Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein phosphatase PP2A 55 kDa regulatory subunit

Gene

tws

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Could perform a substrate recognition function or could be responsible for targeting the enzyme complex to the appropriate subcellular compartment.

GO - Molecular functioni

  • protein phosphatase type 2A regulator activity Source: FlyBase
  • protein serine/threonine phosphatase activity Source: UniProtKB

GO - Biological processi

  • centriole replication Source: FlyBase
  • centrosome organization Source: FlyBase
  • embryonic development via the syncytial blastoderm Source: FlyBase
  • imaginal disc pattern formation Source: UniProtKB
  • maintenance of neuroblast polarity Source: FlyBase
  • metaphase/anaphase transition of mitotic cell cycle Source: FlyBase
  • mitotic cell cycle Source: FlyBase
  • negative regulation of neuroblast proliferation Source: FlyBase
  • protein dephosphorylation Source: FlyBase
  • protein stabilization Source: FlyBase
  • regulation of mitotic metaphase/anaphase transition Source: UniProtKB
  • sensory organ development Source: FlyBase
  • Wnt signaling pathway Source: FlyBase
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-DME-2995383. Initiation of Nuclear Envelope Reformation.
R-DME-69231. Cyclin D associated events in G1.
R-DME-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
SignaLinkiP36872.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase PP2A 55 kDa regulatory subunit
Short name:
PR55
Alternative name(s):
Protein phosphatase PP2A regulatory subunit B
Protein twins
Gene namesi
Name:tws
Synonyms:aar, Pp2A-85F
ORF Names:CG6235
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0004889. tws.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: FlyBase
  • cytosol Source: FlyBase
  • microtubule associated complex Source: FlyBase
  • nucleus Source: FlyBase
  • protein phosphatase type 2A complex Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 499499Protein phosphatase PP2A 55 kDa regulatory subunitPRO_0000071437Add
BLAST

Proteomic databases

PaxDbiP36872.
PRIDEiP36872.

Expressioni

Gene expression databases

BgeeiFBgn0004889.
ExpressionAtlasiP36872. differential.
GenevisibleiP36872. DM.

Interactioni

Subunit structurei

PP2A exists in several trimeric forms, all of which consist of a core composed of a catalytic subunit associated with a 65 kDa regulatory subunit (PR65) (subunit A). The core complex associates with a third, variable subunit (subunit B), which confers distinct properties to the holoenzyme.

Protein-protein interaction databases

BioGridi71024. 15 interactions.
DIPiDIP-19897N.
IntActiP36872. 31 interactions.
MINTiMINT-896238.
STRINGi7227.FBpp0081665.

Structurei

3D structure databases

ProteinModelPortaliP36872.
SMRiP36872. Positions 62-498.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati79 – 11840WD 1Add
BLAST
Repeati144 – 18542WD 2Add
BLAST
Repeati228 – 26639WD 3Add
BLAST
Repeati277 – 31741WD 4Add
BLAST
Repeati336 – 37439WD 5Add
BLAST
Repeati391 – 43242WD 6Add
BLAST
Repeati467 – 49832WD 7Add
BLAST

Sequence similaritiesi

Contains 7 WD repeats.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1354. Eukaryota.
COG5170. LUCA.
GeneTreeiENSGT00390000006311.
InParanoidiP36872.
KOiK04354.
OMAiDSHAKIF.
OrthoDBiEOG091G09BB.
PhylomeDBiP36872.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR000009. PP2A_PR55.
IPR018067. PP2A_PR55_CS.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR11871. PTHR11871. 1 hit.
PIRSFiPIRSF037309. PP2A_PR55. 1 hit.
PRINTSiPR00600. PP2APR55.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 3 hits.
PROSITEiPS01024. PR55_1. 1 hit.
PS01025. PR55_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P36872-1) [UniParc]FASTAAdd to basket
Also known as: F

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRWGRQSPV LEPPDPQMQT TPPPPTLPPR TFMRQSSITK IGNMLNTAIN
60 70 80 90 100
INGAKKPASN GEASWCFSQI KGALDDDVTD ADIISCVEFN HDGELLATGD
110 120 130 140 150
KGGRVVIFQR DPASKAANPR RGEYNVYSTF QSHEPEFDYL KSLEIEEKIN
160 170 180 190 200
KIRWLQQKNP VHFLLSTNDK TVKLWKVSER DKSFGGYNTK EENGLIRDPQ
210 220 230 240 250
NVTALRVPSV KQIPLLVEAS PRRTFANAHT YHINSISVNS DQETFLSADD
260 270 280 290 300
LRINLWHLEV VNQSYNIVDI KPTNMEELTE VITAAEFHPT ECNVFVYSSS
310 320 330 340 350
KGTIRLCDMR SAALCDRHSK QFEEPENPTN RSFFSEIISS ISDVKLSNSG
360 370 380 390 400
RYMISRDYLS IKVWDLHMET KPIETYPVHE YLRAKLCSLY ENDCIFDKFE
410 420 430 440 450
CCWNGKDSSI MTGSYNNFFR VFDRNSKKDV TLEASRDIIK PKTVLKPRKV
460 470 480 490
CTGGKRKKDE ISVDCLDFNK KILHTAWHPE ENIIAVAATN NLFIFQDKF
Length:499
Mass (Da):56,967
Last modified:June 1, 1994 - v1
Checksum:iD871A7E3058B7286
GO
Isoform B (identifier: P36872-2) [UniParc]FASTAAdd to basket
Also known as: C, D, E, G, H

The sequence of this isoform differs from the canonical sequence as follows:
     1-56: Missing.
     57-59: PAS → MAG

Show »
Length:443
Mass (Da):50,789
Checksum:i83E944093F5BC640
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti211 – 2111K → M in AAA99871 (PubMed:8382567).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5656Missing in isoform B. 2 PublicationsVSP_005105Add
BLAST
Alternative sequencei57 – 593PAS → MAG in isoform B. 2 PublicationsVSP_005106

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13004 mRNA. Translation: BAA02367.1.
L07581 mRNA. Translation: AAA99870.1.
L07583 mRNA. Translation: AAA99871.1.
L07585 Genomic DNA. No translation available.
L07586, L12544 Genomic DNA. Translation: AAB00371.1.
L07586, L12544 Genomic DNA. Translation: AAB00372.1.
AE014297 Genomic DNA. Translation: AAF54498.1.
AE014297 Genomic DNA. Translation: AAF54499.3.
AE014297 Genomic DNA. Translation: AAN13455.1.
AE014297 Genomic DNA. Translation: AAN13456.1.
AE014297 Genomic DNA. Translation: AAN13457.1.
AE014297 Genomic DNA. Translation: AAN13458.1.
AE014297 Genomic DNA. Translation: AAN13459.1.
AE014297 Genomic DNA. Translation: AAN13460.1.
AY061152 mRNA. Translation: AAL28700.1.
PIRiA45778.
RefSeqiNP_001287269.1. NM_001300340.1. [P36872-1]
NP_476880.1. NM_057532.4. [P36872-1]
NP_476881.1. NM_057533.3. [P36872-2]
NP_599111.1. NM_134284.3. [P36872-2]
NP_599112.1. NM_134285.2. [P36872-2]
NP_599113.1. NM_134286.3. [P36872-2]
NP_731451.1. NM_169329.2. [P36872-1]
NP_731452.1. NM_169330.2. [P36872-2]
NP_731453.1. NM_169331.2. [P36872-2]
UniGeneiDm.3826.

Genome annotation databases

EnsemblMetazoaiFBtr0082186; FBpp0081664; FBgn0004889. [P36872-1]
FBtr0082187; FBpp0081665; FBgn0004889. [P36872-1]
FBtr0345177; FBpp0311386; FBgn0004889. [P36872-1]
GeneIDi47877.
KEGGidme:Dmel_CG6235.
UCSCiCG6235-RC. d. melanogaster.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13004 mRNA. Translation: BAA02367.1.
L07581 mRNA. Translation: AAA99870.1.
L07583 mRNA. Translation: AAA99871.1.
L07585 Genomic DNA. No translation available.
L07586, L12544 Genomic DNA. Translation: AAB00371.1.
L07586, L12544 Genomic DNA. Translation: AAB00372.1.
AE014297 Genomic DNA. Translation: AAF54498.1.
AE014297 Genomic DNA. Translation: AAF54499.3.
AE014297 Genomic DNA. Translation: AAN13455.1.
AE014297 Genomic DNA. Translation: AAN13456.1.
AE014297 Genomic DNA. Translation: AAN13457.1.
AE014297 Genomic DNA. Translation: AAN13458.1.
AE014297 Genomic DNA. Translation: AAN13459.1.
AE014297 Genomic DNA. Translation: AAN13460.1.
AY061152 mRNA. Translation: AAL28700.1.
PIRiA45778.
RefSeqiNP_001287269.1. NM_001300340.1. [P36872-1]
NP_476880.1. NM_057532.4. [P36872-1]
NP_476881.1. NM_057533.3. [P36872-2]
NP_599111.1. NM_134284.3. [P36872-2]
NP_599112.1. NM_134285.2. [P36872-2]
NP_599113.1. NM_134286.3. [P36872-2]
NP_731451.1. NM_169329.2. [P36872-1]
NP_731452.1. NM_169330.2. [P36872-2]
NP_731453.1. NM_169331.2. [P36872-2]
UniGeneiDm.3826.

3D structure databases

ProteinModelPortaliP36872.
SMRiP36872. Positions 62-498.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi71024. 15 interactions.
DIPiDIP-19897N.
IntActiP36872. 31 interactions.
MINTiMINT-896238.
STRINGi7227.FBpp0081665.

Proteomic databases

PaxDbiP36872.
PRIDEiP36872.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0082186; FBpp0081664; FBgn0004889. [P36872-1]
FBtr0082187; FBpp0081665; FBgn0004889. [P36872-1]
FBtr0345177; FBpp0311386; FBgn0004889. [P36872-1]
GeneIDi47877.
KEGGidme:Dmel_CG6235.
UCSCiCG6235-RC. d. melanogaster.

Organism-specific databases

CTDi47877.
FlyBaseiFBgn0004889. tws.

Phylogenomic databases

eggNOGiKOG1354. Eukaryota.
COG5170. LUCA.
GeneTreeiENSGT00390000006311.
InParanoidiP36872.
KOiK04354.
OMAiDSHAKIF.
OrthoDBiEOG091G09BB.
PhylomeDBiP36872.

Enzyme and pathway databases

ReactomeiR-DME-2995383. Initiation of Nuclear Envelope Reformation.
R-DME-69231. Cyclin D associated events in G1.
R-DME-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
SignaLinkiP36872.

Miscellaneous databases

ChiTaRSitws. fly.
GenomeRNAii47877.
PROiP36872.

Gene expression databases

BgeeiFBgn0004889.
ExpressionAtlasiP36872. differential.
GenevisibleiP36872. DM.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR000009. PP2A_PR55.
IPR018067. PP2A_PR55_CS.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR11871. PTHR11871. 1 hit.
PIRSFiPIRSF037309. PP2A_PR55. 1 hit.
PRINTSiPR00600. PP2APR55.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 3 hits.
PROSITEiPS01024. PR55_1. 1 hit.
PS01025. PR55_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei2ABA_DROME
AccessioniPrimary (citable) accession number: P36872
Secondary accession number(s): A4V2M9
, A4V2N0, Q9VH21, Q9VH22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 7, 2016
This is version 160 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Caution

It is uncertain whether Met-1, Met-18, Met-33 or Met-44 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.