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Protein

Protein phosphatase PP2A 55 kDa regulatory subunit

Gene

tws

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Could perform a substrate recognition function or could be responsible for targeting the enzyme complex to the appropriate subcellular compartment.

GO - Molecular functioni

  • protein phosphatase regulator activity Source: FlyBase
  • protein serine/threonine phosphatase activity Source: FlyBase

GO - Biological processi

  • centriole replication Source: FlyBase
  • centrosome cycle Source: FlyBase
  • embryonic development via the syncytial blastoderm Source: FlyBase
  • imaginal disc pattern formation Source: UniProtKB
  • maintenance of neuroblast polarity Source: FlyBase
  • metaphase/anaphase transition of mitotic cell cycle Source: FlyBase
  • mitotic cell cycle Source: FlyBase
  • negative regulation of neuroblast proliferation Source: FlyBase
  • peptidyl-serine dephosphorylation Source: GO_Central
  • protein dephosphorylation Source: FlyBase
  • protein stabilization Source: FlyBase
  • regulation of mitotic metaphase/anaphase transition Source: UniProtKB
  • sensory organ development Source: FlyBase
  • Wnt signaling pathway Source: FlyBase

Enzyme and pathway databases

ReactomeiR-DME-2995383. Initiation of Nuclear Envelope Reformation.
R-DME-69231. Cyclin D associated events in G1.
R-DME-69273. Cyclin A/B1/B2 associated events during G2/M transition.
R-DME-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
SignaLinkiP36872.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase PP2A 55 kDa regulatory subunit
Short name:
PR55
Alternative name(s):
Protein phosphatase PP2A regulatory subunit B
Protein twins
Gene namesi
Name:tws
Synonyms:aar, Pp2A-85F
ORF Names:CG6235
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0004889. tws.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000714371 – 499Protein phosphatase PP2A 55 kDa regulatory subunitAdd BLAST499

Proteomic databases

PaxDbiP36872.
PRIDEiP36872.

Expressioni

Gene expression databases

BgeeiFBgn0004889.
ExpressionAtlasiP36872. differential.
GenevisibleiP36872. DM.

Interactioni

Subunit structurei

PP2A exists in several trimeric forms, all of which consist of a core composed of a catalytic subunit associated with a 65 kDa regulatory subunit (PR65) (subunit A). The core complex associates with a third, variable subunit (subunit B), which confers distinct properties to the holoenzyme.

Protein-protein interaction databases

BioGridi71024. 27 interactors.
DIPiDIP-19897N.
IntActiP36872. 34 interactors.
MINTiMINT-896238.
STRINGi7227.FBpp0081665.

Structurei

3D structure databases

ProteinModelPortaliP36872.
SMRiP36872.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati79 – 118WD 1Add BLAST40
Repeati144 – 185WD 2Add BLAST42
Repeati228 – 266WD 3Add BLAST39
Repeati277 – 317WD 4Add BLAST41
Repeati336 – 374WD 5Add BLAST39
Repeati391 – 432WD 6Add BLAST42
Repeati467 – 498WD 7Add BLAST32

Sequence similaritiesi

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1354. Eukaryota.
COG5170. LUCA.
GeneTreeiENSGT00390000006311.
InParanoidiP36872.
KOiK04354.
OMAiSPTWKFT.
OrthoDBiEOG091G09BB.
PhylomeDBiP36872.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiView protein in InterPro
IPR000009. PP2A_PR55.
IPR018067. PP2A_PR55_CS.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
PANTHERiPTHR11871. PTHR11871. 1 hit.
PIRSFiPIRSF037309. PP2A_PR55. 1 hit.
PRINTSiPR00600. PP2APR55.
SMARTiView protein in SMART
SM00320. WD40. 7 hits.
SUPFAMiSSF50978. SSF50978. 3 hits.
PROSITEiView protein in PROSITE
PS01024. PR55_1. 1 hit.
PS01025. PR55_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P36872-1) [UniParc]FASTAAdd to basket
Also known as: F

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRWGRQSPV LEPPDPQMQT TPPPPTLPPR TFMRQSSITK IGNMLNTAIN
60 70 80 90 100
INGAKKPASN GEASWCFSQI KGALDDDVTD ADIISCVEFN HDGELLATGD
110 120 130 140 150
KGGRVVIFQR DPASKAANPR RGEYNVYSTF QSHEPEFDYL KSLEIEEKIN
160 170 180 190 200
KIRWLQQKNP VHFLLSTNDK TVKLWKVSER DKSFGGYNTK EENGLIRDPQ
210 220 230 240 250
NVTALRVPSV KQIPLLVEAS PRRTFANAHT YHINSISVNS DQETFLSADD
260 270 280 290 300
LRINLWHLEV VNQSYNIVDI KPTNMEELTE VITAAEFHPT ECNVFVYSSS
310 320 330 340 350
KGTIRLCDMR SAALCDRHSK QFEEPENPTN RSFFSEIISS ISDVKLSNSG
360 370 380 390 400
RYMISRDYLS IKVWDLHMET KPIETYPVHE YLRAKLCSLY ENDCIFDKFE
410 420 430 440 450
CCWNGKDSSI MTGSYNNFFR VFDRNSKKDV TLEASRDIIK PKTVLKPRKV
460 470 480 490
CTGGKRKKDE ISVDCLDFNK KILHTAWHPE ENIIAVAATN NLFIFQDKF
Length:499
Mass (Da):56,967
Last modified:June 1, 1994 - v1
Checksum:iD871A7E3058B7286
GO
Isoform B (identifier: P36872-2) [UniParc]FASTAAdd to basket
Also known as: C, D, E, G, H

The sequence of this isoform differs from the canonical sequence as follows:
     1-56: Missing.
     57-59: PAS → MAG

Show »
Length:443
Mass (Da):50,789
Checksum:i83E944093F5BC640
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti211K → M in AAA99871 (PubMed:8382567).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0051051 – 56Missing in isoform B. 2 PublicationsAdd BLAST56
Alternative sequenceiVSP_00510657 – 59PAS → MAG in isoform B. 2 Publications3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13004 mRNA. Translation: BAA02367.1.
L07581 mRNA. Translation: AAA99870.1.
L07583 mRNA. Translation: AAA99871.1.
L07585 Genomic DNA. No translation available.
L07586, L12544 Genomic DNA. Translation: AAB00371.1.
L07586, L12544 Genomic DNA. Translation: AAB00372.1.
AE014297 Genomic DNA. Translation: AAF54498.1.
AE014297 Genomic DNA. Translation: AAF54499.3.
AE014297 Genomic DNA. Translation: AAN13455.1.
AE014297 Genomic DNA. Translation: AAN13456.1.
AE014297 Genomic DNA. Translation: AAN13457.1.
AE014297 Genomic DNA. Translation: AAN13458.1.
AE014297 Genomic DNA. Translation: AAN13459.1.
AE014297 Genomic DNA. Translation: AAN13460.1.
AY061152 mRNA. Translation: AAL28700.1.
PIRiA45778.
RefSeqiNP_001287269.1. NM_001300340.1. [P36872-1]
NP_476880.1. NM_057532.4. [P36872-1]
NP_476881.1. NM_057533.3. [P36872-2]
NP_599111.1. NM_134284.3. [P36872-2]
NP_599112.1. NM_134285.2. [P36872-2]
NP_599113.1. NM_134286.3. [P36872-2]
NP_731451.1. NM_169329.2. [P36872-1]
NP_731452.1. NM_169330.2. [P36872-2]
NP_731453.1. NM_169331.2. [P36872-2]
UniGeneiDm.3826.

Genome annotation databases

EnsemblMetazoaiFBtr0082186; FBpp0081664; FBgn0004889. [P36872-1]
FBtr0082187; FBpp0081665; FBgn0004889. [P36872-1]
FBtr0082188; FBpp0081666; FBgn0004889. [P36872-2]
FBtr0082189; FBpp0081667; FBgn0004889. [P36872-2]
FBtr0082190; FBpp0081668; FBgn0004889. [P36872-2]
FBtr0082191; FBpp0081669; FBgn0004889. [P36872-2]
FBtr0082192; FBpp0081670; FBgn0004889. [P36872-2]
FBtr0082193; FBpp0081671; FBgn0004889. [P36872-2]
FBtr0345177; FBpp0311386; FBgn0004889. [P36872-1]
GeneIDi47877.
KEGGidme:Dmel_CG6235.
UCSCiCG6235-RC. d. melanogaster.
CG6235-RF. d. melanogaster.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry namei2ABA_DROME
AccessioniPrimary (citable) accession number: P36872
Secondary accession number(s): A4V2M9
, A4V2N0, Q9VH21, Q9VH22
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 27, 2017
This is version 172 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Caution

It is uncertain whether Met-1, Met-18, Met-33 or Met-44 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families