Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein phosphatase PP2A 55 kDa regulatory subunit

Gene

tws

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Could perform a substrate recognition function or could be responsible for targeting the enzyme complex to the appropriate subcellular compartment.

Caution

It is uncertain whether Met-1, Met-18, Met-33 or Met-44 is the initiator.Curated

GO - Molecular functioni

  • protein phosphatase regulator activity Source: FlyBase
  • protein serine/threonine phosphatase activity Source: UniProtKB

GO - Biological processi

  • centriole replication Source: FlyBase
  • centrosome cycle Source: FlyBase
  • circadian rhythm Source: Reactome
  • embryonic development via the syncytial blastoderm Source: FlyBase
  • imaginal disc pattern formation Source: UniProtKB
  • maintenance of neuroblast polarity Source: FlyBase
  • metaphase/anaphase transition of mitotic cell cycle Source: FlyBase
  • mitotic cell cycle Source: FlyBase
  • negative regulation of neuroblast proliferation Source: FlyBase
  • peptidyl-serine dephosphorylation Source: GO_Central
  • positive regulation of canonical Wnt signaling pathway Source: FlyBase
  • protein dephosphorylation Source: FlyBase
  • protein stabilization Source: FlyBase
  • regulation of mitotic metaphase/anaphase transition Source: UniProtKB
  • sensory organ development Source: FlyBase

Enzyme and pathway databases

ReactomeiR-DME-209155 Phosphorylation of AXN and APC
R-DME-209190 Phosphorylation of CI
R-DME-209214 Phosphorylation of SMO
R-DME-209360 Ubiquitination and proteolysis of phosphorylated CI
R-DME-209396 Phosphorylation of ARM
R-DME-209413 Assembly of the 'destruction complex'
R-DME-209440 Recruitment of the 'destruction complex' to the receptor complex, the degradation of AXN and release of ARM
R-DME-209461 Ubiquitination and degradation of phosphorylated ARM
R-DME-2995383 Initiation of Nuclear Envelope Reformation
R-DME-432553 Phosphorylation of PER and TIM
R-DME-432620 Dephosphorylation of PER
R-DME-69231 Cyclin D associated events in G1
R-DME-69273 Cyclin A/B1/B2 associated events during G2/M transition
R-DME-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)
SignaLinkiP36872

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase PP2A 55 kDa regulatory subunit
Short name:
PR55
Alternative name(s):
Protein phosphatase PP2A regulatory subunit B
Protein twins
Gene namesi
Name:tws
Synonyms:aar, Pp2A-85F
ORF Names:CG6235
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0004889 tws

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000714371 – 499Protein phosphatase PP2A 55 kDa regulatory subunitAdd BLAST499

Proteomic databases

PaxDbiP36872
PRIDEiP36872

Expressioni

Gene expression databases

BgeeiFBgn0004889
ExpressionAtlasiP36872 baseline and differential
GenevisibleiP36872 DM

Interactioni

Subunit structurei

PP2A exists in several trimeric forms, all of which consist of a core composed of a catalytic subunit associated with a 65 kDa regulatory subunit (PR65) (subunit A). The core complex associates with a third, variable subunit (subunit B), which confers distinct properties to the holoenzyme.

Protein-protein interaction databases

BioGridi71024, 27 interactors
DIPiDIP-19897N
IntActiP36872, 31 interactors
STRINGi7227.FBpp0081665

Structurei

3D structure databases

ProteinModelPortaliP36872
SMRiP36872
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati79 – 118WD 1Add BLAST40
Repeati144 – 185WD 2Add BLAST42
Repeati228 – 266WD 3Add BLAST39
Repeati277 – 317WD 4Add BLAST41
Repeati336 – 374WD 5Add BLAST39
Repeati391 – 432WD 6Add BLAST42
Repeati467 – 498WD 7Add BLAST32

Sequence similaritiesi

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1354 Eukaryota
COG5170 LUCA
GeneTreeiENSGT00390000006311
InParanoidiP36872
KOiK04354
OMAiYSNAHAY
OrthoDBiEOG091G09BB
PhylomeDBiP36872

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR000009 PP2A_PR55
IPR018067 PP2A_PR55_CS
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR11871 PTHR11871, 1 hit
PIRSFiPIRSF037309 PP2A_PR55, 1 hit
PRINTSiPR00600 PP2APR55
SMARTiView protein in SMART
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 3 hits
PROSITEiView protein in PROSITE
PS01024 PR55_1, 1 hit
PS01025 PR55_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P36872-1) [UniParc]FASTAAdd to basket
Also known as: F

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRWGRQSPV LEPPDPQMQT TPPPPTLPPR TFMRQSSITK IGNMLNTAIN
60 70 80 90 100
INGAKKPASN GEASWCFSQI KGALDDDVTD ADIISCVEFN HDGELLATGD
110 120 130 140 150
KGGRVVIFQR DPASKAANPR RGEYNVYSTF QSHEPEFDYL KSLEIEEKIN
160 170 180 190 200
KIRWLQQKNP VHFLLSTNDK TVKLWKVSER DKSFGGYNTK EENGLIRDPQ
210 220 230 240 250
NVTALRVPSV KQIPLLVEAS PRRTFANAHT YHINSISVNS DQETFLSADD
260 270 280 290 300
LRINLWHLEV VNQSYNIVDI KPTNMEELTE VITAAEFHPT ECNVFVYSSS
310 320 330 340 350
KGTIRLCDMR SAALCDRHSK QFEEPENPTN RSFFSEIISS ISDVKLSNSG
360 370 380 390 400
RYMISRDYLS IKVWDLHMET KPIETYPVHE YLRAKLCSLY ENDCIFDKFE
410 420 430 440 450
CCWNGKDSSI MTGSYNNFFR VFDRNSKKDV TLEASRDIIK PKTVLKPRKV
460 470 480 490
CTGGKRKKDE ISVDCLDFNK KILHTAWHPE ENIIAVAATN NLFIFQDKF
Length:499
Mass (Da):56,967
Last modified:June 1, 1994 - v1
Checksum:iD871A7E3058B7286
GO
Isoform B (identifier: P36872-2) [UniParc]FASTAAdd to basket
Also known as: C, D, E, G, H

The sequence of this isoform differs from the canonical sequence as follows:
     1-56: Missing.
     57-59: PAS → MAG

Show »
Length:443
Mass (Da):50,789
Checksum:i83E944093F5BC640
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti211K → M in AAA99871 (PubMed:8382567).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0051051 – 56Missing in isoform B. 2 PublicationsAdd BLAST56
Alternative sequenceiVSP_00510657 – 59PAS → MAG in isoform B. 2 Publications3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13004 mRNA Translation: BAA02367.1
L07581 mRNA Translation: AAA99870.1
L07583 mRNA Translation: AAA99871.1
L07585 Genomic DNA No translation available.
L07586, L12544 Genomic DNA Translation: AAB00371.1
L07586, L12544 Genomic DNA Translation: AAB00372.1
AE014297 Genomic DNA Translation: AAF54498.1
AE014297 Genomic DNA Translation: AAF54499.3
AE014297 Genomic DNA Translation: AAN13455.1
AE014297 Genomic DNA Translation: AAN13456.1
AE014297 Genomic DNA Translation: AAN13457.1
AE014297 Genomic DNA Translation: AAN13458.1
AE014297 Genomic DNA Translation: AAN13459.1
AE014297 Genomic DNA Translation: AAN13460.1
AY061152 mRNA Translation: AAL28700.1
PIRiA45778
RefSeqiNP_001287269.1, NM_001300340.1 [P36872-1]
NP_476880.1, NM_057532.4 [P36872-1]
NP_476881.1, NM_057533.3 [P36872-2]
NP_599111.1, NM_134284.3 [P36872-2]
NP_599112.1, NM_134285.2 [P36872-2]
NP_599113.1, NM_134286.3 [P36872-2]
NP_731451.1, NM_169329.2 [P36872-1]
NP_731452.1, NM_169330.2 [P36872-2]
NP_731453.1, NM_169331.2 [P36872-2]
UniGeneiDm.3826

Genome annotation databases

EnsemblMetazoaiFBtr0082186; FBpp0081664; FBgn0004889 [P36872-1]
FBtr0082187; FBpp0081665; FBgn0004889 [P36872-1]
FBtr0082188; FBpp0081666; FBgn0004889 [P36872-2]
FBtr0082189; FBpp0081667; FBgn0004889 [P36872-2]
FBtr0082190; FBpp0081668; FBgn0004889 [P36872-2]
FBtr0082191; FBpp0081669; FBgn0004889 [P36872-2]
FBtr0082192; FBpp0081670; FBgn0004889 [P36872-2]
FBtr0082193; FBpp0081671; FBgn0004889 [P36872-2]
FBtr0345177; FBpp0311386; FBgn0004889 [P36872-1]
GeneIDi47877
KEGGidme:Dmel_CG6235
UCSCiCG6235-RC d. melanogaster
CG6235-RF d. melanogaster

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry namei2ABA_DROME
AccessioniPrimary (citable) accession number: P36872
Secondary accession number(s): A4V2M9
, A4V2N0, Q9VH21, Q9VH22
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 23, 2018
This is version 179 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health