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P37020

- GEF1_YEAST

UniProt

P37020 - GEF1_YEAST

Protein

Anion/proton exchange transporter GEF1

Gene

GEF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 140 (01 Oct 2014)
      Sequence version 2 (05 Oct 2010)
      Previous versions | rss
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    Functioni

    Anion/proton exchange transporter involved in iron and copper cation homeostasis. Involved in intracellular iron metabolism during growth on fermentable and non fermentable carbon sources. Required for proper copper-loading and maturation of multicopper oxidase FET3. Important for adjusting intracellular compartment pH to more alkaline pH under iron limitation. May also transport chloride ions through the plasma membrane.5 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei136 – 1372Cleavage; by KEX2
    Sitei230 – 2301Mediates proton transfer from the outer aqueous phase to the interior of the protein; involved in linking H(+) and Cl(-) transportBy similarity
    Sitei287 – 2871Mediates proton transfer from the protein to the inner aqueous phaseBy similarity

    GO - Molecular functioni

    1. adenyl nucleotide binding Source: InterPro
    2. protein binding Source: IntAct
    3. voltage-gated chloride channel activity Source: SGD

    GO - Biological processi

    1. cellular copper ion homeostasis Source: SGD
    2. cellular iron ion homeostasis Source: SGD
    3. regulation of anion transport Source: GOC

    Keywords - Molecular functioni

    Chloride channel, Ion channel

    Keywords - Biological processi

    Ion transport, Transport

    Keywords - Ligandi

    Chloride

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31675-MONOMER.
    ReactomeiREACT_204366. Stimuli-sensing channels.

    Protein family/group databases

    TCDBi2.A.49.1.1. the chloride carrier/channel (clc) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Anion/proton exchange transporter GEF1
    Alternative name(s):
    CLC protein GEF1
    ClC-A
    ClC-Y1
    Voltage-gated chloride channel
    Cleaved into the following 2 chains:
    Gene namesi
    Name:GEF1
    Synonyms:CLCY1
    Ordered Locus Names:YJR040W
    ORF Names:J1616
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    SGDiS000003801. GEF1.

    Subcellular locationi

    GO - Cellular componenti

    1. chloride channel complex Source: UniProtKB-KW
    2. endoplasmic reticulum Source: SGD
    3. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    4. endosome Source: SGD
    5. endosome membrane Source: UniProtKB-SubCell
    6. fungal-type vacuole Source: SGD
    7. Golgi apparatus Source: SGD
    8. Golgi medial cisterna Source: SGD
    9. Golgi membrane Source: UniProtKB-SubCell
    10. plasma membrane Source: SGD
    11. vacuolar membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane, Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 779779Anion/proton exchange transporter GEF1PRO_0000094472Add
    BLAST
    Chaini1 – 136136GEF1 N-terminalPRO_0000419270Add
    BLAST
    Chaini137 – 779643GEF1 C-terminalPRO_0000419271Add
    BLAST

    Post-translational modificationi

    Proteolytically processed in the secretory pathway by protease KEX2 within the first extracellular loop. However, both the N- and C-terminal products of the cleavage reaction are required for assembly of a functional channel.1 Publication

    Proteomic databases

    MaxQBiP37020.
    PaxDbiP37020.

    Expressioni

    Gene expression databases

    GenevestigatoriP37020.

    Interactioni

    Subunit structurei

    Homodimer By similarity. Interacts with GET3.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    GET3Q121546EBI-7552,EBI-2989

    Protein-protein interaction databases

    BioGridi33791. 66 interactions.
    DIPiDIP-3949N.
    IntActiP37020. 6 interactions.
    MINTiMINT-492781.
    STRINGi4932.YJR040W.

    Structurei

    3D structure databases

    ProteinModelPortaliP37020.
    SMRiP37020. Positions 153-570, 596-735.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 7575CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini97 – 15458LumenalSequence AnalysisAdd
    BLAST
    Topological domaini176 – 1772CytoplasmicSequence Analysis
    Topological domaini199 – 2035LumenalSequence Analysis
    Topological domaini225 – 26440CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini286 – 29611LumenalSequence AnalysisAdd
    BLAST
    Topological domaini320 – 33617CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini358 – 36912LumenalSequence AnalysisAdd
    BLAST
    Topological domaini391 – 43646CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini458 – 4658LumenalSequence Analysis
    Topological domaini487 – 50014CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini524 – 5296LumenalSequence Analysis
    Topological domaini553 – 779227CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei76 – 9621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei155 – 17521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei178 – 19821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei204 – 22421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei265 – 28521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei297 – 31923HelicalSequence AnalysisAdd
    BLAST
    Transmembranei337 – 35721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei370 – 39021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei437 – 45721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei466 – 48621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei501 – 52323HelicalSequence AnalysisAdd
    BLAST
    Transmembranei530 – 55223HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini591 – 65969CBS 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini688 – 74457CBS 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 2 CBS domains.PROSITE-ProRule annotation

    Keywords - Domaini

    CBS domain, Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0038.
    GeneTreeiENSGT00620000087693.
    HOGENOMiHOG000164493.
    KOiK05012.
    OrthoDBiEOG72RN6P.

    Family and domain databases

    Gene3Di1.10.3080.10. 2 hits.
    InterProiIPR000644. CBS_dom.
    IPR014743. Cl-channel_core.
    IPR001807. Cl-channel_volt-gated.
    [Graphical view]
    PfamiPF00571. CBS. 2 hits.
    PF00654. Voltage_CLC. 1 hit.
    [Graphical view]
    PRINTSiPR00762. CLCHANNEL.
    SMARTiSM00116. CBS. 2 hits.
    [Graphical view]
    SUPFAMiSSF81340. SSF81340. 3 hits.
    PROSITEiPS51371. CBS. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P37020-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPTTYVPINQ PIGDGEDVID TNRFTNIPET QNFDQFVTID KIAEENRPLS    50
    VDSDREFLNS KYRHYREVIW DRAKTFITLS STAIVIGCIA GFLQVFTETL 100
    VNWKTGHCQR NWLLNKSFCC NGVVNEVTST SNLLLKRQEF ECEAQGLWIA 150
    WKGHVSPFII FMLLSVLFAL ISTLLVKYVA PMATGSGISE IKVWVSGFEY 200
    NKEFLGFLTL VIKSVALPLA ISSGLSVGKE GPSVHYATCC GYLLTKWLLR 250
    DTLTYSSQYE YITAASGAGV AVAFGAPIGG VLFGLEEIAS ANRFNSSTLW 300
    KSYYVALVAI TTLKYIDPFR NGRVILFNVT YDRDWKVQEI PIFIALGIFG 350
    GLYGKYISKW NINFIHFRKM YLSSWPVQEV LFLATLTALI SYFNEFLKLD 400
    MTESMGILFH ECVKNDNTST FSHRLCQLDE NTHAFEFLKI FTSLCFATVI 450
    RALLVVVSYG ARVPAGIFVP SMAVGATFGR AVSLLVERFI SGPSVITPGA 500
    YAFLGAAATL SGITNLTLTV VVIMFELTGA FMYIIPLMIV VAITRIILST 550
    SGISGGIADQ MIMVNGFPYL EDEQDEEEEE TLEKYTAEQL MSSKLITINE 600
    TIYLSELESL LYDSASEYSV HGFPITKDED KFEKEKRCIG YVLKRHLASK 650
    IMMQSVNSTK AQTTLVYFNK SNEELGHREN CIGFKDIMNE SPISVKKAVP 700
    VTLLFRMFKE LGCKTIIVEE SGILKGLVTA KDILRFKRIK YREVHGAKFT 750
    YNEALDRRCW SVIHFIIKRF TTNRNGNVI 779
    Length:779
    Mass (Da):87,592
    Last modified:October 5, 2010 - v2
    Checksum:i544C751402D32E19
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti13 – 131G → R in CAA80663. (PubMed:7505388)Curated
    Sequence conflicti207 – 2071F → L in CAA80663. (PubMed:7505388)Curated
    Sequence conflicti257 – 2571S → T in CAA80663. (PubMed:7505388)Curated
    Sequence conflicti262 – 2621I → L in CAA80663. (PubMed:7505388)Curated
    Sequence conflicti497 – 4971T → I in CAA80663. (PubMed:7505388)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z23117 Genomic DNA. Translation: CAA80663.1.
    L29347 Genomic DNA. Translation: AAA53399.1.
    Z49540 Genomic DNA. Translation: CAA89567.1.
    L36344 Genomic DNA. Translation: AAA88741.1.
    BK006943 Genomic DNA. Translation: DAA08827.1.
    PIRiS50054.
    RefSeqiNP_012574.1. NM_001181698.1.

    Genome annotation databases

    EnsemblFungiiYJR040W; YJR040W; YJR040W.
    GeneIDi853497.
    KEGGisce:YJR040W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z23117 Genomic DNA. Translation: CAA80663.1 .
    L29347 Genomic DNA. Translation: AAA53399.1 .
    Z49540 Genomic DNA. Translation: CAA89567.1 .
    L36344 Genomic DNA. Translation: AAA88741.1 .
    BK006943 Genomic DNA. Translation: DAA08827.1 .
    PIRi S50054.
    RefSeqi NP_012574.1. NM_001181698.1.

    3D structure databases

    ProteinModelPortali P37020.
    SMRi P37020. Positions 153-570, 596-735.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33791. 66 interactions.
    DIPi DIP-3949N.
    IntActi P37020. 6 interactions.
    MINTi MINT-492781.
    STRINGi 4932.YJR040W.

    Protein family/group databases

    TCDBi 2.A.49.1.1. the chloride carrier/channel (clc) family.

    Proteomic databases

    MaxQBi P37020.
    PaxDbi P37020.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJR040W ; YJR040W ; YJR040W .
    GeneIDi 853497.
    KEGGi sce:YJR040W.

    Organism-specific databases

    SGDi S000003801. GEF1.

    Phylogenomic databases

    eggNOGi COG0038.
    GeneTreei ENSGT00620000087693.
    HOGENOMi HOG000164493.
    KOi K05012.
    OrthoDBi EOG72RN6P.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31675-MONOMER.
    Reactomei REACT_204366. Stimuli-sensing channels.

    Miscellaneous databases

    NextBioi 974134.

    Gene expression databases

    Genevestigatori P37020.

    Family and domain databases

    Gene3Di 1.10.3080.10. 2 hits.
    InterProi IPR000644. CBS_dom.
    IPR014743. Cl-channel_core.
    IPR001807. Cl-channel_volt-gated.
    [Graphical view ]
    Pfami PF00571. CBS. 2 hits.
    PF00654. Voltage_CLC. 1 hit.
    [Graphical view ]
    PRINTSi PR00762. CLCHANNEL.
    SMARTi SM00116. CBS. 2 hits.
    [Graphical view ]
    SUPFAMi SSF81340. SSF81340. 3 hits.
    PROSITEi PS51371. CBS. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The GEF1 gene of Saccharomyces cerevisiae encodes an integral membrane protein; mutations in which have effects on respiration and iron-limited growth."
      Greene J.R., Brown N.H., Didomenico B.J., Kaplan J., Eide D.J.
      Mol. Gen. Genet. 241:542-553(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
      Strain: ATCC 204508 / S288c.
    2. "A voltage-gated chloride channel in the yeast Saccharomyces cerevisiae."
      Huang M.-E., Chuat J.-C., Galibert F.
      J. Mol. Biol. 242:595-598(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Analysis of a 42.5 kb DNA sequence of chromosome X reveals three tRNA genes and 14 new open reading frames including a gene most probably belonging to the family of ubiquitin-protein ligases."
      Huang M.-E., Chuat J.-C., Galibert F.
      Yeast 11:775-781(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "Golgi localization and functionally important domains in the NH2 and COOH terminus of the yeast CLC putative chloride channel Gef1p."
      Schwappach B., Stobrawa S., Hechenberger M., Steinmeyer K., Jentsch T.J.
      J. Biol. Chem. 273:15110-15118(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    7. "The yeast CLC chloride channel functions in cation homeostasis."
      Gaxiola R.A., Yuan D.S., Klausner R.D., Fink G.R.
      Proc. Natl. Acad. Sci. U.S.A. 95:4046-4050(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    8. "The Gef1 protein of Saccharomyces cerevisiae is associated with chloride channel activity."
      Flis K., Bednarczyk P., Hordejuk R., Szewczyk A., Berest V., Dolowy K., Edelman A., Kurlandzka A.
      Biochem. Biophys. Res. Commun. 294:1144-1150(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "The yeast CLC chloride channel is proteolytically processed by the furin-like protease Kex2p in the first extracellular loop."
      Wachter A., Schwappach B.
      FEBS Lett. 579:1149-1153(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: CLEAVAGE BY KEX2, SUBCELLULAR LOCATION.
    10. "The yeast Arr4p ATPase binds the chloride transporter Gef1p when copper is available in the cytosol."
      Metz J., Waechter A., Schmidt B., Bujnicki J.M., Schwappach B.
      J. Biol. Chem. 281:410-417(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GET3.
    11. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    12. "The product of the gene GEF1 of Saccharomyces cerevisiae transports Cl- across the plasma membrane."
      Lopez-Rodriguez A., Trejo A.C., Coyne L., Halliwell R.F., Miledi R., Martinez-Torres A.
      FEMS Yeast Res. 7:1218-1229(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiGEF1_YEAST
    AccessioniPrimary (citable) accession number: P37020
    Secondary accession number(s): D6VWL1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: October 5, 2010
    Last modified: October 1, 2014
    This is version 140 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Caution

    Was originally thought to be a voltage-gated ClC-type chloride channel.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3