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Protein

Phospholipid hydroperoxide glutathione peroxidase, mitochondrial

Gene

GPX4

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage. Essential for maturation and survival of photoreceptor cells. Plays a role in a primary T cell response to viral and parasitic infection by protecting T cells from ferroptosis, a cell death resulting from an iron-dependent accumulation of lipid reactive oxygen species, and by supporting T cell expansion.By similarity

Catalytic activityi

2 glutathione + a hydroperoxy-fatty-acyl-[lipid] = glutathione disulfide + a hydroxy-fatty-acyl-[lipid] + H2O.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei73 – 7311 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Oxidoreductase, Peroxidase

Protein family/group databases

PeroxiBasei3723. SscGPx04.

Chemistry

SwissLipidsiSLP:000001475.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipid hydroperoxide glutathione peroxidase, mitochondrial (EC:1.11.1.121 Publication)
Short name:
PHGPx
Alternative name(s):
Glutathione peroxidase 4
Short name:
GPx-4
Short name:
GSHPx-4
Gene namesi
Name:GPX4
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

  • Mitochondrion By similarity
  • Cytoplasm By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 197Phospholipid hydroperoxide glutathione peroxidase, mitochondrialPRO_0000013071
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

PaxDbiP36968.
PeptideAtlasiP36968.

Expressioni

Gene expression databases

GenevisibleiP36968. SS.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000021702.

Structurei

3D structure databases

ProteinModelPortaliP36968.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutathione peroxidase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410JKJH. Eukaryota.
ENOG410ZC0K. LUCA.
HOVERGENiHBG004333.
InParanoidiP36968.
KOiK05361.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR000889. Glutathione_peroxidase.
IPR029759. GPX_AS.
IPR029760. GPX_CS.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11592. PTHR11592. 1 hit.
PfamiPF00255. GSHPx. 1 hit.
[Graphical view]
PIRSFiPIRSF000303. Glutathion_perox. 1 hit.
PRINTSiPR01011. GLUTPROXDASE.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS00460. GLUTATHIONE_PEROXID_1. 1 hit.
PS00763. GLUTATHIONE_PEROXID_2. 1 hit.
PS51355. GLUTATHIONE_PEROXID_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Mitochondrial (identifier: P36968-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSFSRLFRLL KPTLLCGTLA VPGLAGTMCA SRDDWRCARS MHEFSAKDID
60 70 80 90 100
GHMVNLDKYR GYVCIVTNVA SQUGKTEVNY TQLVDLHARY AECGLRILAF
110 120 130 140 150
PCNQFGRQEP GSDAEIKEFA AGYNVKFDMF SKICVNGDDA HPLWKWMKVQ
160 170 180 190
PKGRGMLGNA IKWNFTKFLI DKNGCVVKRY GPMEEPQVIE KDLPCYL
Length:197
Mass (Da):22,337
Last modified:February 26, 2008 - v3
Checksum:i34865B0BEBFA69D0
GO
Isoform Cytoplasmic (identifier: P36968-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-27: Missing.

Show »
Length:170
Mass (Da):19,491
Checksum:iEBA2276405A6FACE
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2727Missing in isoform Cytoplasmic. 2 PublicationsVSP_018744Add
BLAST

Non-standard residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-standard residuei73 – 731Selenocysteine1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12743 mRNA. Translation: AAA31098.2.
L12743 mRNA. Translation: AAA31099.2.
X76008 Genomic DNA. Translation: CAA53595.2.
X76009 mRNA. Translation: CAA53596.2.
S80257 mRNA. Translation: AAB21327.2.
PIRiJN0608.
RefSeqiNP_999572.1. NM_214407.1. [P36968-1]
UniGeneiSsc.93915.

Genome annotation databases

GeneIDi399537.
KEGGissc:399537.

Keywords - Coding sequence diversityi

Alternative initiation, Selenocysteine

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12743 mRNA. Translation: AAA31098.2.
L12743 mRNA. Translation: AAA31099.2.
X76008 Genomic DNA. Translation: CAA53595.2.
X76009 mRNA. Translation: CAA53596.2.
S80257 mRNA. Translation: AAB21327.2.
PIRiJN0608.
RefSeqiNP_999572.1. NM_214407.1. [P36968-1]
UniGeneiSsc.93915.

3D structure databases

ProteinModelPortaliP36968.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000021702.

Chemistry

SwissLipidsiSLP:000001475.

Protein family/group databases

PeroxiBasei3723. SscGPx04.

Proteomic databases

PaxDbiP36968.
PeptideAtlasiP36968.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi399537.
KEGGissc:399537.

Organism-specific databases

CTDi2879.

Phylogenomic databases

eggNOGiENOG410JKJH. Eukaryota.
ENOG410ZC0K. LUCA.
HOVERGENiHBG004333.
InParanoidiP36968.
KOiK05361.

Gene expression databases

GenevisibleiP36968. SS.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR000889. Glutathione_peroxidase.
IPR029759. GPX_AS.
IPR029760. GPX_CS.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11592. PTHR11592. 1 hit.
PfamiPF00255. GSHPx. 1 hit.
[Graphical view]
PIRSFiPIRSF000303. Glutathion_perox. 1 hit.
PRINTSiPR01011. GLUTPROXDASE.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS00460. GLUTATHIONE_PEROXID_1. 1 hit.
PS00763. GLUTATHIONE_PEROXID_2. 1 hit.
PS51355. GLUTATHIONE_PEROXID_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Phospholipid hydroperoxide glutathione peroxidase: full-length pig blastocyst cDNA sequence and regulation by selenium status."
    Sunde R.A., Dyer J.A., Moran T., Evenson J.K., Sugimoto M.
    Biochem. Biophys. Res. Commun. 193:905-911(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS MITOCHONDRIAL AND CYTOPLASMIC).
  2. "Phospholipid-hydroperoxide glutathione peroxidase. Genomic DNA, cDNA, and deduced amino acid sequence."
    Brigelius-Flohe R., Aumann K.-D., Bloecker H., Gross G., Kloeppel K.-D., Maiorino M., Roveri A., Schuckelt R., Ursini F., Wingender E., Flohe L.
    J. Biol. Chem. 269:7342-7348(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM CYTOPLASMIC), PARTIAL PROTEIN SEQUENCE.
    Tissue: Heart and Liver.
  3. Flohe L.
    Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 73.
  4. "Phospholipid hydroperoxide glutathione peroxidase is a selenoenzyme distinct from the classical glutathione peroxidase as evident from cDNA and amino acid sequencing."
    Schuckelt R., Brigelius-Flohe R., Maiorino M., Roveri A., Reumkens J., Strassburger W., Ursini F., Wolf B., Flohe L.
    Free Radic. Res. Commun. 14:343-361(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 40-197 (ISOFORM MITOCHONDRIAL), PARTIAL PROTEIN SEQUENCE.
    Tissue: Heart.
  5. "The selenoenzyme phospholipid hydroperoxide glutathione peroxidase."
    Ursini F., Maiorino M., Gregolin C.
    Biochim. Biophys. Acta 839:62-70(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, SUBUNIT, SELENOCYSTEINE.
    Tissue: Heart.

Entry informationi

Entry nameiGPX4_PIG
AccessioniPrimary (citable) accession number: P36968
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: February 26, 2008
Last modified: July 6, 2016
This is version 112 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

During purification the protein has been found to contain 1 atom of selenium per monomer.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.