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Protein

Synaptosomal-associated protein 25

Gene

Snap25

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion (By similarity).By similarity

GO - Molecular functioni

  • SNAP receptor activity Source: FlyBase
  • SNARE binding Source: FlyBase
  • syntaxin binding Source: GO_Central

GO - Biological processi

  • Golgi to plasma membrane transport Source: FlyBase
  • neuromuscular synaptic transmission Source: FlyBase
  • neurotransmitter secretion Source: FlyBase
  • regulation of short-term neuronal synaptic plasticity Source: FlyBase
  • synaptic transmission Source: FlyBase
  • synaptic vesicle docking Source: FlyBase
  • synaptic vesicle fusion to presynaptic active zone membrane Source: FlyBase
  • synaptic vesicle priming Source: FlyBase
  • synaptic vesicle transport Source: FlyBase
  • vesicle fusion Source: FlyBase
  • vesicle-mediated transport Source: FlyBase
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptosomal-associated protein 25
Short name:
SNAP-25
Alternative name(s):
Synaptosomal-associated 25 kDa protein
Gene namesi
Name:Snap25
ORF Names:CG40452
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0011288. Snap25.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cytoplasm Source: UniProtKB
  • cytosol Source: GOC
  • membrane Source: UniProtKB
  • plasma membrane Source: FlyBase
  • SNARE complex Source: UniProtKB
  • synapse Source: FlyBase
  • terminal bouton Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 212212Synaptosomal-associated protein 25PRO_0000213594Add
BLAST

Proteomic databases

PaxDbiP36975.
PRIDEiP36975.

Expressioni

Tissue specificityi

Exclusively found in brain and ganglia.1 Publication

Gene expression databases

BgeeiP36975.
GenevisibleiP36975. DM.

Interactioni

GO - Molecular functioni

  • SNAP receptor activity Source: FlyBase
  • SNARE binding Source: FlyBase
  • syntaxin binding Source: GO_Central

Protein-protein interaction databases

BioGridi78209. 22 interactions.
DIPiDIP-23273N.
MINTiMINT-1714865.
STRINGi7227.FBpp0110435.

Structurei

3D structure databases

ProteinModelPortaliP36975.
SMRiP36975. Positions 17-85, 139-211.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 8863t-SNARE coiled-coil homology 1PROSITE-ProRule annotationAdd
BLAST
Domaini148 – 21063t-SNARE coiled-coil homology 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi91 – 999Cys-rich

Sequence similaritiesi

Belongs to the SNAP-25 family.Curated
Contains 2 t-SNARE coiled-coil homology domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG3065. Eukaryota.
ENOG410Y3Y0. LUCA.
GeneTreeiENSGT00390000012186.
InParanoidiP36975.
KOiK18211.
OMAiMDERNGM.
OrthoDBiEOG75F4F5.
PhylomeDBiP36975.

Family and domain databases

InterProiIPR000928. SNAP-25.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamiPF00835. SNAP-25. 1 hit.
[Graphical view]
SMARTiSM00397. t_SNARE. 2 hits.
[Graphical view]
PROSITEiPS50192. T_SNARE. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P36975-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPADPSEEVA PQVPKTELEE LQINAQGVAD ESLESTRRML ALCEESKEAG
60 70 80 90 100
IRTLVALDDQ GEQLDRIEEG MDQINADMRE AEKNLSGMEK CCGICVLPCN
110 120 130 140 150
KSQSFKEDDG TWKGNDDGKV VNNQPQRVMD DRNGMMAQAG YIGRITNDAR
160 170 180 190 200
EDEMEENMGQ VNTMIGNLRN MALDMGSELE NQNRQIDRIN RKGESNEARI
210
AVANQRAHQL LK
Length:212
Mass (Da):23,685
Last modified:June 1, 1994 - v1
Checksum:iBDC90649A1AF3AC8
GO
Isoform C (identifier: P36975-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     47-59: KEAGIRTLVALDD → AEVGMRSIVMLDE

Note: No experimental confirmation available.
Show »
Length:212
Mass (Da):23,734
Checksum:iACF9C175C77DC98C
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei47 – 5913KEAGI…VALDD → AEVGMRSIVMLDE in isoform C. CuratedVSP_054454Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22021 mRNA. Translation: AAA16059.1.
U81153
, U81147, U81148, U81149, U81150, U81151, U81152 Genomic DNA. Translation: AAB39757.1.
AE014296 Genomic DNA. Translation: EAA46071.2.
AE014296 Genomic DNA. Translation: EAL24571.2.
BT023789 mRNA. Translation: AAZ41798.1.
BT126217 mRNA. Translation: AEB22096.1.
RefSeqiNP_001036641.1. NM_001043176.2.
NP_001036642.2. NM_001043177.3. [P36975-3]
UniGeneiDm.33412.

Genome annotation databases

EnsemblMetazoaiFBtr0111143; FBpp0110435; FBgn0011288. [P36975-1]
GeneIDi3355084.
KEGGidme:Dmel_CG40452.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22021 mRNA. Translation: AAA16059.1.
U81153
, U81147, U81148, U81149, U81150, U81151, U81152 Genomic DNA. Translation: AAB39757.1.
AE014296 Genomic DNA. Translation: EAA46071.2.
AE014296 Genomic DNA. Translation: EAL24571.2.
BT023789 mRNA. Translation: AAZ41798.1.
BT126217 mRNA. Translation: AEB22096.1.
RefSeqiNP_001036641.1. NM_001043176.2.
NP_001036642.2. NM_001043177.3. [P36975-3]
UniGeneiDm.33412.

3D structure databases

ProteinModelPortaliP36975.
SMRiP36975. Positions 17-85, 139-211.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi78209. 22 interactions.
DIPiDIP-23273N.
MINTiMINT-1714865.
STRINGi7227.FBpp0110435.

Proteomic databases

PaxDbiP36975.
PRIDEiP36975.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0111143; FBpp0110435; FBgn0011288. [P36975-1]
GeneIDi3355084.
KEGGidme:Dmel_CG40452.

Organism-specific databases

CTDi6616.
FlyBaseiFBgn0011288. Snap25.

Phylogenomic databases

eggNOGiKOG3065. Eukaryota.
ENOG410Y3Y0. LUCA.
GeneTreeiENSGT00390000012186.
InParanoidiP36975.
KOiK18211.
OMAiMDERNGM.
OrthoDBiEOG75F4F5.
PhylomeDBiP36975.

Miscellaneous databases

ChiTaRSiSnap25. fly.
GenomeRNAii3355084.
PROiP36975.

Gene expression databases

BgeeiP36975.
GenevisibleiP36975. DM.

Family and domain databases

InterProiIPR000928. SNAP-25.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamiPF00835. SNAP-25. 1 hit.
[Graphical view]
SMARTiSM00397. t_SNARE. 2 hits.
[Graphical view]
PROSITEiPS50192. T_SNARE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Evolutionary conservation of synaptosome-associated protein 25 kDa (SNAP-25) shown by Drosophila and Torpedo cDNA clones."
    Risinger C., Blomqvist A.G., Lundell I., Lambertsson A., Nassel D., Pieribone V.A., Brodin L., Larhammar D.
    J. Biol. Chem. 268:24408-24414(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), TISSUE SPECIFICITY.
    Tissue: Head.
  2. "Complex gene organization of synaptic protein SNAP-25 in Drosophila melanogaster."
    Risinger C., Deitcher D.L., Lundell I., Schwarz T.L., Larhammar D.
    Gene 194:169-177(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  5. Stapleton M., Booth B., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E.
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
    Strain: Berkeley.
    Tissue: Head.

Entry informationi

Entry nameiSNP25_DROME
AccessioniPrimary (citable) accession number: P36975
Secondary accession number(s): F3YD56, Q5LJU6, Q7PLV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: July 6, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.