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P36913

- EBA3_ELIME

UniProt

P36913 - EBA3_ELIME

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Protein

Endo-beta-N-acetylglucosaminidase F3

Gene

endOF3

Organism
Elizabethkingia meningoseptica (Chryseobacterium meningosepticum)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Endohydrolysis of the di-N-acetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins. Hydrolyzes bi- and triantennary glycans. The presence of a core-bound fucose greatly augments endo F3 activity on biantennary and, presumably, triantennary oligosaccharides.

Catalytic activityi

Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)2)Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei167 – 1671Proton donorBy similarity

GO - Molecular functioni

  1. mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH18. Glycoside Hydrolase Family 18.

Names & Taxonomyi

Protein namesi
Recommended name:
Endo-beta-N-acetylglucosaminidase F3 (EC:3.2.1.96)
Alternative name(s):
Di-N-acetylchitobiosyl beta-N-acetylglucosaminidase F3
Endoglycosidase F3
Mannosyl-glycoprotein endo-beta-N-acetyl-glucosaminidase F3
Gene namesi
Name:endOF3
OrganismiElizabethkingia meningoseptica (Chryseobacterium meningosepticum)
Taxonomic identifieri238 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeElizabethkingia

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3939Or 40, or 41Add
BLAST
Chaini40 – 329290Endo-beta-N-acetylglucosaminidase F3PRO_0000011957Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi88 – 881O-linked (Man...)1 Publication

Post-translational modificationi

Carbohydrate at Thr-88 consists of (2-OMe)Man1-4GlcNAcU1-4GlcU1-4Glc1-4(2-OMe)GlcU1-4[(2-OMe)Rham1-2]Man.

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1
329
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi49 – 557Combined sources
Helixi65 – 673Combined sources
Beta strandi74 – 807Combined sources
Helixi82 – 865Combined sources
Helixi96 – 983Combined sources
Beta strandi101 – 1033Combined sources
Helixi104 – 11512Combined sources
Turni116 – 1183Combined sources
Beta strandi120 – 1267Combined sources
Helixi129 – 1324Combined sources
Beta strandi133 – 1353Combined sources
Helixi136 – 1383Combined sources
Beta strandi139 – 1413Combined sources
Helixi142 – 15312Combined sources
Turni154 – 1574Combined sources
Beta strandi161 – 1655Combined sources
Helixi182 – 1854Combined sources
Beta strandi186 – 1894Combined sources
Helixi199 – 20810Combined sources
Turni209 – 2113Combined sources
Beta strandi216 – 2183Combined sources
Beta strandi221 – 2266Combined sources
Helixi233 – 2408Combined sources
Turni241 – 2433Combined sources
Beta strandi245 – 2495Combined sources
Helixi256 – 26813Combined sources
Helixi274 – 2763Combined sources
Beta strandi277 – 2815Combined sources
Turni283 – 2853Combined sources
Helixi289 – 2979Combined sources
Beta strandi307 – 3104Combined sources
Helixi313 – 3153Combined sources
Helixi317 – 32711Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EOKX-ray1.80A40-329[»]
1EOMX-ray2.10A40-329[»]
ProteinModelPortaliP36913.
SMRiP36913. Positions 47-329.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP36913.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 18 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001223. Glyco_hydro18cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00704. Glyco_hydro_18. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36913-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKIFFAQCS ILLLMLGSCS KMTEDMTPES VNKEASVKSA TALAGSNGVC
60 70 80 90 100
IAYYITDGRN PTFKLKDIPD KVDMVILFGL KYWSLQDTTK LPGGTGMMGS
110 120 130 140 150
FKSYKDLDTQ IRSLQSRGIK VLQNIDDDVS WQSSKPGGFA SAAAYGDAIK
160 170 180 190 200
SIVIDKWKLD GISLDIEHSG AKPNPIPTFP GYAATGYNGW YSGSMAATPA
210 220 230 240 250
FLNVISELTK YFGTTAPNNK QLQIASGIDV YAWNKIMENF RNNFNYIQLQ
260 270 280 290 300
SYGANVSRTQ LMMNYATGTN KIPASKMVFG AYAEGGTNQA NDVEVAKWTP
310 320
TQGAKGGMMI YTYNSNVSYA NAVRDAVKN
Length:329
Mass (Da):35,844
Last modified:June 1, 1994 - v1
Checksum:iC12EA4C8920784FF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06332 Genomic DNA. Translation: AAA24924.1.
PIRiB46678.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06332 Genomic DNA. Translation: AAA24924.1 .
PIRi B46678.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1EOK X-ray 1.80 A 40-329 [» ]
1EOM X-ray 2.10 A 40-329 [» ]
ProteinModelPortali P36913.
SMRi P36913. Positions 47-329.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH18. Glycoside Hydrolase Family 18.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P36913.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001223. Glyco_hydro18cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00704. Glyco_hydro_18. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Multiple endoglycosidase F activities expressed by Flavobacterium meningosepticum endoglycosidases F2 and F3. Molecular cloning, primary sequence, and enzyme expression."
    Tarentino A.L., Quinones G., Changchien L.-M., Plummer T.H. Jr.
    J. Biol. Chem. 268:9702-9708(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
  2. "Novel, specific O-glycosylation of secreted Flavobacterium meningosepticum proteins. Asp-Ser and Asp-Thr-Thr consensus sites."
    Plummer T.H. Jr., Tarentino A.L., Hauer C.R.
    J. Biol. Chem. 270:13192-13196(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT THR-88, IDENTIFICATION BY MASS SPECTROMETRY.
  3. "Detailed structural analysis of a novel, specific O-linked glycan from the prokaryote Flavobacterium meningosepticum."
    Reinhold B.B., Hauer C.R., Plummer T.H. Jr., Reinhold V.N.
    J. Biol. Chem. 270:13197-13203(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE OF CARBOHYDRATE, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiEBA3_ELIME
AccessioniPrimary (citable) accession number: P36913
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 26, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3