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Protein

Anaerobic nitric oxide reductase transcription regulator NorR

Gene

norR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for the expression of anaerobic nitric oxide (NO) reductase, acts as a transcriptional activator for at least the norVW operon. Activation also requires sigma-54. Not required for induction of the aerobic NO-detoxifying enzyme NO dioxygenase. Binds to the promoter region of norVW, to a consensus target sequence, GT-(N7)-AC, which is highly conserved among proteobacteria.2 Publications

Pathwayi: nitric oxide reduction

This protein is involved in the pathway nitric oxide reduction, which is part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the pathway nitric oxide reduction and in Nitrogen metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi215 – 2228ATPSequence analysis
Nucleotide bindingi278 – 28710ATPSequence analysis
DNA bindingi479 – 49820H-T-H motifBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • phosphorelay signal transduction system Source: UniProtKB-HAMAP
  • positive regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12108-MONOMER.
ECOL316407:JW5843-MONOMER.
UniPathwayiUPA00638.

Names & Taxonomyi

Protein namesi
Recommended name:
Anaerobic nitric oxide reductase transcription regulator NorR
Gene namesi
Name:norR
Synonyms:ygaA
Ordered Locus Names:b2709, JW5843
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12108. norR.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 504504Anaerobic nitric oxide reductase transcription regulator NorRPRO_0000081153Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei57 – 5714-aspartylphosphateBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP37013.

Expressioni

Inductioni

By anaerobic conditions, however not induced by NO alone.

Interactioni

Protein-protein interaction databases

BioGridi4262260. 2 interactions.
DIPiDIP-12092N.
STRINGi511145.b2709.

Structurei

3D structure databases

ProteinModelPortaliP37013.
SMRiP37013. Positions 185-470.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini187 – 416230Sigma-54 factor interactionAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 186186NO sensor or transducerCuratedAdd
BLAST

Domaini

Deletion of amino acids 30-164 allows induction of NO reductase activity even in the absence of NO, i.e. the need for an NO-induced signal has been bypassed.

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108JUA. Bacteria.
COG3604. LUCA.
HOGENOMiHOG000058487.
InParanoidiP37013.
KOiK12266.
OMAiLRYEAHQ.
OrthoDBiEOG6WHNMG.
PhylomeDBiP37013.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.30.450.40. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01314. NorR.
InterProiIPR003593. AAA+_ATPase.
IPR003018. GAF.
IPR029016. GAF_dom-like.
IPR009057. Homeodomain-like.
IPR023944. NorR.
IPR027417. P-loop_NTPase.
IPR002078. Sigma_54_int.
IPR025662. Sigma_54_int_dom_ATP-bd_1.
IPR025943. Sigma_54_int_dom_ATP-bd_2.
IPR025944. Sigma_54_int_dom_CS.
[Graphical view]
PfamiPF01590. GAF. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00065. GAF. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF55781. SSF55781. 1 hit.
PROSITEiPS00675. SIGMA54_INTERACT_1. 1 hit.
PS00676. SIGMA54_INTERACT_2. 1 hit.
PS00688. SIGMA54_INTERACT_3. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37013-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFSVDVLAN IAIELQRGIG HQDRFQRLIT TLRQVLECDA SALLRYDSRQ
60 70 80 90 100
FIPLAIDGLA KDVLGRRFAL EGHPRLEAIA RAGDVVRFPA DSELPDPYDG
110 120 130 140 150
LIPGQESLKV HACVGLPLFA GQNLIGALTL DGMQPDQFDV FSDEELRLIA
160 170 180 190 200
ALAAGALSNA LLIEQLESQN MLPGDATPFE AVKQTQMIGL SPGMTQLKKE
210 220 230 240 250
IEIVAASDLN VLISGETGTG KELVAKAIHE ASPRAVNPLV YLNCAALPES
260 270 280 290 300
VAESELFGHV KGAFTGAISN RSGKFEMADN GTLFLDEIGE LSLALQAKLL
310 320 330 340 350
RVLQYGDIQR VGDDRCLRVD VRVLAATNRD LREEVLAGRF RADLFHRLSV
360 370 380 390 400
FPLSVPPLRE RGDDVILLAG YFCEQCRLRQ GLSRVVLSAG ARNLLQHYSF
410 420 430 440 450
PGNVRELEHA IHRAVVLARA TRSGDEVILE AQHFAFPEVT LPTPEVAAVP
460 470 480 490 500
VVKQNLREAT EAFQRETIRQ ALAQNHHNWA ACARMLETDV ANLHRLAKRL

GLKD
Length:504
Mass (Da):55,236
Last modified:March 25, 2003 - v2
Checksum:iB868CF41494DFCC3
GO

Sequence cautioni

The sequence AAA69218.1 differs from that shown. Reason: Frameshift at position 324. Curated
The sequence AAA69219.1 differs from that shown. Reason: Frameshift at position 324. Curated
The sequence AAA92665.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03846 Genomic DNA. Translation: AAA92665.1. Different initiation.
U03846 Genomic DNA. Translation: AAA92666.1.
D28595 Genomic DNA. Translation: BAA05931.1.
U29579 Genomic DNA. Translation: AAA69219.1. Frameshift.
U29579 Genomic DNA. Translation: AAA69218.1. Frameshift.
U00096 Genomic DNA. Translation: AAC75751.2.
AP009048 Genomic DNA. Translation: BAE76786.1.
PIRiA65051.
RefSeqiNP_417189.2. NC_000913.3.
WP_000010749.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75751; AAC75751; b2709.
BAE76786; BAE76786; BAE76786.
GeneIDi947186.
KEGGiecj:JW5843.
eco:b2709.
PATRICi32120816. VBIEscCol129921_2800.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03846 Genomic DNA. Translation: AAA92665.1. Different initiation.
U03846 Genomic DNA. Translation: AAA92666.1.
D28595 Genomic DNA. Translation: BAA05931.1.
U29579 Genomic DNA. Translation: AAA69219.1. Frameshift.
U29579 Genomic DNA. Translation: AAA69218.1. Frameshift.
U00096 Genomic DNA. Translation: AAC75751.2.
AP009048 Genomic DNA. Translation: BAE76786.1.
PIRiA65051.
RefSeqiNP_417189.2. NC_000913.3.
WP_000010749.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP37013.
SMRiP37013. Positions 185-470.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262260. 2 interactions.
DIPiDIP-12092N.
STRINGi511145.b2709.

Proteomic databases

PaxDbiP37013.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75751; AAC75751; b2709.
BAE76786; BAE76786; BAE76786.
GeneIDi947186.
KEGGiecj:JW5843.
eco:b2709.
PATRICi32120816. VBIEscCol129921_2800.

Organism-specific databases

EchoBASEiEB2032.
EcoGeneiEG12108. norR.

Phylogenomic databases

eggNOGiENOG4108JUA. Bacteria.
COG3604. LUCA.
HOGENOMiHOG000058487.
InParanoidiP37013.
KOiK12266.
OMAiLRYEAHQ.
OrthoDBiEOG6WHNMG.
PhylomeDBiP37013.

Enzyme and pathway databases

UniPathwayiUPA00638.
BioCyciEcoCyc:EG12108-MONOMER.
ECOL316407:JW5843-MONOMER.

Miscellaneous databases

PROiP37013.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.30.450.40. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01314. NorR.
InterProiIPR003593. AAA+_ATPase.
IPR003018. GAF.
IPR029016. GAF_dom-like.
IPR009057. Homeodomain-like.
IPR023944. NorR.
IPR027417. P-loop_NTPase.
IPR002078. Sigma_54_int.
IPR025662. Sigma_54_int_dom_ATP-bd_1.
IPR025943. Sigma_54_int_dom_ATP-bd_2.
IPR025944. Sigma_54_int_dom_CS.
[Graphical view]
PfamiPF01590. GAF. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00065. GAF. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF55781. SSF55781. 1 hit.
PROSITEiPS00675. SIGMA54_INTERACT_1. 1 hit.
PS00676. SIGMA54_INTERACT_2. 1 hit.
PS00688. SIGMA54_INTERACT_3. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "DNA sequence of a gene in Escherichia coli encoding a putative tripartite transcription factor with receiver, ATPase and DNA binding domains."
    Ramseier T.M., Figge R.M., Saier M.H. Jr.
    DNA Seq. 5:17-24(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Sequencing and characterization of the downstream region of hydA in Escherichia coli."
    Yano K., Ikebukuro K., Takada Y., Tomiyama M., Karube I.
    Submitted (FEB-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Flavorubredoxin, an inducible catalyst for nitric oxide reduction and detoxification in Escherichia coli."
    Gardner A.M., Helmick R.A., Gardner P.R.
    J. Biol. Chem. 277:8172-8177(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: ROLE IN NORV AND NORW EXPRESSION.
    Strain: K12 / AB1157.
  6. "Regulation of the flavorubredoxin nitric oxide reductase gene in Escherichia coli: nitrate repression, nitrite induction, and possible post-transcription control."
    da Costa P.N., Teixeira M., Saraiva L.M.
    FEMS Microbiol. Lett. 218:385-393(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: ROLE IN NORV AND NORW EXPRESSION.
    Strain: K12.
  7. "Regulation of the nitric oxide reduction operon (norRVW) in Escherichia coli: role of NorR and sigma 54 in the nitric oxide stress response."
    Gardner A.M., Gessner C.R., Gardner P.R.
    J. Biol. Chem. 278:10081-10086(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, REQUIREMENT OF SIGMA 54 FOR TRANSCRIPTIONAL ACTIVATION.
    Strain: K12 / AB1157.
  8. "DNA binding activity of the Escherichia coli nitric oxide sensor NorR suggests a conserved target sequence in diverse proteobacteria."
    Tucker N.P., D'Autreaux B., Studholme D.J., Spiro S., Dixon R.
    J. Bacteriol. 186:6656-6660(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: DNA-BINDING.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.

Entry informationi

Entry nameiNORR_ECOLI
AccessioniPrimary (citable) accession number: P37013
Secondary accession number(s): Q2MAC0, Q46875, Q46876
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: March 25, 2003
Last modified: February 17, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.