Reviewed, UniProtKB/Swiss-Prot P36841 (NIA_VOLCA)

Names and origin

Protein names

Recommended name:
    Nitrate reductase [NADH]
      Short name=NR
    EC=1.7.1.1

Gene names

Name:

NITA

Organism

Volvox carteri

Taxonomic identifier

3067 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Chlorophyta › Chlorophyceae › Chlamydomonadales › Volvocaceae › Volvox

Protein attributes

Sequence length	864 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.
Catalytic activity	Nitrite + NAD+ + H2O = nitrate + NADH.
Cofactor	Binds 1 FAD per subunit. Binds 1 heme group per subunit. Binds 1 molybdenum-pterin group per subunit.
Subunit structure	Homodimer By similarity.
Induction	By nitrate.
Sequence similarities	Belongs to the nitrate reductase family. Contains 1 cytochrome b5 heme-binding domain. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Nitrate assimilation
Ligand	FAD Flavoprotein Heme Iron Metal-binding Molybdenum NAD
Molecular function	Oxidoreductase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrate reductase (NADH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 864	864	Nitrate reductase [NADH]		PRO_0000166074
Regions
	Domain	497 – 572	76	Cytochrome b5 heme-binding
	Domain	606 – 718	113	FAD-binding FR-type
Sites
	Metal binding	139	1	Molybdenum-pterin Potential
	Metal binding	193	1	Molybdenum-pterin Potential
	Metal binding	532	1	Iron (heme axial ligand) By similarity
	Metal binding	555	1	Iron (heme axial ligand) By similarity
Amino acid modifications
	Disulfide bond	376		Interchain Potential

Sequences

	Sequence		Length	Mass (Da)
	P36841-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 499529652CDDD1C7	FASTA	864	96,402
	10 20 30 40 50 60 MTIPELPLGG IVSQAVELGA PYEPPLTPDH PEWKVHVPAA AVDKKDQDTP DNWVRRDPRI 70 80 90 100 110 120 LRLTGRHPLN CEPPMDVLME YGFITPPAVH FVRNHGAAPR IPWAEHRIEI NGLVDKPLFL 130 140 150 160 170 180 TMDELVALPS ITFPCTLVCA GNRRKEENML KKSIGFNWGP CATSTTYWTG VRLRDLLLLA 190 200 210 220 230 240 GIKSPEQGAN FVCFRGPKGE LPRGSDGSYG TSLTYAKAMD PSSDVIIAYK QNHRWLTPDH 250 260 270 280 290 300 GFPVRMIIPG FIGGRMVKWL SEITVTEVES QNFYHFMDNR VLPSHVDEAL AKEEGWWYKP 310 320 330 340 350 360 EFIINDLNIN SAVARPWHDE VVRLDANKPY TMRGYAYAGG GRKIIRCEVS LDDGKTWRLG 370 380 390 400 410 420 DIQRFEKPNE YGKYWCWVHW SLDVMTFDFL NAKEVLLRAW DETMNTQPAI ITWNVMGMMN 430 440 450 460 470 480 NCYYRIKIHP QVDSDGVMGL RFQHPAPVEL GERGNMGWRE EDNLVAQALA AVKEGATAAA 490 500 510 520 530 540 APAAPPPVVA AAANGGPRQY TMEEVAAHNT EESCWFVHGG KVYDATPYLD EHPGGAESIL 550 560 570 580 590 600 IVAGADATDE FNSIHSSKAK AMLAQYYIGD LVASKPAAAG ATVPEPQPVA STSSPAVDPL 610 620 630 640 650 660 VVLNPRQKVK LPLIERIELN RNTRIFRFGL PSPQHRIGLP VGKHVFTYAT INGENVMRAY 670 680 690 700 710 720 TPISGDEELG RLDMLIKVYF ANEHPAFPDG GKMSQHFESL RIGDTVEFKG PLGHFVYDGR 730 740 750 760 770 780 GSYTLNGKLH KHATHMSFVA GGTGITPCYA VIKAALRDPE DKTQISLVFA NNTEEDILLR 790 800 810 820 830 840 EELDELANNH PDRFHLWHTV SQTNSSDWKF STGRVTLEMF KQHLFACSGP ECLALMCGPP 850 860 AMLEHCCVPF LESMGYSKEQ MIHF

References

[1]	"The nitrate reductase-encoding gene of Volvox carteri: map location, sequence and induction kinetics." Gruber H., Goetinck S.D., Kirk D.L., Schmitt R. Gene 120:75-83(1992) [PubMed: 1398126] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: f. Nagariensis / HK10.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X64136 Genomic DNA. Translation: CAA45497.1.
PIR	JC1422.
3D structure databases
HSSP	HSSP built from PDB template 1B5M based on UniProtKB P04166.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.7.1.1. 260611.
Family and domain databases
InterPro	IPR001199. Cyt_B5. IPR018506. Cyt_B5_heme-BS. IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR005066. MoCF_OxRdtse_dimer. IPR008335. Mopterin_OxRdtase_euk. IPR001834. NADH-Cyt_B5_reductase. IPR012137. Nitr_rd_NADH. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. IPR000572. OxRdtase_Mopterin-bd. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit. G3DSA:2.60.40.650. MoCF_oxrdtse_dimer. 1 hit. G3DSA:3.90.420.10. Oxred_molyb_bd. 1 hit.
Pfam	PF00173. Cyt-b5. 1 hit. PF00970. FAD_binding_6. 1 hit. PF03404. Mo-co_dimer. 1 hit. PF00175. NAD_binding_1. 1 hit. PF00174. Oxidored_molyb. 1 hit. [Graphical view]
PIRSF	PIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTS	PR00406. CYTB5RDTASE. PR00363. CYTOCHROMEB5. PR00407. EUMOPTERIN. PR00371. FPNCR.
ProDom	PD000612. Cyt_B5. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. PS51384. FAD_FR. 1 hit. PS00559. MOLYBDOPTERIN_EUK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIA_VOLCA

Accession

Primary (citable) accession number: P36841

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)