Phospholipid hydroperoxide glutathione peroxidase, mitochondrial precursor

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Molecule processing

Sites

Amino acid modifications

Natural variations

Molecule processing

Sites

Amino acid modifications

Natural variations

Sequence databases

3D structure databases

Protein family/group databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

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Reviewed, UniProtKB/Swiss-Prot P36968 (GPX4_PIG)

Last modified June 16, 2009. Version 76. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Protein names

Recommended name:
    Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
      Short name=PHGPx
    EC=1.11.1.12
Alternative name(s):
    GPX-4

Gene names

Name:

GPX4

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

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Sequence length	197 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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Function	Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage By similarity.
Catalytic activity	2 glutathione + a lipid hydroperoxide = glutathione disulfide + lipid + 2 H₂O.
Subunit structure	Monomer.
Subcellular location	Mitochondrion. Cytoplasm.
Sequence similarities	Belongs to the glutathione peroxidase family.

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Keywords
Cellular component	Cytoplasm Mitochondrion
Coding sequence diversity	Alternative initiation Selenocysteine
Domain	Transit peptide
Ligand	Selenium
Molecular function	Developmental protein Oxidoreductase Peroxidase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to oxidative stress Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glutathione peroxidase activity Inferred from electronic annotation. Source: InterPro phospholipid-hydroperoxide glutathione peroxidase activity Inferred from electronic annotation. Source: EC selenium binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Transit peptide

1 – ?

Mitochondrion Potential

Chain

? – 197

Phospholipid hydroperoxide glutathione peroxidase, mitochondrial

PRO_0000013071

Active site

Non-standard residue

Selenocysteine

Alternative sequence

1 – 27

Missing in isoform Cytoplasmic.

VSP_018744

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Sequence		Length	Mass (Da)
Isoform Mitochondrial [UniParc]. Last modified February 26, 2008. Version 3. Checksum: 34865B0BEBFA69D0 Show »	FASTA	197	22,337
10 20 30 40 50 60 MSFSRLFRLL KPTLLCGTLA VPGLAGTMCA SRDDWRCARS MHEFSAKDID GHMVNLDKYR 70 80 90 100 110 120 GYVCIVTNVA SQUGKTEVNY TQLVDLHARY AECGLRILAF PCNQFGRQEP GSDAEIKEFA 130 140 150 160 170 180 AGYNVKFDMF SKICVNGDDA HPLWKWMKVQ PKGRGMLGNA IKWNFTKFLI DKNGCVVKRY 190 GPMEEPQVIE KDLPCYL « Hide
Isoform Cytoplasmic. Checksum: EBA2276405A6FACE Show »	FASTA	170	19,491
10 20 30 40 50 60 MCASRDDWRC ARSMHEFSAK DIDGHMVNLD KYRGYVCIVT NVASQUGKTE VNYTQLVDLH 70 80 90 100 110 120 ARYAECGLRI LAFPCNQFGR QEPGSDAEIK EFAAGYNVKF DMFSKICVNG DDAHPLWKWM 130 140 150 160 170 KVQPKGRGML GNAIKWNFTK FLIDKNGCVV KRYGPMEEPQ VIEKDLPCYL « Hide

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[1]	"Phospholipid hydroperoxide glutathione peroxidase: full-length pig blastocyst cDNA sequence and regulation by selenium status." Sunde R.A., Dyer J.A., Moran T., Evenson J.K., Sugimoto M. Biochem. Biophys. Res. Commun. 193:905-911(1993) [PubMed: 8323565] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Phospholipid-hydroperoxide glutathione peroxidase. Genomic DNA, cDNA, and deduced amino acid sequence." Brigelius-Flohe R., Aumann K.-D., Bloecker H., Gross G., Kloeppel K.-D., Maiorino M., Roveri A., Schuckelt R., Ursini F., Wingender E., Flohe L. J. Biol. Chem. 269:7342-7348(1994) [PubMed: 8125951] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Heart and Liver.
[3]	"Phospholipid hydroperoxide glutathione peroxidase is a selenoenzyme distinct from the classical glutathione peroxidase as evident from cDNA and amino acid sequencing." Schuckelt R., Brigelius-Flohe R., Maiorino M., Roveri A., Reumkens J., Strassburger W., Ursini F., Wolf B., Flohe L. Free Radic. Res. Commun. 14:343-361(1991) [PubMed: 1778506] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 40-197, PARTIAL PROTEIN SEQUENCE. Tissue: Heart.

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Sequence databases
	L12743 mRNA. Translation: AAA31099.2. L12743 mRNA. Translation: AAA31098.2. X76009 mRNA. Translation: CAA53596.1. Different initiation. X76008 Genomic DNA. Translation: CAA53595.1. Different initiation. S80257 mRNA. Translation: AAB21327.2.
PIR	JN0608.
RefSeq	NP_999572.1.
UniGene	Ssc.11742
3D structure databases
HSSP	HSSP built from PDB template 1GP1 based on UniProtKB P00435.
SMR	P36968. Positions 36-197.
ModBase	Search...
Protein family/group databases
PeroxiBase	3723. SscGPx04.
Genome annotation databases
GeneID	399537.
KEGG	ssc:399537.
Phylogenomic databases
HOVERGEN	P36968.
Enzyme and pathway databases
BRENDA	1.11.1.12. 249.
Family and domain databases
InterPro	IPR000889. Glutathione_peroxidase. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PANTHER	PTHR11592. Glut_peroxidase. 1 hit.
Pfam	PF00255. GSHPx. 1 hit. [Graphical view]
PIRSF	PIRSF000303. Glutathion_perox. 1 hit.
PRINTS	PR01011. GLUTPROXDASE.
PROSITE	PS00460. GLUTATHIONE_PEROXID_1. 1 hit. PS00763. GLUTATHIONE_PEROXID_2. 1 hit. PS51355. GLUTATHIONE_PEROXID_3. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry name

GPX4_PIG

Accession

Primary (citable) accession number: P36968

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	February 26, 2008
Last modified:	June 16, 2009
This is version 76 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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SIMILARITY comments

Index of protein domains and families

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform Mitochondrial (identifier: P36968-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Cytoplasmic (identifier: P36968-2)

The sequence of this isoform differs from the canonical sequence as follows:
1-27: Missing.