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Protein

23S rRNA (guanine(745)-N(1))-methyltransferase

Gene

rlmA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates the guanosine in position 745 of 23S rRNA.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + guanine(745) in 23S rRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(745) in 23S rRNA.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi5ZincCombined sources1 Publication1
Metal bindingi8ZincCombined sources1 Publication1
Metal bindingi21ZincCombined sources1 Publication1
Metal bindingi25ZincCombined sources1 Publication1
Binding sitei67S-adenosyl-L-methionine1 Publication1
Binding sitei183S-adenosyl-L-methionine1 Publication1

GO - Molecular functioni

  • 23S rRNA (guanine(745)-N(1))-methyltransferase activity Source: UniProtKB-EC
  • rRNA (guanine-N1-)-methyltransferase activity Source: EcoCyc
  • zinc ion binding Source: EcoCyc

GO - Biological processi

  • rRNA base methylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

Metal-binding, S-adenosyl-L-methionine, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:EG12207-MONOMER.
ECOL316407:JW1811-MONOMER.
MetaCyc:EG12207-MONOMER.
BRENDAi2.1.1.187. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
23S rRNA (guanine(745)-N(1))-methyltransferase (EC:2.1.1.187)
Alternative name(s):
23S rRNA m1G745 methyltransferase
Ribosomal RNA large subunit methyltransferase A
Gene namesi
Name:rlmA
Synonyms:rrmA, yebH
Ordered Locus Names:b1822, JW1811
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12207. rlmA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000974441 – 26923S rRNA (guanine(745)-N(1))-methyltransferaseAdd BLAST269

Proteomic databases

PaxDbiP36999.
PRIDEiP36999.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4260324. 2 interactors.
IntActiP36999. 18 interactors.
STRINGi511145.b1822.

Structurei

Secondary structure

1269
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni6 – 8Combined sources3
Beta strandi13 – 15Combined sources3
Beta strandi18 – 20Combined sources3
Beta strandi26 – 29Combined sources4
Beta strandi40 – 43Combined sources4
Beta strandi49 – 51Combined sources3
Helixi52 – 62Combined sources11
Turni63 – 67Combined sources5
Helixi68 – 81Combined sources14
Beta strandi88 – 92Combined sources5
Helixi100 – 105Combined sources6
Beta strandi110 – 117Combined sources8
Helixi119 – 128Combined sources10
Beta strandi132 – 136Combined sources5
Beta strandi149 – 156Combined sources8
Helixi161 – 167Combined sources7
Beta strandi168 – 179Combined sources12
Turni181 – 184Combined sources4
Helixi185 – 188Combined sources4
Turni189 – 191Combined sources3
Beta strandi208 – 223Combined sources16
Helixi224 – 232Combined sources9
Helixi237 – 239Combined sources3
Helixi242 – 250Combined sources9
Beta strandi252 – 267Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1P91X-ray2.80A/B1-269[»]
ProteinModelPortaliP36999.
SMRiP36999.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP36999.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni96 – 97S-adenosyl-L-methionine binding1 Publication2

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105DHI. Bacteria.
COG0500. LUCA.
HOGENOMiHOG000040121.
InParanoidiP36999.
KOiK00563.
OMAiVQHKHSK.
PhylomeDBiP36999.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025714. Methyltranfer_dom.
IPR016718. rRNA_m1G-MeTrfase_A_prd.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF13847. Methyltransf_31. 1 hit.
[Graphical view]
PIRSFiPIRSF018249. MyrA_prd. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

P36999-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFSCPLCHQ PLSREKNSYI CPQRHQFDMA KEGYVNLLPV QHKRSRDPGD
60 70 80 90 100
SAEMMQARRA FLDAGHYQPL RDAIVAQLRE RLDDKATAVL DIGCGEGYYT
110 120 130 140 150
HAFADALPEI TTFGLDVSKV AIKAAAKRYP QVTFCVASSH RLPFSDTSMD
160 170 180 190 200
AIIRIYAPCK AEELARVVKP GGWVITATPG PRHLMELKGL IYNEVHLHAP
210 220 230 240 250
HAEQLEGFTL QQSAELCYPM RLRGDEAVAL LQMTPFAWRA KPEVWQTLAA
260
KEVFDCQTDF NIHLWQRSY
Length:269
Mass (Da):30,419
Last modified:June 1, 1994 - v1
Checksum:iF4A154A6EC3FB6D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D28496 Genomic DNA. Translation: BAA05855.1.
U00096 Genomic DNA. Translation: AAC74892.1.
AP009048 Genomic DNA. Translation: BAA15633.1.
PIRiS49049.
RefSeqiNP_416336.1. NC_000913.3.
WP_000010115.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74892; AAC74892; b1822.
BAA15633; BAA15633; BAA15633.
GeneIDi946340.
KEGGiecj:JW1811.
eco:b1822.
PATRICi32118963. VBIEscCol129921_1899.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D28496 Genomic DNA. Translation: BAA05855.1.
U00096 Genomic DNA. Translation: AAC74892.1.
AP009048 Genomic DNA. Translation: BAA15633.1.
PIRiS49049.
RefSeqiNP_416336.1. NC_000913.3.
WP_000010115.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1P91X-ray2.80A/B1-269[»]
ProteinModelPortaliP36999.
SMRiP36999.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260324. 2 interactors.
IntActiP36999. 18 interactors.
STRINGi511145.b1822.

Proteomic databases

PaxDbiP36999.
PRIDEiP36999.

Protocols and materials databases

DNASUi946340.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74892; AAC74892; b1822.
BAA15633; BAA15633; BAA15633.
GeneIDi946340.
KEGGiecj:JW1811.
eco:b1822.
PATRICi32118963. VBIEscCol129921_1899.

Organism-specific databases

EchoBASEiEB2122.
EcoGeneiEG12207. rlmA.

Phylogenomic databases

eggNOGiENOG4105DHI. Bacteria.
COG0500. LUCA.
HOGENOMiHOG000040121.
InParanoidiP36999.
KOiK00563.
OMAiVQHKHSK.
PhylomeDBiP36999.

Enzyme and pathway databases

BioCyciEcoCyc:EG12207-MONOMER.
ECOL316407:JW1811-MONOMER.
MetaCyc:EG12207-MONOMER.
BRENDAi2.1.1.187. 2026.

Miscellaneous databases

EvolutionaryTraceiP36999.
PROiP36999.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025714. Methyltranfer_dom.
IPR016718. rRNA_m1G-MeTrfase_A_prd.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF13847. Methyltransf_31. 1 hit.
[Graphical view]
PIRSFiPIRSF018249. MyrA_prd. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRLMA_ECOLI
AccessioniPrimary (citable) accession number: P36999
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 2, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.