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Protein

Afamin

Gene

Afm

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Vitamin E binding protein. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the regulation and transport of vitamin E at the blood-brain barrier (By similarity).By similarity

GO - Molecular functioni

  1. vitamin E binding Source: UniProtKB

GO - Biological processi

  1. vitamin transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Afamin
Alternative name(s):
Alpha-albumin
Short name:
Alpha-Alb
Gene namesi
Name:Afm
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi628614. Afm.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121By similarityAdd
BLAST
Chaini22 – 608587AfaminPRO_0000001108Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi33 – 331N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi77 ↔ 86PROSITE-ProRule annotation
Disulfide bondi99 ↔ 114PROSITE-ProRule annotation
Glycosylationi109 – 1091N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi113 ↔ 124PROSITE-ProRule annotation
Disulfide bondi148 ↔ 193PROSITE-ProRule annotation
Glycosylationi153 – 1531N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi224 ↔ 270PROSITE-ProRule annotation
Disulfide bondi269 ↔ 277PROSITE-ProRule annotation
Disulfide bondi289 ↔ 303PROSITE-ProRule annotation
Disulfide bondi302 ↔ 313PROSITE-ProRule annotation
Disulfide bondi340 ↔ 385PROSITE-ProRule annotation
Disulfide bondi384 ↔ 393PROSITE-ProRule annotation
Glycosylationi402 – 4021N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi416 ↔ 462PROSITE-ProRule annotation
Disulfide bondi461 ↔ 470PROSITE-ProRule annotation
Disulfide bondi483 ↔ 499PROSITE-ProRule annotation
Glycosylationi488 – 4881N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi498 ↔ 509PROSITE-ProRule annotation
Disulfide bondi580 ↔ 589PROSITE-ProRule annotation

Post-translational modificationi

N-glycosylated; more than 90% of the glycans are sialylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP36953.
PRIDEiP36953.

PTM databases

PhosphoSiteiP36953.

Expressioni

Gene expression databases

GenevestigatoriP36953.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000057275.

Structurei

3D structure databases

ProteinModelPortaliP36953.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 210189Albumin 1PROSITE-ProRule annotationAdd
BLAST
Domaini211 – 403193Albumin 2PROSITE-ProRule annotationAdd
BLAST
Domaini404 – 599196Albumin 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ALB/AFP/VDB family.PROSITE-ProRule annotation
Contains 3 albumin domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG47806.
HOGENOMiHOG000293137.
HOVERGENiHBG004207.
InParanoidiP36953.
PhylomeDBiP36953.

Family and domain databases

InterProiIPR000264. ALB/AFP/VDB.
IPR020858. Serum_albumin-like.
IPR021177. Serum_albumin/AFP/Afamin.
IPR020857. Serum_albumin_CS.
IPR014760. Serum_albumin_N.
[Graphical view]
PfamiPF00273. Serum_albumin. 3 hits.
[Graphical view]
PIRSFiPIRSF002520. Serum_albumin_subgroup. 1 hit.
PRINTSiPR00802. SERUMALBUMIN.
SMARTiSM00103. ALBUMIN. 3 hits.
[Graphical view]
SUPFAMiSSF48552. SSF48552. 3 hits.
PROSITEiPS00212. ALBUMIN_1. 1 hit.
PS51438. ALBUMIN_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36953-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRHLKLTGFI FFLLSLTESL ALPTKPQDVD HFNATQKFIN ENVAYLTIIA
60 70 80 90 100
SAQYVQEASF EEVEMLVKVM LDYKDRCLAD STLPECSKIA NDAIQDMLCD
110 120 130 140 150
MKGLPQKHNF SHCCRQAGFQ RRLCFFYNKK ANVGFLPPFP TLDPEEKCQA
160 170 180 190 200
YKNNSESFLN LYMYEVARRN PFAFAPVLLN VAARFEEAAT TCCEQQQKAT
210 220 230 240 250
YFQDKAAPIT QYLKALSSYQ RNVCGALLKF GPKTLNSINI AVFSKKFPKI
260 270 280 290 300
GFEDLTSLLE DVSSMYDGCC EGDVVQCIRS QSQVMHHICS KQDSISSKIK
310 320 330 340 350
ACCEKKLPER ADCIINANKD DRPEDLSLRT PKFTDSENVC QERDSEQDKF
360 370 380 390 400
FAEFLYDYSR RHTELSTPEL LRITKVYKDL LEDCCNRKNP LSCYRHAEDK
410 420 430 440 450
FNETTERSLA MVQQECKQFQ ELGKDALQRH FLVKFTKAAP QLPMEELVSL
460 470 480 490 500
SKEMVAALAT CCTLSDEFAC VDNLADLVLG ELCGMNKNRT INPTVDHCCR
510 520 530 540 550
ADFAFRRPCF EHLKADTTYA LPSVSALVSA LRADWCQPLK EDLQNKRHRF
560 570 580 590 600
LVNLVKWMPE ITDEERLCLF TKFTAAGEEC GNIQKPEACF SPESSKTGDV

SQDAEKQR
Length:608
Mass (Da):69,335
Last modified:June 1, 1994 - v1
Checksum:iF33151A6E68A07F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76456 Genomic DNA. Translation: CAA53994.1.
PIRiA53195.
RefSeqiNP_758823.1. NM_172320.1.
UniGeneiRn.214035.

Genome annotation databases

GeneIDi282708.
KEGGirno:282708.
UCSCiRGD:628614. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76456 Genomic DNA. Translation: CAA53994.1.
PIRiA53195.
RefSeqiNP_758823.1. NM_172320.1.
UniGeneiRn.214035.

3D structure databases

ProteinModelPortaliP36953.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000057275.

PTM databases

PhosphoSiteiP36953.

Proteomic databases

PaxDbiP36953.
PRIDEiP36953.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi282708.
KEGGirno:282708.
UCSCiRGD:628614. rat.

Organism-specific databases

CTDi173.
RGDi628614. Afm.

Phylogenomic databases

eggNOGiNOG47806.
HOGENOMiHOG000293137.
HOVERGENiHBG004207.
InParanoidiP36953.
PhylomeDBiP36953.

Miscellaneous databases

NextBioi624841.
PROiP36953.

Gene expression databases

GenevestigatoriP36953.

Family and domain databases

InterProiIPR000264. ALB/AFP/VDB.
IPR020858. Serum_albumin-like.
IPR021177. Serum_albumin/AFP/Afamin.
IPR020857. Serum_albumin_CS.
IPR014760. Serum_albumin_N.
[Graphical view]
PfamiPF00273. Serum_albumin. 3 hits.
[Graphical view]
PIRSFiPIRSF002520. Serum_albumin_subgroup. 1 hit.
PRINTSiPR00802. SERUMALBUMIN.
SMARTiSM00103. ALBUMIN. 3 hits.
[Graphical view]
SUPFAMiSSF48552. SSF48552. 3 hits.
PROSITEiPS00212. ALBUMIN_1. 1 hit.
PS51438. ALBUMIN_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "New albumin gene 3' adjacent to the alpha 1-fetoprotein locus."
    Belanger L., Roy S., Allard D.
    J. Biol. Chem. 269:5481-5484(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sprague-Dawley.

Entry informationi

Entry nameiAFAM_RAT
AccessioniPrimary (citable) accession number: P36953
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 1, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.