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Protein

H(+)/Cl(-) exchange transporter ClcA

Gene

clcA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme acid resistance (XAR) response.5 Publications

Caution

Was originally thought to be a voltage-gated ClC-type chloride channel.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei107Chloride1
Sitei148Mediates proton transfer from the outer aqueous phase to the interior of the protein; involved in linking H(+) and Cl(-) transport1
Sitei203Mediates proton transfer from the protein to the inner aqueous phase1
Binding sitei356Chloride; via amide nitrogen1
Binding sitei357Chloride; via amide nitrogen1
Binding sitei445Chloride1

GO - Molecular functioni

  • anion binding Source: EcoCyc
  • chloride ion binding Source: EcoCyc
  • identical protein binding Source: IntAct
  • solute:proton antiporter activity Source: EcoCyc
  • voltage-gated chloride channel activity Source: InterPro

GO - Biological processi

  • chloride transport Source: EcoCyc
  • nitrate transport Source: EcoCyc

Keywordsi

Biological processAntiport, Ion transport, Stress response, Transport
LigandChloride

Enzyme and pathway databases

BioCyciEcoCyc:YADQ-MONOMER
MetaCyc:YADQ-MONOMER

Protein family/group databases

TCDBi2.A.49.5.1 the chloride carrier/channel (clc) family

Names & Taxonomyi

Protein namesi
Recommended name:
H(+)/Cl(-) exchange transporter ClcA
Alternative name(s):
ClC-ec1
Gene namesi
Name:clcA
Synonyms:eriC, yadQ
Ordered Locus Names:b0155, JW5012
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12331 clcA

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 32CytoplasmicAdd BLAST32
Transmembranei33 – 69HelicalAdd BLAST37
Topological domaini70 – 76Periplasmic7
Transmembranei77 – 100HelicalAdd BLAST24
Intramembranei109 – 116Helical8
Topological domaini117 – 123Cytoplasmic7
Transmembranei124 – 141HelicalAdd BLAST18
Transmembranei148 – 166HelicalAdd BLAST19
Topological domaini167 – 176Cytoplasmic10
Intramembranei177 – 189HelicalAdd BLAST13
Intramembranei193 – 201Helical9
Topological domaini202 – 214CytoplasmicAdd BLAST13
Transmembranei215 – 232HelicalAdd BLAST18
Topological domaini233 – 252PeriplasmicAdd BLAST20
Transmembranei253 – 281HelicalAdd BLAST29
Topological domaini282 – 287Cytoplasmic6
Transmembranei288 – 309HelicalAdd BLAST22
Topological domaini310 – 329PeriplasmicAdd BLAST20
Transmembranei330 – 349HelicalAdd BLAST20
Transmembranei355 – 376HelicalAdd BLAST22
Topological domaini377 – 386Periplasmic10
Intramembranei387 – 401HelicalAdd BLAST15
Intramembranei402 – 404Note=Loop between two helices3
Intramembranei405 – 416HelicalAdd BLAST12
Intramembranei417 – 421Note=Loop between two helices5
Transmembranei422 – 438HelicalAdd BLAST17
Topological domaini439 – 473CytoplasmicAdd BLAST35

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi107S → A: Uncouples chloride transport from proton transport. 1 Publication1
Mutagenesisi148E → A or Q: Abolishes proton transport, but permits the transit of chloride ions. Abolishes gating, permitting continuous rapid transit of chloride ions; when associated with A-445. 4 Publications1
Mutagenesisi203E → A, G, Q, S or T: Abolishes proton transport, and reduces chloride transport. 1 Publication1
Mutagenesisi203E → C, I, L or V: Abolishes proton and chloride transport. 1 Publication1
Mutagenesisi203E → D or H: No effect on proton and chloride transport. 1 Publication1
Mutagenesisi203E → K or R: Decreased proton and chloride transport. 1 Publication1
Mutagenesisi445Y → A: Abolishes gating, permitting continuous rapid transit of chloride ions; when associated with A-148. 3 Publications1
Mutagenesisi445Y → F or W: No effect. 3 Publications1
Mutagenesisi445Y → L: Alters stoichiometry of proton/chloride exchange. 3 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000944731 – 473H(+)/Cl(-) exchange transporter ClcAAdd BLAST473

Proteomic databases

PaxDbiP37019
PRIDEiP37019

Expressioni

Inductioni

By acid-shock conditions.

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself7EBI-15719361,EBI-15719361

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4259368, 6 interactors
DIPiDIP-9523N
STRINGi316385.ECDH10B_0135

Structurei

Secondary structure

1473
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi18 – 26Combined sources9
Turni27 – 29Combined sources3
Helixi33 – 70Combined sources38
Beta strandi72 – 74Combined sources3
Helixi75 – 100Combined sources26
Helixi102 – 104Combined sources3
Helixi109 – 116Combined sources8
Helixi124 – 140Combined sources17
Helixi148 – 165Combined sources18
Helixi171 – 190Combined sources20
Helixi193 – 202Combined sources10
Beta strandi203 – 205Combined sources3
Beta strandi207 – 209Combined sources3
Helixi215 – 231Combined sources17
Turni232 – 235Combined sources4
Helixi249 – 251Combined sources3
Helixi252 – 284Combined sources33
Helixi288 – 308Combined sources21
Helixi310 – 312Combined sources3
Beta strandi313 – 316Combined sources4
Helixi319 – 324Combined sources6
Helixi330 – 348Combined sources19
Turni349 – 351Combined sources3
Beta strandi353 – 356Combined sources4
Helixi357 – 378Combined sources22
Helixi380 – 382Combined sources3
Helixi386 – 394Combined sources9
Helixi396 – 401Combined sources6
Helixi405 – 416Combined sources12
Helixi419 – 421Combined sources3
Helixi422 – 438Combined sources17
Helixi444 – 458Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KPKX-ray3.50A/B/C/D/E/F1-473[»]
1OTSX-ray2.51A/B1-465[»]
1OTTX-ray3.00A/B1-465[»]
1OTUX-ray3.30A/B1-465[»]
2EXWX-ray3.20A/B1-473[»]
2EXYX-ray3.10A/B1-473[»]
2EZ0X-ray3.54A/B1-473[»]
2FECX-ray3.97A/B1-465[»]
2FEDX-ray3.32A/B1-465[»]
2FEEX-ray3.20A/B1-465[»]
2H2PX-ray3.10A/B1-465[»]
2H2SX-ray3.10A/B1-465[»]
2HLFX-ray3.30A/B17-460[»]
2HT2X-ray3.32A/B1-473[»]
2HT3X-ray3.30A/B1-473[»]
2HT4X-ray3.20A/B1-473[»]
2HTKX-ray3.41A/B1-473[»]
2HTLX-ray3.40A/B1-473[»]
2R9HX-ray3.10A/B17-460[»]
3DETX-ray2.80A/B1-473[»]
3EJYX-ray3.20A/B1-473[»]
3EJZX-ray2.90A/B1-473[»]
3NMOX-ray3.10A1-465[»]
4ENEX-ray2.40A/B17-460[»]
4FTPX-ray3.21A1-465[»]
4KJPX-ray3.20A/B17-460[»]
4KJQX-ray2.88A/B17-460[»]
4KJWX-ray3.03A/B17-460[»]
4KK5X-ray3.17A/B17-460[»]
4KK6X-ray3.18A/B17-460[»]
4KK8X-ray2.86A/B17-460[»]
4KK9X-ray3.00A/B17-460[»]
4KKAX-ray3.00A/B17-460[»]
4KKBX-ray3.02A/B17-460[»]
4KKCX-ray3.18A/B17-460[»]
4KKLX-ray2.85A/B17-460[»]
4LOUX-ray2.98A/B17-460[»]
4MQXX-ray3.52A/B1-465[»]
5HD8X-ray3.15A/B17-465[»]
ProteinModelPortaliP37019
SMRiP37019
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37019

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi106 – 110Selectivity filter part_15
Motifi146 – 150Selectivity filter part_25
Motifi355 – 359Selectivity filter part_35

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CMQ Bacteria
COG0038 LUCA
HOGENOMiHOG000257546
InParanoidiP37019
KOiK03281
OMAiEGPTAQF
PhylomeDBiP37019

Family and domain databases

Gene3Di1.10.3080.10, 1 hit
HAMAPiMF_01128 CLC_ClcA, 1 hit
InterProiView protein in InterPro
IPR023861 Cl-channel_ClcA
IPR014743 Cl-channel_core
IPR001807 Cl-channel_volt-gated
PfamiView protein in Pfam
PF00654 Voltage_CLC, 1 hit
PRINTSiPR00762 CLCHANNEL
SUPFAMiSSF81340 SSF81340, 1 hit

Sequencei

Sequence statusi: Complete.

P37019-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTDTPSLET PQAARLRRRQ LIRQLLERDK TPLAILFMAA VVGTLVGLAA
60 70 80 90 100
VAFDKGVAWL QNQRMGALVH TADNYPLLLT VAFLCSAVLA MFGYFLVRKY
110 120 130 140 150
APEAGGSGIP EIEGALEDQR PVRWWRVLPV KFFGGLGTLG GGMVLGREGP
160 170 180 190 200
TVQIGGNIGR MVLDIFRLKG DEARHTLLAT GAAAGLAAAF NAPLAGILFI
210 220 230 240 250
IEEMRPQFRY TLISIKAVFI GVIMSTIMYR IFNHEVALID VGKLSDAPLN
260 270 280 290 300
TLWLYLILGI IFGIFGPIFN KWVLGMQDLL HRVHGGNITK WVLMGGAIGG
310 320 330 340 350
LCGLLGFVAP ATSGGGFNLI PIATAGNFSM GMLVFIFVAR VITTLLCFSS
360 370 380 390 400
GAPGGIFAPM LALGTVLGTA FGMVAVELFP QYHLEAGTFA IAGMGALLAA
410 420 430 440 450
SIRAPLTGII LVLEMTDNYQ LILPMIITGL GATLLAQFTG GKPLYSAILA
460 470
RTLAKQEAEQ LARSKAASAS ENT
Length:473
Mass (Da):50,349
Last modified:April 27, 2001 - v2
Checksum:i0B1BC39B417E9690
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti32P → Q (PubMed:8202364).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70214 Genomic DNA Translation: AAB08585.1
U00096 Genomic DNA Translation: AAC73266.1
AP009048 Genomic DNA Translation: BAB96732.2
PIRiC64739
RefSeqiNP_414697.1, NC_000913.3
WP_000845394.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73266; AAC73266; b0155
BAB96732; BAB96732; BAB96732
GeneIDi946715
KEGGiecj:JW5012
eco:b0155
PATRICifig|1411691.4.peg.2125

Similar proteinsi

Entry informationi

Entry nameiCLCA_ECOLI
AccessioniPrimary (citable) accession number: P37019
Secondary accession number(s): P77394
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: April 27, 2001
Last modified: March 28, 2018
This is version 167 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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