P37005 (LAST_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized tRNA/rRNA methyltransferase LasT
EC=2.1.1.-

Gene names

Name:	lasT
Synonyms:	yjtD
Ordered Locus Names:	b4403, JW4366

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Sequence similarities

Belongs to the RNA methyltransferase TrmH family.

Ontologies

Keywords
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA methylation Inferred from electronic annotation. Source: GOC RNA processing Inferred from electronic annotation. Source: InterPro
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro RNA methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 228	228	Uncharacterized tRNA/rRNA methyltransferase LasT		PRO_0000159775
Experimental info
	Sequence conflict	31	1	L → V in L20873. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P37005 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: 5B493EE3510820F7	FASTA	228	25,259
	10 20 30 40 50 60 MRITIILVAP ARAENIGAAA RAMKTMGFSD LRIVDSQAHL EPATRWVAHG SGDIIDNIKV 70 80 90 100 110 120 FPTLAESLHD VDFTVATTAR SRAKYHYYAT PVELVPLLEE KSSWMSHAAL VFGREDSGLT 130 140 150 160 170 180 NEELALADVL TGVPMVADYP SLNLGQAVMV YCYQLATLIQ QPAKSDATAD QHQLQALRER 190 200 210 220 AMTLLTTLAV ADDIKLVDWL QQRLGLLEQR DTAMLHRLLH DIEKNITK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Anaerobic activation of arcA transcription in Escherichia coli: roles of Fnr and ArcA." Compan I., Touati D. Mol. Microbiol. 11:955-964(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-175. Strain: K12.
[5]	Rudd K.E. Unpublished observations (MAR-1994) Cited for: IDENTIFICATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U14003 Genomic DNA. Translation: AAA97299.1. U00096 Genomic DNA. Translation: AAC77356.1. AP009048 Genomic DNA. Translation: BAE78392.1. L20873 Genomic DNA. No translation available.
PIR	S56627.
RefSeq	NP_418820.1. NC_000913.2. YP_492533.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P37005.
SMR	P37005. Positions 3-226.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-10084N.
IntAct	P37005. 9 interactions.
MINT	MINT-1241070.
STRING	511145.b4403.
Proteomic databases
PaxDb	P37005.
PRIDE	P37005.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC77356; AAC77356; b4403. BAE78392; BAE78392; BAE78392.
GeneID	12933796. 948924.
KEGG	ecj:Y75_p4287. eco:b4403.
PATRIC	32124422. VBIEscCol129921_4551.
Organism-specific databases
EchoBASE	EB2215.
EcoGene	EG12309. lasT.
Phylogenomic databases
eggNOG	COG0565.
HOGENOM	HOG000229629.
KO	K02533.
OMA	DKKPAMQ.
ProtClustDB	PRK10433.
Enzyme and pathway databases
BioCyc	EcoCyc:EG12309-MONOMER. ECOL316407:JW4366-MONOMER.
Gene expression databases
Genevestigator	P37005.
Family and domain databases
InterPro	IPR004384. rRNA_MeTrfase_TrmH_1. IPR001537. SpoU_MeTrfase. [Graphical view]
Pfam	PF00588. SpoU_methylase. 1 hit. [Graphical view]
PIRSF	PIRSF004808. LasT. 1 hit.
TIGRFAMs	TIGR00050. rRNA_methyl_1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LAST_ECOLI

Accession

Primary (citable) accession number: P37005
Secondary accession number(s): Q2M5R4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program