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Protein

Ribosomal RNA small subunit methyltransferase B

Gene

rsmB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + cytosine(967) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(967) in 16S rRNA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei277S-adenosyl-L-methionine1 Publication1
Binding sitei303S-adenosyl-L-methionine1 Publication1
Binding sitei322S-adenosyl-L-methionine1 Publication1
Active sitei375NucleophileBy similarity1

GO - Molecular functioni

  • RNA binding Source: UniProtKB-KW
  • rRNA (cytosine-C5-)-methyltransferase activity Source: EcoCyc

GO - Biological processi

  • regulation of transcription, DNA-templated Source: InterPro
  • rRNA base methylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG12163-MONOMER.
ECOL316407:JW3250-MONOMER.
MetaCyc:EG12163-MONOMER.
BRENDAi2.1.1.176. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase B (EC:2.1.1.176)
Alternative name(s):
16S rRNA m5C967 methyltransferase
rRNA (cytosine-C(5)-)-methyltransferase RsmB
Gene namesi
Name:rsmB
Synonyms:fmu/fmv, rrmB, sun, yhdB
Ordered Locus Names:b3289, JW3250
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12163. rsmB.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi325C → A: Reduces activity 3-fold. 2 Publications1
Mutagenesisi375C → A: Loss of activity. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002117921 – 429Ribosomal RNA small subunit methyltransferase BAdd BLAST429

Proteomic databases

PaxDbiP36929.
PRIDEiP36929.

Interactioni

Protein-protein interaction databases

BioGridi4263436. 10 interactors.
DIPiDIP-10946N.
IntActiP36929. 14 interactors.
STRINGi511145.b3289.

Structurei

Secondary structure

1429
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 21Combined sources15
Helixi25 – 33Combined sources9
Helixi38 – 65Combined sources28
Helixi72 – 74Combined sources3
Helixi75 – 90Combined sources16
Helixi95 – 108Combined sources14
Helixi112 – 114Combined sources3
Helixi115 – 136Combined sources22
Helixi140 – 143Combined sources4
Helixi147 – 156Combined sources10
Turni158 – 160Combined sources3
Helixi161 – 168Combined sources8
Beta strandi174 – 178Combined sources5
Turni180 – 182Combined sources3
Helixi185 – 194Combined sources10
Beta strandi208 – 213Combined sources6
Helixi217 – 219Combined sources3
Helixi223 – 225Combined sources3
Beta strandi227 – 230Combined sources4
Helixi233 – 236Combined sources4
Helixi238 – 242Combined sources5
Beta strandi249 – 254Combined sources6
Helixi259 – 267Combined sources9
Beta strandi272 – 279Combined sources8
Turni280 – 282Combined sources3
Helixi283 – 292Combined sources10
Beta strandi298 – 301Combined sources4
Helixi308 – 311Combined sources4
Beta strandi316 – 322Combined sources7
Helixi328 – 330Combined sources3
Turni331 – 333Combined sources3
Helixi337 – 340Combined sources4
Helixi345 – 360Combined sources16
Helixi361 – 363Combined sources3
Beta strandi364 – 375Combined sources12
Turni379 – 382Combined sources4
Helixi383 – 392Combined sources10
Beta strandi400 – 402Combined sources3
Beta strandi404 – 411Combined sources8
Beta strandi420 – 427Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J4Fmodel-A228-429[»]
1SQFX-ray2.10A1-429[»]
1SQGX-ray1.65A1-429[»]
ProteinModelPortaliP36929.
SMRiP36929.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP36929.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni254 – 260S-adenosyl-L-methionine binding7

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CYJ. Bacteria.
COG0144. LUCA.
HOGENOMiHOG000037300.
InParanoidiP36929.
KOiK03500.
OMAiLRVNRQH.
PhylomeDBiP36929.

Family and domain databases

Gene3Di1.10.940.10. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_01856. 16SrRNA_methyltr_B. 1 hit.
InterProiIPR018314. Fmu/NOL1/Nop2p_CS.
IPR001678. MeTrfase_RsmB/NOP2.
IPR006027. NusB_RsmB_TIM44.
IPR023267. RCMT.
IPR004573. rRNA_ssu_MeTfrase_B.
IPR023541. rRNA_ssu_MeTfrase_B_ent.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
PF01029. NusB. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF48013. SSF48013. 1 hit.
SSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00563. rsmB. 1 hit.
PROSITEiPS01153. NOL1_NOP2_SUN. 1 hit.
PS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36929-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKQRNLRSM AAQAVEQVVE QGQSLSNILP PLQQKVSDKD KALLQELCFG
60 70 80 90 100
VLRTLSQLDW LINKLMARPM TGKQRTVHYL IMVGLYQLLY TRIPPHAALA
110 120 130 140 150
ETVEGAIAIK RPQLKGLING VLRQFQRQQE ELLAEFNASD ARYLHPSWLL
160 170 180 190 200
KRLQKAYPEQ WQSIVEANNQ RPPMWLRINR THHSRDSWLA LLDEAGMKGF
210 220 230 240 250
PHADYPDAVR LETPAPVHAL PGFEDGWVTV QDASAQGCMT WLAPQNGEHI
260 270 280 290 300
LDLCAAPGGK TTHILEVAPE AQVVAVDIDE QRLSRVYDNL KRLGMKATVK
310 320 330 340 350
QGDGRYPSQW CGEQQFDRIL LDAPCSATGV IRRHPDIKWL RRDRDIPELA
360 370 380 390 400
QLQSEILDAI WPHLKTGGTL VYATCSVLPE ENSLQIKAFL QRTADAELCE
410 420
TGTPEQPGKQ NLPGAEEGDG FFYAKLIKK
Length:429
Mass (Da):48,348
Last modified:November 1, 1995 - v2
Checksum:i1F516223C84D80F8
GO

Sequence cautioni

The sequence AAA58086 differs from that shown. Reason: Frameshift at position 384.Curated
The sequence CAA54369 differs from that shown. Reason: Frameshift at position 201. Was originally named Fmu and represented the N-terminal part of the RsmB protein.Curated
The sequence CAA54370 differs from that shown. Reason: Frameshift at position 201. Was originally named Fmv and represented the C-terminal part of the RsmB protein.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3K → C AA sequence (PubMed:10026269).Curated1
Sequence conflicti3K → W AA sequence (PubMed:10026269).Curated1
Sequence conflicti105 – 106GA → RR in CAA54369 (PubMed:8432722).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77091 Genomic DNA. Translation: CAA54369.1. Frameshift.
X77091 Genomic DNA. Translation: CAA54370.1. Frameshift.
Y10307 Genomic DNA. Translation: CAA71359.1.
U18997 Genomic DNA. Translation: AAA58086.1. Frameshift.
U00096 Genomic DNA. Translation: AAC76314.1.
AP009048 Genomic DNA. Translation: BAE78003.1.
X52114 Genomic DNA. Translation: CAA36358.1.
PIRiD65121.
RefSeqiNP_417747.1. NC_000913.3.
WP_000744778.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76314; AAC76314; b3289.
BAE78003; BAE78003; BAE78003.
GeneIDi947789.
KEGGiecj:JW3250.
eco:b3289.
PATRICi32122010. VBIEscCol129921_3382.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77091 Genomic DNA. Translation: CAA54369.1. Frameshift.
X77091 Genomic DNA. Translation: CAA54370.1. Frameshift.
Y10307 Genomic DNA. Translation: CAA71359.1.
U18997 Genomic DNA. Translation: AAA58086.1. Frameshift.
U00096 Genomic DNA. Translation: AAC76314.1.
AP009048 Genomic DNA. Translation: BAE78003.1.
X52114 Genomic DNA. Translation: CAA36358.1.
PIRiD65121.
RefSeqiNP_417747.1. NC_000913.3.
WP_000744778.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J4Fmodel-A228-429[»]
1SQFX-ray2.10A1-429[»]
1SQGX-ray1.65A1-429[»]
ProteinModelPortaliP36929.
SMRiP36929.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263436. 10 interactors.
DIPiDIP-10946N.
IntActiP36929. 14 interactors.
STRINGi511145.b3289.

Proteomic databases

PaxDbiP36929.
PRIDEiP36929.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76314; AAC76314; b3289.
BAE78003; BAE78003; BAE78003.
GeneIDi947789.
KEGGiecj:JW3250.
eco:b3289.
PATRICi32122010. VBIEscCol129921_3382.

Organism-specific databases

EchoBASEiEB2082.
EcoGeneiEG12163. rsmB.

Phylogenomic databases

eggNOGiENOG4105CYJ. Bacteria.
COG0144. LUCA.
HOGENOMiHOG000037300.
InParanoidiP36929.
KOiK03500.
OMAiLRVNRQH.
PhylomeDBiP36929.

Enzyme and pathway databases

BioCyciEcoCyc:EG12163-MONOMER.
ECOL316407:JW3250-MONOMER.
MetaCyc:EG12163-MONOMER.
BRENDAi2.1.1.176. 2026.

Miscellaneous databases

EvolutionaryTraceiP36929.
PROiP36929.

Family and domain databases

Gene3Di1.10.940.10. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_01856. 16SrRNA_methyltr_B. 1 hit.
InterProiIPR018314. Fmu/NOL1/Nop2p_CS.
IPR001678. MeTrfase_RsmB/NOP2.
IPR006027. NusB_RsmB_TIM44.
IPR023267. RCMT.
IPR004573. rRNA_ssu_MeTfrase_B.
IPR023541. rRNA_ssu_MeTfrase_B_ent.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
PF01029. NusB. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF48013. SSF48013. 1 hit.
SSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00563. rsmB. 1 hit.
PROSITEiPS01153. NOL1_NOP2_SUN. 1 hit.
PS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRSMB_ECOLI
AccessioniPrimary (citable) accession number: P36929
Secondary accession number(s): P23866, Q2M6V3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 152 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.