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Protein

Nitrate reductase [NADH]

Gene

NITA

Organism
Volvox carteri (Green alga)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activityi

Nitrite + NAD+ + H2O = nitrate + NADH.

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarityNote: Binds 1 FAD per subunit.By similarity
  • hemeBy similarityNote: Binds 1 heme group per subunit.By similarity
  • Mo-molybdopterinBy similarityNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi139MolybdenumBy similarity1
Metal bindingi532Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi555Iron (heme axial ligand)PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Nitrate assimilation

Keywords - Ligandi

FAD, Flavoprotein, Heme, Iron, Metal-binding, Molybdenum, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductase [NADH] (EC:1.7.1.1)
Short name:
NR
Gene namesi
Name:NITA
OrganismiVolvox carteri (Green alga)
Taxonomic identifieri3067 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesVolvocaceaeVolvox

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001660741 – 864Nitrate reductase [NADH]Add BLAST864

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi376InterchainSequence analysis

Keywords - PTMi

Disulfide bond

Expressioni

Inductioni

By nitrate.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP36841.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini497 – 572Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST76
Domaini606 – 718FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST113

Sequence similaritiesi

Belongs to the nitrate reductase family.Curated
Contains 1 cytochrome b5 heme-binding domain.PROSITE-ProRule annotation
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation

Phylogenomic databases

KOiK10534.

Family and domain databases

Gene3Di2.60.40.650. 1 hit.
3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR022407. OxRdtase_Mopterin_BS.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
[Graphical view]
PIRSFiPIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSiPR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
PR00371. FPNCR.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
SSF56524. SSF56524. 1 hit.
SSF63380. SSF63380. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36841-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIPELPLGG IVSQAVELGA PYEPPLTPDH PEWKVHVPAA AVDKKDQDTP
60 70 80 90 100
DNWVRRDPRI LRLTGRHPLN CEPPMDVLME YGFITPPAVH FVRNHGAAPR
110 120 130 140 150
IPWAEHRIEI NGLVDKPLFL TMDELVALPS ITFPCTLVCA GNRRKEENML
160 170 180 190 200
KKSIGFNWGP CATSTTYWTG VRLRDLLLLA GIKSPEQGAN FVCFRGPKGE
210 220 230 240 250
LPRGSDGSYG TSLTYAKAMD PSSDVIIAYK QNHRWLTPDH GFPVRMIIPG
260 270 280 290 300
FIGGRMVKWL SEITVTEVES QNFYHFMDNR VLPSHVDEAL AKEEGWWYKP
310 320 330 340 350
EFIINDLNIN SAVARPWHDE VVRLDANKPY TMRGYAYAGG GRKIIRCEVS
360 370 380 390 400
LDDGKTWRLG DIQRFEKPNE YGKYWCWVHW SLDVMTFDFL NAKEVLLRAW
410 420 430 440 450
DETMNTQPAI ITWNVMGMMN NCYYRIKIHP QVDSDGVMGL RFQHPAPVEL
460 470 480 490 500
GERGNMGWRE EDNLVAQALA AVKEGATAAA APAAPPPVVA AAANGGPRQY
510 520 530 540 550
TMEEVAAHNT EESCWFVHGG KVYDATPYLD EHPGGAESIL IVAGADATDE
560 570 580 590 600
FNSIHSSKAK AMLAQYYIGD LVASKPAAAG ATVPEPQPVA STSSPAVDPL
610 620 630 640 650
VVLNPRQKVK LPLIERIELN RNTRIFRFGL PSPQHRIGLP VGKHVFTYAT
660 670 680 690 700
INGENVMRAY TPISGDEELG RLDMLIKVYF ANEHPAFPDG GKMSQHFESL
710 720 730 740 750
RIGDTVEFKG PLGHFVYDGR GSYTLNGKLH KHATHMSFVA GGTGITPCYA
760 770 780 790 800
VIKAALRDPE DKTQISLVFA NNTEEDILLR EELDELANNH PDRFHLWHTV
810 820 830 840 850
SQTNSSDWKF STGRVTLEMF KQHLFACSGP ECLALMCGPP AMLEHCCVPF
860
LESMGYSKEQ MIHF
Length:864
Mass (Da):96,402
Last modified:June 1, 1994 - v1
Checksum:i499529652CDDD1C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64136 Genomic DNA. Translation: CAA45497.1.
PIRiJC1422.
RefSeqiXP_002955156.1. XM_002955110.1.
UniGeneiVca.1307.

Genome annotation databases

GeneIDi9622111.
KEGGivcn:VOLCADRAFT_76569.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64136 Genomic DNA. Translation: CAA45497.1.
PIRiJC1422.
RefSeqiXP_002955156.1. XM_002955110.1.
UniGeneiVca.1307.

3D structure databases

ProteinModelPortaliP36841.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi9622111.
KEGGivcn:VOLCADRAFT_76569.

Phylogenomic databases

KOiK10534.

Family and domain databases

Gene3Di2.60.40.650. 1 hit.
3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR022407. OxRdtase_Mopterin_BS.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
[Graphical view]
PIRSFiPIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSiPR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
PR00371. FPNCR.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
SSF56524. SSF56524. 1 hit.
SSF63380. SSF63380. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNIA_VOLCA
AccessioniPrimary (citable) accession number: P36841
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 11, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.