Reviewed, UniProtKB/Swiss-Prot P36843 (ARGJ_BACSU)

Names and origin

Protein names

Recommended name:
    Arginine biosynthesis bifunctional protein argJ
Cleaved into the following 2 chains:
    1- Recommended name:
            Arginine biosynthesis bifunctional protein argJ alpha chain
    2- Recommended name:
            Arginine biosynthesis bifunctional protein argJ beta chain
Including the following 2 domains:
    1- Recommended name:
            Glutamate N-acetyltransferase
              EC=2.3.1.35
        Alternative name(s):
            Ornithine acetyltransferase
              Short name=OATase
            Ornithine transacetylase
    2- Recommended name:
            Amino-acid acetyltransferase
              EC=2.3.1.1
        Alternative name(s):
            N-acetylglutamate synthase
              Short name=AGS

Gene names

Name:	argJ
Ordered Locus Names:	BSU11200

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of acetylglutamate from glutamate and acetyl-CoA, and of ornithine by transacetylation between acetylornithine and glutamate By similarity.
Catalytic activity	N(2)-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate. HAMAP MF_01106 Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP MF_01106
Enzyme regulation	Feedback inhibition by L-arginine By similarity.
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1. HAMAP MF_01106 Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP MF_01106
Subunit structure	Heterotetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm Probable.
Miscellaneous	Some bacteria possess a monofunctional argJ, i.e., capable of catalyzing only the fifth step of the arginine biosynthetic pathway. HAMAP MF_01106
Sequence similarities	Belongs to the argJ family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	amino-acid N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 192	192	Arginine biosynthesis bifunctional protein argJ alpha chain By similarity		PRO_0000002121
	Chain	193 – 406	214	Arginine biosynthesis bifunctional protein argJ beta chain By similarity		PRO_0000002122
Sites
	Site	192 – 193	2	Cleavage; by autolysis By similarity
Experimental info
	Sequence conflict	181	1	A → R in CAA81544. Ref.1
	Sequence conflict	216	1	I → T in CAA81544. Ref.1
	Sequence conflict	242	1	G → A in CAA81544. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P36843-1 [UniParc]. Last modified June 16, 2009. Version 2. Checksum: 9537998AEF0CAF81	FASTA	406	43,277
	10 20 30 40 50 60 MIQLSEDQIV KVTGDVSSPK GFQAKGVHCG LRYSKKDLGV IISETPAVSA AVYTQSHFQA 70 80 90 100 110 120 APIKVTQDSL KHGPTLKAVI VNSAIANACT GEQGLKDAYT MRESFASQLG IEPELVAVSS 130 140 150 160 170 180 TGVIGEHLDM EKIHAGIELL KETPAGSGDF EEAILTTDTV IKQTCYELAI GGKTVTIGGA 190 200 210 220 230 240 AKGSGMIHPN MATMLGFVTT DAAIEEKALQ KALREITDVS FNQITVDGET STNDMVLVMA 250 260 270 280 290 300 NGCAENECLT EDHPDWPVFK KALLLTCEDL AKEIARDGEG ATKLIEAQVQ GAKNNLDANV 310 320 330 340 350 360 IAKKIVGSNL VKTAVYGTDA NWGRIIGAIG HSAAQVTAEE VEVYLGGQCL FKNNEPQPFS 370 380 390 400 ESIAKEYLEG DEITIVIKMA EGDGNGRAWG CDLTYDYIKI NASYRT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of the citrulline biosynthetic operon argC-F from Bacillus subtilis." O'Reilly M., Devine K.M. Microbiology 140:1023-1025(1994) [PubMed: 8025667] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 0:0-0(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO 181; 216 AND 242.
[4]	"A 10.3 kbp segment from nprB to argJ at the 102 degrees region of the Bacillus subtilis chromosome." Levine A., Vannier F., Roche B., Autret S., Mavel D., Seror S.J. Microbiology 143:175-177(1997) [PubMed: 9025291] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-80. Strain: 168.
[5]	"Sequencing of regions downstream of addA (98 degrees) and citG (289 degrees) in Bacillus subtilis." Medina N., Vannier F., Roche B., Autret S., Levine A., Seror S.J. Microbiology 143:3305-3308(1997) [PubMed: 9353931] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-80. Strain: 168.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z26919 Genomic DNA. Translation: CAA81544.1. AL009126 Genomic DNA. Translation: CAB12961.2. Z79580 Genomic DNA. Translation: CAB01843.1. Y09476 Genomic DNA. Translation: CAA70639.1.
PIR	I40373.
RefSeq	NP_389002.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	T05.001.
Genome annotation databases
GeneID	939800.
GenomeReviews	Gene locus BSU11200 in contig AL009126_GR.
KEGG	bsu:BSU11200.
NMPDR	fig|224308.1.peg.1121.
Organism-specific databases
SubtiList	BG10192. argJ. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P36843.
OMA	P36843. IVNSGNA.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU1121-MON.
BRENDA	2.3.1.1. 150. 2.3.1.35. 150.
Family and domain databases
HAMAP	MF_01106. [Tree]
InterPro	IPR002813. Arg_biosynth_ArgJ. [Graphical view]
PANTHER	PTHR23100. ArgJ. 1 hit.
Pfam	PF01960. ArgJ. 1 hit. [Graphical view]
ProDom	PD004193. ArgJ. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00120. ArgJ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGJ_BACSU

Accession

Primary (citable) accession number: P36843

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 16, 2009
Last modified:	June 16, 2009
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)