1. Z49540 Genomic DNA. Translation: CAA89567.1. L36344 Genomic DNA. Translation: AAA88741.1.
PIR	S50054.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:3949N.
IntAct	P37020. 7 interactions.
Protein family/group databases
TCDB	2.A.49.1.1. chloride carrier/channel (ClC) family.
Genome annotation databases
Ensembl	YJR040W. Saccharomyces cerevisiae. [Contig view]
GenomeReviews	Gene locus YJR040W in contig Y13136_GR.
KEGG	sce:YJR040W.
Organism-specific databases
CYGD	YJR040w.
SGD	S000003801. GEF1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P37020.
Gene expression databases
ArrayExpress	P37020.
GermOnline	YJR040W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR014743. Cl-channel_core. IPR001807. Cl-channel_volt. IPR000644. Cysta_beta_synth_core. [Graphical view]
Gene3D	G3DSA:1.10.3080.10. Cl-channel_core. 1 hit.
PANTHER	PTHR11689. Cl-channel_volt. 1 hit.
Pfam	PF00571. CBS. 1 hit. PF00654. Voltage_CLC. 1 hit. [Graphical view]
PRINTS	PR00762. CLCHANNEL.
SMART	SM00116. CBS. 2 hits. [Graphical view]
PROSITE	PS51371. CBS. 2 hits. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

GEF1_YEAST

Accession

Primary (citable) accession number: P37020

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	June 16, 2009
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents