Reviewed, UniProtKB/Swiss-Prot P36973 (APT2_YEAST)

Names and origin

Protein names

Recommended name:
    Adenine phosphoribosyltransferase 2
      Short name=APRT 2
    EC=2.4.2.7

Gene names

Name:	APT2
Ordered Locus Names:	YDR441C
ORF Names:	D9461.27

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis. May lack catalytic activity.
Catalytic activity	AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate. Ref.3
Pathway	Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.
Subcellular location	Cytoplasm.
Miscellaneous	Present with 937 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family.

Ontologies

Keywords
Biological process	Purine salvage
Cellular component	Cytoplasm
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	adenine salvage Inferred from electronic annotation. Source: InterPro purine ribonucleoside salvage Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: SGD
Molecular function	adenine phosphoribosyltransferase activity Ref.3 Inferred from electronic annotation. Source: EC identical protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-2738,EBI-2738
TEF1	P02994	1	EBI-2738,EBI-6314

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 181	181	Adenine phosphoribosyltransferase 2		PRO_0000149518
Experimental info
	Sequence conflict	57	1	A → G in AAB64883. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P36973-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 58AB4D41380DFEB3	FASTA	181	20,000
	10 20 30 40 50 60 MSISESYAKE IKTAFRQFTD FPIEGEQFED FLPIIGNPTL FQKLVHTFKT HLEEKFAKEK 70 80 90 100 110 120 IDFIAGIEAR GLLFGPSLAL ALGVGFVPIR RVGKLPGECA SITFTKLDHE EIFEMQVEAI 130 140 150 160 170 180 PFDSNVVVVD DVLATGGTAY AAGDLIRQVG AHILEYDFVL VLDSLHGEEK LSAPIFSILH S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A Saccharomyces cerevisiae gene encoding a potential adenine phosphoribosyltransferase." Yuryev A., Corden J.L. Yeast 10:659-662(1994) [PubMed: 7941749] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"APT1, but not APT2, codes for a functional adenine phosphoribosyltransferase in Saccharomyces cerevisiae." Alfonzo J.D., Crother T.R., Guetsova M.L., Daignan-Fornier B., Taylor M.W. J. Bacteriol. 181:347-352(1999) [PubMed: 9864350] [Abstract] Cited for: POSSIBLE LACK OF CATALYTIC ACTIVITY.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L14434 Genomic DNA. Translation: AAA62848.1. U33007 Genomic DNA. Translation: AAB64883.1.
PIR	S69721.
RefSeq	NP_010729.1.
3D structure databases
SMR	P36973. Positions 1-180.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2056N.
IntAct	P36973. 7 interactions.
STRING	P36973.
Genome annotation databases
Ensembl	YDR441C; YDR441C; YDR441C; Saccharomyces cerevisiae. [Genome view]
GeneID	852051.
KEGG	sce:YDR441C.
Organism-specific databases
CYGD	YDR441c.
SGD	S000002849. APT2.
Phylogenomic databases
eggNOG	fuNOG04141.
HOGENOM	HBG703830.
OrthoDB	EOG9W3V4P.
PhylomeDB	P36973.
Enzyme and pathway databases
BRENDA	2.4.2.7. 250.
Gene expression databases
ArrayExpress	P36973.
Genevestigator	P36973.
GermOnline	YDR441C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005764. Ade_phspho_trans. IPR000836. PRibTrfase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

APT2_YEAST

Accession

Primary (citable) accession number: P36973
Secondary accession number(s): Q04090

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	January 19, 2010
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)