Reviewed, UniProtKB/Swiss-Prot P36908 (CHIA_CICAR)

Names and origin

Protein names	Recommended name:     Acidic endochitinase     EC=3.2.1.14
Organism	Cicer arietinum (Chickpea) (Garbanzo)
Taxonomic identifier	3827 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Cicereae › Cicer

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This protein functions as a defense against chitin containing fungal pathogens.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 293	271	Acidic endochitinase		PRO_0000011915
Sites
	Active site	150	1	Proton donor By similarity
Amino acid modifications
	Disulfide bond	43 ↔ 90		By similarity
	Disulfide bond	73 ↔ 80		By similarity
	Disulfide bond	179 ↔ 208		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P36908-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: F7C7DFC17A5720F4	FASTA	293	31,230
	10 20 30 40 50 60 MEKCFNIIPS LLLISLLIKS SNAAGIAVYW GQNGNEGSLQ DACNTNNYQF VNIAFLSTFG 70 80 90 100 110 120 NGQNPQINLA GHCDPSTNGC TKFSPEIQAC QAKGIKVLLS LGGGAGSYSL NSAEEATTLA 130 140 150 160 170 180 NYLWNNFLGG TSTSRPLGDA VLDGIDFDIE SGGQHYDELA KALNGFSQQK VYLSAAPQCP 190 200 210 220 230 240 YPDAHLDSAI QTGLFDYVWV QFYNNPQCQY SNGNINNLVN AWNQWTSSQA KQVFLGVPAS 250 260 270 280 290 DAAAPSGGLI PADVLTSQVL PAIKTSPKYG GVMIWDRFND AQSGYSNAIK GSV

References

[1]	"Cloning of a class III acidic chitinase from chickpea." Vogelsang R., Barz W. Plant Physiol. 103:297-298(1993) [PubMed: 8208854] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. ILC 3279.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X70660 mRNA. Translation: CAA49998.1.
PIR	S31763.
3D structure databases
HSSP	HSSP built from PDB template 2HVM based on UniProtKB P23472.
ModBase	Search...
Protein family/group databases
CAZy	GH18. Glycoside Hydrolase Family 18.
Enzyme and pathway databases
BRENDA	3.2.1.14. 475.
Family and domain databases
InterPro	IPR001223. Glyco_hydro18cat. IPR001579. Glyco_hydro_18_chit_AS. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00704. Glyco_hydro_18. 1 hit. [Graphical view]
PROSITE	PS01095. CHITINASE_18. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHIA_CICAR

Accession

Primary (citable) accession number: P36908

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	September 22, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)