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Protein

Endo-1,4-beta-xylanase A

Gene

xynA

Organism
Thermoanaerobacterium saccharolyticum
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Endo-acting enzyme that randomly cleaves the internal xylosidic linkages of the xylan backbone, yielding xylooligosaccharides of various lengths which are further hydrolyzed to xylose molecules by beta-xylosidase (EC 3.2.1.37). Requires at least three xylose residues for catalytic activity. Does not have activity against xylobiose.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.

pH dependencei

Optimum pH is 5.5.

Temperature dependencei

Optimum temperature is 70 degrees Celsius.

Pathwayi: xylan degradation

This protein is involved in the pathway xylan degradation, which is part of Glycan degradation.
View all proteins of this organism that are known to be involved in the pathway xylan degradation and in Glycan degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei495Proton donorBy similarity1
Active sitei5371 Publication1
Active sitei600NucleophilePROSITE-ProRule annotation1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Polysaccharide degradation, Xylan degradation

Enzyme and pathway databases

UniPathwayiUPA00114

Protein family/group databases

CAZyiCBM22 Carbohydrate-Binding Module Family 22
CBM9 Carbohydrate-Binding Module Family 9
GH10 Glycoside Hydrolase Family 10

Names & Taxonomyi

Protein namesi
Recommended name:
Endo-1,4-beta-xylanase A (EC:3.2.1.8)
Short name:
Xylanase A
Alternative name(s):
1,4-beta-D-xylan xylanohydrolase A
Gene namesi
Name:xynA
OrganismiThermoanaerobacterium saccharolyticum
Taxonomic identifieri28896 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacterales Family III. Incertae SedisThermoanaerobacterium

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi537D → N: Loss of activity. 1 Publication1
Mutagenesisi600E → Q: Loss of activity. 1 Publication1
Mutagenesisi602D → N: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 331 PublicationAdd BLAST33
ChainiPRO_000000798034 – 1157Endo-1,4-beta-xylanase AAdd BLAST1124

Proteomic databases

PRIDEiP36917

Expressioni

Inductioni

By xylan and xylose.

Structurei

3D structure databases

ProteinModelPortaliP36917
SMRiP36917
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 189CBM-cenC 1Add BLAST152
Domaini195 – 343CBM-cenC 2Add BLAST149
Domaini352 – 675GH10PROSITE-ProRule annotationAdd BLAST324
Domaini1051 – 1114SLH 1PROSITE-ProRule annotationAdd BLAST64
Domaini1115 – 1157SLH 2PROSITE-ProRule annotationAdd BLAST43

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.60.120.260, 2 hits
InterProiView protein in InterPro
IPR010502 Carb-bd_dom_fam9
IPR003305 CenC_carb-bd
IPR008979 Galactose-bd-like_sf
IPR001000 GH10
IPR031158 GH10_AS
IPR018087 Glyco_hydro_5_CS
IPR017853 Glycoside_hydrolase_SF
IPR001119 SLH_dom
PfamiView protein in Pfam
PF06452 CBM9_1, 2 hits
PF02018 CBM_4_9, 2 hits
PF00331 Glyco_hydro_10, 1 hit
PF00395 SLH, 2 hits
PRINTSiPR00134 GLHYDRLASE10
SMARTiView protein in SMART
SM00633 Glyco_10, 1 hit
SUPFAMiSSF49785 SSF49785, 2 hits
SSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS00591 GH10_1, 1 hit
PS51760 GH10_2, 1 hit
PS00659 GLYCOSYL_HYDROL_F5, 1 hit
PS51272 SLH, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36917-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMKNNVDRIV SIVTALIMIF GASLFSPPIR VFADDTNINL VSNGDFESGT
60 70 80 90 100
IDGWIKQGNP TLAVTTEQAI GQYSMKVTGR TQTYEGPAYS FLGKMQKGES
110 120 130 140 150
YSVSLKVRLV SGQNSSNPLI TVTMFREDDN GKHYDTIVWQ KQVSEDSWTT
160 170 180 190 200
VSGTYTLDYI GTLKTLYMYV ESPDPTLEYY IDDVVVTTQN PIQVGNVIAN
210 220 230 240 250
ETFENGNTSG WIGTGSSVVK AVYGVAHSGD YSLLTTGRTA NWNGPSYDLT
260 270 280 290 300
GKIVPGQQYN VDFWVKFVNG NDTEQIKATV KATSDKDNYI QVNDFANVNK
310 320 330 340 350
GEWTEIKGSF TLPVADYSGI SIYVESQNPT LEFYIDDFSV IGEISNNQIT
360 370 380 390 400
IQNDIPDLYS VFKDYFPIGV AVDPSRLNDA DPHAQLTAKH FNMLVAENAM
410 420 430 440 450
KPESLQPTEG NFTFDNADKI VDYAIAHNMK MRGHTLLWHN QVPDWFFQDP
460 470 480 490 500
SDPSKSASRD LLLQRLKTHI TTVLDHFKTK YGSQNPIIGW DVVNEVLDDN
510 520 530 540 550
GNLRNSKWLQ IIGPDYIEKA FEYAHEADPS MKLFINDYNI ENNGVKTQAM
560 570 580 590 600
YDLVKKLKSE GVPIDGIGMQ MHININSNID NIKASIEKLA SLGVEIQVTE
610 620 630 640 650
LDMNMNGNIS NEALLKQARL YKQLFDLFKA EKQYITAVVF WGVSDDVTWL
660 670 680 690 700
SKPNAPLLFD SKLQAKPAFW AVVDPSKAIP DIQSAKALEG SPTIGANVDS
710 720 730 740 750
SWKLVKPLYV NTYVEGTVGA TATVKSMWDT KNLYLLVQVS DNTPSNNDGI
760 770 780 790 800
EIFVDKNDDK STSYETDDER YTIKRDGTGS SDITKYVTSN ADGYVAQLAI
810 820 830 840 850
PIEDISPAVN DKIGFDIRIN DDKGNGKIDA ITVWNDYTNS QNTNTSYFGD
860 870 880 890 900
IVLSKSAQIA TAIYGTPVID GKVDDIWNNV EPISTNTWIL GSNGATATQK
910 920 930 940 950
MMWDDKYLYV LADVTDSNLN KSSINPYEQD SVEVFVDQNN DKTTYYENDD
960 970 980 990 1000
GQYRVNYDNE QSFGGSTNSN GFKSATSLTQ SGYIVEEAIP WTSITPSNGT
1010 1020 1030 1040 1050
IIGFDLQVNN ADENGKRTGI VTWCDPSGNS WQDTSGFGNL LLTGKPSGAL
1060 1070 1080 1090 1100
KKGVTFDDIK NSWAKDAIEV LASRHIVEGM TDTQYEPNKT VTRAEFTAMI
1110 1120 1130 1140 1150
LRLLNIKEEQ YSGEFSDVNS GDWYANAIEA AYKAGIIEGD GKNARPNDSI

TREEMTQ
Length:1,157
Mass (Da):128,380
Last modified:June 1, 1994 - v1
Checksum:i51FA6004497EC58B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97882 Genomic DNA Translation: AAA21812.1 Sequence problems.
PIRiA48490

Similar proteinsi

Entry informationi

Entry nameiXYNA_THESA
AccessioniPrimary (citable) accession number: P36917
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 23, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

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