Reviewed, UniProtKB/Swiss-Prot P36952 (SPB5_HUMAN)

Names and origin

Protein names

Recommended name:
    Serpin B5
Alternative name(s):
    Protease inhibitor 5
    Maspin

Gene names

Name:	SERPINB5
Synonyms:	PI5

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
Subcellular location	Secreted › extracellular space.
Tissue specificity	Normal mammary epithelial cells.
Sequence similarities	Belongs to the serpin family. Ov-serpin subfamily.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
PTM	Glycoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	cell motion Ref.1 Traceable author statement. Source: ProtInc
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – ?

Potential

Chain

? – 375

Serpin B5

PRO_0000032486

Sites

Site

340 – 341

2

Reactive bond homolog By similarity

Amino acid modifications

Glycosylation

99

1

N-linked (GlcNAc...) Potential

Glycosylation

133

1

N-linked (GlcNAc...) Potential

Glycosylation

188

1

N-linked (GlcNAc...) Potential

Glycosylation

361

1

N-linked (GlcNAc...) Potential

Natural variations

Natural variant

319

1

I → V: dbSNP rs1455555.

VAR_022115

Secondary structure

1

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375

Helix Strand Turn

Details...

Helix

2 – 22

21

Helix

32 – 45

14

Helix

48 – 57

10

Helix

66 – 80

15

Turn

81 – 83

3

Beta strand

84 – 95

12

Helix

96 – 98

3

Helix

102 – 108

7

Turn

109 – 115

7

Beta strand

116 – 119

4

Turn

121 – 123

3

Helix

125 – 139

15

Turn

140 – 142

3

Turn

147 – 150

4

Beta strand

159 – 168

10

Beta strand

171 – 173

3

Helix

177 – 179

3

Beta strand

181 – 190

10

Beta strand

192 – 209

18

Turn

210 – 213

4

Beta strand

214 – 221

8

Helix

222 – 224

3

Beta strand

225 – 235

11

Helix

239 – 249

11

Helix

252 – 258

7

Helix

261 – 263

3

Beta strand

265 – 274

10

Beta strand

276 – 282

7

Helix

284 – 291

8

Turn

299 – 301

3

Turn

305 – 307

3

Beta strand

315 – 326

12

Beta strand

344 – 349

6

Beta strand

354 – 360

7

Turn

361 – 364

4

Beta strand

365 – 372

8

Sequences

	Sequence		Length	Mass (Da)
	P36952-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: F2D49FFCD6AC3DC2	FASTA	375	42,138
	10 20 30 40 50 60 MDALQLANSA FAVDLFKQLC EKEPLGNVLF SPICLSTSLS LAQVGAKGDT ANEIGQVLHF 70 80 90 100 110 120 ENVKDIPFGF QTVTSDVNKL SSFYSLKLIK RLYVDKSLNL STEFISSTKR PYAKELETVD 130 140 150 160 170 180 FKDKLEETKG QINNSIKDLT DGHFENILAD NSVNDQTKIL VVNAAYFVGK WMKKFPESET 190 200 210 220 230 240 KECPFRLNKT DTKPVQMMNM EATFCMGNID SINCKIIELP FQNKHLSMFI LLPKDVEDES 250 260 270 280 290 300 TGLEKIEKQL NSESLSQWTN PSTMANAKVK LSIPKFKVEK MIDPKACLEN LGLKHIFSED 310 320 330 340 350 360 TSDFSGMSET KGVALSNVIH KVCLEITEDG GDSIEVPGAR ILQHKDELNA DHPFIYIIRH 370 NKTRNIIFFG KFCSP

References

[1]	"Maspin, a serpin with tumor-suppressing activity in human mammary epithelial cells." Zou Z., Anisowicz A., Hendrix M.J.C., Thor A., Neveu M., Sheng S., Rafidi K., Seftor E., Sager R. Science 263:526-529(1994) [PubMed: 8290962] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Mammary gland.
[2]	"The tumor suppressor maspin does not undergo the stressed to relaxed transition or inhibit trypsin-like serine proteases. Evidence that maspin is not a protease inhibitory serpin." Pemberton P.A., Wong D.T., Gibson H.L., Kiefer M.C., Fitzpatrick P.A., Sager R., Barr P.J. J. Biol. Chem. 270:15832-15837(1995) [PubMed: 7797587] [Abstract] Cited for: PROTEIN SEQUENCE OF 341-360, CHARACTERIZATION.
[3]	"The high resolution crystal structure of the human tumor suppressor maspin reveals a novel conformational switch in the G-helix." Law R.H., Irving J.A., Buckle A.M., Ruzyla K., Buzza M., Bashtannyk-Puhalovich T.A., Beddoe T.C., Nguyen K., Worrall D.M., Bottomley S.P., Bird P.I., Rossjohn J., Whisstock J.C. J. Biol. Chem. 280:22356-22364(2005) [PubMed: 15760906] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases

EMBLGenBankDDBJ

U04313 mRNA. Translation: AAA18957.1.

PIR

A36898.

UniGene

Hs.55279

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WZ9	X-ray	2.10	A/B	1-375	[»]
1XQG	X-ray	3.10	A/B	1-375	[»]
1XQJ	X-ray	3.10	A	1-375	[»]
1XU8	X-ray	2.80	A/B	1-375	[»]

ModBase

Search...

Protein family/group databases

MEROPS

I04.980.

2-D gel databases

Cornea-2DPAGE

P36952.

Genome annotation databases

Ensembl

ENSG00000206075. Homo sapiens. [Contig view]

Organism-specific databases

H-InvDB

HIX0014501.

HGNC

HGNC:8949. SERPINB5.

HPA

CAB009570.

MIM

154790. gene.

PharmGKB

PA35515.

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOVERGEN

P36952.

Gene expression databases

ArrayExpress

P36952.

CleanEx

HS_SERPINB5.

GermOnline

ENSG00000206075. Homo sapiens.

Family and domain databases

InterPro

IPR000215. Protease_inhib_I4_serpin.
IPR000240. SerpinB9_Maspin.
[Graphical view]

PANTHER

PTHR11461. Prot_inh_serpin. 1 hit.

Pfam

PF00079. Serpin. 1 hit.
[Graphical view]

PRINTS

PR00676. MASPIN.

SMART

SM00093. SERPIN. 1 hit.
[Graphical view]

PROSITE

PS00284. SERPIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

SOURCE

Search...

Entry information

Entry name

SPB5_HUMAN

Accession

Primary (citable) accession number: P36952

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	November 25, 2008
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)