P36975 (SNP25_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Synaptosomal-associated protein 25
Short name=SNAP-25

Alternative name(s):
Synaptosomal-associated 25 kDa protein

Gene names

Name:	Snap25
ORF Names:	CG40452

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion.
Subcellular location	Cell junction › synapse › synaptosome By similarity. Note: Complexed with macromolecular elements of the nerve terminal.
Tissue specificity	Exclusively found in brain and ganglia.
Sequence similarities	Belongs to the SNAP-25 family. Contains 2 t-SNARE coiled-coil homology domains.

Ontologies

Keywords
Cellular component	Cell junction Synapse Synaptosome
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of short-term neuronal synaptic plasticity Inferred from direct assay. Source: FlyBase synaptic vesicle docking involved in exocytosis Non-traceable author statement. Source: FlyBase synaptic vesicle priming Inferred from direct assay. Source: FlyBase
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Non-traceable author statement. Source: FlyBase synapse Inferred from electronic annotation. Source: UniProtKB-KW synaptosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	SNAP receptor activity Non-traceable author statement. Source: FlyBase
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: P36975-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: P36975-2)

The sequence of this isoform differs from the canonical sequence as follows:
     2-61: PADPSEEVAPQVPKTELEELQINAQGVADESLESTRRMLALCEESKEAGIRTLVALDDQG → R

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 212	212	Synaptosomal-associated protein 25		PRO_0000213594
Regions
	Domain	26 – 88	63	t-SNARE coiled-coil homology 1
	Domain	148 – 210	63	t-SNARE coiled-coil homology 2
	Compositional bias	91 – 99	9	Cys-rich
Natural variations
	Alternative sequence	2 – 61	60	PADPS…LDDQG → R in isoform B.		VSP_022126

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified June 1, 1994. Version 1. Checksum: BDC90649A1AF3AC8	FASTA	212	23,685
	10 20 30 40 50 60 MPADPSEEVA PQVPKTELEE LQINAQGVAD ESLESTRRML ALCEESKEAG IRTLVALDDQ 70 80 90 100 110 120 GEQLDRIEEG MDQINADMRE AEKNLSGMEK CCGICVLPCN KSQSFKEDDG TWKGNDDGKV 130 140 150 160 170 180 VNNQPQRVMD DRNGMMAQAG YIGRITNDAR EDEMEENMGQ VNTMIGNLRN MALDMGSELE 190 200 210 NQNRQIDRIN RKGESNEARI AVANQRAHQL LK
	Isoform B [UniParc]. Checksum: 3C95F54CCD764161	FASTA	153	17,380
	10 20 30 40 50 60 MREQLDRIEE GMDQINADMR EAEKNLSGME KCCGICVLPC NKSQSFKEDD GTWKGNDDGK 70 80 90 100 110 120 VVNNQPQRVM DDRNGMMAQA GYIGRITNDA REDEMEENMG QVNTMIGNLR NMALDMGSEL 130 140 150 ENQNRQIDRI NRKGESNEAR IAVANQRAHQ LLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evolutionary conservation of synaptosome-associated protein 25 kDa (SNAP-25) shown by Drosophila and Torpedo cDNA clones." Risinger C., Blomqvist A.G., Lundell I., Lambertsson A., Nassel D., Pieribone V.A., Brodin L., Larhammar D. J. Biol. Chem. 268:24408-24414(1993) [PubMed: 8226991] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A). Tissue: Head.
[2]	"Complex gene organization of synaptic protein SNAP-25 in Drosophila melanogaster." Risinger C., Deitcher D.L., Lundell I., Schwarz T.L., Larhammar D. Gene 194:169-177(1997) [PubMed: 9272858] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[5]	"Heterochromatic sequences in a Drosophila whole-genome shotgun assembly." Hoskins R.A., Smith C.D., Carlson J.W., Carvalho A.B., Halpern A., Kaminker J.S., Kennedy C., Mungall C.J., Sullivan B.A., Sutton G.G., Yasuhara J.C., Wakimoto B.T., Myers E.W., Celniker S.E., Rubin G.M., Karpen G.H. Genome Biol. 3:RESEARCH0085.1-RESEARCH0085.16(2002) [PubMed: 12537574] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[6]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Berkeley. Tissue: Head.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L22021 mRNA. Translation: AAA16059.1. U81153 , U81147, U81148, U81149, U81150, U81151, U81152 Genomic DNA. Translation: AAB39757.1. AE014296 Genomic DNA. Translation: EAA46071.2. AE014296 Genomic DNA. Translation: EAL24571.1. BT023789 mRNA. Translation: AAZ41798.1.
RefSeq	NP_001036641.1. NM_001043176.1.
UniGene	Dm.33412.
3D structure databases
ProteinModelPortal	P36975.
SMR	P36975. Positions 17-85, 149-207.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-23273N.
MINT	MINT-1714865.
STRING	P36975.
Proteomic databases
PRIDE	P36975.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0111143; FBpp0110435; FBgn0011288.
GeneID	3355084.
KEGG	dme:Dmel_CG40452.
Organism-specific databases
CTD	6616.
FlyBase	FBgn0011288. Snap25.
Phylogenomic databases
eggNOG	inNOG07049.
GeneTree	EMGT00050000012066.
InParanoid	P36975.
OMA	ESADAYQ.
OrthoDB	EOG45HQDD.
PhylomeDB	P36975.
Gene expression databases
Bgee	P36975.
Family and domain databases
InterPro	IPR000928. SNAP-25. IPR000727. T_SNARE_dom. [Graphical view]
KO	K08508.
Pfam	PF00835. SNAP-25. 1 hit. PF05739. SNARE. 1 hit. [Graphical view]
SMART	SM00397. t_SNARE. 2 hits. [Graphical view]
PROSITE	PS50192. T_SNARE. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	850492.

Entry information

Entry name

SNP25_DROME

Accession

Primary (citable) accession number: P36975
Secondary accession number(s): Q5LJU6, Q7PLV2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	January 25, 2012
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project