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P36952 (SPB5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serpin B5
Alternative name(s):
Maspin
Peptidase inhibitor 5
Short name=PI-5
Gene names
Name:SERPINB5
Synonyms:PI5
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length375 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

Subunit structure

Interacts with IRF6. Ref.7

Subcellular location

Secretedextracellular space.

Tissue specificity

Normal mammary epithelial cells.

Sequence similarities

Belongs to the serpin family. Ov-serpin subfamily.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PRSS21Q9Y6M07EBI-2371394,EBI-7054564

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P36952-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P36952-2)

The sequence of this isoform differs from the canonical sequence as follows:
     190-231: TDTKPVQMMN...QNKHLSMFIL → VCGAACSSKR...LRARPAKCLS
     232-375: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 375375Serpin B5
PRO_0000032486

Sites

Site340 – 3412Reactive bond homolog By similarity

Amino acid modifications

Glycosylation991N-linked (GlcNAc...) Potential
Glycosylation1331N-linked (GlcNAc...) Potential
Glycosylation1881N-linked (GlcNAc...) Potential
Glycosylation3611N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence190 – 23142TDTKP…SMFIL → VCGAACSSKRSPIIDVKNDR DRVGHKSIPMRNLRARPAKC LS in isoform 2.
VSP_037145
Alternative sequence232 – 375144Missing in isoform 2.
VSP_037146
Natural variant1761S → P. Ref.1 Ref.2 Ref.4 Ref.9
Corresponds to variant rs2289519 [ dbSNP | Ensembl ].
VAR_055223
Natural variant1871V → L. Ref.1
Corresponds to variant rs2289520 [ dbSNP | Ensembl ].
VAR_055224
Natural variant3191I → V. Ref.5
Corresponds to variant rs1455555 [ dbSNP | Ensembl ].
VAR_022115

Experimental info

Sequence conflict661V → I in AAA18957. Ref.1
Sequence conflict2451K → Q in CAE45703. Ref.5

Secondary structure

..................................................................... 375
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 5, 2009. Version 2.
Checksum: 9F24E18505912804

FASTA37542,100
        10         20         30         40         50         60 
MDALQLANSA FAVDLFKQLC EKEPLGNVLF SPICLSTSLS LAQVGAKGDT ANEIGQVLHF 

        70         80         90        100        110        120 
ENVKDVPFGF QTVTSDVNKL SSFYSLKLIK RLYVDKSLNL STEFISSTKR PYAKELETVD 

       130        140        150        160        170        180 
FKDKLEETKG QINNSIKDLT DGHFENILAD NSVNDQTKIL VVNAAYFVGK WMKKFSESET 

       190        200        210        220        230        240 
KECPFRVNKT DTKPVQMMNM EATFCMGNID SINCKIIELP FQNKHLSMFI LLPKDVEDES 

       250        260        270        280        290        300 
TGLEKIEKQL NSESLSQWTN PSTMANAKVK LSIPKFKVEK MIDPKACLEN LGLKHIFSED 

       310        320        330        340        350        360 
TSDFSGMSET KGVALSNVIH KVCLEITEDG GDSIEVPGAR ILQHKDELNA DHPFIYIIRH 

       370 
NKTRNIIFFG KFCSP 

« Hide

Isoform 2 [UniParc].

Checksum: 62DF767BBA9B5471
Show »

FASTA23125,763

References

« Hide 'large scale' references
[1]"Maspin, a serpin with tumor-suppressing activity in human mammary epithelial cells."
Zou Z., Anisowicz A., Hendrix M.J.C., Thor A., Neveu M., Sheng S., Rafidi K., Seftor E., Sager R.
Science 263:526-529(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS PRO-176 AND LEU-187.
Tissue: Mammary gland.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT PRO-176.
Tissue: Esophagus.
[3]"DNA sequence and analysis of human chromosome 18."
Nusbaum C., Zody M.C., Borowsky M.L., Kamal M., Kodira C.D., Taylor T.D., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Abouelleil A., Allen N.R., Anderson S., Bloom T., Bugalter B., Butler J. expand/collapse author list , Cook A., DeCaprio D., Engels R., Garber M., Gnirke A., Hafez N., Hall J.L., Norman C.H., Itoh T., Jaffe D.B., Kuroki Y., Lehoczky J., Lui A., Macdonald P., Mauceli E., Mikkelsen T.S., Naylor J.W., Nicol R., Nguyen C., Noguchi H., O'Leary S.B., Piqani B., Smith C.L., Talamas J.A., Topham K., Totoki Y., Toyoda A., Wain H.M., Young S.K., Zeng Q., Zimmer A.R., Fujiyama A., Hattori M., Birren B.W., Sakaki Y., Lander E.S.
Nature 437:551-555(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT PRO-176.
Tissue: Prostate.
[5]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 245-375 (ISOFORM 1), VARIANT VAL-319.
Tissue: Small intestine.
[6]"The tumor suppressor maspin does not undergo the stressed to relaxed transition or inhibit trypsin-like serine proteases. Evidence that maspin is not a protease inhibitory serpin."
Pemberton P.A., Wong D.T., Gibson H.L., Kiefer M.C., Fitzpatrick P.A., Sager R., Barr P.J.
J. Biol. Chem. 270:15832-15837(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 341-360, CHARACTERIZATION.
[7]"Mammary serine protease inhibitor (Maspin) binds directly to interferon regulatory factor 6: identification of a novel serpin partnership."
Bailey C.M., Khalkhali-Ellis Z., Kondo S., Margaryan N.V., Seftor R.E.B., Wheaton W.W., Amir S., Pins M.R., Schutte B.C., Hendrix M.J.C.
J. Biol. Chem. 280:34210-34217(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH IRF6.
[8]"The high resolution crystal structure of the human tumor suppressor maspin reveals a novel conformational switch in the G-helix."
Law R.H., Irving J.A., Buckle A.M., Ruzyla K., Buzza M., Bashtannyk-Puhalovich T.A., Beddoe T.C., Nguyen K., Worrall D.M., Bottomley S.P., Bird P.I., Rossjohn J., Whisstock J.C.
J. Biol. Chem. 280:22356-22364(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
[9]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] PRO-176, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U04313 mRNA. Translation: AAA18957.1.
AK312765 mRNA. Translation: BAG35631.1.
AC036176 Genomic DNA. No translation available.
BC020713 mRNA. Translation: AAH20713.1.
BX640597 mRNA. Translation: CAE45703.1.
PIRA36898.
RefSeqNP_002630.2. NM_002639.4.
UniGeneHs.55279.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1WZ9X-ray2.10A/B1-375[»]
1XQGX-ray3.10A/B1-375[»]
1XQJX-ray3.10A1-375[»]
1XU8X-ray2.80A/B1-375[»]
ProteinModelPortalP36952.
SMRP36952. Positions 1-375.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid111286. 7 interactions.
IntActP36952. 3 interactions.
MINTMINT-7712082.
STRING9606.ENSP00000372221.

Protein family/group databases

MEROPSI04.980.

PTM databases

PhosphoSiteP36952.

Polymorphism databases

DMDM229462757.

Proteomic databases

PaxDbP36952.
PRIDEP36952.

Protocols and materials databases

DNASU5268.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000382771; ENSP00000372221; ENSG00000206075. [P36952-1]
ENST00000489441; ENSP00000467158; ENSG00000206075. [P36952-2]
GeneID5268.
KEGGhsa:5268.
UCSCuc002liy.2. human. [P36952-2]
uc002liz.4. human. [P36952-1]

Organism-specific databases

CTD5268.
GeneCardsGC18P061117.
H-InvDBHIX0014501.
HGNCHGNC:8949. SERPINB5.
HPACAB009570.
HPA019025.
HPA019132.
HPA020136.
MIM154790. gene.
neXtProtNX_P36952.
PharmGKBPA35515.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG4826.
HOGENOMHOG000238519.
HOVERGENHBG005957.
InParanoidP36952.
KOK10139.
OMAXEFISST.
OrthoDBEOG7327PB.
PhylomeDBP36952.
TreeFamTF352619.

Gene expression databases

ArrayExpressP36952.
BgeeP36952.
CleanExHS_SERPINB5.
GenevestigatorP36952.

Family and domain databases

InterProIPR000240. Serpin_B9/Maspin.
IPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERPTHR11461. PTHR11461. 1 hit.
PfamPF00079. Serpin. 1 hit.
[Graphical view]
PRINTSPR00676. MASPIN.
SMARTSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMSSF56574. SSF56574. 1 hit.
PROSITEPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSERPINB5. human.
EvolutionaryTraceP36952.
GeneWikiMaspin.
GenomeRNAi5268.
NextBio20352.
PROP36952.
SOURCESearch...

Entry information

Entry nameSPB5_HUMAN
AccessionPrimary (citable) accession number: P36952
Secondary accession number(s): B2R6Y4, Q6N0B4, Q8WW89
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: May 5, 2009
Last modified: April 16, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 18

Human chromosome 18: entries, gene names and cross-references to MIM