P37013 (NORR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anaerobic nitric oxide reductase transcription regulator norR

Gene names

Name:	norR
Synonyms:	ygaA
Ordered Locus Names:	b2709, JW5843

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the expression of anaerobic nitric oxide (NO) reductase, acts as a transcriptional activator for at least the norVW operon. Activation also requires sigma-54. Not required for induction of the aerobic NO-detoxifying enzyme NO dioxygenase. Binds to the promoter region of norVW, to a consensus target sequence, GT-(N7)-AC, which is highly conserved among proteobacteria. Ref.5 Ref.6
Pathway	Nitrogen metabolism; nitric oxide reduction. HAMAP MF_01314
Induction	By anaerobic conditions, however not induced by NO alone. HAMAP MF_01314
Domain	Deletion of amino acids 30-164 allows induction of NO reductase activity even in the absence of NO, i.e., the need for an NO-induced signal has been bypassed. HAMAP MF_01314
Sequence similarities	Contains 1 sigma-54 factor interaction domain.
Sequence caution	The sequence AAA69218.1 differs from that shown. Reason: Frameshift at position 324. The sequence AAA69219.1 differs from that shown. Reason: Frameshift at position 324. The sequence AAA92665.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	ATP-binding DNA-binding Nucleotide-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro transcription factor binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 504	504	Anaerobic nitric oxide reductase transcription regulator norR HAMAP MF_01314		PRO_0000081153
Regions
	Domain	187 – 416	230	Sigma-54 factor interaction
	Nucleotide binding	215 – 222	8	ATP Potential
	Nucleotide binding	278 – 287	10	ATP Potential
	DNA binding	479 – 498	20	H-T-H motif By similarity
	Region	1 – 186	186	NO sensor or transducer Probable
Amino acid modifications
	Modified residue	57	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P37013 [UniParc]. Last modified March 25, 2003. Version 2. Checksum: B868CF41494DFCC3	FASTA	504	55,236
	10 20 30 40 50 60 MSFSVDVLAN IAIELQRGIG HQDRFQRLIT TLRQVLECDA SALLRYDSRQ FIPLAIDGLA 70 80 90 100 110 120 KDVLGRRFAL EGHPRLEAIA RAGDVVRFPA DSELPDPYDG LIPGQESLKV HACVGLPLFA 130 140 150 160 170 180 GQNLIGALTL DGMQPDQFDV FSDEELRLIA ALAAGALSNA LLIEQLESQN MLPGDATPFE 190 200 210 220 230 240 AVKQTQMIGL SPGMTQLKKE IEIVAASDLN VLISGETGTG KELVAKAIHE ASPRAVNPLV 250 260 270 280 290 300 YLNCAALPES VAESELFGHV KGAFTGAISN RSGKFEMADN GTLFLDEIGE LSLALQAKLL 310 320 330 340 350 360 RVLQYGDIQR VGDDRCLRVD VRVLAATNRD LREEVLAGRF RADLFHRLSV FPLSVPPLRE 370 380 390 400 410 420 RGDDVILLAG YFCEQCRLRQ GLSRVVLSAG ARNLLQHYSF PGNVRELEHA IHRAVVLARA 430 440 450 460 470 480 TRSGDEVILE AQHFAFPEVT LPTPEVAAVP VVKQNLREAT EAFQRETIRQ ALAQNHHNWA 490 500 ACARMLETDV ANLHRLAKRL GLKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence of a gene in Escherichia coli encoding a putative tripartite transcription factor with receiver, ATPase and DNA binding domains." Ramseier T.M., Figge R.M., Saier M.H. Jr. DNA Seq. 5:17-24(1994) [PubMed: 7894055] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Sequencing and characterization of the downstream region of hydA in Escherichia coli." Yano K., Ikebukuro K., Takada Y., Tomiyama M., Karube I. Submitted (FEB-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Flavorubredoxin, an inducible catalyst for nitric oxide reduction and detoxification in Escherichia coli." Gardner A.M., Helmick R.A., Gardner P.R. J. Biol. Chem. 277:8172-8177(2002) [PubMed: 11751865] [Abstract] Cited for: ROLE IN NORV AND NORW EXPRESSION. Strain: K12 / AB1157.
[6]	"Regulation of the flavorubredoxin nitric oxide reductase gene in Escherichia coli: nitrate repression, nitrite induction, and possible post-transcription control." da Costa P.N., Teixeira M., Saraiva L.M. FEMS Microbiol. Lett. 218:385-393(2003) [PubMed: 12586421] [Abstract] Cited for: ROLE IN NORV AND NORW EXPRESSION. Strain: K12.
[7]	"Regulation of the nitric oxide reduction operon (norRVW) in Escherichia coli: role of NorR and sigma 54 in the nitric oxide stress response." Gardner A.M., Gessner C.R., Gardner P.R. J. Biol. Chem. 278:10081-10086(2003) [PubMed: 12529359] [Abstract] Cited for: CHARACTERIZATION, REQUIREMENT OF SIGMA 54 FOR TRANSCRIPTIONAL ACTIVATION. Strain: K12 / AB1157.
[8]	"DNA binding activity of the Escherichia coli nitric oxide sensor NorR suggests a conserved target sequence in diverse proteobacteria." Tucker N.P., D'Autreaux B., Studholme D.J., Spiro S., Dixon R. J. Bacteriol. 186:6656-6660(2004) [PubMed: 15375149] [Abstract] Cited for: DNA-BINDING. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U03846 Genomic DNA. Translation: AAA92665.1. Different initiation. U03846 Genomic DNA. Translation: AAA92666.1. D28595 Genomic DNA. Translation: BAA05931.1. U29579 Genomic DNA. Translation: AAA69219.1. Frameshift. U29579 Genomic DNA. Translation: AAA69218.1. Frameshift. U00096 Genomic DNA. Translation: AAC75751.2. AP009048 Genomic DNA. Translation: BAE76786.1.
PIR	A65051.
RefSeq	NP_417189.2. NC_000913.2.
3D structure databases
ProteinModelPortal	P37013.
SMR	P37013. Positions 183-499.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-12092N.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000004661; EBESCP00000004661; EBESCG00000003803. EBESCT00000017631; EBESCP00000016922; EBESCG00000016687.
GeneID	947186.
GenomeReviews	Gene locus JW5843 in contig AP009048_GR. Gene locus b2709 in contig U00096_GR.
KEGG	ecj:JW5843. eco:b2709.
PATRIC	32120816. VBIEscCol129921_2800.
Organism-specific databases
EchoBASE	EB2032.
EcoGene	EG12108. norR.
Phylogenomic databases
eggNOG	COG3604.
GeneTree	EBGT00050000008657.
HOGENOM	HBG740884.
OMA	RATRNGD.
PhylomeDB	P37013.
ProtClustDB	PRK05022.
Enzyme and pathway databases
BioCyc	EcoCyc:EG12108-MONOMER.
Gene expression databases
Genevestigator	P37013.
Family and domain databases
HAMAP	MF_01314. NorR. [Tree]
InterPro	IPR003593. ATPase_AAA+_core. IPR003018. GAF. IPR009057. Homeodomain-like. IPR023944. NorR. IPR002078. RNA_pol_sigma_54_int. [Graphical view]
KO	K12266.
Pfam	PF01590. GAF. 1 hit. PF00158. Sigma54_activat. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. SM00065. GAF. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS00675. SIGMA54_INTERACT_1. 1 hit. PS00676. SIGMA54_INTERACT_2. 1 hit. PS00688. SIGMA54_INTERACT_3. 1 hit. PS50045. SIGMA54_INTERACT_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NORR_ECOLI

Accession

Primary (citable) accession number: P37013
Secondary accession number(s): Q2MAC0, Q46875, Q46876

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	March 25, 2003
Last modified:	January 25, 2012
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program