Reviewed, UniProtKB/Swiss-Prot P36774 (LON2_MYXXA)

Names and origin

Protein names

Recommended name:
    ATP-dependent protease La 2
    EC=3.4.21.53

Gene names

Name:	lonD
Synonyms:	bsgA

Organism

Myxococcus xanthus

Taxonomic identifier

34 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Myxococcus

Protein attributes

Sequence length	827 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Degrades short-lived regulatory and abnormal proteins. Essential for fruiting body formation and development.
Catalytic activity	Hydrolysis of proteins in presence of ATP.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Developmental stage	Expressed during both vegetative growth and development.
Sequence similarities	Belongs to the peptidase S16 family. Contains 1 Lon domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent peptidase activity Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 827	826	ATP-dependent protease La 2		PRO_0000076143
Regions
	Domain	32 – 225	194	Lon
	Nucleotide binding	379 – 386	8	ATP Potential
	Compositional bias	262 – 276	15	Glu-rich (acidic)
Sites
	Active site	702	1	By similarity
	Active site	745	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P36774-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 558E918A4A965126	FASTA	827	90,438
	10 20 30 40 50 60 MSDEKKKGSA ASAMPTAMAP PGLINKEDIP QVLPILPLRN SVFFPGGVLP LAVGRQKTIA 70 80 90 100 110 120 LIKDAVRDDQ VIGVVTQRRA EEEDPGAADL YTMGTVARIV KLLKMGEDNY SLVVQGLARF 130 140 150 160 170 180 RVVELVQEAP YLKARVDAVE DKTSSENVEV EALGINLKKL AREVIELMPE LPAAATELVE 190 200 210 220 230 240 SITHPGHLAD LIAANVDVPI EEKQAVLETV DLKARMKLVL ELLNRKREIL KLSNKIDSAV 250 260 270 280 290 300 KGEMSKTQRE YYLRQQLKAI KEELGEMGEE EEELDELQER LKKAGLPPDV EKVANKELNR 310 320 330 340 350 360 LKTIPAASSE YTVARTYLDW IADLPWAKIS EDNLDIENAR QQLDKDHFGI KKVKKRILEY 370 380 390 400 410 420 LAVRKLKNDM RGPILCLVGP PGVGKTSLGQ SVAKATGRKF VRLSLGGVRD EAEIRGHRRT 430 440 450 460 470 480 YVGALPGRFI QSMKKAGTKN PVMMLDEIDK LGADFRGDPS AALLEVLDPE QNNTFSDHYL 490 500 510 520 530 540 DVPFDLSKVM FVATANQLDP IPGPLRDRME IIELTGYTFE EKQSIARIHL VPKQLKEHGL 550 560 570 580 590 600 SPDHIDITDE ALLTLTTAYT REAGVRNLER RIADICRAVA VEVAGGKTEK QTINADRVKE 610 620 630 640 650 660 ILGPEMFYSE VAERTEVPGV ATGLAWTAAG GDLLFIEATK MAGKGGMTLT GQLGDVMKES 670 680 690 700 710 720 ATAALSYLRS KAEQLGISPN FLEKTDLHLH FPAGSIPKDG PSAGVTILTA LTSLLTGIRV 730 740 750 760 770 780 RHDTAMTGEA TLRGLVLPVG GIKEKVLAAH RAGIKRVILP ERCRKDLIDV PDQARNELEF 790 800 810 820 IFVTHMDDVL KAALETPPVG VAGTPGGEPG KEAPLPKPAE SAPEVRA

References

[1]	"The lonD gene is homologous to the lon gene encoding an ATP-dependent protease and is essential for the development of Myxococcus xanthus." Tojo N., Inouye S., Komano T. J. Bacteriol. 175:4545-4549(1993) [PubMed: 8331083] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DZF1.
[2]	"Myxococcus xanthus encodes an ATP-dependent protease which is required for developmental gene transcription and intercellular signaling." Gill R.E., Karlok M., Benton D. J. Bacteriol. 175:4538-4544(1993) [PubMed: 8331082] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-10. Strain: M102.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D13204 Genomic DNA. Translation: BAA02491.1. L19301 Unassigned DNA. Translation: AAA72018.1.
PIR	A36895.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.4.21.53. 926.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR008269. Pept_S16_C. IPR004815. Pept_S16_lon. IPR003111. Pept_S16_N. IPR008268. Peptidase_S16_AS. IPR001984. Peptidase_S16_C. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF02190. LON. 1 hit. PF05362. Lon_C. 1 hit. [Graphical view]
PRINTS	PR00830. ENDOLAPTASE.
SMART	SM00382. AAA. 1 hit. SM00464. LON. 1 hit. [Graphical view]
TIGRFAMs	TIGR00763. lon. 1 hit.
PROSITE	PS01046. LON_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LON2_MYXXA

Accession

Primary (citable) accession number: P36774

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 62 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)