Reviewed, UniProtKB/Swiss-Prot P36859 (NIA_PETHY)

Names and origin

Protein names

Recommended name:
    Nitrate reductase [NADH]
      Short name=NR
    EC=1.7.1.1

Gene names

Name:

NIA

Organism

Petunia hybrida (Petunia)

Taxonomic identifier

4102 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Petunioideae › Petunia

Protein attributes

Sequence length	909 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.
Catalytic activity	Nitrite + NAD+ + H2O = nitrate + NADH.
Cofactor	Binds 1 FAD per subunit. Binds 1 heme group per subunit. Binds 1 molybdenum-pterin group per subunit.
Enzyme regulation	Regulated by the nitrogen source and controlled by the circadian rhythm.
Subunit structure	Homodimer By similarity.
Developmental stage	Maximum expression 2 hours after sunrise. Low expression found 2 hours before and 8 hours after sunrise.
Sequence similarities	Belongs to the nitrate reductase family. Contains 1 cytochrome b5 heme-binding domain. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Nitrate assimilation
Ligand	FAD Flavoprotein Heme Iron Metal-binding Molybdenum NAD
Molecular function	Oxidoreductase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrate reductase (NADH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 909	909	Nitrate reductase [NADH]		PRO_0000166066
Regions
	Domain	535 – 610	76	Cytochrome b5 heme-binding
	Domain	652 – 764	113	FAD-binding FR-type
Sites
	Metal binding	187	1	Molybdenum-pterin Potential
	Metal binding	241	1	Molybdenum-pterin Potential
	Metal binding	570	1	Iron (heme axial ligand) By similarity
	Metal binding	593	1	Iron (heme axial ligand) By similarity
Amino acid modifications
	Disulfide bond	426		Interchain Potential

Sequences

	Sequence		Length	Mass (Da)
	P36859-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 502C706F6E63E706	FASTA	909	102,376
	10 20 30 40 50 60 MAASVENRQF SHLEPGLSGV VRSFKPRSDS PVRGCNFPLN NELTNFQKKP NTTIYLDCSS 70 80 90 100 110 120 SEDDDDDDDK NEYLQMIRKG KLEVEPSVHD IRDEGTADNW IERNNSMIRL TGKHPFNSEP 130 140 150 160 170 180 PLARLMHHGF ITPVPLHYVR NHGPVPKGMW DDWTVEVTGL VKRPMKFTME QLVNEFPSRE 190 200 210 220 230 240 LPVTLVCAGN RRKEQNMVKQ TIGFNWGAAA VSTTVWRGVP LRAILKRCGI YSRTKGALNI 250 260 270 280 290 300 CFEGADVLPG GGGSKYGTSI KKEFAMDPSR DIIIAYMQNG EKLTPDHGFP LRMIIPGFIG 310 320 330 340 350 360 GRMVKWLKRI IVTTQESESY YHYKDNRVLP PHVDAELANA EAWWYKPEYI INELNINSVI 370 380 390 400 410 420 TTPCHEEILP INSWTTQRPY TLRGYSYSGG GKKVTRVEVT MDGGETWNVC TVDHPEKPNK 430 440 450 460 470 480 YGKYWCWCFW SLEVEVLDLL SAKEIAVRAW DETLNTQPEK LIWNVMGMMN NCWFRVKTNV 490 500 510 520 530 540 CKPHKGEIGI VFEHPTQPGN LSGGWMAKER HLEISAEAPP TLKKSISTPF MNTASKMYSM 550 560 570 580 590 600 SEVKKHNSAD SAWIIVHGHV YDATRFLKDH PGGIDSILIN AGTDCTEEFD AIHSDKAKKL 610 620 630 640 650 660 LEDFRIGELI TTGYTSDSSP NNSVHGSSSF SGFLAPIKEL APAVRSVALI PREKIPCKLV 670 680 690 700 710 720 DKKSISHDVR KFRFALPSED QVLGLPVGKH IFLCAIIDDK LCMRAYTPTS TVDEVGYFEL 730 740 750 760 770 780 VVKIYFKGIV PKFPNGGQMS QYLDSLPLGA FVDVKGPLGH IEYQGRGNFL VHGKRKFAKK 790 800 810 820 830 840 LAMLAGGTGI TPVYQVMQAI LKDPEDETEM HVVYANRTED DILLKDELDS WAVKLPERVK 850 860 870 880 890 900 VWYVVQDSIK EGWKYSTGFI TEAVLREHIP LPSQTTLALA CGPPPMIQFA VNPNLEKMGY DIKDSLLVF

References

[1]	"Analysis of the petunia nitrate reductase apoenzyme-encoding gene: a first step for sequence modification analysis." Salanoubat M., Ha D.B.D. Gene 128:147-154(1993) [PubMed: 8514183] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. TLRL13. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L13691 Genomic DNA. Translation: AAA33713.1.
3D structure databases
HSSP	HSSP built from PDB template 2CND based on UniProtKB P17571.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.7.1.1. 2263.
Family and domain databases
InterPro	IPR001199. Cyt_B5. IPR018506. Cyt_B5_heme-BS. IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR005066. MoCF_OxRdtse_dimer. IPR008335. Mopterin_OxRdtase_euk. IPR001834. NADH-Cyt_B5_reductase. IPR012137. Nitr_rd_NADH. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. IPR000572. OxRdtase_Mopterin-bd. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit. G3DSA:2.60.40.650. MoCF_oxrdtse_dimer. 1 hit. G3DSA:3.90.420.10. Oxred_molyb_bd. 1 hit.
Pfam	PF00173. Cyt-b5. 1 hit. PF00970. FAD_binding_6. 1 hit. PF03404. Mo-co_dimer. 1 hit. PF00175. NAD_binding_1. 1 hit. PF00174. Oxidored_molyb. 1 hit. [Graphical view]
PIRSF	PIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTS	PR00406. CYTB5RDTASE. PR00363. CYTOCHROMEB5. PR00407. EUMOPTERIN. PR00371. FPNCR.
ProDom	PD000612. Cyt_B5. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. PS51384. FAD_FR. 1 hit. PS00559. MOLYBDOPTERIN_EUK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIA_PETHY

Accession

Primary (citable) accession number: P36859

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	June 16, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)