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P36905

- APU_THESA

UniProt

P36905 - APU_THESA

Protein

Amylopullulanase

Gene

apu

Organism
Thermoanaerobacterium saccharolyticum
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 2 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.
    Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the alpha- and beta-limit dextrins of amylopectin and glycogen.

    Cofactori

    Binds 1 calcium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi401 – 4011CalciumBy similarity
    Metal bindingi403 – 4031Calcium; via carbonyl oxygenBy similarity
    Metal bindingi406 – 4061CalciumBy similarity
    Metal bindingi407 – 4071CalciumBy similarity
    Metal bindingi452 – 4521Calcium; via carbonyl oxygenBy similarity
    Metal bindingi454 – 4541CalciumBy similarity
    Binding sitei527 – 5271SubstrateBy similarity
    Binding sitei627 – 6271SubstrateBy similarity
    Active sitei629 – 6291NucleophileBy similarity
    Active sitei658 – 6581Proton donorBy similarity
    Sitei735 – 7351Transition state stabilizerBy similarity
    Binding sitei798 – 7981SubstrateBy similarity

    GO - Molecular functioni

    1. alpha-amylase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW
    3. pullulanase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism

    Keywords - Ligandi

    Calcium, Metal-binding

    Protein family/group databases

    CAZyiCBM20. Carbohydrate-Binding Module Family 20.
    CBM34. Carbohydrate-Binding Module Family 34.
    GH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Amylopullulanase
    Alternative name(s):
    Alpha-amylase/pullulanase
    Including the following 2 domains:
    Alpha-amylase (EC:3.2.1.1)
    Alternative name(s):
    1,4-alpha-D-glucan glucanohydrolase
    Pullulanase (EC:3.2.1.41)
    Alternative name(s):
    1,4-alpha-D-glucan glucanohydrolase
    Alpha-dextrin endo-1,6-alpha-glucosidase
    Gene namesi
    Name:apu
    OrganismiThermoanaerobacterium saccharolyticum
    Taxonomic identifieri28896 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacterales Family III. Incertae SedisThermoanaerobacterium

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3535Sequence AnalysisAdd
    BLAST
    Chaini36 – 12791244AmylopullulanasePRO_0000001324Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP36905.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini930 – 102293Fibronectin type-III 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini1158 – 125295Fibronectin type-III 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 13 family.Curated
    Contains 2 fibronectin type-III domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Family and domain databases

    Gene3Di2.60.40.10. 3 hits.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR006048. A-amylase_b_C.
    IPR003961. Fibronectin_type3.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004185. Glyco_hydro_13_lg-like_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF02903. Alpha-amylase_N. 1 hit.
    PF00041. fn3. 2 hits.
    [Graphical view]
    SMARTiSM00642. Aamy. 1 hit.
    SM00632. Aamy_C. 1 hit.
    SM00060. FN3. 2 hits.
    [Graphical view]
    SUPFAMiSSF49265. SSF49265. 2 hits.
    SSF51445. SSF51445. 2 hits.
    SSF81296. SSF81296. 1 hit.
    PROSITEiPS50853. FN3. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P36905-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYKKLFTKKF ISFVMSLLLV LTAAFSSMPF HNVYAADNAS VVANIVGDFQ     50
    DQLGDSNWNI DSNITIMQYV GNGLYEFTTP TQLKAGSYQY KVALNHSWNG 100
    GGVPSQGNLT LNLTNDSYVT FWFDYNTQSV TDSTKYTPIS NDKLPRLVGT 150
    IQSAIGAGKD WDPGTSTAIM IDDNFDNVYS YTAHIPKGDY QYKVTLGNTW 200
    AENYGANGVQ DGSNIQLSVA NDADITFFYD ANTHNIWTNY SPTLTGLDNN 250
    IYYDDLKHDT HDPFFRNPFG AIKVGQTVTL RIQAKNHDLE SARISYWDDI 300
    NKTRTELPMT RIGESPDGNY EYWEIKLSFD HPTRIWYYFI LKDGTKTAYY 350
    GDNDDQLGGL GKATDTVNKD FELTVYDKNF DTNDWMKGAV MYQIFPDRFY 400
    NGDTSNDHAK TLSRGNDPIE FHNDWNDLPD NPNNAGTPGY TGDGIWSNDF 450
    FGGDLKGIDD KLDYLKGLGV SVIYLNPIFE SPSNHKYDTA DYTKIDEMFG 500
    TTQDFEKLMS DAHAKGINII LDGVFNHTSD DSIYFNRYGK YPDLGAYQDW 550
    KDGNQSLSPY GDWYTINSDG TYECWWGYDS LPVIKSLNGS EYNVTSWANF 600
    IINDKNAISK YWLNPDENLN DGADGWRLDV ENEVAHDFWT HFRDAINTVK 650
    PEAPMIAENW GDASLDLLGD SFNSVMNYQF RNDIIDFLIG QSFDDGNGQH 700
    NPIDAAKLDQ RLMSIYERYP LPAFYSTMNL LGSHDTMSIL TVFGYNSADP 750
    NENSDAAKRL AEQKLKLATI LQMGYPGMAD IYYGDEAGVS GGKDPDDRRT 800
    FPWGNEDTAL QDFFKNVSSI RNNNQVLKTG DLETLYAQND VYAIGRRIIN 850
    GKDAFGNSYP DSAAIVAINR SNSDQQITID TTKFLRDGVA FKDLINGDKS 900
    YTINGGQITI NIPAMSGVML ISDDGQDLTA PQVPSNVVAT SGNGKVDLSW 950
    SQSDGATGYN IYRSSVEGGL YEKIASNVTG TTFEDTNVTN GLKYVYAISA 1000
    VDELGNESEM SIDTVAYPAY PIGWVGNLTQ VVDNHVISVS NPTEDIYAEV 1050
    WADGLTNSTG QGPNMIAQLG YKYVGGTVND SVYGSVYNSV YGVDDSDFTW 1100
    VNAQYVGDIG NNDQYKASLH LINRSMGYLM RFSDNQGQSW TTTDTLSFYV 1150
    VPSDDLIKPT APILNQPGVE SSRVSLTWSP STDNVGIYNY EIYRSDGGTF 1200
    NKIATVSNEV YNYVDTSVIN GTTYSYKVVA ADPSFNRTES NVVTIKPDVV 1250
    PIKVTFNVTV PDYTPNSVNL AGTFPNATW 1279
    Length:1,279
    Mass (Da):142,431
    Last modified:February 1, 1995 - v2
    Checksum:i095CCBCA391624DD
    GO

    Sequence cautioni

    The sequence AAA19800.1 differs from that shown. Reason: Contaminating sequence. Vector contamination at the C-terminus, a segment of 9 residues, which seems to originate from a puc-type vector.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L07762 Unassigned DNA. Translation: AAA19800.1. Sequence problems.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L07762 Unassigned DNA. Translation: AAA19800.1 . Sequence problems.

    3D structure databases

    ProteinModelPortali P36905.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM20. Carbohydrate-Binding Module Family 20.
    CBM34. Carbohydrate-Binding Module Family 34.
    GH13. Glycoside Hydrolase Family 13.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.40.10. 3 hits.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR006048. A-amylase_b_C.
    IPR003961. Fibronectin_type3.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004185. Glyco_hydro_13_lg-like_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF02903. Alpha-amylase_N. 1 hit.
    PF00041. fn3. 2 hits.
    [Graphical view ]
    SMARTi SM00642. Aamy. 1 hit.
    SM00632. Aamy_C. 1 hit.
    SM00060. FN3. 2 hits.
    [Graphical view ]
    SUPFAMi SSF49265. SSF49265. 2 hits.
    SSF51445. SSF51445. 2 hits.
    SSF81296. SSF81296. 1 hit.
    PROSITEi PS50853. FN3. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of the Thermoanaerobacterium saccharolyticum B6A-RI apu gene and purification and characterization of the amylopullulanase from Escherichia coli."
      Ramesh M.V., Podkovyrov S.M., Lowe S.E., Zeikus J.G.
      Appl. Environ. Microbiol. 60:94-101(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 49915 / DSM 7060 / B6A-RI.
    2. Robison K.
      Unpublished observations (NOV-1994)
      Cited for: IDENTIFICATION OF PROBABLE VECTOR CONTAMINATION.

    Entry informationi

    Entry nameiAPU_THESA
    AccessioniPrimary (citable) accession number: P36905
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 95 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3