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Protein

H(+)/Cl(-) exchange transporter ClcA

Gene

clcA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme acid resistance (XAR) response.5 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei107Chloride1
Sitei148Mediates proton transfer from the outer aqueous phase to the interior of the protein; involved in linking H(+) and Cl(-) transport1
Sitei203Mediates proton transfer from the protein to the inner aqueous phase1
Binding sitei356Chloride; via amide nitrogen1
Binding sitei357Chloride; via amide nitrogen1
Binding sitei445Chloride1

GO - Molecular functioni

  • anion binding Source: EcoCyc
  • chloride ion binding Source: EcoCyc
  • solute:proton antiporter activity Source: EcoCyc
  • voltage-gated chloride channel activity Source: GO_Central

GO - Biological processi

  • chloride transmembrane transport Source: GO_Central
  • chloride transport Source: EcoCyc
  • nitrate transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Antiport, Ion transport, Stress response, Transport

Keywords - Ligandi

Chloride

Enzyme and pathway databases

BioCyciEcoCyc:YADQ-MONOMER.
ECOL316407:JW5012-MONOMER.
MetaCyc:YADQ-MONOMER.

Protein family/group databases

TCDBi2.A.49.5.1. the chloride carrier/channel (clc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
H(+)/Cl(-) exchange transporter ClcA
Alternative name(s):
ClC-ec1
Gene namesi
Name:clcA
Synonyms:eriC, yadQ
Ordered Locus Names:b0155, JW5012
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12331. clcA.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 32CytoplasmicAdd BLAST32
Transmembranei33 – 69HelicalAdd BLAST37
Topological domaini70 – 76Periplasmic7
Transmembranei77 – 100HelicalAdd BLAST24
Intramembranei109 – 116Helical8
Topological domaini117 – 123Cytoplasmic7
Transmembranei124 – 141HelicalAdd BLAST18
Transmembranei148 – 166HelicalAdd BLAST19
Topological domaini167 – 176Cytoplasmic10
Intramembranei177 – 189HelicalAdd BLAST13
Intramembranei193 – 201Helical9
Topological domaini202 – 214CytoplasmicAdd BLAST13
Transmembranei215 – 232HelicalAdd BLAST18
Topological domaini233 – 252PeriplasmicAdd BLAST20
Transmembranei253 – 281HelicalAdd BLAST29
Topological domaini282 – 287Cytoplasmic6
Transmembranei288 – 309HelicalAdd BLAST22
Topological domaini310 – 329PeriplasmicAdd BLAST20
Transmembranei330 – 349HelicalAdd BLAST20
Transmembranei355 – 376HelicalAdd BLAST22
Topological domaini377 – 386Periplasmic10
Intramembranei387 – 401HelicalAdd BLAST15
Intramembranei402 – 404Note=Loop between two helices3
Intramembranei405 – 416HelicalAdd BLAST12
Intramembranei417 – 421Note=Loop between two helices5
Transmembranei422 – 438HelicalAdd BLAST17
Topological domaini439 – 473CytoplasmicAdd BLAST35

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi107S → A: Uncouples chloride transport from proton transport. 1 Publication1
Mutagenesisi148E → A or Q: Abolishes proton transport, but permits the transit of chloride ions. Abolishes gating, permitting continuous rapid transit of chloride ions; when associated with A-445. 4 Publications1
Mutagenesisi203E → A, G, Q, S or T: Abolishes proton transport, and reduces chloride transport. 1 Publication1
Mutagenesisi203E → C, I, L or V: Abolishes proton and chloride transport. 1 Publication1
Mutagenesisi203E → D or H: No effect on proton and chloride transport. 1 Publication1
Mutagenesisi203E → K or R: Decreased proton and chloride transport. 1 Publication1
Mutagenesisi445Y → A: Abolishes gating, permitting continuous rapid transit of chloride ions; when associated with A-148. 3 Publications1
Mutagenesisi445Y → F or W: No effect. 3 Publications1
Mutagenesisi445Y → L: Alters stoichiometry of proton/chloride exchange. 3 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000944731 – 473H(+)/Cl(-) exchange transporter ClcAAdd BLAST473

Proteomic databases

PaxDbiP37019.
PRIDEiP37019.

Expressioni

Inductioni

By acid-shock conditions.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4259368. 1 interactor.
DIPiDIP-9523N.
STRINGi511145.b0155.

Structurei

Secondary structure

1473
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi18 – 26Combined sources9
Turni27 – 29Combined sources3
Helixi33 – 70Combined sources38
Beta strandi72 – 74Combined sources3
Helixi75 – 100Combined sources26
Helixi102 – 104Combined sources3
Helixi109 – 116Combined sources8
Helixi124 – 140Combined sources17
Helixi148 – 165Combined sources18
Helixi171 – 190Combined sources20
Helixi193 – 202Combined sources10
Beta strandi203 – 205Combined sources3
Beta strandi207 – 209Combined sources3
Helixi215 – 231Combined sources17
Turni232 – 235Combined sources4
Helixi249 – 251Combined sources3
Helixi252 – 284Combined sources33
Helixi288 – 308Combined sources21
Helixi310 – 312Combined sources3
Beta strandi313 – 316Combined sources4
Helixi319 – 324Combined sources6
Helixi330 – 348Combined sources19
Turni349 – 351Combined sources3
Beta strandi353 – 356Combined sources4
Helixi357 – 378Combined sources22
Helixi380 – 382Combined sources3
Helixi386 – 394Combined sources9
Helixi396 – 401Combined sources6
Helixi405 – 416Combined sources12
Helixi419 – 421Combined sources3
Helixi422 – 438Combined sources17
Helixi444 – 458Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KPKX-ray3.50A/B/C/D/E/F1-473[»]
1OTSX-ray2.51A/B1-465[»]
1OTTX-ray3.00A/B1-465[»]
1OTUX-ray3.30A/B1-465[»]
2EXWX-ray3.20A/B1-473[»]
2EXYX-ray3.10A/B1-473[»]
2EZ0X-ray3.54A/B1-473[»]
2FECX-ray3.97A/B1-465[»]
2FEDX-ray3.32A/B1-465[»]
2FEEX-ray3.20A/B1-465[»]
2H2PX-ray3.10A/B1-465[»]
2H2SX-ray3.10A/B1-465[»]
2HLFX-ray3.30A/B17-460[»]
2HT2X-ray3.32A/B1-473[»]
2HT3X-ray3.30A/B1-473[»]
2HT4X-ray3.20A/B1-473[»]
2HTKX-ray3.41A/B1-473[»]
2HTLX-ray3.40A/B1-473[»]
2R9HX-ray3.10A/B17-460[»]
3DETX-ray2.80A/B1-473[»]
3EJYX-ray3.20A/B1-473[»]
3EJZX-ray2.90A/B1-473[»]
3NMOX-ray3.10A1-465[»]
4ENEX-ray2.40A/B17-460[»]
4FTPX-ray3.21A1-465[»]
4KJPX-ray3.20A/B17-460[»]
4KJQX-ray2.88A/B17-460[»]
4KJWX-ray3.03A/B17-460[»]
4KK5X-ray3.17A/B17-460[»]
4KK6X-ray3.18A/B17-460[»]
4KK8X-ray2.86A/B17-460[»]
4KK9X-ray3.00A/B17-460[»]
4KKAX-ray3.00A/B17-460[»]
4KKBX-ray3.02A/B17-460[»]
4KKCX-ray3.18A/B17-460[»]
4KKLX-ray2.85A/B17-460[»]
4LOUX-ray2.98A/B17-460[»]
4MQXX-ray3.52A/B1-465[»]
ProteinModelPortaliP37019.
SMRiP37019.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37019.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi106 – 110Selectivity filter part_15
Motifi146 – 150Selectivity filter part_25
Motifi355 – 359Selectivity filter part_35

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CMQ. Bacteria.
COG0038. LUCA.
HOGENOMiHOG000257546.
InParanoidiP37019.
KOiK03281.
OMAiFPAYHLD.
PhylomeDBiP37019.

Family and domain databases

Gene3Di1.10.3080.10. 1 hit.
HAMAPiMF_01128. CLC_ClcA. 1 hit.
InterProiIPR023861. Cl-channel_ClcA.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
[Graphical view]
PfamiPF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
SUPFAMiSSF81340. SSF81340. 1 hit.

Sequencei

Sequence statusi: Complete.

P37019-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTDTPSLET PQAARLRRRQ LIRQLLERDK TPLAILFMAA VVGTLVGLAA
60 70 80 90 100
VAFDKGVAWL QNQRMGALVH TADNYPLLLT VAFLCSAVLA MFGYFLVRKY
110 120 130 140 150
APEAGGSGIP EIEGALEDQR PVRWWRVLPV KFFGGLGTLG GGMVLGREGP
160 170 180 190 200
TVQIGGNIGR MVLDIFRLKG DEARHTLLAT GAAAGLAAAF NAPLAGILFI
210 220 230 240 250
IEEMRPQFRY TLISIKAVFI GVIMSTIMYR IFNHEVALID VGKLSDAPLN
260 270 280 290 300
TLWLYLILGI IFGIFGPIFN KWVLGMQDLL HRVHGGNITK WVLMGGAIGG
310 320 330 340 350
LCGLLGFVAP ATSGGGFNLI PIATAGNFSM GMLVFIFVAR VITTLLCFSS
360 370 380 390 400
GAPGGIFAPM LALGTVLGTA FGMVAVELFP QYHLEAGTFA IAGMGALLAA
410 420 430 440 450
SIRAPLTGII LVLEMTDNYQ LILPMIITGL GATLLAQFTG GKPLYSAILA
460 470
RTLAKQEAEQ LARSKAASAS ENT
Length:473
Mass (Da):50,349
Last modified:April 27, 2001 - v2
Checksum:i0B1BC39B417E9690
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti32P → Q (PubMed:8202364).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70214 Genomic DNA. Translation: AAB08585.1.
U00096 Genomic DNA. Translation: AAC73266.1.
AP009048 Genomic DNA. Translation: BAB96732.2.
PIRiC64739.
RefSeqiNP_414697.1. NC_000913.3.
WP_000845394.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73266; AAC73266; b0155.
BAB96732; BAB96732; BAB96732.
GeneIDi946715.
KEGGiecj:JW5012.
eco:b0155.
PATRICi32115419. VBIEscCol129921_0161.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70214 Genomic DNA. Translation: AAB08585.1.
U00096 Genomic DNA. Translation: AAC73266.1.
AP009048 Genomic DNA. Translation: BAB96732.2.
PIRiC64739.
RefSeqiNP_414697.1. NC_000913.3.
WP_000845394.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KPKX-ray3.50A/B/C/D/E/F1-473[»]
1OTSX-ray2.51A/B1-465[»]
1OTTX-ray3.00A/B1-465[»]
1OTUX-ray3.30A/B1-465[»]
2EXWX-ray3.20A/B1-473[»]
2EXYX-ray3.10A/B1-473[»]
2EZ0X-ray3.54A/B1-473[»]
2FECX-ray3.97A/B1-465[»]
2FEDX-ray3.32A/B1-465[»]
2FEEX-ray3.20A/B1-465[»]
2H2PX-ray3.10A/B1-465[»]
2H2SX-ray3.10A/B1-465[»]
2HLFX-ray3.30A/B17-460[»]
2HT2X-ray3.32A/B1-473[»]
2HT3X-ray3.30A/B1-473[»]
2HT4X-ray3.20A/B1-473[»]
2HTKX-ray3.41A/B1-473[»]
2HTLX-ray3.40A/B1-473[»]
2R9HX-ray3.10A/B17-460[»]
3DETX-ray2.80A/B1-473[»]
3EJYX-ray3.20A/B1-473[»]
3EJZX-ray2.90A/B1-473[»]
3NMOX-ray3.10A1-465[»]
4ENEX-ray2.40A/B17-460[»]
4FTPX-ray3.21A1-465[»]
4KJPX-ray3.20A/B17-460[»]
4KJQX-ray2.88A/B17-460[»]
4KJWX-ray3.03A/B17-460[»]
4KK5X-ray3.17A/B17-460[»]
4KK6X-ray3.18A/B17-460[»]
4KK8X-ray2.86A/B17-460[»]
4KK9X-ray3.00A/B17-460[»]
4KKAX-ray3.00A/B17-460[»]
4KKBX-ray3.02A/B17-460[»]
4KKCX-ray3.18A/B17-460[»]
4KKLX-ray2.85A/B17-460[»]
4LOUX-ray2.98A/B17-460[»]
4MQXX-ray3.52A/B1-465[»]
ProteinModelPortaliP37019.
SMRiP37019.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259368. 1 interactor.
DIPiDIP-9523N.
STRINGi511145.b0155.

Protein family/group databases

TCDBi2.A.49.5.1. the chloride carrier/channel (clc) family.

Proteomic databases

PaxDbiP37019.
PRIDEiP37019.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73266; AAC73266; b0155.
BAB96732; BAB96732; BAB96732.
GeneIDi946715.
KEGGiecj:JW5012.
eco:b0155.
PATRICi32115419. VBIEscCol129921_0161.

Organism-specific databases

EchoBASEiEB2235.
EcoGeneiEG12331. clcA.

Phylogenomic databases

eggNOGiENOG4105CMQ. Bacteria.
COG0038. LUCA.
HOGENOMiHOG000257546.
InParanoidiP37019.
KOiK03281.
OMAiFPAYHLD.
PhylomeDBiP37019.

Enzyme and pathway databases

BioCyciEcoCyc:YADQ-MONOMER.
ECOL316407:JW5012-MONOMER.
MetaCyc:YADQ-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP37019.
PROiP37019.

Family and domain databases

Gene3Di1.10.3080.10. 1 hit.
HAMAPiMF_01128. CLC_ClcA. 1 hit.
InterProiIPR023861. Cl-channel_ClcA.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
[Graphical view]
PfamiPF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
SUPFAMiSSF81340. SSF81340. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCLCA_ECOLI
AccessioniPrimary (citable) accession number: P37019
Secondary accession number(s): P77394
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: April 27, 2001
Last modified: November 2, 2016
This is version 160 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be a voltage-gated ClC-type chloride channel.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.