P36909 (CHIT_STRLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitinase C
EC=3.2.1.14

Gene names

Name:

chiC

Organism

Streptomyces lividans

Taxonomic identifier

1916 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	619 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Induction	By chitin.
Sequence similarities	Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily. Contains 1 CBM2 (carbohydrate binding type-2) domain. Contains 1 fibronectin type-III domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Polysaccharide degradation
Domain	Signal
Ligand	Chitin-binding
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological_process	chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activity Inferred from electronic annotation. Source: EC polysaccharide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Potential
	Chain	31 – 619	589	Chitinase C		PRO_0000011911
Regions
	Domain	31 – 134	104	CBM2
	Domain	142 – 224	83	Fibronectin type-III
	Region	240 – 619	380	Catalytic
Sites
	Active site	382	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P36909 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: A23CEE5B3C5D6F21	FASTA	619	65,200
	10 20 30 40 50 60 MRFRHKAAAL AATLALPLAG LVGLASPAQA ATSATATFAK TSDWGTGFGG SWTVKNTGTT 70 80 90 100 110 120 SLSSWTVEWD FPTGTKVTSA WDATVTNSGD HWTAKNVGWN GTLAPGASVS FGFNGSGPGS 130 140 150 160 170 180 PSNCKLNGGS CDGTSVPGDA APSAPGTPTA SNITDTSVKL SWSAATDDKG VKNYDVLRDG 190 200 210 220 230 240 AKVATVTGTT YTDNGLTKGT AYSYSVKARD TADQTGPASG AVKVTTTGGG DGGNPGTGAE 250 260 270 280 290 300 VKMGYFTNWG VYGRNYHVKN LVTSGSADKI THINYAFGNV QGGKCTIGDS YADYDKAYTA 310 320 330 340 350 360 DQSVDGVADT WDQPLRGNFN QLRKLKAKYP NIKILYSFGG WTWSGGFPDA VKNPAAFAKS 370 380 390 400 410 420 CHDLVEDPRW ADVFDGIDLD WEYPNACGLS CDETSAPNAF SSMMKAMRAE FGQDYLITAA 430 440 450 460 470 480 VTADGSDGGK IDAADYGEAS KYIDWYNVMT YDFFGAWAKN GPTAPHSPLT AYDGIPQQGF 490 500 510 520 530 540 NTADAMAKFK SKGVPADKLL IGIGFYGRGW TGVTQSAPGG TATGPATGTY EAGIEDYKVL 550 560 570 580 590 600 KNSCPATGTI AGTAYAHCGS NWWSYDTPAT IKSKMDWAEQ QGLGGAFFWE FSGDTANGDW 610 WRHRQRPQVT PAVRTTRRH

References

[1]	"Multiple domain structure in a chitinase gene (chiC) of Streptomyces lividans." Fujii T., Miyashita K. J. Gen. Microbiol. 139:677-686(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	D12647 Genomic DNA. Translation: BAA02168.1.
3D structure databases
ProteinModelPortal	P36909.
ModBase	Search...
Protein family/group databases
CAZy	CBM2. Carbohydrate-Binding Module Family 2. GH18. Glycoside Hydrolase Family 18.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.60.40.10. 1 hit. 2.60.40.290. 1 hit. 3.20.20.80. 2 hits.
InterPro	IPR008965. Carb-bd_dom. IPR012291. CBD_carb-bd_dom. IPR018366. CBM2_CS. IPR001919. Cellulose-bd_dom_fam2_bac. IPR011583. Chitinase_II. IPR003961. Fibronectin_type3. IPR001223. Glyco_hydro18cat. IPR001579. Glyco_hydro_18_chit_AS. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. [Graphical view]
Pfam	PF00553. CBM_2. 1 hit. PF00041. fn3. 1 hit. PF00704. Glyco_hydro_18. 1 hit. [Graphical view]
SMART	SM00637. CBD_II. 1 hit. SM00060. FN3. 1 hit. SM00636. Glyco_18. 1 hit. [Graphical view]
SUPFAM	SSF49384. Cellul_bind. 1 hit. SSF49265. FN_III-like. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS51173. CBM2. 1 hit. PS00561. CBM2_A. 1 hit. PS01095. CHITINASE_18. 1 hit. PS00018. EF_HAND_1. 1 hit. Uncertain. PS50853. FN3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHIT_STRLI

Accession

Primary (citable) accession number: P36909

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program