Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

H(+)/Cl(-) exchange transporter ClcA

Gene

clcA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme acid resistance (XAR) response.5 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei107 – 1071Chloride
Sitei148 – 1481Mediates proton transfer from the outer aqueous phase to the interior of the protein; involved in linking H(+) and Cl(-) transport
Sitei203 – 2031Mediates proton transfer from the protein to the inner aqueous phase
Binding sitei356 – 3561Chloride; via amide nitrogen
Binding sitei357 – 3571Chloride; via amide nitrogen
Binding sitei445 – 4451Chloride

GO - Molecular functioni

  • anion binding Source: EcoCyc
  • chloride ion binding Source: EcoCyc
  • solute:proton antiporter activity Source: EcoCyc
  • voltage-gated chloride channel activity Source: GO_Central

GO - Biological processi

  • chloride transmembrane transport Source: GO_Central
  • chloride transport Source: EcoCyc
  • nitrate transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Antiport, Ion transport, Stress response, Transport

Keywords - Ligandi

Chloride

Enzyme and pathway databases

BioCyciEcoCyc:YADQ-MONOMER.
ECOL316407:JW5012-MONOMER.
MetaCyc:YADQ-MONOMER.

Protein family/group databases

TCDBi2.A.49.5.1. the chloride carrier/channel (clc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
H(+)/Cl(-) exchange transporter ClcA
Alternative name(s):
ClC-ec1
Gene namesi
Name:clcA
Synonyms:eriC, yadQ
Ordered Locus Names:b0155, JW5012
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12331. clcA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3232CytoplasmicAdd
BLAST
Transmembranei33 – 6937HelicalAdd
BLAST
Topological domaini70 – 767Periplasmic
Transmembranei77 – 10024HelicalAdd
BLAST
Intramembranei109 – 1168Helical
Topological domaini117 – 1237Cytoplasmic
Transmembranei124 – 14118HelicalAdd
BLAST
Transmembranei148 – 16619HelicalAdd
BLAST
Topological domaini167 – 17610Cytoplasmic
Intramembranei177 – 18913HelicalAdd
BLAST
Intramembranei193 – 2019Helical
Topological domaini202 – 21413CytoplasmicAdd
BLAST
Transmembranei215 – 23218HelicalAdd
BLAST
Topological domaini233 – 25220PeriplasmicAdd
BLAST
Transmembranei253 – 28129HelicalAdd
BLAST
Topological domaini282 – 2876Cytoplasmic
Transmembranei288 – 30922HelicalAdd
BLAST
Topological domaini310 – 32920PeriplasmicAdd
BLAST
Transmembranei330 – 34920HelicalAdd
BLAST
Transmembranei355 – 37622HelicalAdd
BLAST
Topological domaini377 – 38610Periplasmic
Intramembranei387 – 40115HelicalAdd
BLAST
Intramembranei402 – 4043Note=Loop between two helices
Intramembranei405 – 41612HelicalAdd
BLAST
Intramembranei417 – 4215Note=Loop between two helices
Transmembranei422 – 43817HelicalAdd
BLAST
Topological domaini439 – 47335CytoplasmicAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi107 – 1071S → A: Uncouples chloride transport from proton transport. 1 Publication
Mutagenesisi148 – 1481E → A or Q: Abolishes proton transport, but permits the transit of chloride ions. Abolishes gating, permitting continuous rapid transit of chloride ions; when associated with A-445. 4 Publications
Mutagenesisi203 – 2031E → A, G, Q, S or T: Abolishes proton transport, and reduces chloride transport. 1 Publication
Mutagenesisi203 – 2031E → C, I, L or V: Abolishes proton and chloride transport. 1 Publication
Mutagenesisi203 – 2031E → D or H: No effect on proton and chloride transport. 1 Publication
Mutagenesisi203 – 2031E → K or R: Decreased proton and chloride transport. 1 Publication
Mutagenesisi445 – 4451Y → A: Abolishes gating, permitting continuous rapid transit of chloride ions; when associated with A-148. 3 Publications
Mutagenesisi445 – 4451Y → F or W: No effect. 3 Publications
Mutagenesisi445 – 4451Y → L: Alters stoichiometry of proton/chloride exchange. 3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 473473H(+)/Cl(-) exchange transporter ClcAPRO_0000094473Add
BLAST

Proteomic databases

PaxDbiP37019.

Expressioni

Inductioni

By acid-shock conditions.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4259368. 1 interaction.
DIPiDIP-9523N.
STRINGi511145.b0155.

Structurei

Secondary structure

1
473
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi18 – 269Combined sources
Turni27 – 293Combined sources
Helixi33 – 7038Combined sources
Beta strandi72 – 743Combined sources
Helixi75 – 10026Combined sources
Helixi102 – 1043Combined sources
Helixi109 – 1168Combined sources
Helixi124 – 14017Combined sources
Helixi148 – 16518Combined sources
Helixi171 – 19020Combined sources
Helixi193 – 20210Combined sources
Beta strandi203 – 2053Combined sources
Beta strandi207 – 2093Combined sources
Helixi215 – 23117Combined sources
Turni232 – 2354Combined sources
Helixi249 – 2513Combined sources
Helixi252 – 28433Combined sources
Helixi288 – 30821Combined sources
Helixi310 – 3123Combined sources
Beta strandi313 – 3164Combined sources
Helixi319 – 3246Combined sources
Helixi330 – 34819Combined sources
Turni349 – 3513Combined sources
Beta strandi353 – 3564Combined sources
Helixi357 – 37822Combined sources
Helixi380 – 3823Combined sources
Helixi386 – 3949Combined sources
Helixi396 – 4016Combined sources
Helixi405 – 41612Combined sources
Helixi419 – 4213Combined sources
Helixi422 – 43817Combined sources
Helixi444 – 45815Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KPKX-ray3.50A/B/C/D/E/F1-473[»]
1OTSX-ray2.51A/B1-465[»]
1OTTX-ray3.00A/B1-465[»]
1OTUX-ray3.30A/B1-465[»]
2EXWX-ray3.20A/B1-473[»]
2EXYX-ray3.10A/B1-473[»]
2EZ0X-ray3.54A/B1-473[»]
2FECX-ray3.97A/B1-465[»]
2FEDX-ray3.32A/B1-465[»]
2FEEX-ray3.20A/B1-465[»]
2H2PX-ray3.10A/B1-465[»]
2H2SX-ray3.10A/B1-465[»]
2HLFX-ray3.30A/B17-460[»]
2HT2X-ray3.32A/B1-473[»]
2HT3X-ray3.30A/B1-473[»]
2HT4X-ray3.20A/B1-473[»]
2HTKX-ray3.41A/B1-473[»]
2HTLX-ray3.40A/B1-473[»]
2R9HX-ray3.10A/B17-460[»]
3DETX-ray2.80A/B1-473[»]
3EJYX-ray3.20A/B1-473[»]
3EJZX-ray2.90A/B1-473[»]
3NMOX-ray3.10A1-465[»]
4ENEX-ray2.40A/B17-460[»]
4FTPX-ray3.21A1-465[»]
4KJPX-ray3.20A/B17-460[»]
4KJQX-ray2.88A/B17-460[»]
4KJWX-ray3.03A/B17-460[»]
4KK5X-ray3.17A/B17-460[»]
4KK6X-ray3.18A/B17-460[»]
4KK8X-ray2.86A/B17-460[»]
4KK9X-ray3.00A/B17-460[»]
4KKAX-ray3.00A/B17-460[»]
4KKBX-ray3.02A/B17-460[»]
4KKCX-ray3.18A/B17-460[»]
4KKLX-ray2.85A/B17-460[»]
4LOUX-ray2.98A/B17-460[»]
4MQXX-ray3.52A/B1-465[»]
ProteinModelPortaliP37019.
SMRiP37019. Positions 17-460.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37019.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi106 – 1105Selectivity filter part_1
Motifi146 – 1505Selectivity filter part_2
Motifi355 – 3595Selectivity filter part_3

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CMQ. Bacteria.
COG0038. LUCA.
HOGENOMiHOG000257546.
InParanoidiP37019.
KOiK03281.
OMAiFPAYHLD.
PhylomeDBiP37019.

Family and domain databases

Gene3Di1.10.3080.10. 1 hit.
HAMAPiMF_01128. CLC_ClcA. 1 hit.
InterProiIPR023861. Cl-channel_ClcA.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
[Graphical view]
PfamiPF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
SUPFAMiSSF81340. SSF81340. 1 hit.

Sequencei

Sequence statusi: Complete.

P37019-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTDTPSLET PQAARLRRRQ LIRQLLERDK TPLAILFMAA VVGTLVGLAA
60 70 80 90 100
VAFDKGVAWL QNQRMGALVH TADNYPLLLT VAFLCSAVLA MFGYFLVRKY
110 120 130 140 150
APEAGGSGIP EIEGALEDQR PVRWWRVLPV KFFGGLGTLG GGMVLGREGP
160 170 180 190 200
TVQIGGNIGR MVLDIFRLKG DEARHTLLAT GAAAGLAAAF NAPLAGILFI
210 220 230 240 250
IEEMRPQFRY TLISIKAVFI GVIMSTIMYR IFNHEVALID VGKLSDAPLN
260 270 280 290 300
TLWLYLILGI IFGIFGPIFN KWVLGMQDLL HRVHGGNITK WVLMGGAIGG
310 320 330 340 350
LCGLLGFVAP ATSGGGFNLI PIATAGNFSM GMLVFIFVAR VITTLLCFSS
360 370 380 390 400
GAPGGIFAPM LALGTVLGTA FGMVAVELFP QYHLEAGTFA IAGMGALLAA
410 420 430 440 450
SIRAPLTGII LVLEMTDNYQ LILPMIITGL GATLLAQFTG GKPLYSAILA
460 470
RTLAKQEAEQ LARSKAASAS ENT
Length:473
Mass (Da):50,349
Last modified:April 27, 2001 - v2
Checksum:i0B1BC39B417E9690
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti32 – 321P → Q (PubMed:8202364).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70214 Genomic DNA. Translation: AAB08585.1.
U00096 Genomic DNA. Translation: AAC73266.1.
AP009048 Genomic DNA. Translation: BAB96732.2.
PIRiC64739.
RefSeqiNP_414697.1. NC_000913.3.
WP_000845394.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73266; AAC73266; b0155.
BAB96732; BAB96732; BAB96732.
GeneIDi946715.
KEGGiecj:JW5012.
eco:b0155.
PATRICi32115419. VBIEscCol129921_0161.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70214 Genomic DNA. Translation: AAB08585.1.
U00096 Genomic DNA. Translation: AAC73266.1.
AP009048 Genomic DNA. Translation: BAB96732.2.
PIRiC64739.
RefSeqiNP_414697.1. NC_000913.3.
WP_000845394.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KPKX-ray3.50A/B/C/D/E/F1-473[»]
1OTSX-ray2.51A/B1-465[»]
1OTTX-ray3.00A/B1-465[»]
1OTUX-ray3.30A/B1-465[»]
2EXWX-ray3.20A/B1-473[»]
2EXYX-ray3.10A/B1-473[»]
2EZ0X-ray3.54A/B1-473[»]
2FECX-ray3.97A/B1-465[»]
2FEDX-ray3.32A/B1-465[»]
2FEEX-ray3.20A/B1-465[»]
2H2PX-ray3.10A/B1-465[»]
2H2SX-ray3.10A/B1-465[»]
2HLFX-ray3.30A/B17-460[»]
2HT2X-ray3.32A/B1-473[»]
2HT3X-ray3.30A/B1-473[»]
2HT4X-ray3.20A/B1-473[»]
2HTKX-ray3.41A/B1-473[»]
2HTLX-ray3.40A/B1-473[»]
2R9HX-ray3.10A/B17-460[»]
3DETX-ray2.80A/B1-473[»]
3EJYX-ray3.20A/B1-473[»]
3EJZX-ray2.90A/B1-473[»]
3NMOX-ray3.10A1-465[»]
4ENEX-ray2.40A/B17-460[»]
4FTPX-ray3.21A1-465[»]
4KJPX-ray3.20A/B17-460[»]
4KJQX-ray2.88A/B17-460[»]
4KJWX-ray3.03A/B17-460[»]
4KK5X-ray3.17A/B17-460[»]
4KK6X-ray3.18A/B17-460[»]
4KK8X-ray2.86A/B17-460[»]
4KK9X-ray3.00A/B17-460[»]
4KKAX-ray3.00A/B17-460[»]
4KKBX-ray3.02A/B17-460[»]
4KKCX-ray3.18A/B17-460[»]
4KKLX-ray2.85A/B17-460[»]
4LOUX-ray2.98A/B17-460[»]
4MQXX-ray3.52A/B1-465[»]
ProteinModelPortaliP37019.
SMRiP37019. Positions 17-460.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259368. 1 interaction.
DIPiDIP-9523N.
STRINGi511145.b0155.

Protein family/group databases

TCDBi2.A.49.5.1. the chloride carrier/channel (clc) family.

Proteomic databases

PaxDbiP37019.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73266; AAC73266; b0155.
BAB96732; BAB96732; BAB96732.
GeneIDi946715.
KEGGiecj:JW5012.
eco:b0155.
PATRICi32115419. VBIEscCol129921_0161.

Organism-specific databases

EchoBASEiEB2235.
EcoGeneiEG12331. clcA.

Phylogenomic databases

eggNOGiENOG4105CMQ. Bacteria.
COG0038. LUCA.
HOGENOMiHOG000257546.
InParanoidiP37019.
KOiK03281.
OMAiFPAYHLD.
PhylomeDBiP37019.

Enzyme and pathway databases

BioCyciEcoCyc:YADQ-MONOMER.
ECOL316407:JW5012-MONOMER.
MetaCyc:YADQ-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP37019.
PROiP37019.

Family and domain databases

Gene3Di1.10.3080.10. 1 hit.
HAMAPiMF_01128. CLC_ClcA. 1 hit.
InterProiIPR023861. Cl-channel_ClcA.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
[Graphical view]
PfamiPF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
SUPFAMiSSF81340. SSF81340. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCLCA_ECOLI
AccessioniPrimary (citable) accession number: P37019
Secondary accession number(s): P77394
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: April 27, 2001
Last modified: September 7, 2016
This is version 158 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be a voltage-gated ClC-type chloride channel.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.