Phospholipid hydroperoxide glutathione peroxidase, mitochondrial precursor

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P36970 (GPX41_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 102. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
Short name=PHGPx
EC=1.11.1.12

Alternative name(s):
Glutathione peroxidase 4
Short name=GPx-4
Short name=GSHPx-4

Gene names

Name:

Gpx4

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	197 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage By similarity.
Catalytic activity	2 glutathione + a lipid hydroperoxide = glutathione disulfide + lipid + 2 H₂O.
Subcellular location	Mitochondrion. Cytoplasm.
Tissue specificity	Present primarily in testis. Expressed in flagella of epididymal sperm. Ref.6
Sequence similarities	Belongs to the glutathione peroxidase family.

Ontologies

Keywords
Cellular component	Cytoplasm Mitochondrion
Coding sequence diversity	Alternative initiation Alternative splicing Selenocysteine
Domain	Transit peptide
Molecular function	Developmental protein Oxidoreductase Peroxidase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW response to oxidative stress Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glutathione peroxidase activity Inferred from electronic annotation. Source: InterPro phospholipid-hydroperoxide glutathione peroxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing and alternative initiation. [Align] [Select]

Isoform Mitochondrial (identifier: P36970-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Nuclear (identifier: Q91XR8-1) The sequence of this isoform can be found in the external entry Q91XR8. Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform Cytoplasmic (identifier: P36970-2) The sequence of this isoform differs from the canonical sequence as follows: 1-27: Missing.
Note: Produced by alternative initiation at Met-28 of isoform Mitochondrial.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – ?

Mitochondrion Potential

Chain

? – 197

Phospholipid hydroperoxide glutathione peroxidase, mitochondrial

PRO_0000013073

Sites

Active site

Amino acid modifications

Non-standard residue

Selenocysteine

Natural variations

Alternative sequence

1 – 27

Missing in isoform Cytoplasmic.

VSP_018746

Experimental info

Sequence conflict

A → S in AAC52503. Ref.1

Sequence conflict

A → S in CAD61276. Ref.2

Sequence conflict

A → S in CAD61277. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

Isoform Mitochondrial [UniParc].

Last modified February 26, 2008. Version 3.
Checksum: 83FF001A5A42F814
Show »

FASTA

197

22,225

        10         20         30         40         50         60 
MSWGRLSRLL KPALLCGALA VPGLAGTMCA SRDDWRCARS MHEFAAKDID GHMVCLDKYR 

        70         80         90        100        110        120 
GCVCIVTNVA SQUGKTDVNY TQLVDLHARY AECGLRILAF PCNQFGRQEP GSNQEIKEFA 

       130        140        150        160        170        180 
AGYNVRFDMY SKICVNGDDA HPLWKWMKVQ PKGRGMLGNA IKWNFTKFLI DKNGCVVKRY 

       190 
GPMEEPQVIE KDLPCYL

« Hide

Isoform Nuclear [UniParc].

See Q91XR8.

–

Isoform Cytoplasmic [UniParc].

Checksum: A882EE0827902EA0
Show »

FASTA

170

19,490

References

[1]	"Rat phospholipid-hydroperoxide glutathione peroxidase. cDNA cloning and identification of multiple transcription and translation start sites." Pushpa-Rekha T.R., Burdsall A.L., Oleksa L.M., Chisolm G.M., Driscoll D.M. J. Biol. Chem. 270:26993-26999(1995) [PubMed: 7592947] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Testis.
[2]	"Distinct promoters determine alternative transcription of gpx-4 into PHGPx variants." Maiorino M., Scapin M., Ursini F., Biasolo M., Bosello V., Flohe L. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Wistar.
[3]	"Molecular cloning and functional expression of a cDNA for rat phospholipid hydroperoxide glutathione peroxidase: 3'-untranslated region of the gene is necessary for functional expression." Imai H., Sumi D., Hanamoto A., Arai M., Sugiyama A., Chiba N., Kuchino Y., Nakagawa Y. J. Biochem. 118:1061-1067(1995) [PubMed: 8749327] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-197. Strain: Sprague-Dawley. Tissue: Brain.
[4]	"Rat liver phospholipid hydroperoxide glutathione peroxidase." Sunde R.A., Dyer J.A., Moran T.V., Evenson J.K. Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 21-197. Strain: Sprague-Dawley. Tissue: Liver.
[5]	"Phospholipid hydroperoxide glutathione peroxidase of rat testis. Gonadotropin dependence and immunocytochemical identification." Roveri A., Casasco A., Maiorino M., Dalan P., Calligaro A., Ursini F. J. Biol. Chem. 267:6142-6146(1992) [PubMed: 1556123] [Abstract] Cited for: CHARACTERIZATION. Tissue: Testis.
[6]	"Identification of novel immunodominant epididymal sperm proteins using combinatorial approach." Khan S.A., Suryawanshi A.R., Ranpura S.A., Jadhav S.V., Khole V.V. Reproduction 138:81-93(2009) [PubMed: 19423663] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U37427 mRNA. Translation: AAC52503.2. AJ537598 Genomic DNA. Translation: CAD61276.1. AJ537598 Genomic DNA. Translation: CAD61277.1. X82679 mRNA. Translation: CAA57996.1. L24896 mRNA. Translation: AAA41842.2.
IPI	IPI00326703. IPI00760136.
PIR	JC4332.
RefSeq	NP_001034938.1. NM_001039849.1. NP_058861.3. NM_017165.2.
UniGene	Rn.3647.
3D structure databases
ProteinModelPortal	P36970.
ModBase	Search...
Protein-protein interaction databases
STRING	P36970.
Protein family/group databases
PeroxiBase	3733. RnoGPx04-a. 3812. RnoGPx04-b.
Proteomic databases
PRIDE	P36970.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	29328.
KEGG	rno:29328.
Organism-specific databases
CTD	2879.
RGD	69226. Gpx4.
Phylogenomic databases
eggNOG	roNOG17340.
GeneTree	ENSGT00550000074312.
HOVERGEN	HBG004333.
InParanoid	P36970.
OrthoDB	EOG4K3KXQ.
Enzyme and pathway databases
BRENDA	1.11.1.12. 5301.
Gene expression databases
ArrayExpress	P36970.
Genevestigator	P36970.
Family and domain databases
InterPro	IPR000889. Glutathione_peroxidase. IPR012336. Thioredoxin-like_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
KO	K05361.
PANTHER	PTHR11592. Glut_peroxidase. 1 hit.
Pfam	PF00255. GSHPx. 1 hit. [Graphical view]
PIRSF	PIRSF000303. Glutathion_perox. 1 hit.
PRINTS	PR01011. GLUTPROXDASE.
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS00460. GLUTATHIONE_PEROXID_1. 1 hit. PS00763. GLUTATHIONE_PEROXID_2. 1 hit. PS51355. GLUTATHIONE_PEROXID_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	608793.

Entry information

Entry name

GPX41_RAT

Accession

Primary (citable) accession number: P36970

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	February 26, 2008
Last modified:	November 16, 2011
This is version 102 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents