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Protein

Serpin B5

Gene

SERPINB5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei340 – 341Reactive bond homologBy similarity2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000166628-MONOMER.

Protein family/group databases

MEROPSiI04.980.

Names & Taxonomyi

Protein namesi
Recommended name:
Serpin B5
Alternative name(s):
Maspin
Peptidase inhibitor 5
Short name:
PI-5
Gene namesi
Name:SERPINB5
Synonyms:PI5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

HGNCiHGNC:8949. SERPINB5.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • extracellular space Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi5268.
OpenTargetsiENSG00000206075.
PharmGKBiPA35515.

Polymorphism and mutation databases

BioMutaiSERPINB5.
DMDMi229462757.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000324861 – 375Serpin B5Add BLAST375

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi99N-linked (GlcNAc...)Sequence analysis1
Glycosylationi133N-linked (GlcNAc...)Sequence analysis1
Glycosylationi188N-linked (GlcNAc...)Sequence analysis1
Glycosylationi361N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiP36952.
MaxQBiP36952.
PaxDbiP36952.
PeptideAtlasiP36952.
PRIDEiP36952.
TopDownProteomicsiP36952-1. [P36952-1]

PTM databases

iPTMnetiP36952.
PhosphoSitePlusiP36952.

Expressioni

Tissue specificityi

Normal mammary epithelial cells.

Gene expression databases

BgeeiENSG00000206075.
CleanExiHS_SERPINB5.
ExpressionAtlasiP36952. baseline and differential.
GenevisibleiP36952. HS.

Organism-specific databases

HPAiCAB009570.
HPA019025.
HPA019132.
HPA020136.

Interactioni

Subunit structurei

Interacts with IRF6.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PRSS21Q9Y6M07EBI-2371394,EBI-7054564

Protein-protein interaction databases

BioGridi111286. 70 interactors.
IntActiP36952. 12 interactors.
MINTiMINT-7712082.
STRINGi9606.ENSP00000372221.

Structurei

Secondary structure

1375
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 22Combined sources21
Beta strandi28 – 30Combined sources3
Helixi32 – 45Combined sources14
Helixi48 – 57Combined sources10
Helixi60 – 62Combined sources3
Helixi66 – 80Combined sources15
Turni81 – 83Combined sources3
Beta strandi84 – 95Combined sources12
Helixi96 – 98Combined sources3
Helixi102 – 108Combined sources7
Turni109 – 115Combined sources7
Beta strandi116 – 119Combined sources4
Turni121 – 123Combined sources3
Helixi125 – 139Combined sources15
Turni140 – 142Combined sources3
Turni147 – 150Combined sources4
Beta strandi159 – 168Combined sources10
Beta strandi171 – 173Combined sources3
Helixi177 – 179Combined sources3
Beta strandi181 – 190Combined sources10
Beta strandi192 – 209Combined sources18
Turni210 – 213Combined sources4
Beta strandi214 – 221Combined sources8
Helixi222 – 224Combined sources3
Beta strandi225 – 235Combined sources11
Helixi239 – 249Combined sources11
Helixi252 – 258Combined sources7
Helixi261 – 263Combined sources3
Beta strandi265 – 274Combined sources10
Beta strandi276 – 282Combined sources7
Helixi284 – 291Combined sources8
Turni295 – 297Combined sources3
Turni299 – 301Combined sources3
Turni305 – 307Combined sources3
Beta strandi315 – 326Combined sources12
Turni337 – 341Combined sources5
Beta strandi344 – 349Combined sources6
Beta strandi354 – 360Combined sources7
Turni361 – 364Combined sources4
Beta strandi365 – 372Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WZ9X-ray2.10A/B1-375[»]
1XQGX-ray3.10A/B1-375[»]
1XQJX-ray3.10A1-375[»]
1XU8X-ray2.80A/B1-375[»]
ProteinModelPortaliP36952.
SMRiP36952.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP36952.

Family & Domainsi

Sequence similaritiesi

Belongs to the serpin family. Ov-serpin subfamily.Curated

Phylogenomic databases

eggNOGiKOG2392. Eukaryota.
COG4826. LUCA.
GeneTreeiENSGT00760000118789.
HOGENOMiHOG000238519.
HOVERGENiHBG005957.
InParanoidiP36952.
KOiK10139.
OMAiXEFISST.
OrthoDBiEOG091G0ION.
PhylomeDBiP36952.
TreeFamiTF352619.

Family and domain databases

CDDicd02057. maspin_like. 1 hit.
InterProiIPR000240. Serpin_B9/Maspin.
IPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
IPR033836. SERPINB5.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
PRINTSiPR00676. MASPIN.
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P36952-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDALQLANSA FAVDLFKQLC EKEPLGNVLF SPICLSTSLS LAQVGAKGDT
60 70 80 90 100
ANEIGQVLHF ENVKDVPFGF QTVTSDVNKL SSFYSLKLIK RLYVDKSLNL
110 120 130 140 150
STEFISSTKR PYAKELETVD FKDKLEETKG QINNSIKDLT DGHFENILAD
160 170 180 190 200
NSVNDQTKIL VVNAAYFVGK WMKKFSESET KECPFRVNKT DTKPVQMMNM
210 220 230 240 250
EATFCMGNID SINCKIIELP FQNKHLSMFI LLPKDVEDES TGLEKIEKQL
260 270 280 290 300
NSESLSQWTN PSTMANAKVK LSIPKFKVEK MIDPKACLEN LGLKHIFSED
310 320 330 340 350
TSDFSGMSET KGVALSNVIH KVCLEITEDG GDSIEVPGAR ILQHKDELNA
360 370
DHPFIYIIRH NKTRNIIFFG KFCSP
Length:375
Mass (Da):42,100
Last modified:May 5, 2009 - v2
Checksum:i9F24E18505912804
GO
Isoform 2 (identifier: P36952-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     190-231: TDTKPVQMMN...QNKHLSMFIL → VCGAACSSKR...LRARPAKCLS
     232-375: Missing.

Show »
Length:231
Mass (Da):25,763
Checksum:i62DF767BBA9B5471
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti66V → I in AAA18957 (PubMed:8290962).Curated1
Sequence conflicti245K → Q in CAE45703 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_055223176S → P.Combined sources3 PublicationsCorresponds to variant rs2289519dbSNPEnsembl.1
Natural variantiVAR_055224187V → L.1 PublicationCorresponds to variant rs2289520dbSNPEnsembl.1
Natural variantiVAR_022115319I → V.1 PublicationCorresponds to variant rs1455555dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_037145190 – 231TDTKP…SMFIL → VCGAACSSKRSPIIDVKNDR DRVGHKSIPMRNLRARPAKC LS in isoform 2. 1 PublicationAdd BLAST42
Alternative sequenceiVSP_037146232 – 375Missing in isoform 2. 1 PublicationAdd BLAST144

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04313 mRNA. Translation: AAA18957.1.
AK312765 mRNA. Translation: BAG35631.1.
AC036176 Genomic DNA. No translation available.
BC020713 mRNA. Translation: AAH20713.1.
BX640597 mRNA. Translation: CAE45703.1.
CCDSiCCDS32839.1. [P36952-1]
PIRiA36898.
RefSeqiNP_002630.2. NM_002639.4. [P36952-1]
UniGeneiHs.55279.

Genome annotation databases

EnsembliENST00000382771; ENSP00000372221; ENSG00000206075. [P36952-1]
ENST00000489441; ENSP00000467158; ENSG00000206075. [P36952-2]
GeneIDi5268.
KEGGihsa:5268.
UCSCiuc002liy.3. human. [P36952-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04313 mRNA. Translation: AAA18957.1.
AK312765 mRNA. Translation: BAG35631.1.
AC036176 Genomic DNA. No translation available.
BC020713 mRNA. Translation: AAH20713.1.
BX640597 mRNA. Translation: CAE45703.1.
CCDSiCCDS32839.1. [P36952-1]
PIRiA36898.
RefSeqiNP_002630.2. NM_002639.4. [P36952-1]
UniGeneiHs.55279.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WZ9X-ray2.10A/B1-375[»]
1XQGX-ray3.10A/B1-375[»]
1XQJX-ray3.10A1-375[»]
1XU8X-ray2.80A/B1-375[»]
ProteinModelPortaliP36952.
SMRiP36952.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111286. 70 interactors.
IntActiP36952. 12 interactors.
MINTiMINT-7712082.
STRINGi9606.ENSP00000372221.

Protein family/group databases

MEROPSiI04.980.

PTM databases

iPTMnetiP36952.
PhosphoSitePlusiP36952.

Polymorphism and mutation databases

BioMutaiSERPINB5.
DMDMi229462757.

Proteomic databases

EPDiP36952.
MaxQBiP36952.
PaxDbiP36952.
PeptideAtlasiP36952.
PRIDEiP36952.
TopDownProteomicsiP36952-1. [P36952-1]

Protocols and materials databases

DNASUi5268.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000382771; ENSP00000372221; ENSG00000206075. [P36952-1]
ENST00000489441; ENSP00000467158; ENSG00000206075. [P36952-2]
GeneIDi5268.
KEGGihsa:5268.
UCSCiuc002liy.3. human. [P36952-1]

Organism-specific databases

CTDi5268.
DisGeNETi5268.
GeneCardsiSERPINB5.
H-InvDBHIX0014501.
HGNCiHGNC:8949. SERPINB5.
HPAiCAB009570.
HPA019025.
HPA019132.
HPA020136.
MIMi154790. gene.
neXtProtiNX_P36952.
OpenTargetsiENSG00000206075.
PharmGKBiPA35515.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2392. Eukaryota.
COG4826. LUCA.
GeneTreeiENSGT00760000118789.
HOGENOMiHOG000238519.
HOVERGENiHBG005957.
InParanoidiP36952.
KOiK10139.
OMAiXEFISST.
OrthoDBiEOG091G0ION.
PhylomeDBiP36952.
TreeFamiTF352619.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000166628-MONOMER.

Miscellaneous databases

ChiTaRSiSERPINB5. human.
EvolutionaryTraceiP36952.
GeneWikiiMaspin.
GenomeRNAii5268.
PROiP36952.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000206075.
CleanExiHS_SERPINB5.
ExpressionAtlasiP36952. baseline and differential.
GenevisibleiP36952. HS.

Family and domain databases

CDDicd02057. maspin_like. 1 hit.
InterProiIPR000240. Serpin_B9/Maspin.
IPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
IPR033836. SERPINB5.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
PRINTSiPR00676. MASPIN.
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPB5_HUMAN
AccessioniPrimary (citable) accession number: P36952
Secondary accession number(s): B2R6Y4, Q6N0B4, Q8WW89
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: May 5, 2009
Last modified: November 30, 2016
This is version 156 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.