P36711 (FIBP_ADE12) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fiber protein

Alternative name(s):
pIV

Gene names

Name:

PIV

Organism

Human adenovirus A serotype 12 (HAdV-12) (Human adenovirus 12)

Taxonomic identifier

28282 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Adenoviridae › Mastadenovirus › Human adenovirus A

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	587 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function

Forms an icosahedral capsid with a 80-110 nm diameter together with the two other structural proteins hexon and penton. The fiber is divided into the tail, the shaft and the knob. The tails anchors the fiber to penton base capsomers, whereas the shaft, built from several repeated motifs, allows the knob to protude from the virion and interacts with host receptor CXCAR at the cell surface to provide virion attachment to target cell. Heparan sulfate has also been identified as a major attachment receptor. Adenovirus exploits its receptor for two important but distinct steps in its life cycle: attachment to host cells and escape across epithelial barriers to the environment. After virus has been endocytosed, fiber protein seems to modulate viral rapid escape from host endosome via organellar membrane lysis By similarity.

Subunit structure

Homotrimer. Interacts with host receptor CXCAR.

Ontologies

Keywords
Biological process	Host-virus interaction Initiation of viral infection Viral attachment to host cell
Technical term	3D-structure
Gene Ontology (GO)
Biological process	cell adhesion Inferred from electronic annotation. Source: InterPro pathogenesis Inferred from electronic annotation. Source: InterPro virion attachment to host cell surface receptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 587

587

Fiber protein

PRO_0000221795

Experimental info

Mutagenesis

417

1

P → E: Loss of interaction with CXADR. Ref.2

Mutagenesis

418

1

P → E: Loss of interaction with CXADR. Ref.2

Mutagenesis

421

1

S → TIS: Loss of interaction with CXADR. Ref.2

Mutagenesis

425

1

Missing: Loss of interaction with CXADR. Ref.2

Mutagenesis

426

1

Missing: Loss of interaction with CXADR. Ref.2

Mutagenesis

550

1

Missing: Loss of interaction with CXADR. Ref.2

Mutagenesis

551

1

Missing: Loss of interaction with CXADR. Ref.2

Secondary structure

1

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587

Helix Strand Turn

Details...

Beta strand

405 – 412

8

Beta strand

422 – 424

3

Beta strand

428 – 437

10

Beta strand

440 – 449

10

Helix

452 – 455

4

Beta strand

463 – 470

8

Beta strand

482 – 484

3

Beta strand

491 – 494

4

Helix

506 – 508

3

Turn

513 – 515

3

Helix

518 – 520

3

Helix

524 – 526

3

Beta strand

527 – 533

7

Helix

534 – 536

3

Beta strand

540 – 551

12

Beta strand

557 – 564

8

Helix

566 – 568

3

Beta strand

579 – 585

7

Sequences

	Sequence		Length	Mass (Da)
	P36711 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 6D285B5B61008A4C	FASTA	587	61,699
	10 20 30 40 50 60 MKRSRTQYAE ETEENDDFNP VYPFDPFDTS DVPFVTPPFT SSNGLQEKPP GVLALNYKDP 70 80 90 100 110 120 IVTENGTLTL KLGDGIKLNA QGQLTASNNI NVLEPLTNTS QGLKLSWSAP LAVKASALTL 130 140 150 160 170 180 NTRAPLTTTD ESLALITAPP ITVESSRLGL ATIAPLSLDG GGNLGLNLSA PLDVSNNNLH 190 200 210 220 230 240 LTTETPLVVN SSGALSVATA DPISVRNNAL TLPTADPLMV SSDGLGISVT SPITVINGSL 250 260 270 280 290 300 ALSTTAPLNS TGSTLSLSVA NPLTISQDTL TVSTGNGLQV SGSQLVTRIG DGLTFDNGVM 310 320 330 340 350 360 KVNVAGGMRT SGGRIILDVN YPFDASNNLS LRRGLGLIYN QSTNWNLTTD ISTEKGLMFS 370 380 390 400 410 420 GNQIALNAGQ GLTFNNGQLR VKLGAGLIFD SNNNIALGSS SNTPYDPLTL WTTPDPPPNC 430 440 450 460 470 480 SLIQELDAKL TLCLTKNGSI VNGIVSLVGV KGNLLNIQST TTTVGVHLVF DEQGRLITST 490 500 510 520 530 540 PTALVPQASW GYRQGQSVST NTVTNGLGFM PNVSAYPRPN ASEAKSQMVS LTYLQGDTSK 550 560 570 580 PITMKVAFNG ITSLNGYSLT FMWSGLSNYI NQPFSTPSCS FSYITQE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of human adenovirus type 12 DNA: comparative functional analysis." Sprengel J., Schmitz B., Heuss-Neitzel D., Zock C., Doerfler W. J. Virol. 68:379-389(1994) [PubMed: 8254750] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Structural analysis of the mechanism of adenovirus binding to its human cellular receptor, CAR." Bewley M.C., Springer K., Zhang Y.-B., Freimuth P., Flanagan J.M. Science 286:1579-1583(1999) [PubMed: 10567268] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 403-587 IN COMPLEX WITH CXADR, MUTAGENESIS OF PRO-417; PRO-418; SER-421; GLU-425; LEU-426; GLY-550 AND ILE-551.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X73487 Genomic DNA. Translation: CAA51900.1.

PIR

S33951.

RefSeq

NP_040933.1. NC_001460.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KAC	X-ray	2.60	A	403-587	[»]
1NOB	X-ray	2.60	A/B/C/D/E/F	403-587	[»]
1P69	X-ray	3.10	A	403-587	[»]
1P6A	X-ray	2.90	A	403-587	[»]

ProteinModelPortal

P36711.

SMR

P36711. Positions 403-587.

ModBase

Search...

Protein-protein interaction databases

MINT

MINT-110997.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1460847.

Family and domain databases

InterPro

IPR000931. Adeno_fibre.
IPR000978. Adeno_fibre_knob.
IPR000939. Adenobir_fibre_prot_rpt/shaft.
IPR008982. Adenovirus_pIV-rel_att.
IPR009013. Adenovirus_pIV_fibre.
[Graphical view]

Gene3D

G3DSA:2.60.90.10. Adeno_fiber_knob. 1 hit.

Pfam

PF00541. Adeno_knob. 1 hit.
PF00608. Adeno_shaft. 7 hits.
[Graphical view]

PRINTS

PR00307. ADENOVSFIBRE.

SUPFAM

SSF49835. Viral_att. 1 hit.
SSF51225. Viral_att_shaft. 5 hits.

ProtoNet

Search...

Entry information

Entry name

FIBP_ADE12

Accession

Primary (citable) accession number: P36711

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	December 14, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program