P36844 (SPIKE_ADE04) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fiber protein
Short name=SPIKE

Alternative name(s):
Protein IV

Gene names

ORF Names:

L5

Organism

Human adenovirus E serotype 4 (HAdV-4) (Human adenovirus 4)

Taxonomic identifier

28280 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Adenoviridae › Mastadenovirus › Human adenovirus E

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Forms spikes that protrude from each vertex of the icosahedral capsid. Interacts with host receptor CXCAR to provide virion initial attachment to target cell. Fiber proteins are shed during virus entry, when virus is still at the cell surface By similarity.
Subunit structure	Homotrimer. Interacts with host receptor CXCAR. Interacts (via N-terminal tail region) with pentons By similarity.
Subcellular location	Virion By similarity. Host nucleus By similarity. Note: Anchored to the pentons, protrudes from the virion surface By similarity.
Induction	Expressed in the late phase of the viral replicative cycle.
Domain	The tail region anchors the fiber to penton base capsomers, whereas the shaft, built from several repeated motifs, allows the knob to protrude from the virion By similarity.
Miscellaneous	All late proteins expressed from the major late promoter are produced by alternative splicing and alternative polyadenylation of the same gene giving rise to non-overlapping ORFs. A leader sequence is present in the N-terminus of all these mRNAs and is recognized by the viral shutoff protein to provide expression although conventional translation via ribosome scanning from the cap has been shut off in the host cell By similarity.
Sequence similarities	Belongs to the adenoviridae fiber family.

Ontologies

Keywords
Biological process	Host-virus interaction Viral attachment to host cell Virus entry into host cell
Cellular component	Host nucleus Virion
Developmental stage	Late protein
Molecular function	Capsid protein
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: InterPro pathogenesis Inferred from electronic annotation. Source: InterPro viral attachment to host cell Inferred from electronic annotation. Source: UniProtKB-KW viral entry into host cell Inferred from electronic annotation. Source: UniProtKB-KW viral infectious cycle Inferred from electronic annotation. Source: InterPro
Cellular_component	host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell viral capsid Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 426	426	Fiber protein		PRO_0000221788

Sequences

	Sequence		Length	Mass (Da)
	P36844 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: F780F5D0CF56D186	FASTA	426	45,643
	10 20 30 40 50 60 MSKSARGWSD GFDPVYPYDA DNDRPCPSST LPSFSSDGFQ EKPLGVLSLG PGRPCHTKNG 70 80 90 100 110 120 EITLKLGEGV DLDDSGKLIA NTVNKAIAPL SFFQQHHFPL TWIPLYTPKM ENYPYKFLPP 130 140 150 160 170 180 LSILKSTILN TLVSAFGSGL GLSGSALAVQ LASPLTFDDK GNIKITLNRG LHVTTGDAIE 190 200 210 220 230 240 SNISWAKGIK FEDGAIATNI GKGSRFGTSS TETGVNNAYP IQVKLGSGLS FDSTGAIMAG 250 260 270 280 290 300 NKDYDKLTLW TTPDPSPNCQ ILAENDAKLT LCLTMCDSQI LATVSVLVVR SGNLNPITGT 310 320 330 340 350 360 VSSAQVFLRF DANGVLLTEH STSKKYWGYK QGDSIDGTPY TNAVGFMPNS TAYPKTQSST 370 380 390 400 410 420 TKNNIVGQVY MNGDVSKPML LTITLNGTDD TTSAYSMSFS YTWTNGSYIG ATFGANSYTF SYIAQQ

References

[1]	Pring-Akerblom P., Adrian T. Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Isolate RJ-67.
[2]	"Adenovirus receptors." Zhang Y., Bergelson J.M. J. Virol. 79:12125-12131(2005) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X76547 Genomic DNA. Translation: CAA54049.1.
PIR	S39300.
3D structure databases
ProteinModelPortal	P36844.
SMR	P36844. Positions 236-426.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.10.25.20. 3 hits. 2.60.90.10. 1 hit.
InterPro	IPR000931. Adeno_fibre. IPR000978. Adeno_fibre_knob. IPR000939. Adenobir_fibre_prot_rpt/shaft. IPR008982. Adenovirus_pIV-rel_att. IPR009013. Attachment_protein_shaft_dom. [Graphical view]
Pfam	PF00541. Adeno_knob. 1 hit. PF00608. Adeno_shaft. 2 hits. [Graphical view]
PRINTS	PR00307. ADENOVSFIBRE.
SUPFAM	SSF49835. Viral_att. 1 hit. SSF51225. Viral_att_shaft. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SPIKE_ADE04

Accession

Primary (citable) accession number: P36844

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program