Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphoglucomutase

Gene

pgm

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme participates in both the breakdown and synthesis of glucose.1 Publication

Catalytic activityi

Alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate.1 Publication

Cofactori

Mg2+1 PublicationNote: Binds 1 Mg2+ ion per subunit.1 Publication

Kineticsi

  1. KM=50 µM for D-glucose-1-phosphate1 Publication

    pH dependencei

    Optimum pH is 9.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei146 – 1461Phosphoserine intermediateBy similarity
    Metal bindingi146 – 1461Magnesium; via phosphate groupBy similarity
    Binding sitei156 – 1561SubstrateBy similarity
    Metal bindingi304 – 3041MagnesiumBy similarity
    Metal bindingi306 – 3061MagnesiumBy similarity
    Metal bindingi308 – 3081MagnesiumBy similarity
    Binding sitei409 – 4091SubstrateBy similarity

    GO - Molecular functioni

    • magnesium ion binding Source: InterPro
    • phosphoglucomutase activity Source: EcoCyc

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Carbohydrate metabolism, Glucose metabolism

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:PHOSPHOGLUCMUT-MONOMER.
    ECOL316407:JW0675-MONOMER.
    MetaCyc:PHOSPHOGLUCMUT-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoglucomutase (EC:5.4.2.2)
    Short name:
    PGM
    Alternative name(s):
    Glucose phosphomutase
    Gene namesi
    Name:pgm
    Ordered Locus Names:b0688, JW0675
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG12144. pgm.

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: EcoCyc
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 546546PhosphoglucomutasePRO_0000147809Add
    BLAST

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    EPDiP36938.
    PaxDbiP36938.
    PRIDEiP36938.

    Interactioni

    Protein-protein interaction databases

    BioGridi4261910. 16 interactions.
    DIPiDIP-10472N.
    IntActiP36938. 7 interactions.
    MINTiMINT-1240111.
    STRINGi511145.b0688.

    Structurei

    3D structure databases

    ProteinModelPortaliP36938.
    SMRiP36938. Positions 3-545.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni146 – 1472Substrate bindingBy similarity
    Regioni308 – 3092Substrate bindingBy similarity
    Regioni390 – 3923Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the phosphohexose mutase family.Curated

    Phylogenomic databases

    eggNOGiENOG4107QIX. Bacteria.
    COG0033. LUCA.
    HOGENOMiHOG000268677.
    InParanoidiP36938.
    KOiK01835.
    OMAiYYARHDY.
    PhylomeDBiP36938.

    Family and domain databases

    Gene3Di3.30.310.50. 1 hit.
    3.40.120.10. 3 hits.
    InterProiIPR005844. A-D-PHexomutase_a/b/a-I.
    IPR016055. A-D-PHexomutase_a/b/a-I/II/III.
    IPR005845. A-D-PHexomutase_a/b/a-II.
    IPR005846. A-D-PHexomutase_a/b/a-III.
    IPR005843. A-D-PHexomutase_C.
    IPR016066. A-D-PHexomutase_CS.
    IPR005852. PGlcMutase_a-D-Glc-sp.
    [Graphical view]
    PfamiPF02878. PGM_PMM_I. 1 hit.
    PF02879. PGM_PMM_II. 1 hit.
    PF02880. PGM_PMM_III. 1 hit.
    PF00408. PGM_PMM_IV. 1 hit.
    [Graphical view]
    SUPFAMiSSF53738. SSF53738. 3 hits.
    SSF55957. SSF55957. 1 hit.
    TIGRFAMsiTIGR01132. pgm. 1 hit.
    PROSITEiPS00710. PGM_PMM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P36938-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAIHNRAGQP AQQSDLINVA QLTAQYYVLK PEAGNAEHAV KFGTSGHRGS
    60 70 80 90 100
    AARHSFNEPH ILAIAQAIAE ERAKNGITGP CYVGKDTHAL SEPAFISVLE
    110 120 130 140 150
    VLAANGVDVI VQENNGFTPT PAVSNAILVH NKKGGPLADG IVITPSHNPP
    160 170 180 190 200
    EDGGIKYNPP NGGPADTNVT KVVEDRANAL LADGLKGVKR ISLDEAMASG
    210 220 230 240 250
    HVKEQDLVQP FVEGLADIVD MAAIQKAGLT LGVDPLGGSG IEYWKRIGEY
    260 270 280 290 300
    YNLNLTIVND QVDQTFRFMH LDKDGAIRMD CSSECAMAGL LALRDKFDLA
    310 320 330 340 350
    FANDPDYDRH GIVTPAGLMN PNHYLAVAIN YLFQHRPQWG KDVAVGKTLV
    360 370 380 390 400
    SSAMIDRVVN DLGRKLVEVP VGFKWFVDGL FDGSFGFGGE ESAGASFLRF
    410 420 430 440 450
    DGTPWSTDKD GIIMCLLAAE ITAVTGKNPQ EHYNELAKRF GAPSYNRLQA
    460 470 480 490 500
    AATSAQKAAL SKLSPEMVSA STLAGDPITA RLTAAPGNGA SIGGLKVMTD
    510 520 530 540
    NGWFAARPSG TEDAYKIYCE SFLGEEHRKQ IEKEAVEIVS EVLKNA
    Length:546
    Mass (Da):58,361
    Last modified:June 1, 1994 - v1
    Checksum:i666B6B9C2F2ECD59
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U08369 Genomic DNA. Translation: AAA57067.1.
    U00096 Genomic DNA. Translation: AAC73782.1.
    AP009048 Genomic DNA. Translation: BAA35337.1.
    U07651 Unassigned RNA. No translation available.
    PIRiG64803.
    RefSeqiNP_415214.1. NC_000913.3.
    WP_001297249.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73782; AAC73782; b0688.
    BAA35337; BAA35337; BAA35337.
    GeneIDi945271.
    KEGGiecj:JW0675.
    eco:b0688.
    PATRICi32116569. VBIEscCol129921_0717.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U08369 Genomic DNA. Translation: AAA57067.1.
    U00096 Genomic DNA. Translation: AAC73782.1.
    AP009048 Genomic DNA. Translation: BAA35337.1.
    U07651 Unassigned RNA. No translation available.
    PIRiG64803.
    RefSeqiNP_415214.1. NC_000913.3.
    WP_001297249.1. NZ_LN832404.1.

    3D structure databases

    ProteinModelPortaliP36938.
    SMRiP36938. Positions 3-545.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4261910. 16 interactions.
    DIPiDIP-10472N.
    IntActiP36938. 7 interactions.
    MINTiMINT-1240111.
    STRINGi511145.b0688.

    Proteomic databases

    EPDiP36938.
    PaxDbiP36938.
    PRIDEiP36938.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC73782; AAC73782; b0688.
    BAA35337; BAA35337; BAA35337.
    GeneIDi945271.
    KEGGiecj:JW0675.
    eco:b0688.
    PATRICi32116569. VBIEscCol129921_0717.

    Organism-specific databases

    EchoBASEiEB2065.
    EcoGeneiEG12144. pgm.

    Phylogenomic databases

    eggNOGiENOG4107QIX. Bacteria.
    COG0033. LUCA.
    HOGENOMiHOG000268677.
    InParanoidiP36938.
    KOiK01835.
    OMAiYYARHDY.
    PhylomeDBiP36938.

    Enzyme and pathway databases

    BioCyciEcoCyc:PHOSPHOGLUCMUT-MONOMER.
    ECOL316407:JW0675-MONOMER.
    MetaCyc:PHOSPHOGLUCMUT-MONOMER.

    Miscellaneous databases

    PROiP36938.

    Family and domain databases

    Gene3Di3.30.310.50. 1 hit.
    3.40.120.10. 3 hits.
    InterProiIPR005844. A-D-PHexomutase_a/b/a-I.
    IPR016055. A-D-PHexomutase_a/b/a-I/II/III.
    IPR005845. A-D-PHexomutase_a/b/a-II.
    IPR005846. A-D-PHexomutase_a/b/a-III.
    IPR005843. A-D-PHexomutase_C.
    IPR016066. A-D-PHexomutase_CS.
    IPR005852. PGlcMutase_a-D-Glc-sp.
    [Graphical view]
    PfamiPF02878. PGM_PMM_I. 1 hit.
    PF02879. PGM_PMM_II. 1 hit.
    PF02880. PGM_PMM_III. 1 hit.
    PF00408. PGM_PMM_IV. 1 hit.
    [Graphical view]
    SUPFAMiSSF53738. SSF53738. 3 hits.
    SSF55957. SSF55957. 1 hit.
    TIGRFAMsiTIGR01132. pgm. 1 hit.
    PROSITEiPS00710. PGM_PMM. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPGM_ECOLI
    AccessioniPrimary (citable) accession number: P36938
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: September 7, 2016
    This is version 139 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.