Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P36993

- PPM1B_MOUSE

UniProt

P36993 - PPM1B_MOUSE

Protein

Protein phosphatase 1B

Gene

Ppm1b

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Enzyme with a broad specificity. Dephosphorylates PRKAA1 and PRKAA2. Inhibits TBK1-mediated antiviral signaling by dephosphorylating it at 'Ser-172'. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.1 Publication

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi60 – 601Manganese 1By similarity
    Metal bindingi60 – 601Manganese 2By similarity
    Metal bindingi61 – 611Manganese 1; via carbonyl oxygenBy similarity
    Metal bindingi243 – 2431Manganese 2By similarity
    Metal bindingi286 – 2861Manganese 2By similarity

    GO - Molecular functioni

    1. magnesium ion binding Source: InterPro
    2. manganese ion binding Source: InterPro
    3. protein serine/threonine phosphatase activity Source: InterPro

    GO - Biological processi

    1. negative regulation of defense response to virus Source: UniProtKB
    2. negative regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
    3. negative regulation of interferon-beta production Source: UniProtKB
    4. negative regulation of NF-kappaB import into nucleus Source: UniProtKB
    5. N-terminal protein myristoylation Source: UniProtKB
    6. protein dephosphorylation Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_198533. ISG15 antiviral mechanism.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein phosphatase 1B (EC:3.1.3.16)
    Alternative name(s):
    Protein phosphatase 2C isoform beta
    Short name:
    PP2C-beta
    Gene namesi
    Name:Ppm1b
    Synonyms:Pp2c2, Pppm1b
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:101841. Ppm1b.

    Subcellular locationi

    Cytoplasmcytosol. Membrane
    Note: Weakly associates at the membrane and N-myristoylation mediates the membrane localization.

    GO - Cellular componenti

    1. cytosol Source: UniProtKB
    2. membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 390389Protein phosphatase 1BPRO_0000057747Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycine1 Publication
    Cross-linki12 – 12Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15)By similarity

    Post-translational modificationi

    Isgylation negatively regulates its activity.By similarity
    N-myristoylation is essential for the recognition of its substrates for dephosphorylation.1 Publication

    Keywords - PTMi

    Isopeptide bond, Lipoprotein, Myristate, Ubl conjugation

    Proteomic databases

    MaxQBiP36993.
    PaxDbiP36993.
    PRIDEiP36993.

    PTM databases

    PhosphoSiteiP36993.

    Expressioni

    Tissue specificityi

    Beta-1 is expressed ubiquitously; beta-2 is expressed exclusively in brain and heart; beta-4 is expressed exclusively in brain and intestine; beta-3 and beta-5 are expressed exclusively in testis and intestine.

    Gene expression databases

    ArrayExpressiP36993.
    BgeeiP36993.
    GenevestigatoriP36993.

    Interactioni

    Subunit structurei

    Monomer. Interacts with PAK6 By similarity. Interacts with the phosphorylated form of IKBKB/IKKB By similarity.By similarity

    Protein-protein interaction databases

    IntActiP36993. 1 interaction.
    MINTiMINT-4108365.

    Structurei

    3D structure databases

    ProteinModelPortaliP36993.
    SMRiP36993. Positions 2-373.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PP2C family.Curated

    Phylogenomic databases

    eggNOGiCOG0631.
    GeneTreeiENSGT00740000115384.
    HOGENOMiHOG000233895.
    HOVERGENiHBG053647.
    KOiK04461.
    OrthoDBiEOG7WMCJH.
    PhylomeDBiP36993.

    Family and domain databases

    Gene3Di1.10.10.430. 1 hit.
    3.60.40.10. 1 hit.
    InterProiIPR001932. PP2C-like_dom.
    IPR012911. PP2C_C.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view]
    PANTHERiPTHR13832. PTHR13832. 1 hit.
    PfamiPF00481. PP2C. 1 hit.
    PF07830. PP2C_C. 1 hit.
    [Graphical view]
    SMARTiSM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view]
    SUPFAMiSSF81601. SSF81601. 1 hit.
    SSF81606. SSF81606. 1 hit.
    PROSITEiPS01032. PP2C. 1 hit.
    [Graphical view]

    Sequences (5)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 5 isoformsi produced by alternative splicing. Align

    Note: Isoforms only differ in their C-terminus.

    Isoform Beta-1 (identifier: P36993-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGAFLDKPKT EKHNAHGAGN GLRYGLSSMQ GWRVEMEDAH TAVVGIPHGL    50
    DNWSFFAVYD GHAGSRVANY CSTHLLEHIT TNEDFRAADK SGSALEPSVE 100
    SVKTGIRTGF LKIDEYMRNF SDLRNGMDRS GSTAVGVMVS PTHMYFINCG 150
    DSRAVLCRNG QVCFSTQDHK PCNPVEKERI QNAGGSVMIQ RVNGSLAVSR 200
    ALGDYDYKCV DGKGPTEQLV SPEPEVYEIV RAEEDEFVVL ACDGIWDVMS 250
    NEELCEFVKS RLEVSDDLEN VCNWVVDTCL HKGSRDNMSV VLVCFSNAPK 300
    VSEEAVKRDS ELDKHLESRV EEIMQKSGEE GMPDLAHVMR ILSAENIPNL 350
    PPGGGLAGKR HVIEAVYSRL NPHKDNDGGA GDLEDSLVAL 390
    Length:390
    Mass (Da):42,795
    Last modified:June 1, 1994 - v1
    Checksum:i255C97B4276189FD
    GO
    Isoform Beta-2 (identifier: P36993-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         379-390: GAGDLEDSLVAL → VSLHLFPKYLK

    Show »
    Length:389
    Mass (Da):42,980
    Checksum:i17938754FC4B2D79
    GO
    Isoform Beta-3 (identifier: P36993-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         379-390: GAGDLEDSLVAL → FYQPSIAYSDNVFLL

    Show »
    Length:393
    Mass (Da):43,413
    Checksum:iBA1DAED2E2CFBFFC
    GO
    Isoform Beta-4 (identifier: P36993-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         379-390: GAGDLEDSLVAL → MADLSTSICKPS

    Show »
    Length:390
    Mass (Da):42,888
    Checksum:iFEFDF945009E835F
    GO
    Isoform Beta-5 (identifier: P36993-5) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         388-390: VAL → FYQPSIAYSDNVFLL

    Show »
    Length:402
    Mass (Da):44,271
    Checksum:i807B4E64B6715E18
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei379 – 39012GAGDL…SLVAL → VSLHLFPKYLK in isoform Beta-2. CuratedVSP_005089Add
    BLAST
    Alternative sequencei379 – 39012GAGDL…SLVAL → FYQPSIAYSDNVFLL in isoform Beta-3. CuratedVSP_005090Add
    BLAST
    Alternative sequencei379 – 39012GAGDL…SLVAL → MADLSTSICKPS in isoform Beta-4. CuratedVSP_005091Add
    BLAST
    Alternative sequencei388 – 3903VAL → FYQPSIAYSDNVFLL in isoform Beta-5. CuratedVSP_005092

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D17411 mRNA. Translation: BAA04233.1.
    D17412 mRNA. Translation: BAA04234.1.
    D45859 mRNA. Translation: BAA08293.1.
    D45860 mRNA. Translation: BAA08294.1.
    D45861 mRNA. Translation: BAA08295.1.
    U09218 mRNA. Translation: AAB60442.1.
    AB007798 Genomic DNA. Translation: BAA84471.1.
    CCDSiCCDS37710.1. [P36993-1]
    CCDS50200.1. [P36993-3]
    CCDS50201.1. [P36993-4]
    PIRiI49016.
    S65672.
    RefSeqiNP_001152969.1. NM_001159497.1. [P36993-4]
    NP_001152970.1. NM_001159498.1. [P36993-3]
    NP_035281.1. NM_011151.2. [P36993-1]
    XP_006523924.1. XM_006523861.1. [P36993-3]
    XP_006523925.1. XM_006523862.1. [P36993-4]
    UniGeneiMm.249695.

    Genome annotation databases

    EnsembliENSMUST00000112304; ENSMUSP00000107923; ENSMUSG00000061130. [P36993-1]
    ENSMUST00000112305; ENSMUSP00000107924; ENSMUSG00000061130. [P36993-4]
    ENSMUST00000112307; ENSMUSP00000107926; ENSMUSG00000061130. [P36993-3]
    GeneIDi19043.
    KEGGimmu:19043.
    UCSCiuc008dtf.2. mouse. [P36993-2]
    uc008dth.2. mouse. [P36993-1]
    uc008dti.2. mouse. [P36993-4]
    uc008dtj.2. mouse. [P36993-3]
    uc008dtk.2. mouse. [P36993-5]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D17411 mRNA. Translation: BAA04233.1 .
    D17412 mRNA. Translation: BAA04234.1 .
    D45859 mRNA. Translation: BAA08293.1 .
    D45860 mRNA. Translation: BAA08294.1 .
    D45861 mRNA. Translation: BAA08295.1 .
    U09218 mRNA. Translation: AAB60442.1 .
    AB007798 Genomic DNA. Translation: BAA84471.1 .
    CCDSi CCDS37710.1. [P36993-1 ]
    CCDS50200.1. [P36993-3 ]
    CCDS50201.1. [P36993-4 ]
    PIRi I49016.
    S65672.
    RefSeqi NP_001152969.1. NM_001159497.1. [P36993-4 ]
    NP_001152970.1. NM_001159498.1. [P36993-3 ]
    NP_035281.1. NM_011151.2. [P36993-1 ]
    XP_006523924.1. XM_006523861.1. [P36993-3 ]
    XP_006523925.1. XM_006523862.1. [P36993-4 ]
    UniGenei Mm.249695.

    3D structure databases

    ProteinModelPortali P36993.
    SMRi P36993. Positions 2-373.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P36993. 1 interaction.
    MINTi MINT-4108365.

    PTM databases

    PhosphoSitei P36993.

    Proteomic databases

    MaxQBi P36993.
    PaxDbi P36993.
    PRIDEi P36993.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000112304 ; ENSMUSP00000107923 ; ENSMUSG00000061130 . [P36993-1 ]
    ENSMUST00000112305 ; ENSMUSP00000107924 ; ENSMUSG00000061130 . [P36993-4 ]
    ENSMUST00000112307 ; ENSMUSP00000107926 ; ENSMUSG00000061130 . [P36993-3 ]
    GeneIDi 19043.
    KEGGi mmu:19043.
    UCSCi uc008dtf.2. mouse. [P36993-2 ]
    uc008dth.2. mouse. [P36993-1 ]
    uc008dti.2. mouse. [P36993-4 ]
    uc008dtj.2. mouse. [P36993-3 ]
    uc008dtk.2. mouse. [P36993-5 ]

    Organism-specific databases

    CTDi 5495.
    MGIi MGI:101841. Ppm1b.

    Phylogenomic databases

    eggNOGi COG0631.
    GeneTreei ENSGT00740000115384.
    HOGENOMi HOG000233895.
    HOVERGENi HBG053647.
    KOi K04461.
    OrthoDBi EOG7WMCJH.
    PhylomeDBi P36993.

    Enzyme and pathway databases

    Reactomei REACT_198533. ISG15 antiviral mechanism.

    Miscellaneous databases

    NextBioi 295497.
    PROi P36993.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P36993.
    Bgeei P36993.
    Genevestigatori P36993.

    Family and domain databases

    Gene3Di 1.10.10.430. 1 hit.
    3.60.40.10. 1 hit.
    InterProi IPR001932. PP2C-like_dom.
    IPR012911. PP2C_C.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view ]
    PANTHERi PTHR13832. PTHR13832. 1 hit.
    Pfami PF00481. PP2C. 1 hit.
    PF07830. PP2C_C. 1 hit.
    [Graphical view ]
    SMARTi SM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81601. SSF81601. 1 hit.
    SSF81606. SSF81606. 1 hit.
    PROSITEi PS01032. PP2C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of a novel isotype of Mg(2+)-dependent protein phosphatase beta (type 2C beta) enriched in brain and heart."
      Terasawa T., Kobayashi T., Murakami T., Ohnishi M., Kato S., Tanaka O., Kondo H., Yamamoto H., Takeuchi T., Tamura S.
      Arch. Biochem. Biophys. 307:342-349(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Molecular cloning and expression of mouse Mg(2+)-dependent protein phosphatase beta-4 (type 2C beta-4)."
      Kato S., Terasawa T., Kobayashi T., Ohnishi M., Sasahara Y., Kusuda K., Yanagawa Y., Hiraga A., Matsui Y., Tamura S.
      Arch. Biochem. Biophys. 318:387-393(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (BETA-3; BETA-4 AND BETA-5).
      Tissue: Testis.
    3. "Molecular cloning and expression of cDNAs encoding two isoforms of protein phosphatase 2C beta from mouse testis."
      Hou E.W., Kawai Y., Miyasaka H., Li S.S.
      Biochem. Mol. Biol. Int. 32:773-780(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (BETA-3 AND BETA-4).
      Tissue: Testis.
    4. "Alternative promoters direct tissue-specific expression of the mouse protein phosphatase 2Cbeta gene."
      Ohnishi M., Chida N., Kobayashi T., Wang H., Ikeda S., Hanada M., Yanagawa Y., Katsura K., Hiraga A., Tamura S.
      Eur. J. Biochem. 263:736-745(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/Sv.
    5. "Protein phosphatase 5 modulates SMAD3 function in the transforming growth factor-? pathway."
      Bruce D.L., Macartney T., Yong W., Shou W., Sapkota G.P.
      Cell. Signal. 24:1999-2006(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    6. "N-Myristoylation is essential for protein phosphatases PPM1A and PPM1B to dephosphorylate their physiological substrates in cells."
      Chida T., Ando M., Matsuki T., Masu Y., Nagaura Y., Takano-Yamamoto T., Tamura S., Kobayashi T.
      Biochem. J. 449:741-749(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, MYRISTOYLATION AT GLY-2.

    Entry informationi

    Entry nameiPPM1B_MOUSE
    AccessioniPrimary (citable) accession number: P36993
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3