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P36913

- EBA3_ELIME

UniProt

P36913 - EBA3_ELIME

Protein

Endo-beta-N-acetylglucosaminidase F3

Gene

endOF3

Organism
Elizabethkingia meningoseptica (Chryseobacterium meningosepticum)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Endohydrolysis of the di-N-acetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins. Hydrolyzes bi- and triantennary glycans. The presence of a core-bound fucose greatly augments endo F3 activity on biantennary and, presumably, triantennary oligosaccharides.

    Catalytic activityi

    Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)2)Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei167 – 1671Proton donorBy similarity

    GO - Molecular functioni

    1. mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH18. Glycoside Hydrolase Family 18.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endo-beta-N-acetylglucosaminidase F3 (EC:3.2.1.96)
    Alternative name(s):
    Di-N-acetylchitobiosyl beta-N-acetylglucosaminidase F3
    Endoglycosidase F3
    Mannosyl-glycoprotein endo-beta-N-acetyl-glucosaminidase F3
    Gene namesi
    Name:endOF3
    OrganismiElizabethkingia meningoseptica (Chryseobacterium meningosepticum)
    Taxonomic identifieri238 [NCBI]
    Taxonomic lineageiBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeElizabethkingia

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3939Or 40, or 41Add
    BLAST
    Chaini40 – 329290Endo-beta-N-acetylglucosaminidase F3PRO_0000011957Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi88 – 881O-linked (Man...)1 Publication

    Post-translational modificationi

    Carbohydrate at Thr-88 consists of (2-OMe)Man1-4GlcNAcU1-4GlcU1-4Glc1-4(2-OMe)GlcU1-4[(2-OMe)Rham1-2]Man.

    Keywords - PTMi

    Glycoprotein

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    Secondary structure

    1
    329
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi49 – 557
    Helixi65 – 673
    Beta strandi74 – 807
    Helixi82 – 865
    Helixi96 – 983
    Beta strandi101 – 1033
    Helixi104 – 11512
    Turni116 – 1183
    Beta strandi120 – 1267
    Helixi129 – 1324
    Beta strandi133 – 1353
    Helixi136 – 1383
    Beta strandi139 – 1413
    Helixi142 – 15312
    Turni154 – 1574
    Beta strandi161 – 1655
    Helixi182 – 1854
    Beta strandi186 – 1894
    Helixi199 – 20810
    Turni209 – 2113
    Beta strandi216 – 2183
    Beta strandi221 – 2266
    Helixi233 – 2408
    Turni241 – 2433
    Beta strandi245 – 2495
    Helixi256 – 26813
    Helixi274 – 2763
    Beta strandi277 – 2815
    Turni283 – 2853
    Helixi289 – 2979
    Beta strandi307 – 3104
    Helixi313 – 3153
    Helixi317 – 32711

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1EOKX-ray1.80A40-329[»]
    1EOMX-ray2.10A40-329[»]
    ProteinModelPortaliP36913.
    SMRiP36913. Positions 47-329.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP36913.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 18 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001223. Glyco_hydro18cat.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00704. Glyco_hydro_18. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P36913-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKIFFAQCS ILLLMLGSCS KMTEDMTPES VNKEASVKSA TALAGSNGVC    50
    IAYYITDGRN PTFKLKDIPD KVDMVILFGL KYWSLQDTTK LPGGTGMMGS 100
    FKSYKDLDTQ IRSLQSRGIK VLQNIDDDVS WQSSKPGGFA SAAAYGDAIK 150
    SIVIDKWKLD GISLDIEHSG AKPNPIPTFP GYAATGYNGW YSGSMAATPA 200
    FLNVISELTK YFGTTAPNNK QLQIASGIDV YAWNKIMENF RNNFNYIQLQ 250
    SYGANVSRTQ LMMNYATGTN KIPASKMVFG AYAEGGTNQA NDVEVAKWTP 300
    TQGAKGGMMI YTYNSNVSYA NAVRDAVKN 329
    Length:329
    Mass (Da):35,844
    Last modified:June 1, 1994 - v1
    Checksum:iC12EA4C8920784FF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L06332 Genomic DNA. Translation: AAA24924.1.
    PIRiB46678.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L06332 Genomic DNA. Translation: AAA24924.1 .
    PIRi B46678.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1EOK X-ray 1.80 A 40-329 [» ]
    1EOM X-ray 2.10 A 40-329 [» ]
    ProteinModelPortali P36913.
    SMRi P36913. Positions 47-329.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH18. Glycoside Hydrolase Family 18.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P36913.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001223. Glyco_hydro18cat.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00704. Glyco_hydro_18. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Multiple endoglycosidase F activities expressed by Flavobacterium meningosepticum endoglycosidases F2 and F3. Molecular cloning, primary sequence, and enzyme expression."
      Tarentino A.L., Quinones G., Changchien L.-M., Plummer T.H. Jr.
      J. Biol. Chem. 268:9702-9708(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    2. "Novel, specific O-glycosylation of secreted Flavobacterium meningosepticum proteins. Asp-Ser and Asp-Thr-Thr consensus sites."
      Plummer T.H. Jr., Tarentino A.L., Hauer C.R.
      J. Biol. Chem. 270:13192-13196(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION AT THR-88, IDENTIFICATION BY MASS SPECTROMETRY.
    3. "Detailed structural analysis of a novel, specific O-linked glycan from the prokaryote Flavobacterium meningosepticum."
      Reinhold B.B., Hauer C.R., Plummer T.H. Jr., Reinhold V.N.
      J. Biol. Chem. 270:13197-13203(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE OF CARBOHYDRATE, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiEBA3_ELIME
    AccessioniPrimary (citable) accession number: P36913
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3