Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ribosomal RNA small subunit methyltransferase B

Gene

rsmB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + cytosine(967) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(967) in 16S rRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei277 – 2771S-adenosyl-L-methionine1 Publication
Binding sitei303 – 3031S-adenosyl-L-methionine1 Publication
Binding sitei322 – 3221S-adenosyl-L-methionine1 Publication
Active sitei375 – 3751NucleophileBy similarity

GO - Molecular functioni

  • RNA binding Source: UniProtKB-KW
  • rRNA (cytosine-C5-)-methyltransferase activity Source: EcoCyc

GO - Biological processi

  • regulation of transcription, DNA-templated Source: InterPro
  • rRNA base methylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG12163-MONOMER.
ECOL316407:JW3250-MONOMER.
MetaCyc:EG12163-MONOMER.
BRENDAi2.1.1.176. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase B (EC:2.1.1.176)
Alternative name(s):
16S rRNA m5C967 methyltransferase
rRNA (cytosine-C(5)-)-methyltransferase RsmB
Gene namesi
Name:rsmB
Synonyms:fmu/fmv, rrmB, sun, yhdB
Ordered Locus Names:b3289, JW3250
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12163. rsmB.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi325 – 3251C → A: Reduces activity 3-fold. 2 Publications
Mutagenesisi375 – 3751C → A: Loss of activity. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 429429Ribosomal RNA small subunit methyltransferase BPRO_0000211792Add
BLAST

Proteomic databases

PaxDbiP36929.
PRIDEiP36929.

Interactioni

Protein-protein interaction databases

BioGridi4263436. 10 interactions.
DIPiDIP-10946N.
IntActiP36929. 14 interactions.
STRINGi511145.b3289.

Structurei

Secondary structure

1
429
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 2115Combined sources
Helixi25 – 339Combined sources
Helixi38 – 6528Combined sources
Helixi72 – 743Combined sources
Helixi75 – 9016Combined sources
Helixi95 – 10814Combined sources
Helixi112 – 1143Combined sources
Helixi115 – 13622Combined sources
Helixi140 – 1434Combined sources
Helixi147 – 15610Combined sources
Turni158 – 1603Combined sources
Helixi161 – 1688Combined sources
Beta strandi174 – 1785Combined sources
Turni180 – 1823Combined sources
Helixi185 – 19410Combined sources
Beta strandi208 – 2136Combined sources
Helixi217 – 2193Combined sources
Helixi223 – 2253Combined sources
Beta strandi227 – 2304Combined sources
Helixi233 – 2364Combined sources
Helixi238 – 2425Combined sources
Beta strandi249 – 2546Combined sources
Helixi259 – 2679Combined sources
Beta strandi272 – 2798Combined sources
Turni280 – 2823Combined sources
Helixi283 – 29210Combined sources
Beta strandi298 – 3014Combined sources
Helixi308 – 3114Combined sources
Beta strandi316 – 3227Combined sources
Helixi328 – 3303Combined sources
Turni331 – 3333Combined sources
Helixi337 – 3404Combined sources
Helixi345 – 36016Combined sources
Helixi361 – 3633Combined sources
Beta strandi364 – 37512Combined sources
Turni379 – 3824Combined sources
Helixi383 – 39210Combined sources
Beta strandi400 – 4023Combined sources
Beta strandi404 – 4118Combined sources
Beta strandi420 – 4278Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J4Fmodel-A228-429[»]
1SQFX-ray2.10A1-429[»]
1SQGX-ray1.65A1-429[»]
ProteinModelPortaliP36929.
SMRiP36929. Positions 5-428.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP36929.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni254 – 2607S-adenosyl-L-methionine binding

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CYJ. Bacteria.
COG0144. LUCA.
HOGENOMiHOG000037300.
InParanoidiP36929.
KOiK03500.
OMAiLRVNRQH.
PhylomeDBiP36929.

Family and domain databases

Gene3Di1.10.940.10. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_01856. 16SrRNA_methyltr_B. 1 hit.
InterProiIPR018314. Fmu/NOL1/Nop2p_CS.
IPR001678. MeTrfase_RsmB/NOP2.
IPR006027. NusB_RsmB_TIM44.
IPR023267. RCMT.
IPR004573. rRNA_ssu_MeTfrase_B.
IPR023541. rRNA_ssu_MeTfrase_B_ent.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
PF01029. NusB. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF48013. SSF48013. 1 hit.
SSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00563. rsmB. 1 hit.
PROSITEiPS01153. NOL1_NOP2_SUN. 1 hit.
PS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36929-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKQRNLRSM AAQAVEQVVE QGQSLSNILP PLQQKVSDKD KALLQELCFG
60 70 80 90 100
VLRTLSQLDW LINKLMARPM TGKQRTVHYL IMVGLYQLLY TRIPPHAALA
110 120 130 140 150
ETVEGAIAIK RPQLKGLING VLRQFQRQQE ELLAEFNASD ARYLHPSWLL
160 170 180 190 200
KRLQKAYPEQ WQSIVEANNQ RPPMWLRINR THHSRDSWLA LLDEAGMKGF
210 220 230 240 250
PHADYPDAVR LETPAPVHAL PGFEDGWVTV QDASAQGCMT WLAPQNGEHI
260 270 280 290 300
LDLCAAPGGK TTHILEVAPE AQVVAVDIDE QRLSRVYDNL KRLGMKATVK
310 320 330 340 350
QGDGRYPSQW CGEQQFDRIL LDAPCSATGV IRRHPDIKWL RRDRDIPELA
360 370 380 390 400
QLQSEILDAI WPHLKTGGTL VYATCSVLPE ENSLQIKAFL QRTADAELCE
410 420
TGTPEQPGKQ NLPGAEEGDG FFYAKLIKK
Length:429
Mass (Da):48,348
Last modified:November 1, 1995 - v2
Checksum:i1F516223C84D80F8
GO

Sequence cautioni

The sequence AAA58086 differs from that shown. Reason: Frameshift at position 384. Curated
The sequence CAA54369 differs from that shown. Reason: Frameshift at position 201. Was originally named Fmu and represented the N-terminal part of the RsmB protein.Curated
The sequence CAA54370 differs from that shown. Reason: Frameshift at position 201. Was originally named Fmv and represented the C-terminal part of the RsmB protein.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti3 – 31K → C AA sequence (PubMed:10026269).Curated
Sequence conflicti3 – 31K → W AA sequence (PubMed:10026269).Curated
Sequence conflicti105 – 1062GA → RR in CAA54369 (PubMed:8432722).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77091 Genomic DNA. Translation: CAA54369.1. Frameshift.
X77091 Genomic DNA. Translation: CAA54370.1. Frameshift.
Y10307 Genomic DNA. Translation: CAA71359.1.
U18997 Genomic DNA. Translation: AAA58086.1. Frameshift.
U00096 Genomic DNA. Translation: AAC76314.1.
AP009048 Genomic DNA. Translation: BAE78003.1.
X52114 Genomic DNA. Translation: CAA36358.1.
PIRiD65121.
RefSeqiNP_417747.1. NC_000913.3.
WP_000744778.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76314; AAC76314; b3289.
BAE78003; BAE78003; BAE78003.
GeneIDi947789.
KEGGiecj:JW3250.
eco:b3289.
PATRICi32122010. VBIEscCol129921_3382.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77091 Genomic DNA. Translation: CAA54369.1. Frameshift.
X77091 Genomic DNA. Translation: CAA54370.1. Frameshift.
Y10307 Genomic DNA. Translation: CAA71359.1.
U18997 Genomic DNA. Translation: AAA58086.1. Frameshift.
U00096 Genomic DNA. Translation: AAC76314.1.
AP009048 Genomic DNA. Translation: BAE78003.1.
X52114 Genomic DNA. Translation: CAA36358.1.
PIRiD65121.
RefSeqiNP_417747.1. NC_000913.3.
WP_000744778.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J4Fmodel-A228-429[»]
1SQFX-ray2.10A1-429[»]
1SQGX-ray1.65A1-429[»]
ProteinModelPortaliP36929.
SMRiP36929. Positions 5-428.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263436. 10 interactions.
DIPiDIP-10946N.
IntActiP36929. 14 interactions.
STRINGi511145.b3289.

Proteomic databases

PaxDbiP36929.
PRIDEiP36929.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76314; AAC76314; b3289.
BAE78003; BAE78003; BAE78003.
GeneIDi947789.
KEGGiecj:JW3250.
eco:b3289.
PATRICi32122010. VBIEscCol129921_3382.

Organism-specific databases

EchoBASEiEB2082.
EcoGeneiEG12163. rsmB.

Phylogenomic databases

eggNOGiENOG4105CYJ. Bacteria.
COG0144. LUCA.
HOGENOMiHOG000037300.
InParanoidiP36929.
KOiK03500.
OMAiLRVNRQH.
PhylomeDBiP36929.

Enzyme and pathway databases

BioCyciEcoCyc:EG12163-MONOMER.
ECOL316407:JW3250-MONOMER.
MetaCyc:EG12163-MONOMER.
BRENDAi2.1.1.176. 2026.

Miscellaneous databases

EvolutionaryTraceiP36929.
PROiP36929.

Family and domain databases

Gene3Di1.10.940.10. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_01856. 16SrRNA_methyltr_B. 1 hit.
InterProiIPR018314. Fmu/NOL1/Nop2p_CS.
IPR001678. MeTrfase_RsmB/NOP2.
IPR006027. NusB_RsmB_TIM44.
IPR023267. RCMT.
IPR004573. rRNA_ssu_MeTfrase_B.
IPR023541. rRNA_ssu_MeTfrase_B_ent.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
PF01029. NusB. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF48013. SSF48013. 1 hit.
SSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00563. rsmB. 1 hit.
PROSITEiPS01153. NOL1_NOP2_SUN. 1 hit.
PS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRSMB_ECOLI
AccessioniPrimary (citable) accession number: P36929
Secondary accession number(s): P23866, Q2M6V3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: November 1, 1995
Last modified: September 7, 2016
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.