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Protein

Adenosylhomocysteinase

Gene
N/A
Organism
Leishmania donovani
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.

Catalytic activityi

S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine.

Cofactori

NAD+Note: Binds 1 NAD+ per subunit.

Pathwayi: L-homocysteine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-homocysteine from S-adenosyl-L-homocysteine.
Proteins known to be involved in this subpathway in this organism are:
  1. Adenosylhomocysteinase, Adenosylhomocysteinase
This subpathway is part of the pathway L-homocysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-homocysteine from S-adenosyl-L-homocysteine, the pathway L-homocysteine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei54SubstrateBy similarity1
Binding sitei130SubstrateBy similarity1
Binding sitei155SubstrateBy similarity1
Binding sitei185SubstrateBy similarity1
Binding sitei189SubstrateBy similarity1
Binding sitei190NADBy similarity1
Binding sitei242NADBy similarity1
Binding sitei277NADBy similarity1
Binding sitei345NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi156 – 158NADBy similarity3
Nucleotide bindingi219 – 224NADBy similarity6
Nucleotide bindingi298 – 300NADBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processOne-carbon metabolism
LigandNAD

Enzyme and pathway databases

UniPathwayiUPA00314; UER00076.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosylhomocysteinase (EC:3.3.1.1)
Short name:
AdoHcyase
Alternative name(s):
S-adenosyl-L-homocysteine hydrolase
OrganismiLeishmania donovani
Taxonomic identifieri5661 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeLeishmania

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001169151 – 437AdenosylhomocysteinaseAdd BLAST437

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP36889.
SMRiP36889.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the adenosylhomocysteinase family.Curated

Phylogenomic databases

eggNOGiKOG1370. Eukaryota.
COG0499. LUCA.

Family and domain databases

CDDicd00401. SAHH. 1 hit.
HAMAPiMF_00563. AdoHcyase. 1 hit.
InterProiView protein in InterPro
IPR034373. Adenosylhomocysteinase.
IPR000043. Adenosylhomocysteinase-like.
IPR015878. Ado_hCys_hydrolase_NAD-bd.
IPR036291. NAD(P)-bd_dom_sf.
IPR020082. S-Ado-L-homoCys_hydrolase_CS.
PANTHERiPTHR23420. PTHR23420. 1 hit.
PfamiView protein in Pfam
PF05221. AdoHcyase. 2 hits.
PF00670. AdoHcyase_NAD. 1 hit.
PIRSFiPIRSF001109. Ad_hcy_hydrolase. 1 hit.
SMARTiView protein in SMART
SM00996. AdoHcyase. 1 hit.
SM00997. AdoHcyase_NAD. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00936. ahcY. 1 hit.
PROSITEiView protein in PROSITE
PS00738. ADOHCYASE_1. 1 hit.
PS00739. ADOHCYASE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P36889-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADYKVKDIS LAEWGRKAIE LAENEMPGLM ELRREYGPSQ PLKGAKIAGC
60 70 80 90 100
LHMTVQTAVL IETLKALGAD VRWSSCNIFS TQDNAAAAIA KAGVPVFAWK
110 120 130 140 150
GETDEEYEWC IAQTVKGFSG DGLPNMILDD GGDLTNLVID HHPELVPKIF
160 170 180 190 200
GISEETTTGV KNLYKRLSKG NLPMCAINVN DSVTKSKFDN LYGCRESLVD
210 220 230 240 250
GMKRATDVMI AGKTCCVCGY GDVGKGCAAA LRAFGARVVV TEVDPINALQ
260 270 280 290 300
ASMEGYQVAL VEDVMADAHI FVTTTGNDDI ITSEHFPHMR DDAIVCNIGH
310 320 330 340 350
FDTEIQVGWL EANAKEHVEI KPQVDRYTME NGRHIILLAK GRLVNLGCAS
360 370 380 390 400
GHPSFVMSNS FTNQVLAQIE LWSNRDNGKY PRGDKAGVFF LPKALDEKVA
410 420 430
ALHLAHVGAK LTKLTPKQAE YINCPVNGPF KPDHYRY
Length:437
Mass (Da):47,792
Last modified:October 1, 1996 - v2
Checksum:iD687C918DE2B0637
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76556 Genomic DNA. Translation: AAA29265.1.
PIRiA45569.

Similar proteinsi

Entry informationi

Entry nameiSAHH_LEIDO
AccessioniPrimary (citable) accession number: P36889
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: October 1, 1996
Last modified: October 25, 2017
This is version 94 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families