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Protein

Anion/proton exchange transporter GEF1

Gene

GEF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Anion/proton exchange transporter involved in iron and copper cation homeostasis. Involved in intracellular iron metabolism during growth on fermentable and non fermentable carbon sources. Required for proper copper-loading and maturation of multicopper oxidase FET3. Important for adjusting intracellular compartment pH to more alkaline pH under iron limitation. May also transport chloride ions through the plasma membrane.5 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei230Mediates proton transfer from the outer aqueous phase to the interior of the protein; involved in linking H(+) and Cl(-) transportBy similarity1
Sitei287Mediates proton transfer from the protein to the inner aqueous phaseBy similarity1

GO - Molecular functioni

  • voltage-gated chloride channel activity Source: SGD

GO - Biological processi

  • cellular copper ion homeostasis Source: SGD
  • cellular iron ion homeostasis Source: SGD
  • regulation of iron ion transport Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Enzyme and pathway databases

BioCyciYEAST:G3O-31675-MONOMER.
ReactomeiR-SCE-2672351. Stimuli-sensing channels.

Protein family/group databases

TCDBi2.A.49.1.1. the chloride carrier/channel (clc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Anion/proton exchange transporter GEF1
Alternative name(s):
CLC protein GEF1
ClC-A
ClC-Y1
Voltage-gated chloride channel
Cleaved into the following 2 chains:
Gene namesi
Name:GEF1
Synonyms:CLCY1
Ordered Locus Names:YJR040W
ORF Names:J1616
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR040W.
SGDiS000003801. GEF1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 75CytoplasmicSequence analysisAdd BLAST75
Transmembranei76 – 96HelicalSequence analysisAdd BLAST21
Topological domaini97 – 154LumenalSequence analysisAdd BLAST58
Transmembranei155 – 175HelicalSequence analysisAdd BLAST21
Topological domaini176 – 177CytoplasmicSequence analysis2
Transmembranei178 – 198HelicalSequence analysisAdd BLAST21
Topological domaini199 – 203LumenalSequence analysis5
Transmembranei204 – 224HelicalSequence analysisAdd BLAST21
Topological domaini225 – 264CytoplasmicSequence analysisAdd BLAST40
Transmembranei265 – 285HelicalSequence analysisAdd BLAST21
Topological domaini286 – 296LumenalSequence analysisAdd BLAST11
Transmembranei297 – 319HelicalSequence analysisAdd BLAST23
Topological domaini320 – 336CytoplasmicSequence analysisAdd BLAST17
Transmembranei337 – 357HelicalSequence analysisAdd BLAST21
Topological domaini358 – 369LumenalSequence analysisAdd BLAST12
Transmembranei370 – 390HelicalSequence analysisAdd BLAST21
Topological domaini391 – 436CytoplasmicSequence analysisAdd BLAST46
Transmembranei437 – 457HelicalSequence analysisAdd BLAST21
Topological domaini458 – 465LumenalSequence analysis8
Transmembranei466 – 486HelicalSequence analysisAdd BLAST21
Topological domaini487 – 500CytoplasmicSequence analysisAdd BLAST14
Transmembranei501 – 523HelicalSequence analysisAdd BLAST23
Topological domaini524 – 529LumenalSequence analysis6
Transmembranei530 – 552HelicalSequence analysisAdd BLAST23
Topological domaini553 – 779CytoplasmicSequence analysisAdd BLAST227

GO - Cellular componenti

  • chloride channel complex Source: UniProtKB-KW
  • endoplasmic reticulum Source: SGD
  • endosome Source: SGD
  • fungal-type vacuole Source: SGD
  • Golgi apparatus Source: SGD
  • Golgi medial cisterna Source: SGD
  • Golgi membrane Source: UniProtKB-SubCell
  • late endosome membrane Source: UniProtKB-SubCell
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000944721 – 779Anion/proton exchange transporter GEF1Add BLAST779
ChainiPRO_00004192701 – 136GEF1 N-terminalAdd BLAST136
ChainiPRO_0000419271137 – 779GEF1 C-terminalAdd BLAST643

Post-translational modificationi

Proteolytically processed in the secretory pathway by protease KEX2 within the first extracellular loop. However, both the N- and C-terminal products of the cleavage reaction are required for assembly of a functional channel.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei136 – 137Cleavage; by KEX22

Proteomic databases

MaxQBiP37020.
PRIDEiP37020.

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with GET3.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GET3Q121546EBI-7552,EBI-2989

Protein-protein interaction databases

BioGridi33791. 68 interactors.
DIPiDIP-3949N.
IntActiP37020. 6 interactors.
MINTiMINT-492781.

Structurei

3D structure databases

ProteinModelPortaliP37020.
SMRiP37020.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini591 – 659CBS 1PROSITE-ProRule annotationAdd BLAST69
Domaini688 – 744CBS 2PROSITE-ProRule annotationAdd BLAST57

Sequence similaritiesi

Contains 2 CBS domains.PROSITE-ProRule annotation

Keywords - Domaini

CBS domain, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00760000119109.
HOGENOMiHOG000164493.
InParanoidiP37020.
KOiK05012.
OMAiCQLDENT.
OrthoDBiEOG092C14Y0.

Family and domain databases

Gene3Di1.10.3080.10. 2 hits.
InterProiIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
[Graphical view]
PfamiPF00571. CBS. 2 hits.
PF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
SUPFAMiSSF81340. SSF81340. 3 hits.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37020-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTTYVPINQ PIGDGEDVID TNRFTNIPET QNFDQFVTID KIAEENRPLS
60 70 80 90 100
VDSDREFLNS KYRHYREVIW DRAKTFITLS STAIVIGCIA GFLQVFTETL
110 120 130 140 150
VNWKTGHCQR NWLLNKSFCC NGVVNEVTST SNLLLKRQEF ECEAQGLWIA
160 170 180 190 200
WKGHVSPFII FMLLSVLFAL ISTLLVKYVA PMATGSGISE IKVWVSGFEY
210 220 230 240 250
NKEFLGFLTL VIKSVALPLA ISSGLSVGKE GPSVHYATCC GYLLTKWLLR
260 270 280 290 300
DTLTYSSQYE YITAASGAGV AVAFGAPIGG VLFGLEEIAS ANRFNSSTLW
310 320 330 340 350
KSYYVALVAI TTLKYIDPFR NGRVILFNVT YDRDWKVQEI PIFIALGIFG
360 370 380 390 400
GLYGKYISKW NINFIHFRKM YLSSWPVQEV LFLATLTALI SYFNEFLKLD
410 420 430 440 450
MTESMGILFH ECVKNDNTST FSHRLCQLDE NTHAFEFLKI FTSLCFATVI
460 470 480 490 500
RALLVVVSYG ARVPAGIFVP SMAVGATFGR AVSLLVERFI SGPSVITPGA
510 520 530 540 550
YAFLGAAATL SGITNLTLTV VVIMFELTGA FMYIIPLMIV VAITRIILST
560 570 580 590 600
SGISGGIADQ MIMVNGFPYL EDEQDEEEEE TLEKYTAEQL MSSKLITINE
610 620 630 640 650
TIYLSELESL LYDSASEYSV HGFPITKDED KFEKEKRCIG YVLKRHLASK
660 670 680 690 700
IMMQSVNSTK AQTTLVYFNK SNEELGHREN CIGFKDIMNE SPISVKKAVP
710 720 730 740 750
VTLLFRMFKE LGCKTIIVEE SGILKGLVTA KDILRFKRIK YREVHGAKFT
760 770
YNEALDRRCW SVIHFIIKRF TTNRNGNVI
Length:779
Mass (Da):87,592
Last modified:October 5, 2010 - v2
Checksum:i544C751402D32E19
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13G → R in CAA80663 (PubMed:7505388).Curated1
Sequence conflicti207F → L in CAA80663 (PubMed:7505388).Curated1
Sequence conflicti257S → T in CAA80663 (PubMed:7505388).Curated1
Sequence conflicti262I → L in CAA80663 (PubMed:7505388).Curated1
Sequence conflicti497T → I in CAA80663 (PubMed:7505388).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z23117 Genomic DNA. Translation: CAA80663.1.
L29347 Genomic DNA. Translation: AAA53399.1.
Z49540 Genomic DNA. Translation: CAA89567.1.
L36344 Genomic DNA. Translation: AAA88741.1.
BK006943 Genomic DNA. Translation: DAA08827.1.
PIRiS50054.
RefSeqiNP_012574.1. NM_001181698.1.

Genome annotation databases

EnsemblFungiiYJR040W; YJR040W; YJR040W.
GeneIDi853497.
KEGGisce:YJR040W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z23117 Genomic DNA. Translation: CAA80663.1.
L29347 Genomic DNA. Translation: AAA53399.1.
Z49540 Genomic DNA. Translation: CAA89567.1.
L36344 Genomic DNA. Translation: AAA88741.1.
BK006943 Genomic DNA. Translation: DAA08827.1.
PIRiS50054.
RefSeqiNP_012574.1. NM_001181698.1.

3D structure databases

ProteinModelPortaliP37020.
SMRiP37020.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33791. 68 interactors.
DIPiDIP-3949N.
IntActiP37020. 6 interactors.
MINTiMINT-492781.

Protein family/group databases

TCDBi2.A.49.1.1. the chloride carrier/channel (clc) family.

Proteomic databases

MaxQBiP37020.
PRIDEiP37020.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR040W; YJR040W; YJR040W.
GeneIDi853497.
KEGGisce:YJR040W.

Organism-specific databases

EuPathDBiFungiDB:YJR040W.
SGDiS000003801. GEF1.

Phylogenomic databases

GeneTreeiENSGT00760000119109.
HOGENOMiHOG000164493.
InParanoidiP37020.
KOiK05012.
OMAiCQLDENT.
OrthoDBiEOG092C14Y0.

Enzyme and pathway databases

BioCyciYEAST:G3O-31675-MONOMER.
ReactomeiR-SCE-2672351. Stimuli-sensing channels.

Miscellaneous databases

PROiP37020.

Family and domain databases

Gene3Di1.10.3080.10. 2 hits.
InterProiIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
[Graphical view]
PfamiPF00571. CBS. 2 hits.
PF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
SUPFAMiSSF81340. SSF81340. 3 hits.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGEF1_YEAST
AccessioniPrimary (citable) accession number: P37020
Secondary accession number(s): D6VWL1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: October 5, 2010
Last modified: November 2, 2016
This is version 159 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be a voltage-gated ClC-type chloride channel.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.