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Reviewed, UniProtKB/Swiss-Prot P36952 (SPB5_HUMAN)

Last modified November 25, 2008. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Serpin B5
Alternative name(s):
    Protease inhibitor 5
    Maspin
Gene names
Name: SERPINB5
Synonyms: PI5
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length375 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

Subcellular location

Secretedextracellular space.

Tissue specificity

Normal mammary epithelial cells.

Sequence similarities

Belongs to the serpin family. Ov-serpin subfamily.

Ontologies

Keywords

   Cellular componentSecreted
   Coding sequence diversityPolymorphism
   DomainSignal
   PTMGlycoprotein
   Technical term3D-structure
Direct protein sequencing

Gene Ontology (GO)

   Biological processcell motion Ref.1

Traceable author statement. Source: ProtInc

   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionserine-type endopeptidase inhibitor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – ? Potential
Chain? – 375Serpin B5PRO_0000032486

Sites

Site340 – 3412Reactive bond homolog By similarity

Amino acid modifications

Glycosylation991N-linked (GlcNAc...) Potential
Glycosylation1331N-linked (GlcNAc...) Potential
Glycosylation1881N-linked (GlcNAc...) Potential
Glycosylation3611N-linked (GlcNAc...) Potential

Natural variations

Natural variant3191I → V: dbSNP rs1455555.
VAR_022115

Secondary structure

............................................................. 375
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P36952-1 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: F2D49FFCD6AC3DC2

FASTA37542,138
        10         20         30         40         50         60 
MDALQLANSA FAVDLFKQLC EKEPLGNVLF SPICLSTSLS LAQVGAKGDT ANEIGQVLHF 

        70         80         90        100        110        120 
ENVKDIPFGF QTVTSDVNKL SSFYSLKLIK RLYVDKSLNL STEFISSTKR PYAKELETVD 

       130        140        150        160        170        180 
FKDKLEETKG QINNSIKDLT DGHFENILAD NSVNDQTKIL VVNAAYFVGK WMKKFPESET 

       190        200        210        220        230        240 
KECPFRLNKT DTKPVQMMNM EATFCMGNID SINCKIIELP FQNKHLSMFI LLPKDVEDES 

       250        260        270        280        290        300 
TGLEKIEKQL NSESLSQWTN PSTMANAKVK LSIPKFKVEK MIDPKACLEN LGLKHIFSED 

       310        320        330        340        350        360 
TSDFSGMSET KGVALSNVIH KVCLEITEDG GDSIEVPGAR ILQHKDELNA DHPFIYIIRH 

       370 
NKTRNIIFFG KFCSP 

« Hide

References

[1]"Maspin, a serpin with tumor-suppressing activity in human mammary epithelial cells."
Zou Z., Anisowicz A., Hendrix M.J.C., Thor A., Neveu M., Sheng S., Rafidi K., Seftor E., Sager R.
Science 263:526-529(1994) [PubMed: 8290962] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Mammary gland.
[2]"The tumor suppressor maspin does not undergo the stressed to relaxed transition or inhibit trypsin-like serine proteases. Evidence that maspin is not a protease inhibitory serpin."
Pemberton P.A., Wong D.T., Gibson H.L., Kiefer M.C., Fitzpatrick P.A., Sager R., Barr P.J.
J. Biol. Chem. 270:15832-15837(1995) [PubMed: 7797587] [Abstract]
Cited for: PROTEIN SEQUENCE OF 341-360, CHARACTERIZATION.
[3]"The high resolution crystal structure of the human tumor suppressor maspin reveals a novel conformational switch in the G-helix."
Law R.H., Irving J.A., Buckle A.M., Ruzyla K., Buzza M., Bashtannyk-Puhalovich T.A., Beddoe T.C., Nguyen K., Worrall D.M., Bottomley S.P., Bird P.I., Rossjohn J., Whisstock J.C.
J. Biol. Chem. 280:22356-22364(2005) [PubMed: 15760906] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

U04313 mRNA. Translation: AAA18957.1.
PIRA36898.
UniGeneHs.55279

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1WZ9X-ray2.10A/B1-375[»]
1XQGX-ray3.10A/B1-375[»]
1XQJX-ray3.10A1-375[»]
1XU8X-ray2.80A/B1-375[»]
ModBaseSearch...

Protein family/group databases

MEROPSI04.980.

2-D gel databases

Cornea-2DPAGEP36952.

Genome annotation databases

EnsemblENSG00000206075. Homo sapiens. [Contig view]

Organism-specific databases

H-InvDBHIX0014501.
HGNCHGNC:8949. SERPINB5.
HPACAB009570.
MIM154790. gene.
PharmGKBPA35515.
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOVERGENP36952.

Gene expression databases

ArrayExpressP36952.
CleanExHS_SERPINB5.
GermOnlineENSG00000206075. Homo sapiens.

Family and domain databases

InterProIPR000215. Protease_inhib_I4_serpin.
IPR000240. SerpinB9_Maspin.
[Graphical view]
PANTHERPTHR11461. Prot_inh_serpin. 1 hit.
PfamPF00079. Serpin. 1 hit.
[Graphical view]
PRINTSPR00676. MASPIN.
SMARTSM00093. SERPIN. 1 hit.
[Graphical view]
PROSITEPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

SOURCESearch...

Entry information

Entry nameSPB5_HUMAN
AccessionPrimary (citable) accession number: P36952
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 25, 2008
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 18

Human chromosome 18: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents