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Protein

Afamin

Gene

Afm

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Functions as carrier for hydrophobic molecules in body fluids. Essential for the solubility and activity of lipidated Wnt family members, including WNT1, WNT2B, WNT3, WNT3A, WNT5A, WNT7A, WNT7B, WNT8, WNT9A, WNT9B, WNT10A and WNT10B. Binds vitamin E. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the transport of vitamin E across the blood-brain barrier.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Afamin
Alternative name(s):
Alpha-albumin
Short name:
Alpha-Alb
Gene namesi
Name:Afm
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi

Organism-specific databases

RGDi628614 Afm

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21By similarityAdd BLAST21
ChainiPRO_000000110822 – 608AfaminAdd BLAST587

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi33N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi77 ↔ 86PROSITE-ProRule annotation
Disulfide bondi99 ↔ 114PROSITE-ProRule annotation
Glycosylationi109N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi113 ↔ 124PROSITE-ProRule annotation
Disulfide bondi148 ↔ 193PROSITE-ProRule annotation
Glycosylationi153N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi224 ↔ 270PROSITE-ProRule annotation
Disulfide bondi269 ↔ 277PROSITE-ProRule annotation
Disulfide bondi289 ↔ 303PROSITE-ProRule annotation
Disulfide bondi302 ↔ 313PROSITE-ProRule annotation
Disulfide bondi340 ↔ 385PROSITE-ProRule annotation
Disulfide bondi384 ↔ 393PROSITE-ProRule annotation
Glycosylationi402N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi416 ↔ 462PROSITE-ProRule annotation
Disulfide bondi461 ↔ 470PROSITE-ProRule annotation
Disulfide bondi483 ↔ 499PROSITE-ProRule annotation
Glycosylationi488N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi498 ↔ 509PROSITE-ProRule annotation
Disulfide bondi580 ↔ 589PROSITE-ProRule annotation

Post-translational modificationi

N-glycosylated; more than 90% of the glycans are sialylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP36953
PRIDEiP36953

PTM databases

iPTMnetiP36953
PhosphoSitePlusiP36953

Interactioni

Subunit structurei

Forms a 1:1 complex with Wnt family members; interacts with WNT1, WNT2B, WNT3, WNT3A, WNT5A, WNT7A, WNT7B, WNT8, WNT9A, WNT9B, WNT10A and WNT10B.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000057275

Structurei

3D structure databases

ProteinModelPortaliP36953
SMRiP36953
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 210Albumin 1PROSITE-ProRule annotationAdd BLAST189
Domaini211 – 403Albumin 2PROSITE-ProRule annotationAdd BLAST193
Domaini404 – 599Albumin 3PROSITE-ProRule annotationAdd BLAST196

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni215 – 319Binding pocket for hydrophobic ligandsBy similarityAdd BLAST105

Domaini

The second albumin domain forms a deep binding pocket that contains palmitoleic acid (in vitro). Palmitoleic acid is most likely not the physiological ligand. Instead, this pocket may accomodate the covalently bound lipid moiety of Wnt family members.By similarity

Sequence similaritiesi

Belongs to the ALB/AFP/VDB family.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IIRZ Eukaryota
ENOG410Z40H LUCA
HOGENOMiHOG000293137
HOVERGENiHBG004207
InParanoidiP36953
PhylomeDBiP36953

Family and domain databases

CDDicd00015 ALBUMIN, 3 hits
InterProiView protein in InterPro
IPR000264 ALB/AFP/VDB
IPR020858 Serum_albumin-like
IPR021177 Serum_albumin/AFP/Afamin
IPR020857 Serum_albumin_CS
IPR014760 Serum_albumin_N
PANTHERiPTHR11385 PTHR11385, 1 hit
PfamiView protein in Pfam
PF00273 Serum_albumin, 3 hits
PIRSFiPIRSF002520 Serum_albumin_subgroup, 1 hit
PRINTSiPR00802 SERUMALBUMIN
SMARTiView protein in SMART
SM00103 ALBUMIN, 3 hits
SUPFAMiSSF48552 SSF48552, 3 hits
PROSITEiView protein in PROSITE
PS00212 ALBUMIN_1, 1 hit
PS51438 ALBUMIN_2, 3 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36953-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRHLKLTGFI FFLLSLTESL ALPTKPQDVD HFNATQKFIN ENVAYLTIIA
60 70 80 90 100
SAQYVQEASF EEVEMLVKVM LDYKDRCLAD STLPECSKIA NDAIQDMLCD
110 120 130 140 150
MKGLPQKHNF SHCCRQAGFQ RRLCFFYNKK ANVGFLPPFP TLDPEEKCQA
160 170 180 190 200
YKNNSESFLN LYMYEVARRN PFAFAPVLLN VAARFEEAAT TCCEQQQKAT
210 220 230 240 250
YFQDKAAPIT QYLKALSSYQ RNVCGALLKF GPKTLNSINI AVFSKKFPKI
260 270 280 290 300
GFEDLTSLLE DVSSMYDGCC EGDVVQCIRS QSQVMHHICS KQDSISSKIK
310 320 330 340 350
ACCEKKLPER ADCIINANKD DRPEDLSLRT PKFTDSENVC QERDSEQDKF
360 370 380 390 400
FAEFLYDYSR RHTELSTPEL LRITKVYKDL LEDCCNRKNP LSCYRHAEDK
410 420 430 440 450
FNETTERSLA MVQQECKQFQ ELGKDALQRH FLVKFTKAAP QLPMEELVSL
460 470 480 490 500
SKEMVAALAT CCTLSDEFAC VDNLADLVLG ELCGMNKNRT INPTVDHCCR
510 520 530 540 550
ADFAFRRPCF EHLKADTTYA LPSVSALVSA LRADWCQPLK EDLQNKRHRF
560 570 580 590 600
LVNLVKWMPE ITDEERLCLF TKFTAAGEEC GNIQKPEACF SPESSKTGDV

SQDAEKQR
Length:608
Mass (Da):69,335
Last modified:June 1, 1994 - v1
Checksum:iF33151A6E68A07F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76456 Genomic DNA Translation: CAA53994.1
PIRiA53195
RefSeqiNP_758823.1, NM_172320.1
UniGeneiRn.214035

Genome annotation databases

GeneIDi282708
KEGGirno:282708
UCSCiRGD:628614 rat

Similar proteinsi

Entry informationi

Entry nameiAFAM_RAT
AccessioniPrimary (citable) accession number: P36953
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 23, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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