Protein phosphatase 1B - Mus musculus (Mouse)

Contribute

Send feedback

Read comments (?) or add your own

P36993 (PPM1B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 108. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein phosphatase 1B
EC=3.1.3.16

Alternative name(s):
Protein phosphatase 2C isoform beta
Short name=PP2C-beta

Gene names

Name:	Ppm1b
Synonyms:	Pp2c2, Pppm1b

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Enzyme with a broad specificity.
Catalytic activity	A phosphoprotein + H₂O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Subunit structure	Monomer. Interacts with PAK6 By similarity.
Tissue specificity	Beta-1 is expressed ubiquitously; beta-2 is expressed exclusively in brain and heart; beta-4 is expressed exclusively in brain and intestine; beta-3 and beta-5 are expressed exclusively in testis and intestine.
Sequence similarities	Belongs to the PP2C family.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein dephosphorylation Inferred from electronic annotation. Source: InterPro
Molecular_function	magnesium ion binding Inferred from electronic annotation. Source: InterPro manganese ion binding Inferred from electronic annotation. Source: InterPro protein serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select] Note: Isoforms only differ in their C-terminus.

Isoform Beta-1 (identifier: P36993-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Beta-2 (identifier: P36993-2) The sequence of this isoform differs from the canonical sequence as follows: 379-390: GAGDLEDSLVAL → VSLHLFPKYLK

Isoform Beta-3 (identifier: P36993-3) The sequence of this isoform differs from the canonical sequence as follows: 379-390: GAGDLEDSLVAL → FYQPSIAYSDNVFLL

Isoform Beta-4 (identifier: P36993-4) The sequence of this isoform differs from the canonical sequence as follows: 379-390: GAGDLEDSLVAL → MADLSTSICKPS

Isoform Beta-5 (identifier: P36993-5) The sequence of this isoform differs from the canonical sequence as follows: 388-390: VAL → FYQPSIAYSDNVFLL

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 390

390

Protein phosphatase 1B

PRO_0000057747

Sites

Metal binding

Manganese 1 By similarity

Metal binding

Manganese 2 By similarity

Metal binding

Manganese 1; via carbonyl oxygen By similarity

Metal binding

243

Manganese 2 By similarity

Metal binding

286

Manganese 2 By similarity

Natural variations

Alternative sequence

379 – 390

GAGDL…SLVAL → VSLHLFPKYLK in isoform Beta-2.

VSP_005089

Alternative sequence

379 – 390

GAGDL…SLVAL → FYQPSIAYSDNVFLL in isoform Beta-3.

VSP_005090

Alternative sequence

379 – 390

GAGDL…SLVAL → MADLSTSICKPS in isoform Beta-4.

VSP_005091

Alternative sequence

388 – 390

VAL → FYQPSIAYSDNVFLL in isoform Beta-5.

VSP_005092

Sequences

Sequence		Length	Mass (Da)
Isoform Beta-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 255C97B4276189FD Show »	FASTA	390	42,795
10 20 30 40 50 60 MGAFLDKPKT EKHNAHGAGN GLRYGLSSMQ GWRVEMEDAH TAVVGIPHGL DNWSFFAVYD 70 80 90 100 110 120 GHAGSRVANY CSTHLLEHIT TNEDFRAADK SGSALEPSVE SVKTGIRTGF LKIDEYMRNF 130 140 150 160 170 180 SDLRNGMDRS GSTAVGVMVS PTHMYFINCG DSRAVLCRNG QVCFSTQDHK PCNPVEKERI 190 200 210 220 230 240 QNAGGSVMIQ RVNGSLAVSR ALGDYDYKCV DGKGPTEQLV SPEPEVYEIV RAEEDEFVVL 250 260 270 280 290 300 ACDGIWDVMS NEELCEFVKS RLEVSDDLEN VCNWVVDTCL HKGSRDNMSV VLVCFSNAPK 310 320 330 340 350 360 VSEEAVKRDS ELDKHLESRV EEIMQKSGEE GMPDLAHVMR ILSAENIPNL PPGGGLAGKR 370 380 390 HVIEAVYSRL NPHKDNDGGA GDLEDSLVAL « Hide
Isoform Beta-2 [UniParc]. Checksum: 17938754FC4B2D79 Show »	FASTA	389	42,980
10 20 30 40 50 60 MGAFLDKPKT EKHNAHGAGN GLRYGLSSMQ GWRVEMEDAH TAVVGIPHGL DNWSFFAVYD 70 80 90 100 110 120 GHAGSRVANY CSTHLLEHIT TNEDFRAADK SGSALEPSVE SVKTGIRTGF LKIDEYMRNF 130 140 150 160 170 180 SDLRNGMDRS GSTAVGVMVS PTHMYFINCG DSRAVLCRNG QVCFSTQDHK PCNPVEKERI 190 200 210 220 230 240 QNAGGSVMIQ RVNGSLAVSR ALGDYDYKCV DGKGPTEQLV SPEPEVYEIV RAEEDEFVVL 250 260 270 280 290 300 ACDGIWDVMS NEELCEFVKS RLEVSDDLEN VCNWVVDTCL HKGSRDNMSV VLVCFSNAPK 310 320 330 340 350 360 VSEEAVKRDS ELDKHLESRV EEIMQKSGEE GMPDLAHVMR ILSAENIPNL PPGGGLAGKR 370 380 HVIEAVYSRL NPHKDNDGVS LHLFPKYLK « Hide
Isoform Beta-3 [UniParc]. Checksum: BA1DAED2E2CFBFFC Show »	FASTA	393	43,413
10 20 30 40 50 60 MGAFLDKPKT EKHNAHGAGN GLRYGLSSMQ GWRVEMEDAH TAVVGIPHGL DNWSFFAVYD 70 80 90 100 110 120 GHAGSRVANY CSTHLLEHIT TNEDFRAADK SGSALEPSVE SVKTGIRTGF LKIDEYMRNF 130 140 150 160 170 180 SDLRNGMDRS GSTAVGVMVS PTHMYFINCG DSRAVLCRNG QVCFSTQDHK PCNPVEKERI 190 200 210 220 230 240 QNAGGSVMIQ RVNGSLAVSR ALGDYDYKCV DGKGPTEQLV SPEPEVYEIV RAEEDEFVVL 250 260 270 280 290 300 ACDGIWDVMS NEELCEFVKS RLEVSDDLEN VCNWVVDTCL HKGSRDNMSV VLVCFSNAPK 310 320 330 340 350 360 VSEEAVKRDS ELDKHLESRV EEIMQKSGEE GMPDLAHVMR ILSAENIPNL PPGGGLAGKR 370 380 390 HVIEAVYSRL NPHKDNDGFY QPSIAYSDNV FLL « Hide
Isoform Beta-4 [UniParc]. Checksum: FEFDF945009E835F Show »	FASTA	390	42,888
10 20 30 40 50 60 MGAFLDKPKT EKHNAHGAGN GLRYGLSSMQ GWRVEMEDAH TAVVGIPHGL DNWSFFAVYD 70 80 90 100 110 120 GHAGSRVANY CSTHLLEHIT TNEDFRAADK SGSALEPSVE SVKTGIRTGF LKIDEYMRNF 130 140 150 160 170 180 SDLRNGMDRS GSTAVGVMVS PTHMYFINCG DSRAVLCRNG QVCFSTQDHK PCNPVEKERI 190 200 210 220 230 240 QNAGGSVMIQ RVNGSLAVSR ALGDYDYKCV DGKGPTEQLV SPEPEVYEIV RAEEDEFVVL 250 260 270 280 290 300 ACDGIWDVMS NEELCEFVKS RLEVSDDLEN VCNWVVDTCL HKGSRDNMSV VLVCFSNAPK 310 320 330 340 350 360 VSEEAVKRDS ELDKHLESRV EEIMQKSGEE GMPDLAHVMR ILSAENIPNL PPGGGLAGKR 370 380 390 HVIEAVYSRL NPHKDNDGMA DLSTSICKPS « Hide
Isoform Beta-5 [UniParc]. Checksum: 807B4E64B6715E18 Show »	FASTA	402	44,271
10 20 30 40 50 60 MGAFLDKPKT EKHNAHGAGN GLRYGLSSMQ GWRVEMEDAH TAVVGIPHGL DNWSFFAVYD 70 80 90 100 110 120 GHAGSRVANY CSTHLLEHIT TNEDFRAADK SGSALEPSVE SVKTGIRTGF LKIDEYMRNF 130 140 150 160 170 180 SDLRNGMDRS GSTAVGVMVS PTHMYFINCG DSRAVLCRNG QVCFSTQDHK PCNPVEKERI 190 200 210 220 230 240 QNAGGSVMIQ RVNGSLAVSR ALGDYDYKCV DGKGPTEQLV SPEPEVYEIV RAEEDEFVVL 250 260 270 280 290 300 ACDGIWDVMS NEELCEFVKS RLEVSDDLEN VCNWVVDTCL HKGSRDNMSV VLVCFSNAPK 310 320 330 340 350 360 VSEEAVKRDS ELDKHLESRV EEIMQKSGEE GMPDLAHVMR ILSAENIPNL PPGGGLAGKR 370 380 390 400 HVIEAVYSRL NPHKDNDGGA GDLEDSLFYQ PSIAYSDNVF LL « Hide

References

[1]	"Molecular cloning of a novel isotype of Mg(2+)-dependent protein phosphatase beta (type 2C beta) enriched in brain and heart." Terasawa T., Kobayashi T., Murakami T., Ohnishi M., Kato S., Tanaka O., Kondo H., Yamamoto H., Takeuchi T., Tamura S. Arch. Biochem. Biophys. 307:342-349(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular cloning and expression of mouse Mg(2+)-dependent protein phosphatase beta-4 (type 2C beta-4)." Kato S., Terasawa T., Kobayashi T., Ohnishi M., Sasahara Y., Kusuda K., Yanagawa Y., Hiraga A., Matsui Y., Tamura S. Arch. Biochem. Biophys. 318:387-393(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (BETA-3; BETA-4 AND BETA-5). Tissue: Testis.
[3]	"Molecular cloning and expression of cDNAs encoding two isoforms of protein phosphatase 2C beta from mouse testis." Hou E.W., Kawai Y., Miyasaka H., Li S.S. Biochem. Mol. Biol. Int. 32:773-780(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (BETA-3 AND BETA-4). Tissue: Testis.
[4]	"Alternative promoters direct tissue-specific expression of the mouse protein phosphatase 2Cbeta gene." Ohnishi M., Chida N., Kobayashi T., Wang H., Ikeda S., Hanada M., Yanagawa Y., Katsura K., Hiraga A., Tamura S. Eur. J. Biochem. 263:736-745(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D17411 mRNA. Translation: BAA04233.1. D17412 mRNA. Translation: BAA04234.1. D45859 mRNA. Translation: BAA08293.1. D45860 mRNA. Translation: BAA08294.1. D45861 mRNA. Translation: BAA08295.1. U09218 mRNA. Translation: AAB60442.1. AB007798 Genomic DNA. Translation: BAA84471.1.
IPI	IPI00222989. IPI00399550. IPI00466073. IPI00854950. IPI00876176.
PIR	I49016. S65672.
RefSeq	NP_001152969.1. NM_001159497.1. NP_001152970.1. NM_001159498.1. NP_035281.1. NM_011151.2.
UniGene	Mm.249695.
3D structure databases
ProteinModelPortal	P36993.
SMR	P36993. Positions 2-373.
ModBase	Search...
PTM databases
PhosphoSite	P36993.
Proteomic databases
PaxDb	P36993.
PRIDE	P36993.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000112304; ENSMUSP00000107923; ENSMUSG00000061130. ENSMUST00000112305; ENSMUSP00000107924; ENSMUSG00000061130. ENSMUST00000112307; ENSMUSP00000107926; ENSMUSG00000061130.
GeneID	19043.
KEGG	mmu:19043.
UCSC	uc008dtf.2. mouse. uc008dti.2. mouse. uc008dtj.2. mouse. uc008dtk.2. mouse.
Organism-specific databases
CTD	5495.
MGI	MGI:101841. Ppm1b.
Phylogenomic databases
eggNOG	COG0631.
GeneTree	ENSGT00650000093052.
HOGENOM	HOG000233895.
HOVERGEN	HBG053647.
KO	K04461.
OrthoDB	EOG4BP1BZ.
Gene expression databases
ArrayExpress	P36993.
Bgee	P36993.
Genevestigator	P36993.
GermOnline	ENSMUSG00000061130. Mus musculus.
Family and domain databases
Gene3D	1.10.10.430. 1 hit. 3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR012911. PP2C_C. IPR000222. PP2C_Mn2_Asp60_BS. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. PF07830. PP2C_C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit. SSF81601. PP2C_C. 1 hit.
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	295497.
SOURCE	Search...

Entry information

Entry name

PPM1B_MOUSE

Accession

Primary (citable) accession number: P36993

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Sites

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents