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P36842 (NIA_LEPMC) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nitrate reductase [NADPH]

Short name=NR
EC=1.7.1.3
Gene names
Name:NIAD
OrganismLeptosphaeria maculans (Blackleg fungus) (Phoma lingam)
Taxonomic identifier5022 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaDothideomycetesPleosporomycetidaePleosporalesPleosporineaeLeptosphaeriaceaeLeptosphaeriaLeptosphaeria maculans complex

Protein attributes

Sequence length893 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activity

Nitrite + NADP+ + H2O = nitrate + NADPH.

Cofactor

Binds 1 FAD By similarity.

Binds 1 heme group. The heme group is called cytochrome b-557 By similarity.

Binds 1 molybdenum-molybdopterin (Mo-MPT) cofactor per subunit By similarity.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the nitrate reductase family.

Contains 1 cytochrome b5 heme-binding domain.

Contains 1 FAD-binding FR-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 893893Nitrate reductase [NADPH]
PRO_0000166045

Regions

Domain536 – 61176Cytochrome b5 heme-binding
Domain641 – 752112FAD-binding FR-type
Nucleotide binding863 – 87210NADP By similarity

Sites

Metal binding1701Molybdenum By similarity
Metal binding5711Iron (heme axial ligand) By similarity
Metal binding5941Iron (heme axial ligand) By similarity

Amino acid modifications

Disulfide bond418Interchain Potential

Sequences

Sequence LengthMass (Da)Tools
P36842 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: 7686E3C9676DD2EF

FASTA89399,942
        10         20         30         40         50         60 
MSVTTQQPAV VPLPSSPRLP IESHAGPRST QLPPSPPETV NGDDPKSITS TPAEPDFPLP 

        70         80         90        100        110        120 
PPANPKPQVL DIDKPTPDAH VPRDPRLIRL TGVHPFNTEP PLTDLYNEGF LTSPELFYVR 

       130        140        150        160        170        180 
NHGAVPEVQD EECLDWEFSI EGMVANPLKI TLRQLLEEYE NVTYPVTLVC AGNRRKEQNV 

       190        200        210        220        230        240 
VRKSKGFAWG AAGVSTALFT GVVMKDVIER AKPLRKAKYV CMEGADKLPN GYYGTSVKLN 

       250        260        270        280        290        300 
WVMDPNRGIM LAHKMNGENL SLDHGKPLRA VVPGQIGGRS VKWLKKLIVT AEPSDNWYHI 

       310        320        330        340        350        360 
YDNRVLPTMV DPDEAAKNPK WWMDERYAIY DLSPNSAIAF PAHEEKVVLA SAENSYNVRG 

       370        380        390        400        410        420 
YAYSGGGRRI TRCEVSLNKG KNWRLANIDY AEDKYRDFEG RELFGARLDM DWRETSFCWC 

       430        440        450        460        470        480 
FWNLDIATAE LRDANDILVR AMDEAMCIQP RDMYWSVLGM MNNPWYRITI HHEGDVLRFE 

       490        500        510        520        530        540 
HPTQPALIPG GWMERVKKAG GNLTNGQWGE QIEGQELENT AVEEVKEIKM TKDGVNRIVE 

       550        560        570        580        590        600 
LDELKWHESA EYPWFVVNDE VYDGTSFLEG HPGGAQSIIS AAGLDASDEF MAIHSETAKA 

       610        620        630        640        650        660 
MMPAYHIGTL SPTASKQLSL EEPTSKQASS SSLRPTFLDS RTWSKALLSS KTKVSWDTRI 

       670        680        690        700        710        720 
FRFKLDHASQ TLGLPTGQHL MIRLRDPVTR EAIIRSYTPI SQISEQGFCD VLIKIYADAP 

       730        740        750        760        770        780 
GREGGKMTKA LDSIPCGHWV DMKGPIGKFE YLGKGVCSIN GNERRVRSMK MICGGSGITP 

       790        800        810        820        830        840 
IYQVLRAILQ DSADSTHCTV LNGNRLEEDI LCREDLDRFA EENGERCTLV HTLTQAAEGW 

       850        860        870        880        890 
TGRRGRIGEE LLKEFCGTEE DGLVLVCGPE GLERSVKGLL SGMAWRDDDV IFF 

« Hide

References

[1]"Nitrate reductase of the ascomycetous fungus, Leptosphaeria maculans: gene sequence and chromosomal location."
Williams R.S.B., Davis M.A., Howlett B.J.
Mol. Gen. Genet. 244:1-8(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Isolate 19.
[2]"The nitrate and nitrite reductase-encoding genes of Leptosphaeria maculans are closely linked and transcribed in the same direction."
Williams R.S.B., Davis M.A., Howlett B.J.
Gene 158:153-154(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 886-893.
Strain: Isolate 19.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U04445 Genomic DNA. Translation: AAA50579.1.
U18793 Genomic DNA. Translation: AAA82740.1.
PIRS46442.

3D structure databases

ProteinModelPortalP36842.
SMRP36842. Positions 61-511.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.40.650. 1 hit.
3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR022407. OxRdtase_Mopterin_BS.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamPF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
[Graphical view]
PIRSFPIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSPR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
PR00371. FPNCR.
SUPFAMSSF55856. SSF55856. 1 hit.
SSF56524. SSF56524. 1 hit.
SSF63380. SSF63380. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNIA_LEPMC
AccessionPrimary (citable) accession number: P36842
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: February 1, 1995
Last modified: October 16, 2013
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families