Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glucoamylase

Gene

glaA

Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei146SubstrateBy similarity1
Active sitei202Proton acceptorPROSITE-ProRule annotation1
Active sitei205Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

  • glucan 1,4-alpha-glucosidase activity Source: AspGD
  • starch binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Polysaccharide degradation

Enzyme and pathway databases

BRENDAi3.2.1.3 522

Protein family/group databases

CAZyiCBM20 Carbohydrate-Binding Module Family 20
GH15 Glycoside Hydrolase Family 15

Names & Taxonomyi

Protein namesi
Recommended name:
Glucoamylase (EC:3.2.1.3)
Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
Glucan 1,4-alpha-glucosidase
Gene namesi
Name:glaA
Synonyms:gluB
ORF Names:AO090010000746
OrganismiAspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Taxonomic identifieri510516 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006564 Componenti: Chromosome 8

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19By similarityAdd BLAST19
PropeptideiPRO_000000146520 – 25By similarity6
ChainiPRO_000000146626 – 612GlucoamylaseAdd BLAST587

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi39N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi236 ↔ 239By similarity
Disulfide bondi248 ↔ 475By similarity
Disulfide bondi288 ↔ 296By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi5062.CADAORAP00004691

Structurei

3D structure databases

ProteinModelPortaliP36914
SMRiP36914
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini506 – 612CBM20PROSITE-ProRule annotationAdd BLAST107

Sequence similaritiesi

Belongs to the glycosyl hydrolase 15 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000182646
KOiK01178
OMAiEDSYYNG
OrthoDBiEOG092C1YYH

Family and domain databases

CDDicd05811 CBM20_glucoamylase, 1 hit
Gene3Di1.50.10.10, 1 hit
2.60.40.10, 1 hit
InterProiView protein in InterPro
IPR008928 6-hairpin_glycosidase_sf
IPR012341 6hp_glycosidase-like_sf
IPR013784 Carb-bd-like_fold
IPR034836 CBM20_glucoamylase
IPR002044 CBM_fam20
IPR000165 Glucoamylase
IPR008291 Glucoamylase_SBD
IPR011613 Glyco_hydro_15/PHK
IPR013783 Ig-like_fold
PfamiView protein in Pfam
PF00686 CBM_20, 1 hit
PF00723 Glyco_hydro_15, 1 hit
PIRSFiPIRSF001031 Glu-a-glcsd_SBD, 1 hit
PRINTSiPR00736 GLHYDRLASE15
SMARTiView protein in SMART
SM01065 CBM_2, 1 hit
SUPFAMiSSF48208 SSF48208, 1 hit
SSF49452 SSF49452, 1 hit
PROSITEiView protein in PROSITE
PS51166 CBM20, 1 hit
PS00820 GLUCOAMYLASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36914-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSFSSCLRA LALGSSVLAV QPVLRQATGL DTWLSTEANF SRQAILNNIG
60 70 80 90 100
ADGQSAQGAS PGVVIASPSK SDPDYFYTWT RDSGLVMKTL VDLFRGGDAD
110 120 130 140 150
LLPIIEEFIS SQARIQGISN PSGALSSGGL GEPKFNVDET AFTGAWGRPQ
160 170 180 190 200
RDGPALRATA MISFGEWLVE NGHTSIATDL VWPVVRNDLS YVAQYWSQSG
210 220 230 240 250
FDLWEEVQGT SFFTVAVSHR ALVEGSSFAK TVGSSCPYCD SQAPQVRCYL
260 270 280 290 300
QSFWTGSYIQ ANFGGGRSGK DINTVLGSIH TFDPQATCDD ATFQPCSARA
310 320 330 340 350
LANHKVVTDS FRSIYAINSG RAENQAVAVG RYPEDSYYNG NPWFLTTLAA
360 370 380 390 400
AEQLYDALYQ WDKIGSLAIT DVSLPFFKAL YSSAATGTYA SSTTVYKDIV
410 420 430 440 450
SAVKAYADGY VQIVQTYAAS TGSMAEQYTK TDGSQTSARD LTWSYAALLT
460 470 480 490 500
ANNRRNAVVP APWGETAATS IPSACSTTSA SGTYSSVVIT SWPTISGYPG
510 520 530 540 550
APDSPCQVPT TVSVTFAVKA TTVYGESIKI VGSISQLGSW NPSSATALNA
560 570 580 590 600
DSYTTDNPLW TGTINLPAGQ SFEYKFIRVQ NGAVTWESDP NRKYTVPSTC
610
GVKSAVQSDV WR
Length:612
Mass (Da):65,457
Last modified:May 2, 2006 - v2
Checksum:i7C36E1B0A341CEDA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti172G → S in BAA00841 (PubMed:1368680).Curated1
Sequence conflicti172G → S in BAA01540 (PubMed:1761224).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D01035 mRNA Translation: BAA00841.1
D10698 Genomic DNA Translation: BAA01540.1
DQ211971 mRNA Translation: ABA62323.1
AP007175 Genomic DNA Translation: BAE66563.1
PIRiJQ1346
RefSeqiXP_001827696.1, XM_001827644.2

Genome annotation databases

EnsemblFungiiBAE66563; BAE66563; AO090010000746
GeneIDi5999830
KEGGiaor:AO090010000746

Similar proteinsi

Entry informationi

Entry nameiAMYG_ASPOR
AccessioniPrimary (citable) accession number: P36914
Secondary accession number(s): Q3HLW7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: May 2, 2006
Last modified: March 28, 2018
This is version 120 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health