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Protein

Putative fluoride ion transporter CrcB

Gene

crcB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Important for reducing fluoride concentration in the cell, thus reducing its toxicity.UniRule annotation1 Publication

GO - Molecular functioni

  • inorganic anion transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • inorganic anion transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG12209-MONOMER.
ECOL316407:JW0619-MONOMER.
MetaCyc:EG12209-MONOMER.

Protein family/group databases

TCDBi1.A.43.1.1. the camphor resistance (crcb) or fluoride exporter (fex) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative fluoride ion transporter CrcBUniRule annotation
Gene namesi
Name:crcBUniRule annotation
Synonyms:ybeI
Ordered Locus Names:b0624, JW0619
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12209. crcB.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei4 – 2421HelicalUniRule annotationAdd
BLAST
Transmembranei35 – 5521HelicalUniRule annotationAdd
BLAST
Transmembranei71 – 9121HelicalUniRule annotationAdd
BLAST
Transmembranei103 – 12321HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutants show increased sensitivity to fluoride (PubMed:22194412). Deletion of pagP (crcA), cspE and crcB increases sensitivity to camphor and exacerbates the nucleoid morphology defects of the topoisomerase IV temperature-sensitive mutants (PubMed:12904550).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 127127Putative fluoride ion transporter CrcBPRO_0000110097Add
BLAST

Proteomic databases

PaxDbiP37002.

Expressioni

Inductioni

By fluoride, via a fluoride-responsive riboswitch.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4260641. 196 interactions.
IntActiP37002. 1 interaction.
STRINGi511145.b0624.

Structurei

3D structure databases

ProteinModelPortaliP37002.
SMRiP37002. Positions 1-122.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CrcB (TC 9.B.71) family. [View classification]UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107Z6W. Bacteria.
COG0239. LUCA.
HOGENOMiHOG000052572.
InParanoidiP37002.
KOiK06199.
OMAiRFAITIG.
PhylomeDBiP37002.

Family and domain databases

HAMAPiMF_00454. CrcB. 1 hit.
InterProiIPR003691. CrcB.
[Graphical view]
PfamiPF02537. CRCB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00494. crcB. 1 hit.

Sequencei

Sequence statusi: Complete.

P37002-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLQLLLAVFI GGGTGSVARW LLSMRFNPLH QAIPLGTLTA NLIGAFIIGI
60 70 80 90 100
GFAWFSRMTN IDPVWKVLIT TGFCGGLTTF STFSAEVVFL LQEGRFGWAL
110 120
LNVFVNLLGS FAMTALAFWL FSASTAH
Length:127
Mass (Da):13,777
Last modified:June 1, 1994 - v1
Checksum:i5A0B2004D134BBF3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D28497 Genomic DNA. No translation available.
S83396 Genomic DNA. No translation available.
U82598 Genomic DNA. Translation: AAB40824.1.
U00096 Genomic DNA. Translation: AAC73725.1.
AP009048 Genomic DNA. Translation: BAA35267.1.
PIRiF64796.
RefSeqiNP_415157.1. NC_000913.3.
WP_000939747.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73725; AAC73725; b0624.
BAA35267; BAA35267; BAA35267.
GeneIDi945798.
KEGGiecj:JW0619.
eco:b0624.
PATRICi32116428. VBIEscCol129921_0654.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D28497 Genomic DNA. No translation available.
S83396 Genomic DNA. No translation available.
U82598 Genomic DNA. Translation: AAB40824.1.
U00096 Genomic DNA. Translation: AAC73725.1.
AP009048 Genomic DNA. Translation: BAA35267.1.
PIRiF64796.
RefSeqiNP_415157.1. NC_000913.3.
WP_000939747.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP37002.
SMRiP37002. Positions 1-122.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260641. 196 interactions.
IntActiP37002. 1 interaction.
STRINGi511145.b0624.

Protein family/group databases

TCDBi1.A.43.1.1. the camphor resistance (crcb) or fluoride exporter (fex) family.

Proteomic databases

PaxDbiP37002.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73725; AAC73725; b0624.
BAA35267; BAA35267; BAA35267.
GeneIDi945798.
KEGGiecj:JW0619.
eco:b0624.
PATRICi32116428. VBIEscCol129921_0654.

Organism-specific databases

EchoBASEiEB2123.
EcoGeneiEG12209. crcB.

Phylogenomic databases

eggNOGiENOG4107Z6W. Bacteria.
COG0239. LUCA.
HOGENOMiHOG000052572.
InParanoidiP37002.
KOiK06199.
OMAiRFAITIG.
PhylomeDBiP37002.

Enzyme and pathway databases

BioCyciEcoCyc:EG12209-MONOMER.
ECOL316407:JW0619-MONOMER.
MetaCyc:EG12209-MONOMER.

Miscellaneous databases

PROiP37002.

Family and domain databases

HAMAPiMF_00454. CrcB. 1 hit.
InterProiIPR003691. CrcB.
[Graphical view]
PfamiPF02537. CRCB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00494. crcB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCRCB_ECOLI
AccessioniPrimary (citable) accession number: P37002
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 7, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Overexpression of pagP (crcA), cspE and crcB leads to camphor resistance, chromosome condensation and suppression of growth defects of mukB mutants (PubMed:8844142). Overexpression also increases levels of plasmid supercoiling, results in increased resistance to nalidixic acid in gyrase and topoisomerase IV temperature-sensitive mutants and affects the regulation of several genes (PubMed:12904550).2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.