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P36993 (PPM1B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein phosphatase 1B

EC=3.1.3.16
Alternative name(s):
Protein phosphatase 2C isoform beta
Short name=PP2C-beta
Gene names
Name:Ppm1b
Synonyms:Pp2c2, Pppm1b
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length390 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Enzyme with a broad specificity. Dephosphorylates PRKAA1 and PRKAA2. Inhibits TBK1-mediated antiviral signaling by dephosphorylating it at 'Ser-172'. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB. Ref.6

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Subunit structure

Monomer. Interacts with PAK6 By similarity. Interacts with the phosphorylated form of IKBKB/IKKB By similarity.

Subcellular location

Cytoplasmcytosol. Membrane. Note: Weakly associates at the membrane and N-myristoylation mediates the membrane localization. Ref.5 Ref.6

Tissue specificity

Beta-1 is expressed ubiquitously; beta-2 is expressed exclusively in brain and heart; beta-4 is expressed exclusively in brain and intestine; beta-3 and beta-5 are expressed exclusively in testis and intestine.

Post-translational modification

Isgylation negatively regulates its activity By similarity.

N-myristoylation is essential for the recognition of its substrates for dephosphorylation.

Sequence similarities

Belongs to the PP2C family.

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Note: Isoforms only differ in their C-terminus.
Isoform Beta-1 (identifier: P36993-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Beta-2 (identifier: P36993-2)

The sequence of this isoform differs from the canonical sequence as follows:
     379-390: GAGDLEDSLVAL → VSLHLFPKYLK
Isoform Beta-3 (identifier: P36993-3)

The sequence of this isoform differs from the canonical sequence as follows:
     379-390: GAGDLEDSLVAL → FYQPSIAYSDNVFLL
Isoform Beta-4 (identifier: P36993-4)

The sequence of this isoform differs from the canonical sequence as follows:
     379-390: GAGDLEDSLVAL → MADLSTSICKPS
Isoform Beta-5 (identifier: P36993-5)

The sequence of this isoform differs from the canonical sequence as follows:
     388-390: VAL → FYQPSIAYSDNVFLL

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 390389Protein phosphatase 1B
PRO_0000057747

Sites

Metal binding601Manganese 1 By similarity
Metal binding601Manganese 2 By similarity
Metal binding611Manganese 1; via carbonyl oxygen By similarity
Metal binding2431Manganese 2 By similarity
Metal binding2861Manganese 2 By similarity

Amino acid modifications

Lipidation21N-myristoyl glycine Ref.6
Cross-link12Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15) By similarity

Natural variations

Alternative sequence379 – 39012GAGDL…SLVAL → VSLHLFPKYLK in isoform Beta-2.
VSP_005089
Alternative sequence379 – 39012GAGDL…SLVAL → FYQPSIAYSDNVFLL in isoform Beta-3.
VSP_005090
Alternative sequence379 – 39012GAGDL…SLVAL → MADLSTSICKPS in isoform Beta-4.
VSP_005091
Alternative sequence388 – 3903VAL → FYQPSIAYSDNVFLL in isoform Beta-5.
VSP_005092

Sequences

Sequence LengthMass (Da)Tools
Isoform Beta-1 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 255C97B4276189FD

FASTA39042,795
        10         20         30         40         50         60 
MGAFLDKPKT EKHNAHGAGN GLRYGLSSMQ GWRVEMEDAH TAVVGIPHGL DNWSFFAVYD 

        70         80         90        100        110        120 
GHAGSRVANY CSTHLLEHIT TNEDFRAADK SGSALEPSVE SVKTGIRTGF LKIDEYMRNF 

       130        140        150        160        170        180 
SDLRNGMDRS GSTAVGVMVS PTHMYFINCG DSRAVLCRNG QVCFSTQDHK PCNPVEKERI 

       190        200        210        220        230        240 
QNAGGSVMIQ RVNGSLAVSR ALGDYDYKCV DGKGPTEQLV SPEPEVYEIV RAEEDEFVVL 

       250        260        270        280        290        300 
ACDGIWDVMS NEELCEFVKS RLEVSDDLEN VCNWVVDTCL HKGSRDNMSV VLVCFSNAPK 

       310        320        330        340        350        360 
VSEEAVKRDS ELDKHLESRV EEIMQKSGEE GMPDLAHVMR ILSAENIPNL PPGGGLAGKR 

       370        380        390 
HVIEAVYSRL NPHKDNDGGA GDLEDSLVAL 

« Hide

Isoform Beta-2 [UniParc].

Checksum: 17938754FC4B2D79
Show »

FASTA38942,980
Isoform Beta-3 [UniParc].

Checksum: BA1DAED2E2CFBFFC
Show »

FASTA39343,413
Isoform Beta-4 [UniParc].

Checksum: FEFDF945009E835F
Show »

FASTA39042,888
Isoform Beta-5 [UniParc].

Checksum: 807B4E64B6715E18
Show »

FASTA40244,271

References

[1]"Molecular cloning of a novel isotype of Mg(2+)-dependent protein phosphatase beta (type 2C beta) enriched in brain and heart."
Terasawa T., Kobayashi T., Murakami T., Ohnishi M., Kato S., Tanaka O., Kondo H., Yamamoto H., Takeuchi T., Tamura S.
Arch. Biochem. Biophys. 307:342-349(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Molecular cloning and expression of mouse Mg(2+)-dependent protein phosphatase beta-4 (type 2C beta-4)."
Kato S., Terasawa T., Kobayashi T., Ohnishi M., Sasahara Y., Kusuda K., Yanagawa Y., Hiraga A., Matsui Y., Tamura S.
Arch. Biochem. Biophys. 318:387-393(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (BETA-3; BETA-4 AND BETA-5).
Tissue: Testis.
[3]"Molecular cloning and expression of cDNAs encoding two isoforms of protein phosphatase 2C beta from mouse testis."
Hou E.W., Kawai Y., Miyasaka H., Li S.S.
Biochem. Mol. Biol. Int. 32:773-780(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (BETA-3 AND BETA-4).
Tissue: Testis.
[4]"Alternative promoters direct tissue-specific expression of the mouse protein phosphatase 2Cbeta gene."
Ohnishi M., Chida N., Kobayashi T., Wang H., Ikeda S., Hanada M., Yanagawa Y., Katsura K., Hiraga A., Tamura S.
Eur. J. Biochem. 263:736-745(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/Sv.
[5]"Protein phosphatase 5 modulates SMAD3 function in the transforming growth factor-? pathway."
Bruce D.L., Macartney T., Yong W., Shou W., Sapkota G.P.
Cell. Signal. 24:1999-2006(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[6]"N-Myristoylation is essential for protein phosphatases PPM1A and PPM1B to dephosphorylate their physiological substrates in cells."
Chida T., Ando M., Matsuki T., Masu Y., Nagaura Y., Takano-Yamamoto T., Tamura S., Kobayashi T.
Biochem. J. 449:741-749(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, MYRISTOYLATION AT GLY-2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D17411 mRNA. Translation: BAA04233.1.
D17412 mRNA. Translation: BAA04234.1.
D45859 mRNA. Translation: BAA08293.1.
D45860 mRNA. Translation: BAA08294.1.
D45861 mRNA. Translation: BAA08295.1.
U09218 mRNA. Translation: AAB60442.1.
AB007798 Genomic DNA. Translation: BAA84471.1.
PIRI49016.
S65672.
RefSeqNP_001152969.1. NM_001159497.1.
NP_001152970.1. NM_001159498.1.
NP_035281.1. NM_011151.2.
XP_006523924.1. XM_006523861.1.
XP_006523925.1. XM_006523862.1.
UniGeneMm.249695.

3D structure databases

ProteinModelPortalP36993.
SMRP36993. Positions 2-373.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP36993. 1 interaction.
MINTMINT-4108365.

PTM databases

PhosphoSiteP36993.

Proteomic databases

PaxDbP36993.
PRIDEP36993.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000112304; ENSMUSP00000107923; ENSMUSG00000061130. [P36993-1]
ENSMUST00000112305; ENSMUSP00000107924; ENSMUSG00000061130. [P36993-4]
ENSMUST00000112307; ENSMUSP00000107926; ENSMUSG00000061130. [P36993-3]
GeneID19043.
KEGGmmu:19043.
UCSCuc008dtf.2. mouse. [P36993-2]
uc008dth.2. mouse. [P36993-1]
uc008dti.2. mouse. [P36993-4]
uc008dtj.2. mouse. [P36993-3]
uc008dtk.2. mouse. [P36993-5]

Organism-specific databases

CTD5495.
MGIMGI:101841. Ppm1b.

Phylogenomic databases

eggNOGCOG0631.
GeneTreeENSGT00740000115384.
HOGENOMHOG000233895.
HOVERGENHBG053647.
KOK04461.
OrthoDBEOG7WMCJH.
PhylomeDBP36993.

Gene expression databases

ArrayExpressP36993.
BgeeP36993.
GenevestigatorP36993.

Family and domain databases

Gene3D1.10.10.430. 1 hit.
3.60.40.10. 1 hit.
InterProIPR001932. PP2C-like_dom.
IPR012911. PP2C_C.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERPTHR13832. PTHR13832. 1 hit.
PfamPF00481. PP2C. 1 hit.
PF07830. PP2C_C. 1 hit.
[Graphical view]
SMARTSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81601. SSF81601. 1 hit.
SSF81606. SSF81606. 1 hit.
PROSITEPS01032. PP2C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio295497.
PROP36993.
SOURCESearch...

Entry information

Entry namePPM1B_MOUSE
AccessionPrimary (citable) accession number: P36993
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 16, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot