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P36993

- PPM1B_MOUSE

UniProt

P36993 - PPM1B_MOUSE

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Protein

Protein phosphatase 1B

Gene

Ppm1b

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Enzyme with a broad specificity. Dephosphorylates PRKAA1 and PRKAA2. Inhibits TBK1-mediated antiviral signaling by dephosphorylating it at 'Ser-172'. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi60 – 601Manganese 1By similarity
Metal bindingi60 – 601Manganese 2By similarity
Metal bindingi61 – 611Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi243 – 2431Manganese 2By similarity
Metal bindingi286 – 2861Manganese 2By similarity

GO - Molecular functioni

  1. magnesium ion binding Source: InterPro
  2. manganese ion binding Source: InterPro
  3. protein serine/threonine phosphatase activity Source: InterPro

GO - Biological processi

  1. negative regulation of defense response to virus Source: UniProtKB
  2. negative regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
  3. negative regulation of interferon-beta production Source: UniProtKB
  4. negative regulation of NF-kappaB import into nucleus Source: UniProtKB
  5. N-terminal protein myristoylation Source: UniProtKB
  6. protein dephosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_198533. ISG15 antiviral mechanism.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1B (EC:3.1.3.16)
Alternative name(s):
Protein phosphatase 2C isoform beta
Short name:
PP2C-beta
Gene namesi
Name:Ppm1b
Synonyms:Pp2c2, Pppm1b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:101841. Ppm1b.

Subcellular locationi

Cytoplasmcytosol. Membrane
Note: Weakly associates at the membrane and N-myristoylation mediates the membrane localization.

GO - Cellular componenti

  1. cytosol Source: UniProtKB
  2. membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 390389Protein phosphatase 1BPRO_0000057747Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine1 Publication
Cross-linki12 – 12Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15)By similarity

Post-translational modificationi

Isgylation negatively regulates its activity.By similarity
N-myristoylation is essential for the recognition of its substrates for dephosphorylation.1 Publication

Keywords - PTMi

Isopeptide bond, Lipoprotein, Myristate, Ubl conjugation

Proteomic databases

MaxQBiP36993.
PaxDbiP36993.
PRIDEiP36993.

PTM databases

PhosphoSiteiP36993.

Expressioni

Tissue specificityi

Beta-1 is expressed ubiquitously; beta-2 is expressed exclusively in brain and heart; beta-4 is expressed exclusively in brain and intestine; beta-3 and beta-5 are expressed exclusively in testis and intestine.

Gene expression databases

BgeeiP36993.
ExpressionAtlasiP36993. baseline and differential.
GenevestigatoriP36993.

Interactioni

Subunit structurei

Monomer. Interacts with PAK6 (By similarity). Interacts with the phosphorylated form of IKBKB/IKKB (By similarity).By similarity

Protein-protein interaction databases

IntActiP36993. 1 interaction.
MINTiMINT-4108365.

Structurei

3D structure databases

ProteinModelPortaliP36993.
SMRiP36993. Positions 2-373.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PP2C family.Curated

Phylogenomic databases

eggNOGiCOG0631.
GeneTreeiENSGT00740000115384.
HOGENOMiHOG000233895.
HOVERGENiHBG053647.
InParanoidiP36993.
KOiK04461.
OrthoDBiEOG7WMCJH.
PhylomeDBiP36993.

Family and domain databases

Gene3Di1.10.10.430. 1 hit.
3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR012911. PP2C_C.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
PF07830. PP2C_C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81601. SSF81601. 1 hit.
SSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PP2C. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. Align

Note: Isoforms only differ in their C-terminus.

Isoform Beta-1 (identifier: P36993-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGAFLDKPKT EKHNAHGAGN GLRYGLSSMQ GWRVEMEDAH TAVVGIPHGL
60 70 80 90 100
DNWSFFAVYD GHAGSRVANY CSTHLLEHIT TNEDFRAADK SGSALEPSVE
110 120 130 140 150
SVKTGIRTGF LKIDEYMRNF SDLRNGMDRS GSTAVGVMVS PTHMYFINCG
160 170 180 190 200
DSRAVLCRNG QVCFSTQDHK PCNPVEKERI QNAGGSVMIQ RVNGSLAVSR
210 220 230 240 250
ALGDYDYKCV DGKGPTEQLV SPEPEVYEIV RAEEDEFVVL ACDGIWDVMS
260 270 280 290 300
NEELCEFVKS RLEVSDDLEN VCNWVVDTCL HKGSRDNMSV VLVCFSNAPK
310 320 330 340 350
VSEEAVKRDS ELDKHLESRV EEIMQKSGEE GMPDLAHVMR ILSAENIPNL
360 370 380 390
PPGGGLAGKR HVIEAVYSRL NPHKDNDGGA GDLEDSLVAL
Length:390
Mass (Da):42,795
Last modified:June 1, 1994 - v1
Checksum:i255C97B4276189FD
GO
Isoform Beta-2 (identifier: P36993-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     379-390: GAGDLEDSLVAL → VSLHLFPKYLK

Show »
Length:389
Mass (Da):42,980
Checksum:i17938754FC4B2D79
GO
Isoform Beta-3 (identifier: P36993-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     379-390: GAGDLEDSLVAL → FYQPSIAYSDNVFLL

Show »
Length:393
Mass (Da):43,413
Checksum:iBA1DAED2E2CFBFFC
GO
Isoform Beta-4 (identifier: P36993-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     379-390: GAGDLEDSLVAL → MADLSTSICKPS

Show »
Length:390
Mass (Da):42,888
Checksum:iFEFDF945009E835F
GO
Isoform Beta-5 (identifier: P36993-5) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     388-390: VAL → FYQPSIAYSDNVFLL

Show »
Length:402
Mass (Da):44,271
Checksum:i807B4E64B6715E18
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei379 – 39012GAGDL…SLVAL → VSLHLFPKYLK in isoform Beta-2. CuratedVSP_005089Add
BLAST
Alternative sequencei379 – 39012GAGDL…SLVAL → FYQPSIAYSDNVFLL in isoform Beta-3. CuratedVSP_005090Add
BLAST
Alternative sequencei379 – 39012GAGDL…SLVAL → MADLSTSICKPS in isoform Beta-4. CuratedVSP_005091Add
BLAST
Alternative sequencei388 – 3903VAL → FYQPSIAYSDNVFLL in isoform Beta-5. CuratedVSP_005092

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D17411 mRNA. Translation: BAA04233.1.
D17412 mRNA. Translation: BAA04234.1.
D45859 mRNA. Translation: BAA08293.1.
D45860 mRNA. Translation: BAA08294.1.
D45861 mRNA. Translation: BAA08295.1.
U09218 mRNA. Translation: AAB60442.1.
AB007798 Genomic DNA. Translation: BAA84471.1.
CCDSiCCDS37710.1. [P36993-1]
CCDS50200.1. [P36993-3]
CCDS50201.1. [P36993-4]
PIRiI49016.
S65672.
RefSeqiNP_001152969.1. NM_001159497.1. [P36993-4]
NP_001152970.1. NM_001159498.1. [P36993-3]
NP_035281.1. NM_011151.2. [P36993-1]
XP_006523924.1. XM_006523861.1. [P36993-3]
XP_006523925.1. XM_006523862.1. [P36993-4]
UniGeneiMm.249695.

Genome annotation databases

EnsembliENSMUST00000112304; ENSMUSP00000107923; ENSMUSG00000061130. [P36993-1]
ENSMUST00000112305; ENSMUSP00000107924; ENSMUSG00000061130. [P36993-4]
ENSMUST00000112307; ENSMUSP00000107926; ENSMUSG00000061130. [P36993-3]
GeneIDi19043.
KEGGimmu:19043.
UCSCiuc008dtf.2. mouse. [P36993-2]
uc008dth.2. mouse. [P36993-1]
uc008dti.2. mouse. [P36993-4]
uc008dtj.2. mouse. [P36993-3]
uc008dtk.2. mouse. [P36993-5]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D17411 mRNA. Translation: BAA04233.1 .
D17412 mRNA. Translation: BAA04234.1 .
D45859 mRNA. Translation: BAA08293.1 .
D45860 mRNA. Translation: BAA08294.1 .
D45861 mRNA. Translation: BAA08295.1 .
U09218 mRNA. Translation: AAB60442.1 .
AB007798 Genomic DNA. Translation: BAA84471.1 .
CCDSi CCDS37710.1. [P36993-1 ]
CCDS50200.1. [P36993-3 ]
CCDS50201.1. [P36993-4 ]
PIRi I49016.
S65672.
RefSeqi NP_001152969.1. NM_001159497.1. [P36993-4 ]
NP_001152970.1. NM_001159498.1. [P36993-3 ]
NP_035281.1. NM_011151.2. [P36993-1 ]
XP_006523924.1. XM_006523861.1. [P36993-3 ]
XP_006523925.1. XM_006523862.1. [P36993-4 ]
UniGenei Mm.249695.

3D structure databases

ProteinModelPortali P36993.
SMRi P36993. Positions 2-373.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P36993. 1 interaction.
MINTi MINT-4108365.

PTM databases

PhosphoSitei P36993.

Proteomic databases

MaxQBi P36993.
PaxDbi P36993.
PRIDEi P36993.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000112304 ; ENSMUSP00000107923 ; ENSMUSG00000061130 . [P36993-1 ]
ENSMUST00000112305 ; ENSMUSP00000107924 ; ENSMUSG00000061130 . [P36993-4 ]
ENSMUST00000112307 ; ENSMUSP00000107926 ; ENSMUSG00000061130 . [P36993-3 ]
GeneIDi 19043.
KEGGi mmu:19043.
UCSCi uc008dtf.2. mouse. [P36993-2 ]
uc008dth.2. mouse. [P36993-1 ]
uc008dti.2. mouse. [P36993-4 ]
uc008dtj.2. mouse. [P36993-3 ]
uc008dtk.2. mouse. [P36993-5 ]

Organism-specific databases

CTDi 5495.
MGIi MGI:101841. Ppm1b.

Phylogenomic databases

eggNOGi COG0631.
GeneTreei ENSGT00740000115384.
HOGENOMi HOG000233895.
HOVERGENi HBG053647.
InParanoidi P36993.
KOi K04461.
OrthoDBi EOG7WMCJH.
PhylomeDBi P36993.

Enzyme and pathway databases

Reactomei REACT_198533. ISG15 antiviral mechanism.

Miscellaneous databases

NextBioi 295497.
PROi P36993.
SOURCEi Search...

Gene expression databases

Bgeei P36993.
ExpressionAtlasi P36993. baseline and differential.
Genevestigatori P36993.

Family and domain databases

Gene3Di 1.10.10.430. 1 hit.
3.60.40.10. 1 hit.
InterProi IPR001932. PP2C-like_dom.
IPR012911. PP2C_C.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view ]
PANTHERi PTHR13832. PTHR13832. 1 hit.
Pfami PF00481. PP2C. 1 hit.
PF07830. PP2C_C. 1 hit.
[Graphical view ]
SMARTi SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view ]
SUPFAMi SSF81601. SSF81601. 1 hit.
SSF81606. SSF81606. 1 hit.
PROSITEi PS01032. PP2C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of a novel isotype of Mg(2+)-dependent protein phosphatase beta (type 2C beta) enriched in brain and heart."
    Terasawa T., Kobayashi T., Murakami T., Ohnishi M., Kato S., Tanaka O., Kondo H., Yamamoto H., Takeuchi T., Tamura S.
    Arch. Biochem. Biophys. 307:342-349(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Molecular cloning and expression of mouse Mg(2+)-dependent protein phosphatase beta-4 (type 2C beta-4)."
    Kato S., Terasawa T., Kobayashi T., Ohnishi M., Sasahara Y., Kusuda K., Yanagawa Y., Hiraga A., Matsui Y., Tamura S.
    Arch. Biochem. Biophys. 318:387-393(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (BETA-3; BETA-4 AND BETA-5).
    Tissue: Testis.
  3. "Molecular cloning and expression of cDNAs encoding two isoforms of protein phosphatase 2C beta from mouse testis."
    Hou E.W., Kawai Y., Miyasaka H., Li S.S.
    Biochem. Mol. Biol. Int. 32:773-780(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (BETA-3 AND BETA-4).
    Tissue: Testis.
  4. "Alternative promoters direct tissue-specific expression of the mouse protein phosphatase 2Cbeta gene."
    Ohnishi M., Chida N., Kobayashi T., Wang H., Ikeda S., Hanada M., Yanagawa Y., Katsura K., Hiraga A., Tamura S.
    Eur. J. Biochem. 263:736-745(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
  5. "Protein phosphatase 5 modulates SMAD3 function in the transforming growth factor-? pathway."
    Bruce D.L., Macartney T., Yong W., Shou W., Sapkota G.P.
    Cell. Signal. 24:1999-2006(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  6. "N-Myristoylation is essential for protein phosphatases PPM1A and PPM1B to dephosphorylate their physiological substrates in cells."
    Chida T., Ando M., Matsuki T., Masu Y., Nagaura Y., Takano-Yamamoto T., Tamura S., Kobayashi T.
    Biochem. J. 449:741-749(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, MYRISTOYLATION AT GLY-2.

Entry informationi

Entry nameiPPM1B_MOUSE
AccessioniPrimary (citable) accession number: P36993
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: October 29, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3