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Protein

Cell division protein ZapD

Gene

zapD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cell division factor that enhances FtsZ-ring assembly. Directly interacts with FtsZ and promotes bundling of FtsZ protofilaments, with a reduction in FtsZ GTPase activity.UniRule annotation1 Publication

GO - Molecular functioni

  • identical protein binding Source: IntAct

GO - Biological processi

  • FtsZ-dependent cytokinesis Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Septation

Enzyme and pathway databases

BioCyciEcoCyc:EG12313-MONOMER.
ECOL316407:JW0099-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division protein ZapDUniRule annotation
Alternative name(s):
Z ring-associated protein DUniRule annotation
Gene namesi
Name:zapDUniRule annotation
Synonyms:yacF
Ordered Locus Names:b0102, JW0099
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12313. zapD.

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication

  • Note: Localizes to mid-cell in an FtsZ-dependent manner. Localization does not require FtsA, ZipA, ZapA or ZapC.

GO - Cellular componenti

  • cell division site Source: EcoCyc
  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mutants are viable, with no discernible division phenotypes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 247247Cell division protein ZapDPRO_0000211667Add
BLAST

Proteomic databases

PaxDbiP36680.
PRIDEiP36680.

Interactioni

Subunit structurei

Mostly homodimer in solution. Interacts with the C-terminal tail of FtsZ.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-1113728,EBI-1113728
ftsZP0A9A63EBI-1113728,EBI-370963

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4260784. 2 interactions.
IntActiP36680. 5 interactions.
STRINGi511145.b0102.

Structurei

Secondary structure

1
247
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 127Combined sources
Helixi13 – 2816Combined sources
Helixi37 – 5519Combined sources
Helixi59 – 7618Combined sources
Beta strandi78 – 803Combined sources
Helixi85 – 10420Combined sources
Helixi110 – 1145Combined sources
Helixi116 – 1249Combined sources
Turni128 – 1314Combined sources
Turni133 – 1353Combined sources
Helixi137 – 1426Combined sources
Helixi147 – 15913Combined sources
Helixi162 – 17514Combined sources
Beta strandi182 – 1876Combined sources
Beta strandi190 – 1945Combined sources
Beta strandi199 – 2046Combined sources
Helixi207 – 2093Combined sources
Beta strandi211 – 2188Combined sources
Beta strandi221 – 23313Combined sources
Beta strandi240 – 2467Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5DKOX-ray2.40A1-247[»]
ProteinModelPortaliP36680.
SMRiP36680. Positions 7-247.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ZapD family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DHA. Bacteria.
COG4582. LUCA.
HOGENOMiHOG000283874.
KOiK18778.
OMAiLPAYYAW.

Family and domain databases

Gene3Di2.60.440.10. 1 hit.
HAMAPiMF_01092. ZapD. 1 hit.
InterProiIPR009777. ZapD.
IPR027462. ZapD_C.
[Graphical view]
PfamiPF07072. DUF1342. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36680-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQTQVLFEHP LNEKMRTWLR IEFLIQQLTV NLPIVDHAGA LHFFRNVSEL
60 70 80 90 100
LDVFERGEVR TELLKELDRQ QRKLQTWIGV PGVDQSRIEA LIQQLKAAGS
110 120 130 140 150
VLISAPRIGQ FLREDRLIAL VRQRLSIPGG CCSFDLPTLH IWLHLPQAQR
160 170 180 190 200
DSQVETWIAS LNPLTQALTM VLDLIRQSAP FRKQTSLNGF YQDNGGDADL
210 220 230 240
LRLNLSLDSQ LYPQISGHKS RFAIRFMPLD TENGQVPERL DFELACC
Length:247
Mass (Da):28,292
Last modified:November 1, 1997 - v2
Checksum:i3C0B917A7FA844AC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti215 – 2151I → T no nucleotide entry (PubMed:8202364).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73213.1.
AP009048 Genomic DNA. Translation: BAB96671.2.
PIRiF64732.
RefSeqiNP_414644.1. NC_000913.3.
WP_001194734.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73213; AAC73213; b0102.
BAB96671; BAB96671; BAB96671.
GeneIDi944873.
KEGGiecj:JW0099.
eco:b0102.
PATRICi32115307. VBIEscCol129921_0105.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73213.1.
AP009048 Genomic DNA. Translation: BAB96671.2.
PIRiF64732.
RefSeqiNP_414644.1. NC_000913.3.
WP_001194734.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5DKOX-ray2.40A1-247[»]
ProteinModelPortaliP36680.
SMRiP36680. Positions 7-247.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260784. 2 interactions.
IntActiP36680. 5 interactions.
STRINGi511145.b0102.

Proteomic databases

PaxDbiP36680.
PRIDEiP36680.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73213; AAC73213; b0102.
BAB96671; BAB96671; BAB96671.
GeneIDi944873.
KEGGiecj:JW0099.
eco:b0102.
PATRICi32115307. VBIEscCol129921_0105.

Organism-specific databases

EchoBASEiEB2219.
EcoGeneiEG12313. zapD.

Phylogenomic databases

eggNOGiENOG4105DHA. Bacteria.
COG4582. LUCA.
HOGENOMiHOG000283874.
KOiK18778.
OMAiLPAYYAW.

Enzyme and pathway databases

BioCyciEcoCyc:EG12313-MONOMER.
ECOL316407:JW0099-MONOMER.

Miscellaneous databases

PROiP36680.

Family and domain databases

Gene3Di2.60.440.10. 1 hit.
HAMAPiMF_01092. ZapD. 1 hit.
InterProiIPR009777. ZapD.
IPR027462. ZapD_C.
[Graphical view]
PfamiPF07072. DUF1342. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZAPD_ECOLI
AccessioniPrimary (citable) accession number: P36680
Secondary accession number(s): P75645, Q8KJQ6, Q8KMZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.