P36954 (RPB9_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase II subunit RPB9
Short name=RNA polymerase II subunit B9

Alternative name(s):
DNA-directed RNA polymerase II subunit I
RNA polymerase II 14.5 kDa subunit
Short name=RPB14.5

Gene names

Name:

POLR2I

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	125 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB9 is part of the upper jaw surrounding the central large cleft and thought to grab the incoming DNA template By similarity. Ref.5
Subunit structure	Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. Ref.5
Subcellular location	Nucleus Ref.5.
Sequence similarities	Belongs to the archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family. Contains 1 TFIIS-type zinc finger.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	7-methylguanosine mRNA capping Traceable author statement. Source: Reactome mRNA splicing, via spliceosome Traceable author statement. Source: Reactome positive regulation of viral transcription Traceable author statement. Source: Reactome protein phosphorylation Inferred from direct assay Ref.5. Source: GOC transcription elongation from RNA polymerase II promoter Traceable author statement. Source: Reactome transcription initiation from RNA polymerase II promoter Traceable author statement. Source: Reactome transcription-coupled nucleotide-excision repair Traceable author statement. Source: Reactome viral reproduction Traceable author statement. Source: Reactome
Cellular_component	DNA-directed RNA polymerase II, core complex Inferred from direct assay Ref.5. Source: UniProtKB
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Traceable author statement Ref.1. Source: ProtInc zinc ion binding Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 125	125	DNA-directed RNA polymerase II subunit RPB9		PRO_0000121467
Regions
	Zinc finger	17 – 42	26	C4-type Potential
	Zinc finger	82 – 124	43	TFIIS-type
Sites
	Metal binding	17	1	Zinc 1 By similarity
	Metal binding	20	1	Zinc 1 By similarity
	Metal binding	39	1	Zinc 1 By similarity
	Metal binding	42	1	Zinc 1 By similarity
	Metal binding	86	1	Zinc 2 By similarity
	Metal binding	89	1	Zinc 2 By similarity
	Metal binding	114	1	Zinc 2 By similarity
	Metal binding	119	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P36954 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 6520687BB57EE018	FASTA	125	14,523
	10 20 30 40 50 60 MEPDGTYEPG FVGIRFCQEC NNMLYPKEDK ENRILLYACR NCDYQQEADN SCIYVNKITH 70 80 90 100 110 120 EVDELTQIIA DVSQDPTLPR TEDHPCQKCG HKEAVFFQSH SARAEDAMRL YYVCTAPHCG HRWTE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of the gene encoding the 14.5 kDa subunit of human RNA polymerase II." Acker J., Wintzerith M., Vigneron M., Kedinger C. Nucleic Acids Res. 21:5345-5350(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Uterus.
[5]	"Immunoaffinity purification and functional characterization of human transcription factor IIH and RNA polymerase II from clonal cell lines that conditionally express epitope-tagged subunits of the multiprotein complexes." Kershnar E., Wu S.-Y., Chiang C.-M. J. Biol. Chem. 273:34444-34453(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE RNA POLYMERASE II CORE-COMPLEX, SUBCELLULAR LOCATION.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z23102 Genomic DNA. Translation: CAA80649.1. AK312206 mRNA. Translation: BAG35139.1. AD001527 Genomic DNA. Translation: AAB51181.1. BC017112 mRNA. Translation: AAH17112.1. BC067794 mRNA. Translation: AAH67794.1.
IPI	IPI00006113.
PIR	S41621.
RefSeq	NP_006224.1. NM_006233.4.
UniGene	Hs.47062.
3D structure databases
ProteinModelPortal	P36954.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-32917N.
IntAct	P36954. 6 interactions.
STRING	9606.ENSP00000221859.
PTM databases
PhosphoSite	P36954.
Polymorphism databases
DMDM	548702.
Proteomic databases
PaxDb	P36954.
PeptideAtlas	P36954.
PRIDE	P36954.
Protocols and materials databases
DNASU	5438.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000221859; ENSP00000221859; ENSG00000105258.
GeneID	5438.
KEGG	hsa:5438.
UCSC	uc002ode.3. human.
Organism-specific databases
CTD	5438.
GeneCards	GC19M036604.
HGNC	HGNC:9196. POLR2I.
HPA	HPA045156.
MIM	180662. gene.
neXtProt	NX_P36954.
PharmGKB	PA33516.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1594.
HOGENOM	HOG000228134.
HOVERGEN	HBG009981.
InParanoid	P36954.
KO	K03017.
OMA	MHEIDEL.
OrthoDB	EOG4XPQH7.
PhylomeDB	P36954.
Enzyme and pathway databases
Reactome	REACT_116125. Disease. REACT_1675. mRNA Processing. REACT_1788. Transcription. REACT_1892. Elongation arrest and recovery. REACT_216. DNA Repair. REACT_71. Gene Expression.
Gene expression databases
Bgee	P36954.
CleanEx	HS_POLR2I.
Genevestigator	P36954.
GermOnline	ENSG00000105258. Homo sapiens.
Family and domain databases
InterPro	IPR019761. DNA-dir_RNA_pol-M_15_CS. IPR001529. DNA-dir_RNA_pol_M/15kDasu. IPR012164. DNA-dir_RNApol_C11. IPR001222. Znf_TFIIS. [Graphical view]
Pfam	PF02150. RNA_POL_M_15KD. 1 hit. PF01096. TFIIS_C. 1 hit. [Graphical view]
PIRSF	PIRSF005586. RNApol_RpoM. 1 hit.
SMART	SM00661. RPOL9. 1 hit. SM00440. ZnF_C2C2. 1 hit. [Graphical view]
PROSITE	PS01030. RNA_POL_M_15KD. 1 hit. PS00466. ZF_TFIIS_1. 1 hit. PS51133. ZF_TFIIS_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	POLR2I. human.
GenomeRNAi	5438.
NextBio	21041.
PMAP-CutDB	P36954.
SOURCE	Search...

Entry information

Entry name

RPB9_HUMAN

Accession

Primary (citable) accession number: P36954
Secondary accession number(s): B2R5J2, Q6NW05

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 1, 2013
This is version 132 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.