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Protein

Phosphoglucomutase

Gene

pgm

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This enzyme participates in both the breakdown and synthesis of glucose.1 Publication

Catalytic activityi

Alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate.1 Publication

Cofactori

Mg2+1 PublicationNote: Binds 1 Mg2+ ion per subunit.1 Publication

Kineticsi

  1. KM=50 µM for D-glucose-1-phosphate1 Publication

    pH dependencei

    Optimum pH is 9.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei146Phosphoserine intermediateBy similarity1
    Metal bindingi146Magnesium; via phosphate groupBy similarity1
    Binding sitei156SubstrateBy similarity1
    Metal bindingi304MagnesiumBy similarity1
    Metal bindingi306MagnesiumBy similarity1
    Metal bindingi308MagnesiumBy similarity1
    Binding sitei409SubstrateBy similarity1

    GO - Molecular functioni

    • magnesium ion binding Source: InterPro
    • phosphoglucomutase activity Source: EcoCyc

    GO - Biological processi

    Keywordsi

    Molecular functionIsomerase
    Biological processCarbohydrate metabolism, Glucose metabolism
    LigandMagnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:PHOSPHOGLUCMUT-MONOMER
    MetaCyc:PHOSPHOGLUCMUT-MONOMER

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoglucomutase (EC:5.4.2.2)
    Short name:
    PGM
    Alternative name(s):
    Glucose phosphomutase
    Gene namesi
    Name:pgm
    Ordered Locus Names:b0688, JW0675
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG12144 pgm

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: EcoCyc

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001478091 – 546PhosphoglucomutaseAdd BLAST546

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    EPDiP36938
    PaxDbiP36938
    PRIDEiP36938

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ghrBP376662EBI-542427,EBI-562547

    Protein-protein interaction databases

    BioGridi4261910, 31 interactors
    DIPiDIP-10472N
    IntActiP36938, 9 interactors
    STRINGi316385.ECDH10B_0753

    Structurei

    3D structure databases

    ProteinModelPortaliP36938
    SMRiP36938
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni146 – 147Substrate bindingBy similarity2
    Regioni308 – 309Substrate bindingBy similarity2
    Regioni390 – 392Substrate bindingBy similarity3

    Sequence similaritiesi

    Belongs to the phosphohexose mutase family.Curated

    Phylogenomic databases

    eggNOGiENOG4107QIX Bacteria
    COG0033 LUCA
    HOGENOMiHOG000268677
    InParanoidiP36938
    KOiK01835
    OMAiDIYKIYA
    PhylomeDBiP36938

    Family and domain databases

    CDDicd05801 PGM_like3, 1 hit
    InterProiView protein in InterPro
    IPR005844 A-D-PHexomutase_a/b/a-I
    IPR016055 A-D-PHexomutase_a/b/a-I/II/III
    IPR005845 A-D-PHexomutase_a/b/a-II
    IPR005846 A-D-PHexomutase_a/b/a-III
    IPR005843 A-D-PHexomutase_C
    IPR036900 A-D-PHexomutase_C_sf
    IPR016066 A-D-PHexomutase_CS
    IPR005852 PGlcMutase_a-D-Glc-sp
    PfamiView protein in Pfam
    PF02878 PGM_PMM_I, 1 hit
    PF02879 PGM_PMM_II, 1 hit
    PF02880 PGM_PMM_III, 1 hit
    PF00408 PGM_PMM_IV, 1 hit
    SUPFAMiSSF53738 SSF53738, 3 hits
    SSF55957 SSF55957, 1 hit
    TIGRFAMsiTIGR01132 pgm, 1 hit
    PROSITEiView protein in PROSITE
    PS00710 PGM_PMM, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P36938-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAIHNRAGQP AQQSDLINVA QLTAQYYVLK PEAGNAEHAV KFGTSGHRGS
    60 70 80 90 100
    AARHSFNEPH ILAIAQAIAE ERAKNGITGP CYVGKDTHAL SEPAFISVLE
    110 120 130 140 150
    VLAANGVDVI VQENNGFTPT PAVSNAILVH NKKGGPLADG IVITPSHNPP
    160 170 180 190 200
    EDGGIKYNPP NGGPADTNVT KVVEDRANAL LADGLKGVKR ISLDEAMASG
    210 220 230 240 250
    HVKEQDLVQP FVEGLADIVD MAAIQKAGLT LGVDPLGGSG IEYWKRIGEY
    260 270 280 290 300
    YNLNLTIVND QVDQTFRFMH LDKDGAIRMD CSSECAMAGL LALRDKFDLA
    310 320 330 340 350
    FANDPDYDRH GIVTPAGLMN PNHYLAVAIN YLFQHRPQWG KDVAVGKTLV
    360 370 380 390 400
    SSAMIDRVVN DLGRKLVEVP VGFKWFVDGL FDGSFGFGGE ESAGASFLRF
    410 420 430 440 450
    DGTPWSTDKD GIIMCLLAAE ITAVTGKNPQ EHYNELAKRF GAPSYNRLQA
    460 470 480 490 500
    AATSAQKAAL SKLSPEMVSA STLAGDPITA RLTAAPGNGA SIGGLKVMTD
    510 520 530 540
    NGWFAARPSG TEDAYKIYCE SFLGEEHRKQ IEKEAVEIVS EVLKNA
    Length:546
    Mass (Da):58,361
    Last modified:June 1, 1994 - v1
    Checksum:i666B6B9C2F2ECD59
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U08369 Genomic DNA Translation: AAA57067.1
    U00096 Genomic DNA Translation: AAC73782.1
    AP009048 Genomic DNA Translation: BAA35337.1
    U07651 Unassigned RNA No translation available.
    PIRiG64803
    RefSeqiNP_415214.1, NC_000913.3
    WP_001297249.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC73782; AAC73782; b0688
    BAA35337; BAA35337; BAA35337
    GeneIDi945271
    KEGGiecj:JW0675
    eco:b0688
    PATRICifig|1411691.4.peg.1588

    Similar proteinsi

    Entry informationi

    Entry nameiPGM_ECOLI
    AccessioniPrimary (citable) accession number: P36938
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: March 28, 2018
    This is version 151 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families
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