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Protein

Uncharacterized tRNA/rRNA methyltransferase LasT

Gene

lasT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Enzyme and pathway databases

BioCyciEcoCyc:EG12309-MONOMER.
ECOL316407:JW4366-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Uncharacterized tRNA/rRNA methyltransferase LasT (EC:2.1.1.-)
Gene namesi
Name:lasT
Synonyms:yjtD
Ordered Locus Names:b4403, JW4366
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12309. lasT.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 228228Uncharacterized tRNA/rRNA methyltransferase LasTPRO_0000159775Add
BLAST

Proteomic databases

PaxDbiP37005.
PRIDEiP37005.

Interactioni

Protein-protein interaction databases

BioGridi4262790. 11 interactions.
DIPiDIP-10084N.
IntActiP37005. 9 interactions.
MINTiMINT-1241070.
STRINGi511145.b4403.

Structurei

3D structure databases

ProteinModelPortaliP37005.
SMRiP37005. Positions 3-226.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4106FVC. Bacteria.
COG0565. LUCA.
HOGENOMiHOG000229629.
InParanoidiP37005.
KOiK02533.
OMAiDGLKNED.
PhylomeDBiP37005.

Family and domain databases

Gene3Di3.40.1280.10. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR004384. rRNA_MeTrfase_TrmH_1.
IPR001537. SpoU_MeTrfase.
IPR029026. tRNA_m1G_MTases_N.
[Graphical view]
PfamiPF00588. SpoU_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF004808. LasT. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00050. rRNA_methyl_1. 1 hit.

Sequencei

Sequence statusi: Complete.

P37005-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRITIILVAP ARAENIGAAA RAMKTMGFSD LRIVDSQAHL EPATRWVAHG
60 70 80 90 100
SGDIIDNIKV FPTLAESLHD VDFTVATTAR SRAKYHYYAT PVELVPLLEE
110 120 130 140 150
KSSWMSHAAL VFGREDSGLT NEELALADVL TGVPMVADYP SLNLGQAVMV
160 170 180 190 200
YCYQLATLIQ QPAKSDATAD QHQLQALRER AMTLLTTLAV ADDIKLVDWL
210 220
QQRLGLLEQR DTAMLHRLLH DIEKNITK
Length:228
Mass (Da):25,259
Last modified:February 1, 1995 - v2
Checksum:i5B493EE3510820F7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti31 – 311L → V in L20873 (PubMed:8022271).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97299.1.
U00096 Genomic DNA. Translation: AAC77356.1.
AP009048 Genomic DNA. Translation: BAE78392.1.
L20873 Genomic DNA. No translation available.
PIRiS56627.
RefSeqiNP_418820.1. NC_000913.3.
WP_001223132.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77356; AAC77356; b4403.
BAE78392; BAE78392; BAE78392.
GeneIDi948924.
KEGGiecj:JW4366.
eco:b4403.
PATRICi32124422. VBIEscCol129921_4551.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97299.1.
U00096 Genomic DNA. Translation: AAC77356.1.
AP009048 Genomic DNA. Translation: BAE78392.1.
L20873 Genomic DNA. No translation available.
PIRiS56627.
RefSeqiNP_418820.1. NC_000913.3.
WP_001223132.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP37005.
SMRiP37005. Positions 3-226.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262790. 11 interactions.
DIPiDIP-10084N.
IntActiP37005. 9 interactions.
MINTiMINT-1241070.
STRINGi511145.b4403.

Proteomic databases

PaxDbiP37005.
PRIDEiP37005.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77356; AAC77356; b4403.
BAE78392; BAE78392; BAE78392.
GeneIDi948924.
KEGGiecj:JW4366.
eco:b4403.
PATRICi32124422. VBIEscCol129921_4551.

Organism-specific databases

EchoBASEiEB2215.
EcoGeneiEG12309. lasT.

Phylogenomic databases

eggNOGiENOG4106FVC. Bacteria.
COG0565. LUCA.
HOGENOMiHOG000229629.
InParanoidiP37005.
KOiK02533.
OMAiDGLKNED.
PhylomeDBiP37005.

Enzyme and pathway databases

BioCyciEcoCyc:EG12309-MONOMER.
ECOL316407:JW4366-MONOMER.

Miscellaneous databases

PROiP37005.

Family and domain databases

Gene3Di3.40.1280.10. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR004384. rRNA_MeTrfase_TrmH_1.
IPR001537. SpoU_MeTrfase.
IPR029026. tRNA_m1G_MTases_N.
[Graphical view]
PfamiPF00588. SpoU_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF004808. LasT. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00050. rRNA_methyl_1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLAST_ECOLI
AccessioniPrimary (citable) accession number: P37005
Secondary accession number(s): Q2M5R4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: February 1, 1995
Last modified: September 7, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.