Reviewed,
UniProtKB/Swiss-Prot P36914 (AMYG_ASPOR)
Last modified
September 22, 2009.
Version 67.
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Glucoamylase EC=3.2.1.3 Alternative name(s): Glucan 1,4-alpha-glucosidase 1,4-alpha-D-glucan glucohydrolase | ||||||
| Gene names |
| ||||||
| Organism | Aspergillus oryzae [Complete proteome] | ||||||
| Taxonomic identifier | 5062 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus |
Protein attributes
| Sequence length | 612 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Catalytic activity | Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose. |
| Sequence similarities | Belongs to the glycosyl hydrolase 15 family. Contains 1 CBM20 (carbohydrate binding type-20) domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Carbohydrate metabolism Polysaccharide degradation |
| Domain | Signal |
| Molecular function | Glycosidase Hydrolase |
| PTM | Disulfide bond Glycoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | carbohydrate binding Inferred from electronic annotation. Source: InterPro glucan 1,4-alpha-glucosidase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 19 | 19 | By similarity | ||||||||
| Propeptide | 20 – 25 | 6 | By similarity | PRO_0000001465 | |||||||
| Chain | 26 – 612 | 587 | Glucoamylase | PRO_0000001466 | |||||||
Regions | |||||||||||
| Domain | 506 – 612 | 107 | CBM20 | ||||||||
Sites | |||||||||||
| Active site | 202 | 1 | Proton acceptor By similarity | ||||||||
| Active site | 205 | 1 | Proton donor By similarity | ||||||||
| Binding site | 146 | 1 | Substrate By similarity | ||||||||
Amino acid modifications | |||||||||||
| Glycosylation | 39 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Disulfide bond | 236 ↔ 239 | By similarity | |||||||||
| Disulfide bond | 248 ↔ 475 | By similarity | |||||||||
| Disulfide bond | 288 ↔ 296 | By similarity | |||||||||
Experimental info | |||||||||||
| Sequence conflict | 172 | 1 | G → S Ref.1 | ||||||||
| Sequence conflict | 172 | 1 | G → S Ref.2 | ||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The glucoamylase cDNA from Aspergillus oryzae: its cloning, nucleotide sequence, and expression in Saccharomyces cerevisiae." Hata Y., Kitamoto K., Gomi K., Kumagai C., Tamura G., Hara S. Agric. Biol. Chem. 55:941-949(1991) [PubMed: 1368680] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "Nucleotide sequence and expression of the glucoamylase-encoding gene (glaA) from Aspergillus oryzae." Hata Y., Tsuchiya K., Kitamoto K., Gomi K., Kumagai C., Tamura G., Hara S. Gene 108:145-150(1991) [PubMed: 1761224] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 42149 / RIB 40. |
| [3] | Ma L., Chen D., Chen X., Wang H. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [4] | "Genome sequencing and analysis of Aspergillus oryzae." Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E. Kikuchi H.Nature 438:1157-1161(2005) [PubMed: 16372010] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 42149 / RIB 40. |
Cross-references
Sequence databases | |
|---|---|
| D01035 mRNA. Translation: BAA00841.1. D10698 Genomic DNA. Translation: BAA01540.1. DQ211971 mRNA. Translation: ABA62323.1. AP007175 Genomic DNA. Translation: BAE66563.1. | |
| PIR | JQ1346. |
| RefSeq | XP_001827696.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1GAI based on UniProtKB P04064. |
| SMR | P36914. Positions 30-495. |
| ModBase | Search... |
Protein family/group databases | |
| CAZy | CBM20. Carbohydrate-Binding Module Family 20. GH15. Glycoside Hydrolase Family 15. |
Genome annotation databases | |
| GeneID | 5999830. |
| GenomeReviews | Gene locus glaA in contig AP007175_GR. |
| KEGG | aor:AO090010000746. |
Enzyme and pathway databases | |
| BRENDA | 3.2.1.3. 2240. |
Family and domain databases | |
| InterPro | IPR012341. 6hp_glycosidase. IPR008291. Glucamylse_SBD. IPR000165. Glyco_hydro_15. IPR011613. Glyco_hydro_15_rel. IPR002044. Glyco_hydro_carb-bd. IPR013783. Ig-like_fold. [Graphical view] |
| Gene3D | G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit. |
| Pfam | PF00686. CBM_20. 1 hit. PF00723. Glyco_hydro_15. 1 hit. [Graphical view] |
| PIRSF | PIRSF001031. Glu-a-glcsd_SBD. 1 hit. |
| PRINTS | PR00736. GLHYDRLASE15. |
| ProDom | PD001568. Glyco_hydro_CBD. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| PROSITE | PS51166. CBM20. 1 hit. PS00820. GLUCOAMYLASE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | AMYG_ASPOR | ||||||||
| Accession | Primary (citable) accession number: P36914 Secondary accession number(s): Q3HLW7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| Glycosyl hydrolases Classification of glycosyl hydrolase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

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