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P36842

- NIA_LEPMC

UniProt

P36842 - NIA_LEPMC

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Protein
Nitrate reductase [NADPH]
Gene
NIAD
Organism
Leptosphaeria maculans (Blackleg fungus) (Phoma lingam)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activityi

Nitrite + NADP+ + H2O = nitrate + NADPH.

Cofactori

Binds 1 FAD By similarity.
Binds 1 heme group. The heme group is called cytochrome b-557 By similarity.
Binds 1 molybdenum-molybdopterin (Mo-MPT) cofactor per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi170 – 1701Molybdenum By similarity
Metal bindingi571 – 5711Iron (heme axial ligand) By similarity
Metal bindingi594 – 5941Iron (heme axial ligand) By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi863 – 87210NADP By similarity

GO - Molecular functioni

  1. electron carrier activity Source: InterPro
  2. flavin adenine dinucleotide binding Source: InterPro
  3. heme binding Source: InterPro
  4. iron ion binding Source: InterPro
  5. molybdenum ion binding Source: InterPro
  6. molybdopterin cofactor binding Source: InterPro
  7. nitrate reductase (NADPH) activity Source: UniProtKB-EC

GO - Biological processi

  1. nitrate assimilation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Nitrate assimilation

Keywords - Ligandi

FAD, Flavoprotein, Heme, Iron, Metal-binding, Molybdenum, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductase [NADPH] (EC:1.7.1.3)
Short name:
NR
Gene namesi
Name:NIAD
OrganismiLeptosphaeria maculans (Blackleg fungus) (Phoma lingam)
Taxonomic identifieri5022 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaDothideomycetesPleosporomycetidaePleosporalesPleosporineaeLeptosphaeriaceaeLeptosphaeriaLeptosphaeria maculans complex

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 893893Nitrate reductase [NADPH]
PRO_0000166045Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi418 – 418Interchain Reviewed prediction

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Homodimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliP36842.
SMRiP36842. Positions 61-511.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini536 – 61176Cytochrome b5 heme-binding
Add
BLAST
Domaini641 – 752112FAD-binding FR-type
Add
BLAST

Sequence similaritiesi

Belongs to the nitrate reductase family.

Family and domain databases

Gene3Di2.60.40.650. 1 hit.
3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR022407. OxRdtase_Mopterin_BS.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
[Graphical view]
PIRSFiPIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSiPR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
PR00371. FPNCR.
SUPFAMiSSF55856. SSF55856. 1 hit.
SSF56524. SSF56524. 1 hit.
SSF63380. SSF63380. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36842-1 [UniParc]FASTAAdd to Basket

« Hide

MSVTTQQPAV VPLPSSPRLP IESHAGPRST QLPPSPPETV NGDDPKSITS    50
TPAEPDFPLP PPANPKPQVL DIDKPTPDAH VPRDPRLIRL TGVHPFNTEP 100
PLTDLYNEGF LTSPELFYVR NHGAVPEVQD EECLDWEFSI EGMVANPLKI 150
TLRQLLEEYE NVTYPVTLVC AGNRRKEQNV VRKSKGFAWG AAGVSTALFT 200
GVVMKDVIER AKPLRKAKYV CMEGADKLPN GYYGTSVKLN WVMDPNRGIM 250
LAHKMNGENL SLDHGKPLRA VVPGQIGGRS VKWLKKLIVT AEPSDNWYHI 300
YDNRVLPTMV DPDEAAKNPK WWMDERYAIY DLSPNSAIAF PAHEEKVVLA 350
SAENSYNVRG YAYSGGGRRI TRCEVSLNKG KNWRLANIDY AEDKYRDFEG 400
RELFGARLDM DWRETSFCWC FWNLDIATAE LRDANDILVR AMDEAMCIQP 450
RDMYWSVLGM MNNPWYRITI HHEGDVLRFE HPTQPALIPG GWMERVKKAG 500
GNLTNGQWGE QIEGQELENT AVEEVKEIKM TKDGVNRIVE LDELKWHESA 550
EYPWFVVNDE VYDGTSFLEG HPGGAQSIIS AAGLDASDEF MAIHSETAKA 600
MMPAYHIGTL SPTASKQLSL EEPTSKQASS SSLRPTFLDS RTWSKALLSS 650
KTKVSWDTRI FRFKLDHASQ TLGLPTGQHL MIRLRDPVTR EAIIRSYTPI 700
SQISEQGFCD VLIKIYADAP GREGGKMTKA LDSIPCGHWV DMKGPIGKFE 750
YLGKGVCSIN GNERRVRSMK MICGGSGITP IYQVLRAILQ DSADSTHCTV 800
LNGNRLEEDI LCREDLDRFA EENGERCTLV HTLTQAAEGW TGRRGRIGEE 850
LLKEFCGTEE DGLVLVCGPE GLERSVKGLL SGMAWRDDDV IFF 893
Length:893
Mass (Da):99,942
Last modified:February 1, 1995 - v2
Checksum:i7686E3C9676DD2EF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U04445 Genomic DNA. Translation: AAA50579.1.
U18793 Genomic DNA. Translation: AAA82740.1.
PIRiS46442.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U04445 Genomic DNA. Translation: AAA50579.1 .
U18793 Genomic DNA. Translation: AAA82740.1 .
PIRi S46442.

3D structure databases

ProteinModelPortali P36842.
SMRi P36842. Positions 61-511.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.40.650. 1 hit.
3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProi IPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR022407. OxRdtase_Mopterin_BS.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
[Graphical view ]
PIRSFi PIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSi PR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
PR00371. FPNCR.
SUPFAMi SSF55856. SSF55856. 1 hit.
SSF56524. SSF56524. 1 hit.
SSF63380. SSF63380. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEi PS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nitrate reductase of the ascomycetous fungus, Leptosphaeria maculans: gene sequence and chromosomal location."
    Williams R.S.B., Davis M.A., Howlett B.J.
    Mol. Gen. Genet. 244:1-8(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Isolate 19.
  2. "The nitrate and nitrite reductase-encoding genes of Leptosphaeria maculans are closely linked and transcribed in the same direction."
    Williams R.S.B., Davis M.A., Howlett B.J.
    Gene 158:153-154(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 886-893.
    Strain: Isolate 19.

Entry informationi

Entry nameiNIA_LEPMC
AccessioniPrimary (citable) accession number: P36842
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: February 1, 1995
Last modified: October 16, 2013
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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