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P36953 (AFAM_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Afamin
Alternative name(s):
Alpha-albumin
Short name=Alpha-Alb
Gene names
Name:Afm
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length608 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Vitamin E binding protein. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the regulation and transport of vitamin E at the blood-brain barrier By similarity.

Subcellular location

Secreted.

Post-translational modification

N-glycosylated; more than 90% of the glycans are sialylated By similarity.

Sequence similarities

Belongs to the ALB/AFP/VDB family.

Contains 3 albumin domains.

Ontologies

Keywords
   Biological processTransport
   Cellular componentSecreted
   DomainRepeat
Signal
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processvitamin transport

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentextracellular space

Inferred from direct assay Ref.1. Source: RGD

   Molecular_functionvitamin E binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 By similarity
Chain22 – 608587Afamin
PRO_0000001108

Regions

Domain22 – 210189Albumin 1
Domain211 – 403193Albumin 2
Domain404 – 599196Albumin 3

Amino acid modifications

Glycosylation331N-linked (GlcNAc...) Potential
Glycosylation1091N-linked (GlcNAc...) Potential
Glycosylation1531N-linked (GlcNAc...) Potential
Glycosylation4021N-linked (GlcNAc...) Potential
Glycosylation4881N-linked (GlcNAc...) Potential
Disulfide bond77 ↔ 86 By similarity
Disulfide bond99 ↔ 114 By similarity
Disulfide bond113 ↔ 124 By similarity
Disulfide bond148 ↔ 193 By similarity
Disulfide bond224 ↔ 270 By similarity
Disulfide bond269 ↔ 277 By similarity
Disulfide bond289 ↔ 303 By similarity
Disulfide bond302 ↔ 313 By similarity
Disulfide bond340 ↔ 385 By similarity
Disulfide bond384 ↔ 393 By similarity
Disulfide bond416 ↔ 462 By similarity
Disulfide bond461 ↔ 470 By similarity
Disulfide bond483 ↔ 499 By similarity
Disulfide bond498 ↔ 509 By similarity
Disulfide bond580 ↔ 589 By similarity

Sequences

Sequence LengthMass (Da)Tools
P36953 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: F33151A6E68A07F6

FASTA60869,335
        10         20         30         40         50         60 
MRHLKLTGFI FFLLSLTESL ALPTKPQDVD HFNATQKFIN ENVAYLTIIA SAQYVQEASF 

        70         80         90        100        110        120 
EEVEMLVKVM LDYKDRCLAD STLPECSKIA NDAIQDMLCD MKGLPQKHNF SHCCRQAGFQ 

       130        140        150        160        170        180 
RRLCFFYNKK ANVGFLPPFP TLDPEEKCQA YKNNSESFLN LYMYEVARRN PFAFAPVLLN 

       190        200        210        220        230        240 
VAARFEEAAT TCCEQQQKAT YFQDKAAPIT QYLKALSSYQ RNVCGALLKF GPKTLNSINI 

       250        260        270        280        290        300 
AVFSKKFPKI GFEDLTSLLE DVSSMYDGCC EGDVVQCIRS QSQVMHHICS KQDSISSKIK 

       310        320        330        340        350        360 
ACCEKKLPER ADCIINANKD DRPEDLSLRT PKFTDSENVC QERDSEQDKF FAEFLYDYSR 

       370        380        390        400        410        420 
RHTELSTPEL LRITKVYKDL LEDCCNRKNP LSCYRHAEDK FNETTERSLA MVQQECKQFQ 

       430        440        450        460        470        480 
ELGKDALQRH FLVKFTKAAP QLPMEELVSL SKEMVAALAT CCTLSDEFAC VDNLADLVLG 

       490        500        510        520        530        540 
ELCGMNKNRT INPTVDHCCR ADFAFRRPCF EHLKADTTYA LPSVSALVSA LRADWCQPLK 

       550        560        570        580        590        600 
EDLQNKRHRF LVNLVKWMPE ITDEERLCLF TKFTAAGEEC GNIQKPEACF SPESSKTGDV 


SQDAEKQR

« Hide

References

[1]"New albumin gene 3' adjacent to the alpha 1-fetoprotein locus."
Belanger L., Roy S., Allard D.
J. Biol. Chem. 269:5481-5484(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Sprague-Dawley.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X76456 Genomic DNA. Translation: CAA53994.1.
IPIIPI00207668.
PIRA53195.
RefSeqNP_758823.1. NM_172320.1.
UniGeneRn.214035.

3D structure databases

ProteinModelPortalP36953.
ModBaseSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000057275.

PTM databases

PhosphoSiteP36953.

Proteomic databases

PaxDbP36953.
PRIDEP36953.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID282708.
KEGGrno:282708.
UCSCRGD:628614. rat.

Organism-specific databases

CTD173.
RGD628614. Afm.

Phylogenomic databases

eggNOGNOG47806.
HOGENOMHOG000293137.
HOVERGENHBG004207.
InParanoidP36953.
OrthoDBEOG46MBJ5.

Gene expression databases

ArrayExpressP36953.
GenevestigatorP36953.
GermOnlineENSRNOG00000002878. Rattus norvegicus.

Family and domain databases

InterProIPR000264. ALB/AFP/VDB.
IPR020858. Serum_albumin-like.
IPR021177. Serum_albumin/AFP.
IPR020857. Serum_albumin_CS.
IPR014760. Serum_albumin_N.
[Graphical view]
PfamPF00273. Serum_albumin. 3 hits.
[Graphical view]
PIRSFPIRSF002520. Serum_albumin_subgroup. 1 hit.
PRINTSPR00802. SERUMALBUMIN.
SMARTSM00103. ALBUMIN. 3 hits.
[Graphical view]
SUPFAMSSF48552. Serum_albumin. 3 hits.
PROSITEPS00212. ALBUMIN_1. 1 hit.
PS51438. ALBUMIN_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio624841.

Entry information

Entry nameAFAM_RAT
AccessionPrimary (citable) accession number: P36953
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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