Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P36954

- RPB9_HUMAN

UniProt

P36954 - RPB9_HUMAN

Protein

DNA-directed RNA polymerase II subunit RPB9

Gene

POLR2I

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB9 is part of the upper jaw surrounding the central large cleft and thought to grab the incoming DNA template By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi17 – 171Zinc 1By similarity
    Metal bindingi20 – 201Zinc 1By similarity
    Metal bindingi39 – 391Zinc 1By similarity
    Metal bindingi42 – 421Zinc 1By similarity
    Metal bindingi86 – 861Zinc 2By similarity
    Metal bindingi89 – 891Zinc 2By similarity
    Metal bindingi114 – 1141Zinc 2By similarity
    Metal bindingi119 – 1191Zinc 2By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri17 – 4226C4-typeSequence AnalysisAdd
    BLAST
    Zinc fingeri82 – 12443TFIIS-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. DNA-directed RNA polymerase activity Source: ProtInc
    3. zinc ion binding Source: ProtInc

    GO - Biological processi

    1. 7-methylguanosine mRNA capping Source: Reactome
    2. DNA repair Source: Reactome
    3. gene expression Source: Reactome
    4. maintenance of transcriptional fidelity during DNA-templated transcription elongation from RNA polymerase II promoter Source: RefGenome
    5. mRNA splicing, via spliceosome Source: Reactome
    6. nucleotide-excision repair Source: Reactome
    7. positive regulation of viral transcription Source: Reactome
    8. RNA splicing Source: Reactome
    9. transcription-coupled nucleotide-excision repair Source: RefGenome
    10. transcription elongation from RNA polymerase II promoter Source: Reactome
    11. transcription from RNA polymerase II promoter Source: UniProtKB
    12. transcription initiation from RNA polymerase II promoter Source: RefGenome
    13. viral process Source: Reactome

    Keywords - Biological processi

    Transcription

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_12417. MicroRNA (miRNA) biogenesis.
    REACT_125. Processing of Capped Intron-Containing Pre-mRNA.
    REACT_1470. mRNA Capping.
    REACT_1628. Transcription-coupled NER (TC-NER).
    REACT_1655. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
    REACT_1753. mRNA Splicing - Minor Pathway.
    REACT_1851. RNA Polymerase II Transcription Initiation.
    REACT_1941. Formation of transcription-coupled NER (TC-NER) repair complex.
    REACT_2089. RNA Polymerase II Promoter Escape.
    REACT_22107. RNA Polymerase II Pre-transcription Events.
    REACT_22201. Formation of HIV elongation complex in the absence of HIV Tat.
    REACT_2222. Dual incision reaction in TC-NER.
    REACT_467. mRNA Splicing - Major Pathway.
    REACT_6143. Pausing and recovery of Tat-mediated HIV elongation.
    REACT_6162. Tat-mediated elongation of the HIV-1 transcript.
    REACT_6233. Transcription of the HIV genome.
    REACT_6237. RNA Pol II CTD phosphorylation and interaction with CE.
    REACT_6244. Pausing and recovery of HIV elongation.
    REACT_6253. RNA Polymerase II HIV Promoter Escape.
    REACT_6259. HIV elongation arrest and recovery.
    REACT_6261. Abortive elongation of HIV-1 transcript in the absence of Tat.
    REACT_6319. Formation of the HIV-1 Early Elongation Complex.
    REACT_6332. HIV Transcription Initiation.
    REACT_6344. Tat-mediated HIV elongation arrest and recovery.
    REACT_6346. Formation of HIV-1 elongation complex containing HIV-1 Tat.
    REACT_6354. Viral Messenger RNA Synthesis.
    REACT_833. RNA Polymerase II Transcription Elongation.
    REACT_834. RNA Polymerase II Transcription Initiation And Promoter Clearance.
    REACT_846. Formation of the Early Elongation Complex.
    REACT_975. RNA Pol II CTD phosphorylation and interaction with CE.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-directed RNA polymerase II subunit RPB9
    Short name:
    RNA polymerase II subunit B9
    Alternative name(s):
    DNA-directed RNA polymerase II subunit I
    RNA polymerase II 14.5 kDa subunit
    Short name:
    RPB14.5
    Gene namesi
    Name:POLR2I
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:9196. POLR2I.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. DNA-directed RNA polymerase II, core complex Source: UniProtKB
    2. nucleoplasm Source: Reactome
    3. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    DNA-directed RNA polymerase, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA33516.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 125125DNA-directed RNA polymerase II subunit RPB9PRO_0000121467Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP36954.
    PaxDbiP36954.
    PeptideAtlasiP36954.
    PRIDEiP36954.

    PTM databases

    PhosphoSiteiP36954.

    Miscellaneous databases

    PMAP-CutDBP36954.

    Expressioni

    Gene expression databases

    ArrayExpressiP36954.
    BgeeiP36954.
    CleanExiHS_POLR2I.
    GenevestigatoriP36954.

    Organism-specific databases

    HPAiHPA045156.

    Interactioni

    Subunit structurei

    Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.1 Publication

    Protein-protein interaction databases

    BioGridi111434. 20 interactions.
    DIPiDIP-32917N.
    IntActiP36954. 6 interactions.
    STRINGi9606.ENSP00000221859.

    Structurei

    3D structure databases

    ProteinModelPortaliP36954.
    SMRiP36954. Positions 15-125.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Contains 1 TFIIS-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri17 – 4226C4-typeSequence AnalysisAdd
    BLAST
    Zinc fingeri82 – 12443TFIIS-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG1594.
    HOGENOMiHOG000228134.
    HOVERGENiHBG009981.
    InParanoidiP36954.
    KOiK03017.
    OMAiMHEIDEL.
    OrthoDBiEOG7BW0M6.
    PhylomeDBiP36954.
    TreeFamiTF103032.

    Family and domain databases

    InterProiIPR019761. DNA-dir_RNA_pol-M_15_CS.
    IPR001529. DNA-dir_RNA_pol_M/15kDasu.
    IPR012164. DNA-dir_RNApol_C11.
    IPR001222. Znf_TFIIS.
    [Graphical view]
    PfamiPF02150. RNA_POL_M_15KD. 1 hit.
    PF01096. TFIIS_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005586. RNApol_RpoM. 1 hit.
    SMARTiSM00661. RPOL9. 1 hit.
    SM00440. ZnF_C2C2. 1 hit.
    [Graphical view]
    PROSITEiPS01030. RNA_POL_M_15KD. 1 hit.
    PS00466. ZF_TFIIS_1. 1 hit.
    PS51133. ZF_TFIIS_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P36954-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEPDGTYEPG FVGIRFCQEC NNMLYPKEDK ENRILLYACR NCDYQQEADN    50
    SCIYVNKITH EVDELTQIIA DVSQDPTLPR TEDHPCQKCG HKEAVFFQSH 100
    SARAEDAMRL YYVCTAPHCG HRWTE 125
    Length:125
    Mass (Da):14,523
    Last modified:June 1, 1994 - v1
    Checksum:i6520687BB57EE018
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z23102 Genomic DNA. Translation: CAA80649.1.
    AK312206 mRNA. Translation: BAG35139.1.
    AD001527 Genomic DNA. Translation: AAB51181.1.
    BC017112 mRNA. Translation: AAH17112.1.
    BC067794 mRNA. Translation: AAH67794.1.
    CCDSiCCDS12487.1.
    PIRiS41621.
    RefSeqiNP_006224.1. NM_006233.4.
    UniGeneiHs.47062.

    Genome annotation databases

    EnsembliENST00000221859; ENSP00000221859; ENSG00000105258.
    GeneIDi5438.
    KEGGihsa:5438.
    UCSCiuc002ode.3. human.

    Polymorphism databases

    DMDMi548702.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z23102 Genomic DNA. Translation: CAA80649.1 .
    AK312206 mRNA. Translation: BAG35139.1 .
    AD001527 Genomic DNA. Translation: AAB51181.1 .
    BC017112 mRNA. Translation: AAH17112.1 .
    BC067794 mRNA. Translation: AAH67794.1 .
    CCDSi CCDS12487.1.
    PIRi S41621.
    RefSeqi NP_006224.1. NM_006233.4.
    UniGenei Hs.47062.

    3D structure databases

    ProteinModelPortali P36954.
    SMRi P36954. Positions 15-125.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111434. 20 interactions.
    DIPi DIP-32917N.
    IntActi P36954. 6 interactions.
    STRINGi 9606.ENSP00000221859.

    PTM databases

    PhosphoSitei P36954.

    Polymorphism databases

    DMDMi 548702.

    Proteomic databases

    MaxQBi P36954.
    PaxDbi P36954.
    PeptideAtlasi P36954.
    PRIDEi P36954.

    Protocols and materials databases

    DNASUi 5438.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000221859 ; ENSP00000221859 ; ENSG00000105258 .
    GeneIDi 5438.
    KEGGi hsa:5438.
    UCSCi uc002ode.3. human.

    Organism-specific databases

    CTDi 5438.
    GeneCardsi GC19M036604.
    HGNCi HGNC:9196. POLR2I.
    HPAi HPA045156.
    MIMi 180662. gene.
    neXtProti NX_P36954.
    PharmGKBi PA33516.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1594.
    HOGENOMi HOG000228134.
    HOVERGENi HBG009981.
    InParanoidi P36954.
    KOi K03017.
    OMAi MHEIDEL.
    OrthoDBi EOG7BW0M6.
    PhylomeDBi P36954.
    TreeFami TF103032.

    Enzyme and pathway databases

    Reactomei REACT_12417. MicroRNA (miRNA) biogenesis.
    REACT_125. Processing of Capped Intron-Containing Pre-mRNA.
    REACT_1470. mRNA Capping.
    REACT_1628. Transcription-coupled NER (TC-NER).
    REACT_1655. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
    REACT_1753. mRNA Splicing - Minor Pathway.
    REACT_1851. RNA Polymerase II Transcription Initiation.
    REACT_1941. Formation of transcription-coupled NER (TC-NER) repair complex.
    REACT_2089. RNA Polymerase II Promoter Escape.
    REACT_22107. RNA Polymerase II Pre-transcription Events.
    REACT_22201. Formation of HIV elongation complex in the absence of HIV Tat.
    REACT_2222. Dual incision reaction in TC-NER.
    REACT_467. mRNA Splicing - Major Pathway.
    REACT_6143. Pausing and recovery of Tat-mediated HIV elongation.
    REACT_6162. Tat-mediated elongation of the HIV-1 transcript.
    REACT_6233. Transcription of the HIV genome.
    REACT_6237. RNA Pol II CTD phosphorylation and interaction with CE.
    REACT_6244. Pausing and recovery of HIV elongation.
    REACT_6253. RNA Polymerase II HIV Promoter Escape.
    REACT_6259. HIV elongation arrest and recovery.
    REACT_6261. Abortive elongation of HIV-1 transcript in the absence of Tat.
    REACT_6319. Formation of the HIV-1 Early Elongation Complex.
    REACT_6332. HIV Transcription Initiation.
    REACT_6344. Tat-mediated HIV elongation arrest and recovery.
    REACT_6346. Formation of HIV-1 elongation complex containing HIV-1 Tat.
    REACT_6354. Viral Messenger RNA Synthesis.
    REACT_833. RNA Polymerase II Transcription Elongation.
    REACT_834. RNA Polymerase II Transcription Initiation And Promoter Clearance.
    REACT_846. Formation of the Early Elongation Complex.
    REACT_975. RNA Pol II CTD phosphorylation and interaction with CE.

    Miscellaneous databases

    ChiTaRSi POLR2I. human.
    GeneWikii POLR2I.
    GenomeRNAii 5438.
    NextBioi 21041.
    PMAP-CutDB P36954.
    PROi P36954.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P36954.
    Bgeei P36954.
    CleanExi HS_POLR2I.
    Genevestigatori P36954.

    Family and domain databases

    InterProi IPR019761. DNA-dir_RNA_pol-M_15_CS.
    IPR001529. DNA-dir_RNA_pol_M/15kDasu.
    IPR012164. DNA-dir_RNApol_C11.
    IPR001222. Znf_TFIIS.
    [Graphical view ]
    Pfami PF02150. RNA_POL_M_15KD. 1 hit.
    PF01096. TFIIS_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005586. RNApol_RpoM. 1 hit.
    SMARTi SM00661. RPOL9. 1 hit.
    SM00440. ZnF_C2C2. 1 hit.
    [Graphical view ]
    PROSITEi PS01030. RNA_POL_M_15KD. 1 hit.
    PS00466. ZF_TFIIS_1. 1 hit.
    PS51133. ZF_TFIIS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure of the gene encoding the 14.5 kDa subunit of human RNA polymerase II."
      Acker J., Wintzerith M., Vigneron M., Kedinger C.
      Nucleic Acids Res. 21:5345-5350(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain and Uterus.
    5. "Immunoaffinity purification and functional characterization of human transcription factor IIH and RNA polymerase II from clonal cell lines that conditionally express epitope-tagged subunits of the multiprotein complexes."
      Kershnar E., Wu S.-Y., Chiang C.-M.
      J. Biol. Chem. 273:34444-34453(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE RNA POLYMERASE II CORE-COMPLEX, SUBCELLULAR LOCATION.
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRPB9_HUMAN
    AccessioniPrimary (citable) accession number: P36954
    Secondary accession number(s): B2R5J2, Q6NW05
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 145 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3