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P36952

- SPB5_HUMAN

UniProt

P36952 - SPB5_HUMAN

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Protein
Serpin B5
Gene
SERPINB5, PI5
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei340 – 3412Reactive bond homolog By similarity

GO - Molecular functioni

  1. protein binding Source: UniProtKB
  2. serine-type endopeptidase inhibitor activity Source: RefGenome

GO - Biological processi

  1. cellular component movement Source: ProtInc
  2. extracellular matrix organization Source: Ensembl
  3. morphogenesis of an epithelium Source: Ensembl
  4. negative regulation of endopeptidase activity Source: RefGenome
  5. prostate gland morphogenesis Source: Ensembl
  6. regulation of epithelial cell proliferation Source: Ensembl
  7. regulation of proteolysis Source: RefGenome
Complete GO annotation...

Protein family/group databases

MEROPSiI04.980.

Names & Taxonomyi

Protein namesi
Recommended name:
Serpin B5
Alternative name(s):
Maspin
Peptidase inhibitor 5
Short name:
PI-5
Gene namesi
Synonyms:PI5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 18

Organism-specific databases

HGNCiHGNC:8949. SERPINB5.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. extracellular space Source: UniProtKB-SubCell
  3. extracellular vesicular exosome Source: UniProt
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA35515.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 375375Serpin B5
PRO_0000032486Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi99 – 991N-linked (GlcNAc...) Reviewed prediction
Glycosylationi133 – 1331N-linked (GlcNAc...) Reviewed prediction
Glycosylationi188 – 1881N-linked (GlcNAc...) Reviewed prediction
Glycosylationi361 – 3611N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP36952.
PaxDbiP36952.
PRIDEiP36952.

PTM databases

PhosphoSiteiP36952.

Expressioni

Tissue specificityi

Normal mammary epithelial cells.

Gene expression databases

ArrayExpressiP36952.
BgeeiP36952.
CleanExiHS_SERPINB5.
GenevestigatoriP36952.

Organism-specific databases

HPAiCAB009570.
HPA019025.
HPA019132.
HPA020136.

Interactioni

Subunit structurei

Interacts with IRF6.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PRSS21Q9Y6M07EBI-2371394,EBI-7054564

Protein-protein interaction databases

BioGridi111286. 8 interactions.
IntActiP36952. 3 interactions.
MINTiMINT-7712082.
STRINGi9606.ENSP00000372221.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 2221
Beta strandi28 – 303
Helixi32 – 4514
Helixi48 – 5710
Helixi60 – 623
Helixi66 – 8015
Turni81 – 833
Beta strandi84 – 9512
Helixi96 – 983
Helixi102 – 1087
Turni109 – 1157
Beta strandi116 – 1194
Turni121 – 1233
Helixi125 – 13915
Turni140 – 1423
Turni147 – 1504
Beta strandi159 – 16810
Beta strandi171 – 1733
Helixi177 – 1793
Beta strandi181 – 19010
Beta strandi192 – 20918
Turni210 – 2134
Beta strandi214 – 2218
Helixi222 – 2243
Beta strandi225 – 23511
Helixi239 – 24911
Helixi252 – 2587
Helixi261 – 2633
Beta strandi265 – 27410
Beta strandi276 – 2827
Helixi284 – 2918
Turni295 – 2973
Turni299 – 3013
Turni305 – 3073
Beta strandi315 – 32612
Turni337 – 3415
Beta strandi344 – 3496
Beta strandi354 – 3607
Turni361 – 3644
Beta strandi365 – 3728

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WZ9X-ray2.10A/B1-375[»]
1XQGX-ray3.10A/B1-375[»]
1XQJX-ray3.10A1-375[»]
1XU8X-ray2.80A/B1-375[»]
ProteinModelPortaliP36952.
SMRiP36952. Positions 1-375.

Miscellaneous databases

EvolutionaryTraceiP36952.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG4826.
HOGENOMiHOG000238519.
HOVERGENiHBG005957.
InParanoidiP36952.
KOiK10139.
OMAiDTADQMK.
OrthoDBiEOG7327PB.
PhylomeDBiP36952.
TreeFamiTF352619.

Family and domain databases

InterProiIPR000240. Serpin_B9/Maspin.
IPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
PRINTSiPR00676. MASPIN.
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P36952-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MDALQLANSA FAVDLFKQLC EKEPLGNVLF SPICLSTSLS LAQVGAKGDT    50
ANEIGQVLHF ENVKDVPFGF QTVTSDVNKL SSFYSLKLIK RLYVDKSLNL 100
STEFISSTKR PYAKELETVD FKDKLEETKG QINNSIKDLT DGHFENILAD 150
NSVNDQTKIL VVNAAYFVGK WMKKFSESET KECPFRVNKT DTKPVQMMNM 200
EATFCMGNID SINCKIIELP FQNKHLSMFI LLPKDVEDES TGLEKIEKQL 250
NSESLSQWTN PSTMANAKVK LSIPKFKVEK MIDPKACLEN LGLKHIFSED 300
TSDFSGMSET KGVALSNVIH KVCLEITEDG GDSIEVPGAR ILQHKDELNA 350
DHPFIYIIRH NKTRNIIFFG KFCSP 375
Length:375
Mass (Da):42,100
Last modified:May 5, 2009 - v2
Checksum:i9F24E18505912804
GO
Isoform 2 (identifier: P36952-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     190-231: TDTKPVQMMN...QNKHLSMFIL → VCGAACSSKR...LRARPAKCLS
     232-375: Missing.

Show »
Length:231
Mass (Da):25,763
Checksum:i62DF767BBA9B5471
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti176 – 1761S → P.4 Publications
Corresponds to variant rs2289519 [ dbSNP | Ensembl ].
VAR_055223
Natural varianti187 – 1871V → L.1 Publication
Corresponds to variant rs2289520 [ dbSNP | Ensembl ].
VAR_055224
Natural varianti319 – 3191I → V.1 Publication
Corresponds to variant rs1455555 [ dbSNP | Ensembl ].
VAR_022115

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei190 – 23142TDTKP…SMFIL → VCGAACSSKRSPIIDVKNDR DRVGHKSIPMRNLRARPAKC LS in isoform 2.
VSP_037145Add
BLAST
Alternative sequencei232 – 375144Missing in isoform 2.
VSP_037146Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti66 – 661V → I in AAA18957. 1 Publication
Sequence conflicti245 – 2451K → Q in CAE45703. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U04313 mRNA. Translation: AAA18957.1.
AK312765 mRNA. Translation: BAG35631.1.
AC036176 Genomic DNA. No translation available.
BC020713 mRNA. Translation: AAH20713.1.
BX640597 mRNA. Translation: CAE45703.1.
CCDSiCCDS32839.1. [P36952-1]
PIRiA36898.
RefSeqiNP_002630.2. NM_002639.4. [P36952-1]
UniGeneiHs.55279.

Genome annotation databases

EnsembliENST00000382771; ENSP00000372221; ENSG00000206075. [P36952-1]
ENST00000489441; ENSP00000467158; ENSG00000206075. [P36952-2]
GeneIDi5268.
KEGGihsa:5268.
UCSCiuc002liy.2. human. [P36952-2]
uc002liz.4. human. [P36952-1]

Polymorphism databases

DMDMi229462757.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U04313 mRNA. Translation: AAA18957.1 .
AK312765 mRNA. Translation: BAG35631.1 .
AC036176 Genomic DNA. No translation available.
BC020713 mRNA. Translation: AAH20713.1 .
BX640597 mRNA. Translation: CAE45703.1 .
CCDSi CCDS32839.1. [P36952-1 ]
PIRi A36898.
RefSeqi NP_002630.2. NM_002639.4. [P36952-1 ]
UniGenei Hs.55279.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1WZ9 X-ray 2.10 A/B 1-375 [» ]
1XQG X-ray 3.10 A/B 1-375 [» ]
1XQJ X-ray 3.10 A 1-375 [» ]
1XU8 X-ray 2.80 A/B 1-375 [» ]
ProteinModelPortali P36952.
SMRi P36952. Positions 1-375.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 111286. 8 interactions.
IntActi P36952. 3 interactions.
MINTi MINT-7712082.
STRINGi 9606.ENSP00000372221.

Protein family/group databases

MEROPSi I04.980.

PTM databases

PhosphoSitei P36952.

Polymorphism databases

DMDMi 229462757.

Proteomic databases

MaxQBi P36952.
PaxDbi P36952.
PRIDEi P36952.

Protocols and materials databases

DNASUi 5268.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000382771 ; ENSP00000372221 ; ENSG00000206075 . [P36952-1 ]
ENST00000489441 ; ENSP00000467158 ; ENSG00000206075 . [P36952-2 ]
GeneIDi 5268.
KEGGi hsa:5268.
UCSCi uc002liy.2. human. [P36952-2 ]
uc002liz.4. human. [P36952-1 ]

Organism-specific databases

CTDi 5268.
GeneCardsi GC18P061117.
H-InvDB HIX0014501.
HGNCi HGNC:8949. SERPINB5.
HPAi CAB009570.
HPA019025.
HPA019132.
HPA020136.
MIMi 154790. gene.
neXtProti NX_P36952.
PharmGKBi PA35515.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG4826.
HOGENOMi HOG000238519.
HOVERGENi HBG005957.
InParanoidi P36952.
KOi K10139.
OMAi DTADQMK.
OrthoDBi EOG7327PB.
PhylomeDBi P36952.
TreeFami TF352619.

Miscellaneous databases

ChiTaRSi SERPINB5. human.
EvolutionaryTracei P36952.
GeneWikii Maspin.
GenomeRNAii 5268.
NextBioi 20352.
PROi P36952.
SOURCEi Search...

Gene expression databases

ArrayExpressi P36952.
Bgeei P36952.
CleanExi HS_SERPINB5.
Genevestigatori P36952.

Family and domain databases

InterProi IPR000240. Serpin_B9/Maspin.
IPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view ]
PANTHERi PTHR11461. PTHR11461. 1 hit.
Pfami PF00079. Serpin. 1 hit.
[Graphical view ]
PRINTSi PR00676. MASPIN.
SMARTi SM00093. SERPIN. 1 hit.
[Graphical view ]
SUPFAMi SSF56574. SSF56574. 1 hit.
PROSITEi PS00284. SERPIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Maspin, a serpin with tumor-suppressing activity in human mammary epithelial cells."
    Zou Z., Anisowicz A., Hendrix M.J.C., Thor A., Neveu M., Sheng S., Rafidi K., Seftor E., Sager R.
    Science 263:526-529(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS PRO-176 AND LEU-187.
    Tissue: Mammary gland.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT PRO-176.
    Tissue: Esophagus.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT PRO-176.
    Tissue: Prostate.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 245-375 (ISOFORM 1), VARIANT VAL-319.
    Tissue: Small intestine.
  6. "The tumor suppressor maspin does not undergo the stressed to relaxed transition or inhibit trypsin-like serine proteases. Evidence that maspin is not a protease inhibitory serpin."
    Pemberton P.A., Wong D.T., Gibson H.L., Kiefer M.C., Fitzpatrick P.A., Sager R., Barr P.J.
    J. Biol. Chem. 270:15832-15837(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 341-360, CHARACTERIZATION.
  7. "Mammary serine protease inhibitor (Maspin) binds directly to interferon regulatory factor 6: identification of a novel serpin partnership."
    Bailey C.M., Khalkhali-Ellis Z., Kondo S., Margaryan N.V., Seftor R.E.B., Wheaton W.W., Amir S., Pins M.R., Schutte B.C., Hendrix M.J.C.
    J. Biol. Chem. 280:34210-34217(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH IRF6.
  8. "The high resolution crystal structure of the human tumor suppressor maspin reveals a novel conformational switch in the G-helix."
    Law R.H., Irving J.A., Buckle A.M., Ruzyla K., Buzza M., Bashtannyk-Puhalovich T.A., Beddoe T.C., Nguyen K., Worrall D.M., Bottomley S.P., Bird P.I., Rossjohn J., Whisstock J.C.
    J. Biol. Chem. 280:22356-22364(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
  9. Cited for: VARIANT [LARGE SCALE ANALYSIS] PRO-176, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSPB5_HUMAN
AccessioniPrimary (citable) accession number: P36952
Secondary accession number(s): B2R6Y4, Q6N0B4, Q8WW89
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: May 5, 2009
Last modified: September 3, 2014
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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