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Protein

Uncharacterized tRNA/rRNA methyltransferase LasT

Gene

lasT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei113S-adenosyl-L-methionine; via amide nitrogenBy similarity1
Binding sitei133S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG12309-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Uncharacterized tRNA/rRNA methyltransferase LasTCurated (EC:2.1.1.-)
Gene namesi
Name:lasT
Synonyms:yjtD
Ordered Locus Names:b4403, JW4366
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12309 lasT

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001597751 – 228Uncharacterized tRNA/rRNA methyltransferase LasTAdd BLAST228

Proteomic databases

PaxDbiP37005
PRIDEiP37005

Interactioni

Protein-protein interaction databases

BioGridi4262790, 19 interactors
DIPiDIP-10084N
IntActiP37005, 9 interactors
STRINGi316385.ECDH10B_4561

Structurei

3D structure databases

ProteinModelPortaliP37005
SMRiP37005
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni77 – 79S-adenosyl-L-methionine bindingBy similarity3
Regioni140 – 142S-adenosyl-L-methionine bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4106FVC Bacteria
COG0565 LUCA
HOGENOMiHOG000229629
InParanoidiP37005
KOiK02533
OMAiVYCYQLS
PhylomeDBiP37005

Family and domain databases

Gene3Di3.40.1280.10, 1 hit
InterProiView protein in InterPro
IPR029028 Alpha/beta_knot_MTases
IPR004384 rRNA_MeTrfase_TrmH_1
IPR001537 SpoU_MeTrfase
IPR029026 tRNA_m1G_MTases_N
PANTHERiPTHR42786 PTHR42786, 1 hit
PfamiView protein in Pfam
PF00588 SpoU_methylase, 1 hit
PIRSFiPIRSF004808 LasT, 1 hit
SUPFAMiSSF75217 SSF75217, 1 hit
TIGRFAMsiTIGR00050 rRNA_methyl_1, 1 hit

Sequencei

Sequence statusi: Complete.

P37005-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRITIILVAP ARAENIGAAA RAMKTMGFSD LRIVDSQAHL EPATRWVAHG
60 70 80 90 100
SGDIIDNIKV FPTLAESLHD VDFTVATTAR SRAKYHYYAT PVELVPLLEE
110 120 130 140 150
KSSWMSHAAL VFGREDSGLT NEELALADVL TGVPMVADYP SLNLGQAVMV
160 170 180 190 200
YCYQLATLIQ QPAKSDATAD QHQLQALRER AMTLLTTLAV ADDIKLVDWL
210 220
QQRLGLLEQR DTAMLHRLLH DIEKNITK
Length:228
Mass (Da):25,259
Last modified:February 1, 1995 - v2
Checksum:i5B493EE3510820F7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti31L → V in L20873 (PubMed:8022271).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA Translation: AAA97299.1
U00096 Genomic DNA Translation: AAC77356.1
AP009048 Genomic DNA Translation: BAE78392.1
L20873 Genomic DNA No translation available.
PIRiS56627
RefSeqiNP_418820.1, NC_000913.3
WP_001223132.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77356; AAC77356; b4403
BAE78392; BAE78392; BAE78392
GeneIDi948924
KEGGiecj:JW4366
eco:b4403
PATRICifig|1411691.4.peg.2282

Similar proteinsi

Entry informationi

Entry nameiLAST_ECOLI
AccessioniPrimary (citable) accession number: P37005
Secondary accession number(s): Q2M5R4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: February 1, 1995
Last modified: April 25, 2018
This is version 131 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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