P36910 (CHIE_BETVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acidic endochitinase SE2
EC=3.2.1.14

Gene names

Name:

SE2

Organism

Beta vulgaris (Sugar beet)

Taxonomic identifier

161934 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Beta

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein functions as a defense against chitin containing fungal pathogens. This endochitinase also exhibits exochitinase activity, i.e., it is capable of hydrolyzing chito-oligosaccharides, including chitobiose.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Subcellular location	Secreted › extracellular space. Note: Intercellular fluid of leaves.
Tissue specificity	Accumulates in leaves during infection.
Induction	By fungal infection.
Sequence similarities	Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25
	Chain	26 – 293	268	Acidic endochitinase SE2		PRO_0000011914
Sites
	Active site	151	1	Proton donor By similarity
Amino acid modifications
	Disulfide bond	45 ↔ 91		By similarity
	Disulfide bond	75 ↔ 81		By similarity
	Disulfide bond	183 ↔ 212		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P36910 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 0E9019D28FD34123	FASTA	293	30,729
	10 20 30 40 50 60 MAAKIVSVLF LISLLIFASF ESSHGSQIVI YWGQNGDEGS LADTCNSGNY GTVILAFVAT 70 80 90 100 110 120 FGNGQTPALN LAGHCDPATN CNSLSSDIKT CQQAGIKVLL SIGGGAGGYS LSSTDDANTF 130 140 150 160 170 180 ADYLWNTYLG GQSSTRPLGD AVLDGIDFDI ESGDGRFWDD LARALAGHNN GQKTVYLSAA 190 200 210 220 230 240 PQCPLPDASL STAIATGLFD YVWVQFYNNP PCQYDTSADN LLSSWNQWTT VQANQIFLGL 250 260 270 280 290 PASTDAAGSG FIPADALTSQ VLPTIKGSAK YGGVMLWSKA YDSGYSSAIK SSV

References

[1]	"An acidic class III chitinase in sugar beet: induction by Cercospora beticola, characterization, and expression in transgenic tobacco plants." Nielsen K.K., Mikkelsen J.D., Kragh K.M., Bojsen K. Mol. Plant Microbe Interact. 6:495-506(1993) [PubMed: 8400378] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: cv. Monova. Tissue: Leaf.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S66038 mRNA. Translation: AAB28479.1.
3D structure databases
ProteinModelPortal	P36910.
SMR	P36910. Positions 28-293.
ModBase	Search...
Protein family/group databases
CAZy	GH18. Glycoside Hydrolase Family 18.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001223. Glyco_hydro18cat. IPR001579. Glyco_hydro_18_chit_AS. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00704. Glyco_hydro_18. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS01095. CHITINASE_18. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHIE_BETVU

Accession

Primary (citable) accession number: P36910

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 31, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program