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Protein

Fiber protein

Gene

L5

Organism
Human adenovirus A serotype 12 (HAdV-12) (Human adenovirus 12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Forms spikes that protrude from each vertex of the icosahedral capsid. Interacts with host receptor CXCAR to provide virion initial attachment to target cell. Fiber proteins are shed during virus entry, when virus is still at the cell surface (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Viral attachment to host adhesion receptor, Viral attachment to host cell, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Fiber protein
Short name:
SPIKE
Alternative name(s):
Protein IV
Gene namesi
ORF Names:L5
OrganismiHuman adenovirus A serotype 12 (HAdV-12) (Human adenovirus 12)
Taxonomic identifieri28282 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAdenoviridaeMastadenovirusHuman mastadenovirus A
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000004993 Componenti: Genome

Subcellular locationi

  • Virion By similarity
  • Host nucleus By similarity

  • Note: Anchored to the pentons, protrudes from the virion surface.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host nucleus, Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi417P → E: Loss of interaction with CXADR. 1 Publication1
Mutagenesisi418P → E: Loss of interaction with CXADR. 1 Publication1
Mutagenesisi421S → TIS: Loss of interaction with CXADR. 1 Publication1
Mutagenesisi425Missing : Loss of interaction with CXADR. 1 Publication1
Mutagenesisi426Missing : Loss of interaction with CXADR. 1 Publication1
Mutagenesisi550Missing : Loss of interaction with CXADR. 1 Publication1
Mutagenesisi551Missing : Loss of interaction with CXADR. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002217951 – 587Fiber proteinAdd BLAST587

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle.

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Homotrimer. Interacts with host receptor CXCAR. Interacts (via N-terminal tail region) with pentons (By similarity).By similarity

Protein-protein interaction databases

MINTiMINT-110997.

Structurei

Secondary structure

1587
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi405 – 412Combined sources8
Beta strandi422 – 424Combined sources3
Beta strandi428 – 437Combined sources10
Beta strandi440 – 449Combined sources10
Helixi452 – 455Combined sources4
Beta strandi463 – 470Combined sources8
Turni472 – 474Combined sources3
Beta strandi482 – 484Combined sources3
Beta strandi491 – 494Combined sources4
Helixi506 – 508Combined sources3
Turni513 – 515Combined sources3
Helixi518 – 520Combined sources3
Helixi524 – 526Combined sources3
Beta strandi527 – 533Combined sources7
Helixi534 – 536Combined sources3
Beta strandi540 – 551Combined sources12
Beta strandi557 – 564Combined sources8
Helixi566 – 568Combined sources3
Beta strandi579 – 585Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KACX-ray2.60A403-587[»]
1NOBX-ray2.60A/B/C/D/E/F403-587[»]
1P69X-ray3.10A403-587[»]
1P6AX-ray2.90A403-587[»]
ProteinModelPortaliP36711.
SMRiP36711.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP36711.

Family & Domainsi

Domaini

The tail region anchors the fiber to penton base capsomers, whereas the shaft, built from several repeated motifs, allows the knob to protrude from the virion.By similarity

Sequence similaritiesi

Belongs to the adenoviridae fiber family.Curated

Phylogenomic databases

KOiK19262.

Family and domain databases

Gene3Di2.10.25.20. 5 hits.
2.60.90.10. 1 hit.
InterProiIPR000931. Adeno_fibre.
IPR000978. Adeno_fibre_knob.
IPR000939. Adenobir_fibre_prot_rpt/shaft.
IPR008982. Adenovirus_pIV-rel_att.
IPR009013. Attachment_protein_shaft_dom.
[Graphical view]
PfamiPF00541. Adeno_knob. 1 hit.
PF00608. Adeno_shaft. 9 hits.
[Graphical view]
PRINTSiPR00307. ADENOVSFIBRE.
SUPFAMiSSF49835. SSF49835. 1 hit.
SSF51225. SSF51225. 5 hits.

Sequencei

Sequence statusi: Complete.

P36711-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRSRTQYAE ETEENDDFNP VYPFDPFDTS DVPFVTPPFT SSNGLQEKPP
60 70 80 90 100
GVLALNYKDP IVTENGTLTL KLGDGIKLNA QGQLTASNNI NVLEPLTNTS
110 120 130 140 150
QGLKLSWSAP LAVKASALTL NTRAPLTTTD ESLALITAPP ITVESSRLGL
160 170 180 190 200
ATIAPLSLDG GGNLGLNLSA PLDVSNNNLH LTTETPLVVN SSGALSVATA
210 220 230 240 250
DPISVRNNAL TLPTADPLMV SSDGLGISVT SPITVINGSL ALSTTAPLNS
260 270 280 290 300
TGSTLSLSVA NPLTISQDTL TVSTGNGLQV SGSQLVTRIG DGLTFDNGVM
310 320 330 340 350
KVNVAGGMRT SGGRIILDVN YPFDASNNLS LRRGLGLIYN QSTNWNLTTD
360 370 380 390 400
ISTEKGLMFS GNQIALNAGQ GLTFNNGQLR VKLGAGLIFD SNNNIALGSS
410 420 430 440 450
SNTPYDPLTL WTTPDPPPNC SLIQELDAKL TLCLTKNGSI VNGIVSLVGV
460 470 480 490 500
KGNLLNIQST TTTVGVHLVF DEQGRLITST PTALVPQASW GYRQGQSVST
510 520 530 540 550
NTVTNGLGFM PNVSAYPRPN ASEAKSQMVS LTYLQGDTSK PITMKVAFNG
560 570 580
ITSLNGYSLT FMWSGLSNYI NQPFSTPSCS FSYITQE
Length:587
Mass (Da):61,699
Last modified:June 1, 1994 - v1
Checksum:i6D285B5B61008A4C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73487 Genomic DNA. Translation: CAA51900.1.
PIRiS33951.
RefSeqiNP_040933.1. NC_001460.1.

Genome annotation databases

GeneIDi1460847.
KEGGivg:1460847.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73487 Genomic DNA. Translation: CAA51900.1.
PIRiS33951.
RefSeqiNP_040933.1. NC_001460.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KACX-ray2.60A403-587[»]
1NOBX-ray2.60A/B/C/D/E/F403-587[»]
1P69X-ray3.10A403-587[»]
1P6AX-ray2.90A403-587[»]
ProteinModelPortaliP36711.
SMRiP36711.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-110997.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1460847.
KEGGivg:1460847.

Phylogenomic databases

KOiK19262.

Miscellaneous databases

EvolutionaryTraceiP36711.

Family and domain databases

Gene3Di2.10.25.20. 5 hits.
2.60.90.10. 1 hit.
InterProiIPR000931. Adeno_fibre.
IPR000978. Adeno_fibre_knob.
IPR000939. Adenobir_fibre_prot_rpt/shaft.
IPR008982. Adenovirus_pIV-rel_att.
IPR009013. Attachment_protein_shaft_dom.
[Graphical view]
PfamiPF00541. Adeno_knob. 1 hit.
PF00608. Adeno_shaft. 9 hits.
[Graphical view]
PRINTSiPR00307. ADENOVSFIBRE.
SUPFAMiSSF49835. SSF49835. 1 hit.
SSF51225. SSF51225. 5 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiSPIKE_ADE12
AccessioniPrimary (citable) accession number: P36711
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 30, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

All late proteins expressed from the major late promoter are produced by alternative splicing and alternative polyadenylation of the same gene giving rise to non-overlapping ORFs. A leader sequence is present in the N-terminus of all these mRNAs and is recognized by the viral shutoff protein to provide expression although conventional translation via ribosome scanning from the cap has been shut off in the host cell (By similarity).By similarity

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.