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P36917

- XYNA_THESA

UniProt

P36917 - XYNA_THESA

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Protein
Endo-1,4-beta-xylanase A
Gene
xynA
Organism
Thermoanaerobacterium saccharolyticum
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Endo-acting enzyme that randomly cleaves the internal xylosidic linkages of the xylan backbone, yielding xylooligosaccharides of various lengths which are further hydrolyzed to xylose molecules by beta-xylosidase (EC 3.2.1.37). Requires at least three xylose residues for catalytic activity. Does not have activity against xylobiose.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.

pH dependencei

Optimum pH is 5.5.

Temperature dependencei

Optimum temperature is 70 degrees Celsius.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei495 – 4951Proton donor By similarity
Active sitei537 – 53711 Publication
Active sitei600 – 6001Nucleophile1 Publication

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. endo-1,4-beta-xylanase activity Source: UniProtKB-EC

GO - Biological processi

  1. xylan catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation, Xylan degradation

Enzyme and pathway databases

UniPathwayiUPA00114.

Protein family/group databases

CAZyiCBM22. Carbohydrate-Binding Module Family 22.
CBM9. Carbohydrate-Binding Module Family 9.
GH10. Glycoside Hydrolase Family 10.

Names & Taxonomyi

Protein namesi
Recommended name:
Endo-1,4-beta-xylanase A (EC:3.2.1.8)
Short name:
Xylanase A
Alternative name(s):
1,4-beta-D-xylan xylanohydrolase A
Gene namesi
Name:xynA
OrganismiThermoanaerobacterium saccharolyticum
Taxonomic identifieri28896 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacterales Family III. Incertae SedisThermoanaerobacterium

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi537 – 5371D → N: Loss of activity.
Mutagenesisi600 – 6001E → Q: Loss of activity.
Mutagenesisi602 – 6021D → N: Loss of activity.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 33331 Publication
Add
BLAST
Chaini34 – 11571124Endo-1,4-beta-xylanase A
PRO_0000007980Add
BLAST

Expressioni

Inductioni

By xylan and xylose.

Structurei

3D structure databases

ProteinModelPortaliP36917.
SMRiP36917. Positions 859-1043.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 189152CBM-cenC 1
Add
BLAST
Domaini195 – 343149CBM-cenC 2
Add
BLAST
Domaini1051 – 111464SLH 1
Add
BLAST
Domaini1115 – 115743SLH 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni413 – 649237Catalytic Reviewed prediction
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
2.60.40.1190. 2 hits.
3.20.20.80. 1 hit.
InterProiIPR010502. Carb-bd_dom_fam9.
IPR003305. CenC_carb-bd.
IPR008979. Galactose-bd-like.
IPR001000. Glyco_hydro_10.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR001119. S-layer_homology_dom.
[Graphical view]
PfamiPF02018. CBM_4_9. 2 hits.
PF06452. DUF1083. 2 hits.
PF00331. Glyco_hydro_10. 1 hit.
PF00395. SLH. 2 hits.
[Graphical view]
PRINTSiPR00134. GLHYDRLASE10.
SMARTiSM00633. Glyco_10. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00591. GLYCOSYL_HYDROL_F10. 1 hit.
PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
PS51272. SLH. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36917-1 [UniParc]FASTAAdd to Basket

« Hide

MMKNNVDRIV SIVTALIMIF GASLFSPPIR VFADDTNINL VSNGDFESGT     50
IDGWIKQGNP TLAVTTEQAI GQYSMKVTGR TQTYEGPAYS FLGKMQKGES 100
YSVSLKVRLV SGQNSSNPLI TVTMFREDDN GKHYDTIVWQ KQVSEDSWTT 150
VSGTYTLDYI GTLKTLYMYV ESPDPTLEYY IDDVVVTTQN PIQVGNVIAN 200
ETFENGNTSG WIGTGSSVVK AVYGVAHSGD YSLLTTGRTA NWNGPSYDLT 250
GKIVPGQQYN VDFWVKFVNG NDTEQIKATV KATSDKDNYI QVNDFANVNK 300
GEWTEIKGSF TLPVADYSGI SIYVESQNPT LEFYIDDFSV IGEISNNQIT 350
IQNDIPDLYS VFKDYFPIGV AVDPSRLNDA DPHAQLTAKH FNMLVAENAM 400
KPESLQPTEG NFTFDNADKI VDYAIAHNMK MRGHTLLWHN QVPDWFFQDP 450
SDPSKSASRD LLLQRLKTHI TTVLDHFKTK YGSQNPIIGW DVVNEVLDDN 500
GNLRNSKWLQ IIGPDYIEKA FEYAHEADPS MKLFINDYNI ENNGVKTQAM 550
YDLVKKLKSE GVPIDGIGMQ MHININSNID NIKASIEKLA SLGVEIQVTE 600
LDMNMNGNIS NEALLKQARL YKQLFDLFKA EKQYITAVVF WGVSDDVTWL 650
SKPNAPLLFD SKLQAKPAFW AVVDPSKAIP DIQSAKALEG SPTIGANVDS 700
SWKLVKPLYV NTYVEGTVGA TATVKSMWDT KNLYLLVQVS DNTPSNNDGI 750
EIFVDKNDDK STSYETDDER YTIKRDGTGS SDITKYVTSN ADGYVAQLAI 800
PIEDISPAVN DKIGFDIRIN DDKGNGKIDA ITVWNDYTNS QNTNTSYFGD 850
IVLSKSAQIA TAIYGTPVID GKVDDIWNNV EPISTNTWIL GSNGATATQK 900
MMWDDKYLYV LADVTDSNLN KSSINPYEQD SVEVFVDQNN DKTTYYENDD 950
GQYRVNYDNE QSFGGSTNSN GFKSATSLTQ SGYIVEEAIP WTSITPSNGT 1000
IIGFDLQVNN ADENGKRTGI VTWCDPSGNS WQDTSGFGNL LLTGKPSGAL 1050
KKGVTFDDIK NSWAKDAIEV LASRHIVEGM TDTQYEPNKT VTRAEFTAMI 1100
LRLLNIKEEQ YSGEFSDVNS GDWYANAIEA AYKAGIIEGD GKNARPNDSI 1150
TREEMTQ 1157
Length:1,157
Mass (Da):128,380
Last modified:June 1, 1994 - v1
Checksum:i51FA6004497EC58B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M97882 Genomic DNA. Translation: AAA21812.1. Sequence problems.
PIRiA48490.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M97882 Genomic DNA. Translation: AAA21812.1 . Sequence problems.
PIRi A48490.

3D structure databases

ProteinModelPortali P36917.
SMRi P36917. Positions 859-1043.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM22. Carbohydrate-Binding Module Family 22.
CBM9. Carbohydrate-Binding Module Family 9.
GH10. Glycoside Hydrolase Family 10.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00114 .

Family and domain databases

Gene3Di 2.60.120.260. 2 hits.
2.60.40.1190. 2 hits.
3.20.20.80. 1 hit.
InterProi IPR010502. Carb-bd_dom_fam9.
IPR003305. CenC_carb-bd.
IPR008979. Galactose-bd-like.
IPR001000. Glyco_hydro_10.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR001119. S-layer_homology_dom.
[Graphical view ]
Pfami PF02018. CBM_4_9. 2 hits.
PF06452. DUF1083. 2 hits.
PF00331. Glyco_hydro_10. 1 hit.
PF00395. SLH. 2 hits.
[Graphical view ]
PRINTSi PR00134. GLHYDRLASE10.
SMARTi SM00633. Glyco_10. 1 hit.
[Graphical view ]
SUPFAMi SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEi PS00591. GLYCOSYL_HYDROL_F10. 1 hit.
PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
PS51272. SLH. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Gene cloning, sequencing, and biochemical characterization of endoxylanase from Thermoanaerobacterium saccharolyticum B6A-RI."
    Lee Y.-E., Lowe S.E., Zeikus J.G.
    Appl. Environ. Microbiol. 59:3134-3137(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 34-41.
    Strain: ATCC 49915 / DSM 7060 / B6A-RI.
  2. "Characterization of the active site and thermostability regions of endoxylanase from Thermoanaerobacterium saccharolyticum B6A-RI."
    Lee Y.-E., Lowe S.E., Henrissat B., Zeikus J.G.
    J. Bacteriol. 175:5890-5898(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACTIVE SITE, MUTAGENESIS.

Entry informationi

Entry nameiXYNA_THESA
AccessioniPrimary (citable) accession number: P36917
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: January 22, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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