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Protein

Nitrate reductase [NADH]

Gene

NIA

Organism
Petunia hybrida (Petunia)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activityi

Nitrite + NAD+ + H2O = nitrate + NADH.

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarityNote: Binds 1 FAD per subunit.By similarity
  • hemeBy similarityNote: Binds 1 heme group per subunit.By similarity
  • Mo-molybdopterinBy similarityNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.By similarity

Enzyme regulationi

Regulated by the nitrogen source and controlled by the circadian rhythm.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi187MolybdenumBy similarity1
Metal bindingi570Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi593Iron (heme axial ligand)PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Nitrate assimilation

Keywords - Ligandi

FAD, Flavoprotein, Heme, Iron, Metal-binding, Molybdenum, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductase [NADH] (EC:1.7.1.1)
Short name:
NR
Gene namesi
Name:NIA
OrganismiPetunia hybrida (Petunia)
Taxonomic identifieri4102 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaePetunioideaePetunia

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001660661 – 909Nitrate reductase [NADH]Add BLAST909

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi426InterchainSequence analysis

Keywords - PTMi

Disulfide bond

Expressioni

Developmental stagei

Maximum expression 2 hours after sunrise. Low expression found 2 hours before and 8 hours after sunrise.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP36859.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini535 – 610Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST76
Domaini652 – 764FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST113

Sequence similaritiesi

Belongs to the nitrate reductase family.Curated
Contains 1 cytochrome b5 heme-binding domain.PROSITE-ProRule annotation
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di2.60.40.650. 1 hit.
3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR022407. OxRdtase_Mopterin_BS.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
[Graphical view]
PIRSFiPIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSiPR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
PR00371. FPNCR.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
SSF56524. SSF56524. 1 hit.
SSF63380. SSF63380. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36859-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASVENRQF SHLEPGLSGV VRSFKPRSDS PVRGCNFPLN NELTNFQKKP
60 70 80 90 100
NTTIYLDCSS SEDDDDDDDK NEYLQMIRKG KLEVEPSVHD IRDEGTADNW
110 120 130 140 150
IERNNSMIRL TGKHPFNSEP PLARLMHHGF ITPVPLHYVR NHGPVPKGMW
160 170 180 190 200
DDWTVEVTGL VKRPMKFTME QLVNEFPSRE LPVTLVCAGN RRKEQNMVKQ
210 220 230 240 250
TIGFNWGAAA VSTTVWRGVP LRAILKRCGI YSRTKGALNI CFEGADVLPG
260 270 280 290 300
GGGSKYGTSI KKEFAMDPSR DIIIAYMQNG EKLTPDHGFP LRMIIPGFIG
310 320 330 340 350
GRMVKWLKRI IVTTQESESY YHYKDNRVLP PHVDAELANA EAWWYKPEYI
360 370 380 390 400
INELNINSVI TTPCHEEILP INSWTTQRPY TLRGYSYSGG GKKVTRVEVT
410 420 430 440 450
MDGGETWNVC TVDHPEKPNK YGKYWCWCFW SLEVEVLDLL SAKEIAVRAW
460 470 480 490 500
DETLNTQPEK LIWNVMGMMN NCWFRVKTNV CKPHKGEIGI VFEHPTQPGN
510 520 530 540 550
LSGGWMAKER HLEISAEAPP TLKKSISTPF MNTASKMYSM SEVKKHNSAD
560 570 580 590 600
SAWIIVHGHV YDATRFLKDH PGGIDSILIN AGTDCTEEFD AIHSDKAKKL
610 620 630 640 650
LEDFRIGELI TTGYTSDSSP NNSVHGSSSF SGFLAPIKEL APAVRSVALI
660 670 680 690 700
PREKIPCKLV DKKSISHDVR KFRFALPSED QVLGLPVGKH IFLCAIIDDK
710 720 730 740 750
LCMRAYTPTS TVDEVGYFEL VVKIYFKGIV PKFPNGGQMS QYLDSLPLGA
760 770 780 790 800
FVDVKGPLGH IEYQGRGNFL VHGKRKFAKK LAMLAGGTGI TPVYQVMQAI
810 820 830 840 850
LKDPEDETEM HVVYANRTED DILLKDELDS WAVKLPERVK VWYVVQDSIK
860 870 880 890 900
EGWKYSTGFI TEAVLREHIP LPSQTTLALA CGPPPMIQFA VNPNLEKMGY

DIKDSLLVF
Length:909
Mass (Da):102,376
Last modified:June 1, 1994 - v1
Checksum:i502C706F6E63E706
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13691 Genomic DNA. Translation: AAA33713.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13691 Genomic DNA. Translation: AAA33713.1.

3D structure databases

ProteinModelPortaliP36859.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.60.40.650. 1 hit.
3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR022407. OxRdtase_Mopterin_BS.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
[Graphical view]
PIRSFiPIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSiPR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
PR00371. FPNCR.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
SSF56524. SSF56524. 1 hit.
SSF63380. SSF63380. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNIA_PETHY
AccessioniPrimary (citable) accession number: P36859
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: October 5, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.